Click here to switch to the map view.

The map label for this gene is purU [H]

Identifier: 73541898

GI number: 73541898

Start: 2444220

End: 2445086

Strand: Reverse

Name: purU [H]

Synonym: Reut_A2211

Alternate gene names: 73541898

Gene position: 2445086-2444220 (Counterclockwise)

Preceding gene: 73541899

Following gene: 73541896

Centisome position: 64.23

GC content: 63.32

Gene sequence:

>867_bases
ATGAGCAACACCGGATTCATTCTTACTCTTTCGTGCCCGGACCAGCCGGGCATCGTTCATTCCGTCTCCGGCCTGCTGTT
CCAGCACGGCTGCAATATCGTCGATTCCGACCAGTATGGTGATGAATTCACCGGCCGCTTCTTCATGCGGGTGCATTTCA
CCCCGTCGGCCGGCGGTCCGGACCTGGATGCGCTGAAGGCGGCGTTCGCGCCGGTCGGCGACCAGTTCGGCATGCAGTGG
GAGCTGTTCGATGCCTCCGCCAAGCCGCGCGTGATGATCATGGTGTCCAAGATCGGACACTGCCTGAACGACCTGCTGTT
CCGTGCCAAGGTGGGCGGCCTGCCGGTCGAGATTGCCGCCATCGTCTCGAACCACCGCGACTTCTACCAGCTCGCCGCGT
CCTACGACGTGCCGTTCATCCACCTGCCGCTGATGAACGCGTCGGCCGAGCAGAAGGCCGCGCAGGAAGCGCGCGTGTTT
GAAGTGGTGCGCGACCAGAACATCGACCTCGTCGTGCTCGCGCGCTACATGCAGGTGCTGTCGGATGACCTGTGCCGCAA
GCTGGCCGGCCGCGCGATCAATATCCACCACTCGTTCCTGCCGAGCTTCAAGGGTGCCAAGCCGTATTACCAGGCCCATG
ACCGCGGCGTGAAGCTGATCGGGGCGACCGCGCACTATGTGACGGCCGATCTCGACGAAGGCCCGATCATCGAGCAGGAA
ATCGCGCGCGTGGATCACAGCATGGACCCCGACCAGCTCACGTCCGTGGGCCGCGATGTCGAATGCGTGGCGCTCGCCCG
CGCAGTCAAGTGGCATGCGGAACACCGCATCCTGCTGAACGGCCACAAGACCGTCGTCTTCAAGTAA

Upstream 100 bases:

>100_bases
CTGGCTGGCCCCGGCGGGCAATCCCGCCTGATGCAGGGCCCCGGGCCCCGCCTGCGCTCTCCCGGCCCCGTGCCGCACGC
ACCCGACAGAACAACACACG

Downstream 100 bases:

>100_bases
TTGCTGCAATTCGCCGCCGGCGCGCATCGCGCCGGCCGGTATGCGGATACTGGCGTGCGCCGCCGCGGCGGCAGGGTCAG
TCCTGGTCCGCCACCACACA

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MSNTGFILTLSCPDQPGIVHSVSGLLFQHGCNIVDSDQYGDEFTGRFFMRVHFTPSAGGPDLDALKAAFAPVGDQFGMQW
ELFDASAKPRVMIMVSKIGHCLNDLLFRAKVGGLPVEIAAIVSNHRDFYQLAASYDVPFIHLPLMNASAEQKAAQEARVF
EVVRDQNIDLVVLARYMQVLSDDLCRKLAGRAINIHHSFLPSFKGAKPYYQAHDRGVKLIGATAHYVTADLDEGPIIEQE
IARVDHSMDPDQLTSVGRDVECVALARAVKWHAEHRILLNGHKTVVFK

Sequences:

>Translated_288_residues
MSNTGFILTLSCPDQPGIVHSVSGLLFQHGCNIVDSDQYGDEFTGRFFMRVHFTPSAGGPDLDALKAAFAPVGDQFGMQW
ELFDASAKPRVMIMVSKIGHCLNDLLFRAKVGGLPVEIAAIVSNHRDFYQLAASYDVPFIHLPLMNASAEQKAAQEARVF
EVVRDQNIDLVVLARYMQVLSDDLCRKLAGRAINIHHSFLPSFKGAKPYYQAHDRGVKLIGATAHYVTADLDEGPIIEQE
IARVDHSMDPDQLTSVGRDVECVALARAVKWHAEHRILLNGHKTVVFK
>Mature_287_residues
SNTGFILTLSCPDQPGIVHSVSGLLFQHGCNIVDSDQYGDEFTGRFFMRVHFTPSAGGPDLDALKAAFAPVGDQFGMQWE
LFDASAKPRVMIMVSKIGHCLNDLLFRAKVGGLPVEIAAIVSNHRDFYQLAASYDVPFIHLPLMNASAEQKAAQEARVFE
VVRDQNIDLVVLARYMQVLSDDLCRKLAGRAINIHHSFLPSFKGAKPYYQAHDRGVKLIGATAHYVTADLDEGPIIEQEI
ARVDHSMDPDQLTSVGRDVECVALARAVKWHAEHRILLNGHKTVVFK

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=153, Percent_Identity=30.718954248366, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI209869995, Length=153, Percent_Identity=30.718954248366, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI209869993, Length=153, Percent_Identity=30.718954248366, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1787483, Length=285, Percent_Identity=38.9473684210526, Blast_Score=214, Evalue=6e-57,
Organism=Escherichia coli, GI1788846, Length=190, Percent_Identity=25.7894736842105, Blast_Score=75, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17567511, Length=174, Percent_Identity=31.6091954022989, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24582400, Length=196, Percent_Identity=28.0612244897959, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 31853; Mature: 31722

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTGFILTLSCPDQPGIVHSVSGLLFQHGCNIVDSDQYGDEFTGRFFMRVHFTPSAGGP
CCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEEECCCCCCC
DLDALKAAFAPVGDQFGMQWELFDASAKPRVMIMVSKIGHCLNDLLFRAKVGGLPVEIAA
CHHHHHHHHCCCCHHHCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEHH
IVSNHRDFYQLAASYDVPFIHLPLMNASAEQKAAQEARVFEVVRDQNIDLVVLARYMQVL
HHHCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
SDDLCRKLAGRAINIHHSFLPSFKGAKPYYQAHDRGVKLIGATAHYVTADLDEGPIIEQE
HHHHHHHHHCCEEEEEHHHCCCCCCCCCHHHHCCCCEEEEEEEEEEEEEECCCCCCHHHH
IARVDHSMDPDQLTSVGRDVECVALARAVKWHAEHRILLNGHKTVVFK
HHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCEEEEEC
>Mature Secondary Structure 
SNTGFILTLSCPDQPGIVHSVSGLLFQHGCNIVDSDQYGDEFTGRFFMRVHFTPSAGGP
CCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEEECCCCCCC
DLDALKAAFAPVGDQFGMQWELFDASAKPRVMIMVSKIGHCLNDLLFRAKVGGLPVEIAA
CHHHHHHHHCCCCHHHCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEHH
IVSNHRDFYQLAASYDVPFIHLPLMNASAEQKAAQEARVFEVVRDQNIDLVVLARYMQVL
HHHCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
SDDLCRKLAGRAINIHHSFLPSFKGAKPYYQAHDRGVKLIGATAHYVTADLDEGPIIEQE
HHHHHHHHHCCEEEEEHHHCCCCCCCCCHHHHCCCCEEEEEEEEEEEEEECCCCCCHHHH
IARVDHSMDPDQLTSVGRDVECVALARAVKWHAEHRILLNGHKTVVFK
HHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]