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The map label for this gene is leuB [H]

Identifier: 73541997

GI number: 73541997

Start: 2550320

End: 2551387

Strand: Reverse

Name: leuB [H]

Synonym: Reut_A2311

Alternate gene names: 73541997

Gene position: 2551387-2550320 (Counterclockwise)

Preceding gene: 73541998

Following gene: 73541996

Centisome position: 67.03

GC content: 65.07

Gene sequence:

>1068_bases
ATGAAGATCGCAGTCCTGCCAGGTGACGGCATCGGTCCGGAAATCGTTGCCGAGGCCGTCAAGGTCCTGAACGCGCTCGA
CGAGAAGTTCGAGATGGAAACCGCCCCGGTGGGCGGCGCCGGCTACGAGGCCGAAGGCCACCCACTGCCGGAGAACACGC
TGAAGCTGGCCAAGGAAGCCGATGCCATCCTGTTCGGCGCCGTGGGCGACTGGAAGTACGACAGCCTGGAGCGCGCGCTG
CGCCCCGAGCAGGCCATCCTGGGCCTGCGCAAGCACCTGCAGCTGTTCGCCAACTTCCGCCCGGCGATCTGCTATCCGGA
ATTGACGGGCGCATCGAGCCTGAAGCCGGAACTGGTCGCGGGCCTGGACATCCTGATCGTGCGCGAACTGAATGGCGACA
TTTACTTCGGCCAGCCGCGCGGCGTGCGTGAGGCGCCGGACGGCCTGTTCAAGGGCGCCCGCGAAGGTTTCGACACCATG
CGCTACAGCGAGCCGGAAATCCGCCGCATCGCGCATGTGGCGTTCCAGGCCGCGGCCAAGCGCGGCAAGAAGCTGTGCAG
CGTCGACAAGGCCAACGTGCTCGAGACCTTTCAGTTCTGGAAGGACATCGTGATCGATGTCAGCAAGGAGTATCCGGACG
TCGAGCTGTCGCACATGTACGTCGACAACGCCGCGATGCAGCTGGTCAAGGCGCCCAAGAGCTTCGATGTAATCGTCACC
GGCAATATGTTCGGCGACATCCTGTCGGACGAGGCCGCGATGCTGACCGGCTCGATCGGCATGCTGCCGTCGGCCTCGCT
GGATGCGAACAACAAGGGCCTGTACGAGCCGTCGCACGGTTCGGCCCCGGACATCGCGGGCAAGGGCGTCGCCAATCCGC
TGGCGACCATCCTGTCGGCGGCGATGATGCTGCGCTACTCGCTGAACAAGGCCGAACAGGCCGACCGCATCGAGAACGCC
GTGAAGAAGGTGCTGGCACAGGGCTATCGCACCGGCGACATCCTGACGCCGGGCTGCAAGCAGGTCGGCACGCGCGAAAT
GGGCGAGGCCGTACTGGCCGCTCTGTAA

Upstream 100 bases:

>100_bases
TCAAGAGCTATGAAGCCGAGCGCGTGGCGAAGATGCCGTGGCTGAACAACCGCGTGGTCGGCTGAACGCCACAGCGGAAA
TCAAACAAGGAAAACACAGC

Downstream 100 bases:

>100_bases
GTCACAATCCGGAAAAGGCGCTGCGCTGCAGCGCCTTTTCTTTTATTTGTCCCCGATTTTCCACGCTGCGGCACCGCCGC
AAAATCGTGTAGACTCTGCG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKIAVLPGDGIGPEIVAEAVKVLNALDEKFEMETAPVGGAGYEAEGHPLPENTLKLAKEADAILFGAVGDWKYDSLERAL
RPEQAILGLRKHLQLFANFRPAICYPELTGASSLKPELVAGLDILIVRELNGDIYFGQPRGVREAPDGLFKGAREGFDTM
RYSEPEIRRIAHVAFQAAAKRGKKLCSVDKANVLETFQFWKDIVIDVSKEYPDVELSHMYVDNAAMQLVKAPKSFDVIVT
GNMFGDILSDEAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGVANPLATILSAAMMLRYSLNKAEQADRIENA
VKKVLAQGYRTGDILTPGCKQVGTREMGEAVLAAL

Sequences:

>Translated_355_residues
MKIAVLPGDGIGPEIVAEAVKVLNALDEKFEMETAPVGGAGYEAEGHPLPENTLKLAKEADAILFGAVGDWKYDSLERAL
RPEQAILGLRKHLQLFANFRPAICYPELTGASSLKPELVAGLDILIVRELNGDIYFGQPRGVREAPDGLFKGAREGFDTM
RYSEPEIRRIAHVAFQAAAKRGKKLCSVDKANVLETFQFWKDIVIDVSKEYPDVELSHMYVDNAAMQLVKAPKSFDVIVT
GNMFGDILSDEAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGVANPLATILSAAMMLRYSLNKAEQADRIENA
VKKVLAQGYRTGDILTPGCKQVGTREMGEAVLAAL
>Mature_355_residues
MKIAVLPGDGIGPEIVAEAVKVLNALDEKFEMETAPVGGAGYEAEGHPLPENTLKLAKEADAILFGAVGDWKYDSLERAL
RPEQAILGLRKHLQLFANFRPAICYPELTGASSLKPELVAGLDILIVRELNGDIYFGQPRGVREAPDGLFKGAREGFDTM
RYSEPEIRRIAHVAFQAAAKRGKKLCSVDKANVLETFQFWKDIVIDVSKEYPDVELSHMYVDNAAMQLVKAPKSFDVIVT
GNMFGDILSDEAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGVANPLATILSAAMMLRYSLNKAEQADRIENA
VKKVLAQGYRTGDILTPGCKQVGTREMGEAVLAAL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=329, Percent_Identity=31.9148936170213, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI28178816, Length=368, Percent_Identity=28.2608695652174, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI28178821, Length=332, Percent_Identity=28.3132530120482, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI4758582, Length=306, Percent_Identity=27.7777777777778, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI28178838, Length=321, Percent_Identity=28.0373831775701, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI28178819, Length=155, Percent_Identity=34.8387096774194, Blast_Score=90, Evalue=2e-18,
Organism=Escherichia coli, GI87081683, Length=355, Percent_Identity=52.9577464788732, Blast_Score=352, Evalue=2e-98,
Organism=Escherichia coli, GI1788101, Length=349, Percent_Identity=33.810888252149, Blast_Score=168, Evalue=6e-43,
Organism=Escherichia coli, GI1787381, Length=398, Percent_Identity=27.1356783919598, Blast_Score=88, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI71986051, Length=336, Percent_Identity=31.8452380952381, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI25144293, Length=337, Percent_Identity=30.8605341246291, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17505779, Length=335, Percent_Identity=29.5522388059701, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17550882, Length=338, Percent_Identity=29.585798816568, Blast_Score=115, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=365, Percent_Identity=46.5753424657534, Blast_Score=278, Evalue=7e-76,
Organism=Saccharomyces cerevisiae, GI6322097, Length=371, Percent_Identity=35.5795148247978, Blast_Score=166, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6324291, Length=333, Percent_Identity=30.6306306306306, Blast_Score=126, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6324709, Length=367, Percent_Identity=29.700272479564, Blast_Score=123, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24643270, Length=341, Percent_Identity=32.8445747800587, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24643268, Length=341, Percent_Identity=32.8445747800587, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24661184, Length=346, Percent_Identity=30.0578034682081, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI161078635, Length=336, Percent_Identity=26.1904761904762, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI161078633, Length=336, Percent_Identity=26.1904761904762, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24650122, Length=336, Percent_Identity=26.1904761904762, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI161078637, Length=336, Percent_Identity=26.1904761904762, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI161078639, Length=334, Percent_Identity=26.3473053892216, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI281362242, Length=353, Percent_Identity=28.8951841359773, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648872, Length=353, Percent_Identity=28.8951841359773, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI20130355, Length=172, Percent_Identity=31.3953488372093, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38303; Mature: 38303

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAVLPGDGIGPEIVAEAVKVLNALDEKFEMETAPVGGAGYEAEGHPLPENTLKLAKEA
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHC
DAILFGAVGDWKYDSLERALRPEQAILGLRKHLQLFANFRPAICYPELTGASSLKPELVA
CEEEEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHH
GLDILIVRELNGDIYFGQPRGVREAPDGLFKGAREGFDTMRYSEPEIRRIAHVAFQAAAK
CCEEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RGKKLCSVDKANVLETFQFWKDIVIDVSKEYPDVELSHMYVDNAAMQLVKAPKSFDVIVT
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEHHEEHHHHHHHHHHCCCCCEEEEE
GNMFGDILSDEAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGVANPLATILSA
CCHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AMMLRYSLNKAEQADRIENAVKKVLAQGYRTGDILTPGCKQVGTREMGEAVLAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCHHHHHHHHHHCC
>Mature Secondary Structure
MKIAVLPGDGIGPEIVAEAVKVLNALDEKFEMETAPVGGAGYEAEGHPLPENTLKLAKEA
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHC
DAILFGAVGDWKYDSLERALRPEQAILGLRKHLQLFANFRPAICYPELTGASSLKPELVA
CEEEEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHH
GLDILIVRELNGDIYFGQPRGVREAPDGLFKGAREGFDTMRYSEPEIRRIAHVAFQAAAK
CCEEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RGKKLCSVDKANVLETFQFWKDIVIDVSKEYPDVELSHMYVDNAAMQLVKAPKSFDVIVT
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEHHEEHHHHHHHHHHCCCCCEEEEE
GNMFGDILSDEAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGVANPLATILSA
CCHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AMMLRYSLNKAEQADRIENAVKKVLAQGYRTGDILTPGCKQVGTREMGEAVLAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]