Click here to switch to the map view.

The map label for this gene is trpB [H]

Identifier: 73541992

GI number: 73541992

Start: 2542763

End: 2543956

Strand: Reverse

Name: trpB [H]

Synonym: Reut_A2306

Alternate gene names: 73541992

Gene position: 2543956-2542763 (Counterclockwise)

Preceding gene: 73541993

Following gene: 73541991

Centisome position: 66.83

GC content: 64.99

Gene sequence:

>1194_bases
ATGTACGACCTGCCCGATTCCCGTGGCCATTTCGGCCCCTATGGCGGCACCTTCGTCTCCGAGACGCTGGTCCACGCACT
CGATGAGCTGCGCGAGGCCTATGCCCATTACCAGCAAGACGCCGAGTTCGACGCCGAATTCCGCCGCGAGCTGAAGCACT
TCGTCGGCCGTCCATCGCCGATCTATCATGCGCAGCGCTGGAGCGAGCTGCTCGGCGGCGCGCAGATCTACCTCAAGCGC
GAGGACCTGAACCACACCGGCGCCCACAAGATCAACAACGTGATCGGCCAGGCGCTGCTGGCCAAGCGCATGGGCAAGAA
GCGCGTGATCGCCGAGACCGGCGCCGGCCAGCACGGCGTTGCCACGGCCACTATCGCCGCACGCTTCGGCATGGAGTGCG
TGGTCTACATGGGCTCCGAGGATGTCAAGCGCCAGGCCGCGAACGTCTATCGCATGAAGCTGCTCGGCGCGACCGTCGTT
CCGGTGGAAAGTGGCTCGCGCACGCTCAAGGATGCGCTCAATGAAGCGATGCGCGACTGGGTGACCAACGTCGAAACCAC
GTTCTACATCATTGGTACCGTGGCCGGCCCGCACCCGTACCCGATGATGGTGCGCGATTTCCAGTGCGTGATCGGCGAGG
AAGCCAAGGTGCAGATGCCCGAGATGACTGGCCGCCAGCCGGATGCCGTGATCGCCTGCGTGGGCGGCGGCTCGAATGCA
ATGGGCATCTTCTATCCGTACATCGAGCACAAGGACGTCCAGCTGCTCGGTGTCGAAGCCGCCGGCGATGGGCTGGACAC
GGGGCGCCATGCAGCGGCACTGATCGGCGGTACGCCGGGCGTGCTGCATGGCAACCGCACCTACCTGCTGCAGGACGAAA
ATGGGCAGATCATCGAGACGCATTCGGTGTCGGCCGGTCTGGACTATCCGGGCGTCGGTCCCGAGCACGCGTGGCTCAAG
GACATCGGCCGCGCGCAGTATGTGCCGATCACCGATGAGGAGGCGCTCAAGGCCTTCCACGATTGCTGCCGCATCGAGGG
CATCATCCCCGCGCTGGAATCGAGCCATGCGATCGCCTACGCGTGCAAGCTCGCGCCGACACTGCCCAAGGACAAGCTGC
TGCTGGTGAACCTGTCCGGCCGCGGCGACAAGGACATGCACACCGTCGCCGAACGCTCGGGGCTCAAGCTCTGA

Upstream 100 bases:

>100_bases
TCACCATGGCCCCGGCGAATTGCAGTAGCGCCGCTCGCATCGCAGTCAAAATTTGCGCCCTTGCCCGTGAGGCAAGGCCG
TTGACCTGAAGAGCCCAGAC

Downstream 100 bases:

>100_bases
TGCGCGCGCTGCCACCTTCCGTACCGTCGGAGCTGGCCCTGGGCGGGGCTTCGGACCTGCCCGGCGCGCCGGACGCGCCT
GCGCTGCAGTTGTTCCAGGA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MYDLPDSRGHFGPYGGTFVSETLVHALDELREAYAHYQQDAEFDAEFRRELKHFVGRPSPIYHAQRWSELLGGAQIYLKR
EDLNHTGAHKINNVIGQALLAKRMGKKRVIAETGAGQHGVATATIAARFGMECVVYMGSEDVKRQAANVYRMKLLGATVV
PVESGSRTLKDALNEAMRDWVTNVETTFYIIGTVAGPHPYPMMVRDFQCVIGEEAKVQMPEMTGRQPDAVIACVGGGSNA
MGIFYPYIEHKDVQLLGVEAAGDGLDTGRHAAALIGGTPGVLHGNRTYLLQDENGQIIETHSVSAGLDYPGVGPEHAWLK
DIGRAQYVPITDEEALKAFHDCCRIEGIIPALESSHAIAYACKLAPTLPKDKLLLVNLSGRGDKDMHTVAERSGLKL

Sequences:

>Translated_397_residues
MYDLPDSRGHFGPYGGTFVSETLVHALDELREAYAHYQQDAEFDAEFRRELKHFVGRPSPIYHAQRWSELLGGAQIYLKR
EDLNHTGAHKINNVIGQALLAKRMGKKRVIAETGAGQHGVATATIAARFGMECVVYMGSEDVKRQAANVYRMKLLGATVV
PVESGSRTLKDALNEAMRDWVTNVETTFYIIGTVAGPHPYPMMVRDFQCVIGEEAKVQMPEMTGRQPDAVIACVGGGSNA
MGIFYPYIEHKDVQLLGVEAAGDGLDTGRHAAALIGGTPGVLHGNRTYLLQDENGQIIETHSVSAGLDYPGVGPEHAWLK
DIGRAQYVPITDEEALKAFHDCCRIEGIIPALESSHAIAYACKLAPTLPKDKLLLVNLSGRGDKDMHTVAERSGLKL
>Mature_397_residues
MYDLPDSRGHFGPYGGTFVSETLVHALDELREAYAHYQQDAEFDAEFRRELKHFVGRPSPIYHAQRWSELLGGAQIYLKR
EDLNHTGAHKINNVIGQALLAKRMGKKRVIAETGAGQHGVATATIAARFGMECVVYMGSEDVKRQAANVYRMKLLGATVV
PVESGSRTLKDALNEAMRDWVTNVETTFYIIGTVAGPHPYPMMVRDFQCVIGEEAKVQMPEMTGRQPDAVIACVGGGSNA
MGIFYPYIEHKDVQLLGVEAAGDGLDTGRHAAALIGGTPGVLHGNRTYLLQDENGQIIETHSVSAGLDYPGVGPEHAWLK
DIGRAQYVPITDEEALKAFHDCCRIEGIIPALESSHAIAYACKLAPTLPKDKLLLVNLSGRGDKDMHTVAERSGLKL

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=381, Percent_Identity=56.6929133858268, Blast_Score=438, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6321412, Length=382, Percent_Identity=57.8534031413613, Blast_Score=462, Evalue=1e-131,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 43350; Mature: 43350

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDLPDSRGHFGPYGGTFVSETLVHALDELREAYAHYQQDAEFDAEFRRELKHFVGRPSP
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCC
IYHAQRWSELLGGAQIYLKREDLNHTGAHKINNVIGQALLAKRMGKKRVIAETGAGQHGV
CHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
ATATIAARFGMECVVYMGSEDVKRQAANVYRMKLLGATVVPVESGSRTLKDALNEAMRDW
HHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHH
VTNVETTFYIIGTVAGPHPYPMMVRDFQCVIGEEAKVQMPEMTGRQPDAVIACVGGGSNA
HCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEEECCCCCC
MGIFYPYIEHKDVQLLGVEAAGDGLDTGRHAAALIGGTPGVLHGNRTYLLQDENGQIIET
CEEEEECCCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCEEECCEEEEEECCCCCEEEE
HSVSAGLDYPGVGPEHAWLKDIGRAQYVPITDEEALKAFHDCCRIEGIIPALESSHAIAY
CCCCCCCCCCCCCCCHHHHHHCCCCEEECCCCHHHHHHHHHHHHHCCCCHHHCCCCEEEE
ACKLAPTLPKDKLLLVNLSGRGDKDMHTVAERSGLKL
EEECCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MYDLPDSRGHFGPYGGTFVSETLVHALDELREAYAHYQQDAEFDAEFRRELKHFVGRPSP
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCC
IYHAQRWSELLGGAQIYLKREDLNHTGAHKINNVIGQALLAKRMGKKRVIAETGAGQHGV
CHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
ATATIAARFGMECVVYMGSEDVKRQAANVYRMKLLGATVVPVESGSRTLKDALNEAMRDW
HHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHH
VTNVETTFYIIGTVAGPHPYPMMVRDFQCVIGEEAKVQMPEMTGRQPDAVIACVGGGSNA
HCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEEECCCCCC
MGIFYPYIEHKDVQLLGVEAAGDGLDTGRHAAALIGGTPGVLHGNRTYLLQDENGQIIET
CEEEEECCCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCEEECCEEEEEECCCCCEEEE
HSVSAGLDYPGVGPEHAWLKDIGRAQYVPITDEEALKAFHDCCRIEGIIPALESSHAIAY
CCCCCCCCCCCCCCCHHHHHHCCCCEEECCCCHHHHHHHHHHHHHCCCCHHHCCCCEEEE
ACKLAPTLPKDKLLLVNLSGRGDKDMHTVAERSGLKL
EEECCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]