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The map label for this gene is truA

Identifier: 73541994

GI number: 73541994

Start: 2544782

End: 2545603

Strand: Reverse

Name: truA

Synonym: Reut_A2308

Alternate gene names: 73541994

Gene position: 2545603-2544782 (Counterclockwise)

Preceding gene: 73541995

Following gene: 73541993

Centisome position: 66.87

GC content: 64.72

Gene sequence:

>822_bases
ATGAACCGCATCGCCATCGGCCTCCACTACGACGGTGCCGCCTTTTCGGGCTGGCAGTCGCAGCCGCATCGCAACACCGT
GCAGGATTACCTCGAAGACTCCATCGAGCGCTTCGCGGGCGTGCGCCTGTTGACGACCGTTGCCGGGCGCACTGATGCCG
GCGTCCATGCGCTGGGGCAGGTGATCCATCTCGACACGACGCTGGATCGCACCACGTTCTCATGGGTGCGCGGCATCAAT
GCATTCCTGCCGCCGTCGATCGCGCTGCAATGGGCTCGGCCGGTCGACGAGAGTTTCCATGCGCGTTTCCTGGCGTTTGA
GCGAATGTACTATTACGCGCTCTACACTGGCCCGCACCGCGTGCCACTCGCGCATGGCCGGGCCGGGTACCTTATGTTGC
CGCCGGGCCAGAGGCTTGACGTGGATGCCATGCAGGCCGCGTCGCGCTGCCTGCTTGGCGAGCAGGATTTCTCGTCGTTC
CGTGCGGCCGAGTGCCAGGCCAAGTCACCGGTCAAGACCATGTATGACGTGACGTTGAAGGCGGACGGCAACTGGGTCTT
CCTGCGCTTCCGCGCGAGCGCCTTCCTGCATCACATGGTGCGCAACCTGATGGGTTGCCTGGTGGCGGTAGGGCGCGGAC
GCTATCCGCCGGAATGGCTGGCCGAAGTGCTCGCCGCCCGTGATCGCAAGCTCGCAGCGCCCACCTTCATGCCGGACGGT
CTCTACCTCGCGGACGTGAAGTATCCTGAGGCTTACAAGATTCCGGCGGCGGACCCATCCGCCAGCCTGTTCCACGGAGT
ATTCCGCCATGACGCAGCCTGA

Upstream 100 bases:

>100_bases
GGGCAGGCGGCAGTCATGCCGCGGCCCGAAGTGATGCGCCGGAAGCGTCCCTTCCGGCGCATTTGTTTTTGTCGGCCATC
GCAGGCCAGTTTCCCGCTCC

Downstream 100 bases:

>100_bases
GCGTGCCGACGCCGCGGCACCGGCCCGCACCCGCATCAAGATCTGCGGCCTCACGCGCGAGGACGACGTCCGCGCCGCGG
TTGACGCGGGTGCTGACGCA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MNRIAIGLHYDGAAFSGWQSQPHRNTVQDYLEDSIERFAGVRLLTTVAGRTDAGVHALGQVIHLDTTLDRTTFSWVRGIN
AFLPPSIALQWARPVDESFHARFLAFERMYYYALYTGPHRVPLAHGRAGYLMLPPGQRLDVDAMQAASRCLLGEQDFSSF
RAAECQAKSPVKTMYDVTLKADGNWVFLRFRASAFLHHMVRNLMGCLVAVGRGRYPPEWLAEVLAARDRKLAAPTFMPDG
LYLADVKYPEAYKIPAADPSASLFHGVFRHDAA

Sequences:

>Translated_273_residues
MNRIAIGLHYDGAAFSGWQSQPHRNTVQDYLEDSIERFAGVRLLTTVAGRTDAGVHALGQVIHLDTTLDRTTFSWVRGIN
AFLPPSIALQWARPVDESFHARFLAFERMYYYALYTGPHRVPLAHGRAGYLMLPPGQRLDVDAMQAASRCLLGEQDFSSF
RAAECQAKSPVKTMYDVTLKADGNWVFLRFRASAFLHHMVRNLMGCLVAVGRGRYPPEWLAEVLAARDRKLAAPTFMPDG
LYLADVKYPEAYKIPAADPSASLFHGVFRHDAA
>Mature_273_residues
MNRIAIGLHYDGAAFSGWQSQPHRNTVQDYLEDSIERFAGVRLLTTVAGRTDAGVHALGQVIHLDTTLDRTTFSWVRGIN
AFLPPSIALQWARPVDESFHARFLAFERMYYYALYTGPHRVPLAHGRAGYLMLPPGQRLDVDAMQAASRCLLGEQDFSSF
RAAECQAKSPVKTMYDVTLKADGNWVFLRFRASAFLHHMVRNLMGCLVAVGRGRYPPEWLAEVLAARDRKLAAPTFMPDG
LYLADVKYPEAYKIPAADPSASLFHGVFRHDAA

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=272, Percent_Identity=33.4558823529412, Blast_Score=102, Evalue=5e-22,
Organism=Escherichia coli, GI1788657, Length=262, Percent_Identity=42.3664122137405, Blast_Score=221, Evalue=4e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_CUPPJ (Q46YW3)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_296514.1
- ProteinModelPortal:   Q46YW3
- SMR:   Q46YW3
- GeneID:   3609014
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A2308
- NMPDR:   fig|264198.3.peg.3038
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BioCyc:   REUT264198:REUT_A2308-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 30544; Mature: 30544

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIAIGLHYDGAAFSGWQSQPHRNTVQDYLEDSIERFAGVRLLTTVAGRTDAGVHALGQ
CCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
VIHLDTTLDRTTFSWVRGINAFLPPSIALQWARPVDESFHARFLAFERMYYYALYTGPHR
EEEECCCCCHHHHHHHHCCHHHCCCHHEEEECCCCCHHHHHHHHHHHHHEEEEEEECCCC
VPLAHGRAGYLMLPPGQRLDVDAMQAASRCLLGEQDFSSFRAAECQAKSPVKTMYDVTLK
CEECCCCCCEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEEE
ADGNWVFLRFRASAFLHHMVRNLMGCLVAVGRGRYPPEWLAEVLAARDRKLAAPTFMPDG
ECCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
LYLADVKYPEAYKIPAADPSASLFHGVFRHDAA
EEEEECCCCCCEECCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MNRIAIGLHYDGAAFSGWQSQPHRNTVQDYLEDSIERFAGVRLLTTVAGRTDAGVHALGQ
CCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
VIHLDTTLDRTTFSWVRGINAFLPPSIALQWARPVDESFHARFLAFERMYYYALYTGPHR
EEEECCCCCHHHHHHHHCCHHHCCCHHEEEECCCCCHHHHHHHHHHHHHEEEEEEECCCC
VPLAHGRAGYLMLPPGQRLDVDAMQAASRCLLGEQDFSSFRAAECQAKSPVKTMYDVTLK
CEECCCCCCEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEEE
ADGNWVFLRFRASAFLHHMVRNLMGCLVAVGRGRYPPEWLAEVLAARDRKLAAPTFMPDG
ECCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
LYLADVKYPEAYKIPAADPSASLFHGVFRHDAA
EEEEECCCCCCEECCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA