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The map label for this gene is trpA

Identifier: 73541990

GI number: 73541990

Start: 2541050

End: 2541847

Strand: Reverse

Name: trpA

Synonym: Reut_A2304

Alternate gene names: 73541990

Gene position: 2541847-2541050 (Counterclockwise)

Preceding gene: 73541991

Following gene: 73541989

Centisome position: 66.78

GC content: 66.04

Gene sequence:

>798_bases
ATGTCACGCATCCAGAAGACATTCGCGGCACTCGCCGCGCAGAACAAGAAGGGCCTGATTCCGTTCATTACCGCAGGCGA
CCCGGAACCGGGGCTGACGGTGGACCTGATGCACGCACTCGTTGCCGGCGGCGCCGACGTGATCGAGCTCGGCGTGCCGT
TCTCCGATCCGATGGCCGATGGCCCGGTGATCCAGCGCGCCTCCGAGCGCGCGCTCGCCAATGGCGTGTCGCTCACGCAG
GTGCTGCAGTGGGTGCGTGAATTCCGCCAGACCAATGCCGATACGCCGGTCGTGCTGATGGGCTATGCCAACCCGATCGA
GCGCATGGGCGAGGCCGCATTTGCCAAGGCCGCTGGTGCTGCTGGCGTGGACGGCGTGCTCGTGGTCGACTATCCGCCGG
AAGAGTGCGAGTCGTTCGCCGTGCTGATGCGCGACAACGGCATCGATCCGATCTTCCTGCTGGCGCCGACTTCGACGGAT
GCGCGCATCGAGGCCGTTGGCAAGGTGGCGAGCGGCTATGTCTACTACGTCTCGCTCAAGGGCGTGACCGGTTCGGCCAC
GCTGGATCTCGACAGTGTCGCGGCACGCCTCCCACTGATCAAGAAGCACGTGAACCTGCCTGTTGGCGTGGGCTTTGGCA
TCCGTGATGCGCAGACGGCACGCGCGATCGGCAGCGTGGCGGACGCCGTGGTGATCGGTAGCCGGCTGGTGCAGCTGCTC
GAAGATGCGCCGCGCGGACAGGCGGTAGAATCGCTGCGTGCGTTCATCGCCGGAATTCGCGAGGCGCTGGACGCCTGA

Upstream 100 bases:

>100_bases
GCCGCGCAACTGGCCAGGTCCGAGGCGGACCGCAACCCAACATCGGCGGCTGCCAATGACGAGGATTCGTCGGCGCAGCT
GCCTCACCTGATTCCCTGAT

Downstream 100 bases:

>100_bases
AGTGATGAGTTGGCTGCCTGATGGTGGCGAAAAAGTGCCGGAGCCTGCGCGCCATGCGTGTTCTGCTTCGGCCGAACCAT
GCCGGCATGGTAAATTCTCG

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MSRIQKTFAALAAQNKKGLIPFITAGDPEPGLTVDLMHALVAGGADVIELGVPFSDPMADGPVIQRASERALANGVSLTQ
VLQWVREFRQTNADTPVVLMGYANPIERMGEAAFAKAAGAAGVDGVLVVDYPPEECESFAVLMRDNGIDPIFLLAPTSTD
ARIEAVGKVASGYVYYVSLKGVTGSATLDLDSVAARLPLIKKHVNLPVGVGFGIRDAQTARAIGSVADAVVIGSRLVQLL
EDAPRGQAVESLRAFIAGIREALDA

Sequences:

>Translated_265_residues
MSRIQKTFAALAAQNKKGLIPFITAGDPEPGLTVDLMHALVAGGADVIELGVPFSDPMADGPVIQRASERALANGVSLTQ
VLQWVREFRQTNADTPVVLMGYANPIERMGEAAFAKAAGAAGVDGVLVVDYPPEECESFAVLMRDNGIDPIFLLAPTSTD
ARIEAVGKVASGYVYYVSLKGVTGSATLDLDSVAARLPLIKKHVNLPVGVGFGIRDAQTARAIGSVADAVVIGSRLVQLL
EDAPRGQAVESLRAFIAGIREALDA
>Mature_264_residues
SRIQKTFAALAAQNKKGLIPFITAGDPEPGLTVDLMHALVAGGADVIELGVPFSDPMADGPVIQRASERALANGVSLTQV
LQWVREFRQTNADTPVVLMGYANPIERMGEAAFAKAAGAAGVDGVLVVDYPPEECESFAVLMRDNGIDPIFLLAPTSTDA
RIEAVGKVASGYVYYVSLKGVTGSATLDLDSVAARLPLIKKHVNLPVGVGFGIRDAQTARAIGSVADAVVIGSRLVQLLE
DAPRGQAVESLRAFIAGIREALDA

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=269, Percent_Identity=34.2007434944238, Blast_Score=140, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6321412, Length=260, Percent_Identity=41.9230769230769, Blast_Score=209, Evalue=5e-55,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_CUPPJ (Q46YW7)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_296510.1
- HSSP:   P42390
- ProteinModelPortal:   Q46YW7
- SMR:   Q46YW7
- GeneID:   3609010
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A2304
- NMPDR:   fig|264198.3.peg.3034
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- BioCyc:   REUT264198:REUT_A2304-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 27722; Mature: 27591

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIQKTFAALAAQNKKGLIPFITAGDPEPGLTVDLMHALVAGGADVIELGVPFSDPMAD
CCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPVIQRASERALANGVSLTQVLQWVREFRQTNADTPVVLMGYANPIERMGEAAFAKAAGA
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCC
AGVDGVLVVDYPPEECESFAVLMRDNGIDPIFLLAPTSTDARIEAVGKVASGYVYYVSLK
CCCCCEEEEECCHHHHHHHEEHEECCCCCEEEEEECCCCCHHHHHHHHHHCCEEEEEEEE
GVTGSATLDLDSVAARLPLIKKHVNLPVGVGFGIRDAQTARAIGSVADAVVIGSRLVQLL
CCCCCCEECHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EDAPRGQAVESLRAFIAGIREALDA
HCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRIQKTFAALAAQNKKGLIPFITAGDPEPGLTVDLMHALVAGGADVIELGVPFSDPMAD
CHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPVIQRASERALANGVSLTQVLQWVREFRQTNADTPVVLMGYANPIERMGEAAFAKAAGA
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCC
AGVDGVLVVDYPPEECESFAVLMRDNGIDPIFLLAPTSTDARIEAVGKVASGYVYYVSLK
CCCCCEEEEECCHHHHHHHEEHEECCCCCEEEEEECCCCCHHHHHHHHHHCCEEEEEEEE
GVTGSATLDLDSVAARLPLIKKHVNLPVGVGFGIRDAQTARAIGSVADAVVIGSRLVQLL
CCCCCCEECHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EDAPRGQAVESLRAFIAGIREALDA
HCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA