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The map label for this gene is hpaIM [H]

Identifier: 73541991

GI number: 73541991

Start: 2541849

End: 2542763

Strand: Reverse

Name: hpaIM [H]

Synonym: Reut_A2305

Alternate gene names: 73541991

Gene position: 2542763-2541849 (Counterclockwise)

Preceding gene: 73541992

Following gene: 73541990

Centisome position: 66.8

GC content: 66.99

Gene sequence:

>915_bases
ATGCGCGCGCTGCCACCTTCCGTACCGTCGGAGCTGGCCCTGGGCGGGGCTTCGGACCTGCCCGGCGCGCCGGACGCGCC
TGCGCTGCAGTTGTTCCAGGAAGACATGTTCGAAGGCATTGCACGCCTGCCGGACGGCTCGGTCGACCTCGTCGTCGCCG
ATCCGCCCTACGGGCTGGGCAAGGACTACGGCAATGACTCCGACTTGCTGTCGGGCGATGCCTACCTCGAGTGGTCCGAG
CGCTGGATGGATGCCGTCTGCCCGAAGCTGGCGCCCAAAGGCACGCTGTACCTGTTCTGCACCTGGCAGTATTCGCCCGA
GCTGTTCGTGATGCTCAAGCGCCGCCTGACGATGATCAACGAGATCATCTGGGACCGTCGCGTGCCGAGCATGGGCGGCA
CCACGCGCAAGTATTCGTCGGTGCACGACAACATCGGCTTCTTCGCCAGGGCGCGCGATTACTACTTCGACCTGGACCCG
GTGCGAATTCCGTACGACCCGGAGACCAAGAAGGCGCGCAGCCGCCCGCGTTTCGAGGGCAAGAAGTGGCTCGAGATGGG
CTACAACCCGAAGGACCTGTGGAGCATCTCGCGCATCCACCGCCAGGATCCCGAGCGCGCCAATCACCCGACGCAGAAGC
CGCTGGAAATCGTCGAGCGCATGGTGCTGTCGAGCTGCCCGCCGGGCGGGCTGGTGCTGGACCCGTTCGCGGGCAGCGGC
ACTACCGCGGTGGCGTGCCTGCGCCATGGCCGGCGCTTCGCGGGCTTCGAGATCAATCCCGAGTATGTCGAGGTGGCGCG
CGGGCGTGTGGCCGCGGCCGCGCAACTGGCCAGGTCCGAGGCGGACCGCAACCCAACATCGGCGGCTGCCAATGACGAGG
ATTCGTCGGCGCAGCTGCCTCACCTGATTCCCTGA

Upstream 100 bases:

>100_bases
CGCCGACACTGCCCAAGGACAAGCTGCTGCTGGTGAACCTGTCCGGCCGCGGCGACAAGGACATGCACACCGTCGCCGAA
CGCTCGGGGCTCAAGCTCTG

Downstream 100 bases:

>100_bases
TATGTCACGCATCCAGAAGACATTCGCGGCACTCGCCGCGCAGAACAAGAAGGGCCTGATTCCGTTCATTACCGCAGGCG
ACCCGGAACCGGGGCTGACG

Product: DNA methylase N-4/N-6

Products: NA

Alternate protein names: M.HpaI; Adenine-specific methyltransferase HpaI [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MRALPPSVPSELALGGASDLPGAPDAPALQLFQEDMFEGIARLPDGSVDLVVADPPYGLGKDYGNDSDLLSGDAYLEWSE
RWMDAVCPKLAPKGTLYLFCTWQYSPELFVMLKRRLTMINEIIWDRRVPSMGGTTRKYSSVHDNIGFFARARDYYFDLDP
VRIPYDPETKKARSRPRFEGKKWLEMGYNPKDLWSISRIHRQDPERANHPTQKPLEIVERMVLSSCPPGGLVLDPFAGSG
TTAVACLRHGRRFAGFEINPEYVEVARGRVAAAAQLARSEADRNPTSAAANDEDSSAQLPHLIP

Sequences:

>Translated_304_residues
MRALPPSVPSELALGGASDLPGAPDAPALQLFQEDMFEGIARLPDGSVDLVVADPPYGLGKDYGNDSDLLSGDAYLEWSE
RWMDAVCPKLAPKGTLYLFCTWQYSPELFVMLKRRLTMINEIIWDRRVPSMGGTTRKYSSVHDNIGFFARARDYYFDLDP
VRIPYDPETKKARSRPRFEGKKWLEMGYNPKDLWSISRIHRQDPERANHPTQKPLEIVERMVLSSCPPGGLVLDPFAGSG
TTAVACLRHGRRFAGFEINPEYVEVARGRVAAAAQLARSEADRNPTSAAANDEDSSAQLPHLIP
>Mature_304_residues
MRALPPSVPSELALGGASDLPGAPDAPALQLFQEDMFEGIARLPDGSVDLVVADPPYGLGKDYGNDSDLLSGDAYLEWSE
RWMDAVCPKLAPKGTLYLFCTWQYSPELFVMLKRRLTMINEIIWDRRVPSMGGTTRKYSSVHDNIGFFARARDYYFDLDP
VRIPYDPETKKARSRPRFEGKKWLEMGYNPKDLWSISRIHRQDPERANHPTQKPLEIVERMVLSSCPPGGLVLDPFAGSG
TTAVACLRHGRRFAGFEINPEYVEVARGRVAAAAQLARSEADRNPTSAAANDEDSSAQLPHLIP

Specific function: This methylase recognizes the double-stranded sequence GTTAAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the HpaI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=270, Percent_Identity=28.8888888888889, Blast_Score=129, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 33852; Mature: 33852

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALPPSVPSELALGGASDLPGAPDAPALQLFQEDMFEGIARLPDGSVDLVVADPPYGLG
CCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
KDYGNDSDLLSGDAYLEWSERWMDAVCPKLAPKGTLYLFCTWQYSPELFVMLKRRLTMIN
CCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHH
EIIWDRRVPSMGGTTRKYSSVHDNIGFFARARDYYFDLDPVRIPYDPETKKARSRPRFEG
HHHHHCCCCCCCCCCHHHHHHHHCCHHEEECCCCEECCCCEECCCCCCHHHHHCCCCCCC
KKWLEMGYNPKDLWSISRIHRQDPERANHPTQKPLEIVERMVLSSCPPGGLVLDPFAGSG
HHHHHCCCCHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCC
TTAVACLRHGRRFAGFEINPEYVEVARGRVAAAAQLARSEADRNPTSAAANDEDSSAQLP
HHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
HLIP
CCCC
>Mature Secondary Structure
MRALPPSVPSELALGGASDLPGAPDAPALQLFQEDMFEGIARLPDGSVDLVVADPPYGLG
CCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
KDYGNDSDLLSGDAYLEWSERWMDAVCPKLAPKGTLYLFCTWQYSPELFVMLKRRLTMIN
CCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHH
EIIWDRRVPSMGGTTRKYSSVHDNIGFFARARDYYFDLDPVRIPYDPETKKARSRPRFEG
HHHHHCCCCCCCCCCHHHHHHHHCCHHEEECCCCEECCCCEECCCCCCHHHHHCCCCCCC
KKWLEMGYNPKDLWSISRIHRQDPERANHPTQKPLEIVERMVLSSCPPGGLVLDPFAGSG
HHHHHCCCCHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCC
TTAVACLRHGRRFAGFEINPEYVEVARGRVAAAAQLARSEADRNPTSAAANDEDSSAQLP
HHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
HLIP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1542567 [H]