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The map label for this gene is alkK [H]

Identifier: 73541834

GI number: 73541834

Start: 2367058

End: 2368704

Strand: Reverse

Name: alkK [H]

Synonym: Reut_A2146

Alternate gene names: 73541834

Gene position: 2368704-2367058 (Counterclockwise)

Preceding gene: 73541835

Following gene: 73541830

Centisome position: 62.23

GC content: 62.6

Gene sequence:

>1647_bases
ATGCAGCAAAACCTGATCGTATCGACGCCCTCGGCGTACTCGTATCCGCTGCTGGTCAAGCAGCTGCTGATTCACTCGAT
GAGCCTCTATGGCGACCAGGAAATCAGCTACCGCGGCGAAATGCGCTACACGTACCGCGATTTCCGTCGTCGCGTCGGCC
AGCTTGCCTCGACGCTCGCATCGCTCGGCGCCAAGCATGGTTCCACGGTGGCCGTGATGGACTGGGACAGCCATCGCTAT
CTCGAGTGCTACTTCGGCGTGCCGATGATGGGCGCGACGCTGTTCACCGTGAACGTGCGCCTGTCGCCGCAGCAGATCCT
GTACACGCTCAACGATGCTGACGCGGACATCGTGCTGGTCCATCCGGATTTCGTTCCGGTGCTCGACCAGATCCGCAACG
AGCTCACCTGCAATCCGCGCTTCGTGCTGCTGGCCGACGGCCACGCTGCGCCGCAGACGGAACTGCAGTTCGCGGGCGAG
TATGAAGCGCTGCTGGCAGATGCCTCGCCGGATTTCGACTTTCCCGAGTTCGACGAGAACACCAAAGCGGCAACGTTCTA
CACCACCGGTACCACCGGCGACCCGAAGGGCGTTTGCTACAGTCATCGCGACATCGTGCTGCACACGTTGTCCACGTCGG
CCAGCCTCTGCTCGCCGCGCGACGGGCAGCGCATGCATCGCGAAGACGTGTACATGCCGATCACGCCGATGTTCCACGTG
CTGGCCTGGGGCATACCGTACCTGGCCGTCACGCTGGGTTTGCGCACCGTGCTGCCTGGCCGCTACGCGCCGGACGTGCT
GCTGAAGCTGCGTGAAGCGGAAAAGGTCACGTTCTCGCACTGCGTGCCGACTATCCTGCAGATGCTGCTGCAGTCCTCGC
AGGACAGTGGGCAAGATCTCTCCGGCTGGAAGATCATCATCGGCGGCTCCGCACTGCCGCCGTCACTGTGCGAAGCAGCG
CTTGATCGCGGCATCGACATCTTCGCCGGATATGGCATGTCCGAGACCGGCCCCATCGTCTCGCTGGCACAGCTGCCGCC
CAATGTGAAGCCGGCAAATCGCGCCGACGAAGTTCGCATGCGCTGCTCCACGGGCCGACCGGTGGTGATGGCGGACTTCC
GCATCGTCAGCGAAGACATGCAGGACCTGCCGCGCGACGGCAAGAACCGTGGCGAGATCGTGCTGCGTGCGCCTTACCTG
ACCCAGTCGTACTTCAAGAAGCCTGGCGCTTCGGAAGAGTTGTGGGCGGGCGGCTATCTGCATACGCAGGACGTCGCGGT
CATGGGCGAAGACGGCTTCGTGCAGATCGTCGATCGCATCAAGGACGTCATCAAGACCGGTGGCGAATGGGTGTCGTCGA
TCGAAGTCGAGAATCTCATTTCCGAAGTGCCGGGCGTTCAGGAATGCGCGGTGATCGGCGTACCGGATGACAAGTGGGGC
GAGCGGCCGATGGCCTTCGTCGTCCGCAAGGCCGGCGCGGAAGTCGGTGCGGACAGCATCCGCGAAAAGCTGCTGGGACA
TGTCGATTCGCGTCGCATCAGCAAATACGCGGTGCCCGAAGCGGAACGTATCGCCTTCGTGGCGGAGATACCGAAGACCA
GTGTCGGGAAGATCAATAAGAAGCAGCTGCGGGAAAAGGCAGCCTGA

Upstream 100 bases:

>100_bases
CACTACGCCGTGATGGATTACCCGCCTTGCCACCGCAAGATGGAGCGTGCCGGCATCATCGGCAGCAAGCCGTACTGACC
GAACGCCAGAGGAAAACAAT

Downstream 100 bases:

>100_bases
AGGCAAAGGTAGGAAGGGATTGTGCGCTGGCTCTTTGAAGGGGGAGTCAGCGCTTTTTTTTGGTTTGGATTTAGAGGTCT
GGGGTTTTTGAGGGGATGTC

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MQQNLIVSTPSAYSYPLLVKQLLIHSMSLYGDQEISYRGEMRYTYRDFRRRVGQLASTLASLGAKHGSTVAVMDWDSHRY
LECYFGVPMMGATLFTVNVRLSPQQILYTLNDADADIVLVHPDFVPVLDQIRNELTCNPRFVLLADGHAAPQTELQFAGE
YEALLADASPDFDFPEFDENTKAATFYTTGTTGDPKGVCYSHRDIVLHTLSTSASLCSPRDGQRMHREDVYMPITPMFHV
LAWGIPYLAVTLGLRTVLPGRYAPDVLLKLREAEKVTFSHCVPTILQMLLQSSQDSGQDLSGWKIIIGGSALPPSLCEAA
LDRGIDIFAGYGMSETGPIVSLAQLPPNVKPANRADEVRMRCSTGRPVVMADFRIVSEDMQDLPRDGKNRGEIVLRAPYL
TQSYFKKPGASEELWAGGYLHTQDVAVMGEDGFVQIVDRIKDVIKTGGEWVSSIEVENLISEVPGVQECAVIGVPDDKWG
ERPMAFVVRKAGAEVGADSIREKLLGHVDSRRISKYAVPEAERIAFVAEIPKTSVGKINKKQLREKAA

Sequences:

>Translated_548_residues
MQQNLIVSTPSAYSYPLLVKQLLIHSMSLYGDQEISYRGEMRYTYRDFRRRVGQLASTLASLGAKHGSTVAVMDWDSHRY
LECYFGVPMMGATLFTVNVRLSPQQILYTLNDADADIVLVHPDFVPVLDQIRNELTCNPRFVLLADGHAAPQTELQFAGE
YEALLADASPDFDFPEFDENTKAATFYTTGTTGDPKGVCYSHRDIVLHTLSTSASLCSPRDGQRMHREDVYMPITPMFHV
LAWGIPYLAVTLGLRTVLPGRYAPDVLLKLREAEKVTFSHCVPTILQMLLQSSQDSGQDLSGWKIIIGGSALPPSLCEAA
LDRGIDIFAGYGMSETGPIVSLAQLPPNVKPANRADEVRMRCSTGRPVVMADFRIVSEDMQDLPRDGKNRGEIVLRAPYL
TQSYFKKPGASEELWAGGYLHTQDVAVMGEDGFVQIVDRIKDVIKTGGEWVSSIEVENLISEVPGVQECAVIGVPDDKWG
ERPMAFVVRKAGAEVGADSIREKLLGHVDSRRISKYAVPEAERIAFVAEIPKTSVGKINKKQLREKAA
>Mature_548_residues
MQQNLIVSTPSAYSYPLLVKQLLIHSMSLYGDQEISYRGEMRYTYRDFRRRVGQLASTLASLGAKHGSTVAVMDWDSHRY
LECYFGVPMMGATLFTVNVRLSPQQILYTLNDADADIVLVHPDFVPVLDQIRNELTCNPRFVLLADGHAAPQTELQFAGE
YEALLADASPDFDFPEFDENTKAATFYTTGTTGDPKGVCYSHRDIVLHTLSTSASLCSPRDGQRMHREDVYMPITPMFHV
LAWGIPYLAVTLGLRTVLPGRYAPDVLLKLREAEKVTFSHCVPTILQMLLQSSQDSGQDLSGWKIIIGGSALPPSLCEAA
LDRGIDIFAGYGMSETGPIVSLAQLPPNVKPANRADEVRMRCSTGRPVVMADFRIVSEDMQDLPRDGKNRGEIVLRAPYL
TQSYFKKPGASEELWAGGYLHTQDVAVMGEDGFVQIVDRIKDVIKTGGEWVSSIEVENLISEVPGVQECAVIGVPDDKWG
ERPMAFVVRKAGAEVGADSIREKLLGHVDSRRISKYAVPEAERIAFVAEIPKTSVGKINKKQLREKAA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=541, Percent_Identity=26.6173752310536, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI187761345, Length=556, Percent_Identity=26.2589928057554, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI187761343, Length=556, Percent_Identity=26.2589928057554, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI42544132, Length=518, Percent_Identity=24.5173745173745, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI58082049, Length=525, Percent_Identity=24.952380952381, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI157311624, Length=525, Percent_Identity=25.5238095238095, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI157311622, Length=525, Percent_Identity=25.5238095238095, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI38505220, Length=464, Percent_Identity=26.2931034482759, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI122937307, Length=525, Percent_Identity=24.7619047619048, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI115511026, Length=519, Percent_Identity=24.0847784200385, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI28416953, Length=547, Percent_Identity=24.1316270566728, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI13375727, Length=192, Percent_Identity=28.125, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1788107, Length=552, Percent_Identity=27.536231884058, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI221142682, Length=513, Percent_Identity=25.9259259259259, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI145693145, Length=370, Percent_Identity=28.6486486486486, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1786810, Length=523, Percent_Identity=24.4741873804971, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788595, Length=449, Percent_Identity=23.6080178173719, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1790505, Length=536, Percent_Identity=22.0149253731343, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786529, Length=548, Percent_Identity=24.0875912408759, Blast_Score=73, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17559526, Length=515, Percent_Identity=26.9902912621359, Blast_Score=149, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17560308, Length=535, Percent_Identity=24.4859813084112, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17531443, Length=511, Percent_Identity=22.8962818003914, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17560140, Length=520, Percent_Identity=21.5384615384615, Blast_Score=122, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17557194, Length=549, Percent_Identity=24.0437158469945, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71996755, Length=543, Percent_Identity=25.0460405156538, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71994694, Length=594, Percent_Identity=23.9057239057239, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71994690, Length=594, Percent_Identity=23.9057239057239, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI71994703, Length=548, Percent_Identity=23.9051094890511, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17538037, Length=539, Percent_Identity=22.8200371057514, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI71985884, Length=368, Percent_Identity=25.5434782608696, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17558820, Length=517, Percent_Identity=25.9187620889749, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI32563687, Length=512, Percent_Identity=25.9765625, Blast_Score=109, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6319699, Length=380, Percent_Identity=25.5263157894737, Blast_Score=109, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6319264, Length=545, Percent_Identity=24.4036697247706, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323182, Length=571, Percent_Identity=22.5919439579685, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI18859661, Length=369, Percent_Identity=27.9132791327913, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI21355181, Length=543, Percent_Identity=25.2302025782689, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24581924, Length=568, Percent_Identity=25.1760563380282, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI20130357, Length=369, Percent_Identity=27.9132791327913, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI19922652, Length=521, Percent_Identity=22.2648752399232, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24653035, Length=378, Percent_Identity=25.9259259259259, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21356947, Length=372, Percent_Identity=24.7311827956989, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648260, Length=376, Percent_Identity=26.3297872340426, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21356441, Length=341, Percent_Identity=26.099706744868, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI161076582, Length=380, Percent_Identity=25, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24648257, Length=367, Percent_Identity=25.3405994550409, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648253, Length=361, Percent_Identity=24.0997229916898, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24648255, Length=362, Percent_Identity=24.3093922651934, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI21358303, Length=142, Percent_Identity=33.0985915492958, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281365686, Length=184, Percent_Identity=25.5434782608696, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 60605; Mature: 60605

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQNLIVSTPSAYSYPLLVKQLLIHSMSLYGDQEISYRGEMRYTYRDFRRRVGQLASTLA
CCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHH
SLGAKHGSTVAVMDWDSHRYLECYFGVPMMGATLFTVNVRLSPQQILYTLNDADADIVLV
HHCCCCCCEEEEEECCCCCEEEEEECCCHHCCEEEEEEEEECHHHEEEEECCCCCCEEEE
HPDFVPVLDQIRNELTCNPRFVLLADGHAAPQTELQFAGEYEALLADASPDFDFPEFDEN
CCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHEECCCHHEEEECCCCCCCCCCCCCC
TKAATFYTTGTTGDPKGVCYSHRDIVLHTLSTSASLCSPRDGQRMHREDVYMPITPMFHV
CCEEEEEECCCCCCCCCEEECCCCEEEEEECCCCCCCCCCCCCHHHHCCCCCCCHHHHHH
LAWGIPYLAVTLGLRTVLPGRYAPDVLLKLREAEKVTFSHCVPTILQMLLQSSQDSGQDL
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SGWKIIIGGSALPPSLCEAALDRGIDIFAGYGMSETGPIVSLAQLPPNVKPANRADEVRM
CCCEEEECCCCCCHHHHHHHHHCCCEEEECCCCCCCCCEEEHHHCCCCCCCCCCHHHHHE
RCSTGRPVVMADFRIVSEDMQDLPRDGKNRGEIVLRAPYLTQSYFKKPGASEELWAGGYL
EECCCCCEEEEHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCCCCE
HTQDVAVMGEDGFVQIVDRIKDVIKTGGEWVSSIEVENLISEVPGVQECAVIGVPDDKWG
EECCEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
ERPMAFVVRKAGAEVGADSIREKLLGHVDSRRISKYAVPEAERIAFVAEIPKTSVGKINK
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCCCCCCH
KQLREKAA
HHHHHHCC
>Mature Secondary Structure
MQQNLIVSTPSAYSYPLLVKQLLIHSMSLYGDQEISYRGEMRYTYRDFRRRVGQLASTLA
CCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHH
SLGAKHGSTVAVMDWDSHRYLECYFGVPMMGATLFTVNVRLSPQQILYTLNDADADIVLV
HHCCCCCCEEEEEECCCCCEEEEEECCCHHCCEEEEEEEEECHHHEEEEECCCCCCEEEE
HPDFVPVLDQIRNELTCNPRFVLLADGHAAPQTELQFAGEYEALLADASPDFDFPEFDEN
CCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHEECCCHHEEEECCCCCCCCCCCCCC
TKAATFYTTGTTGDPKGVCYSHRDIVLHTLSTSASLCSPRDGQRMHREDVYMPITPMFHV
CCEEEEEECCCCCCCCCEEECCCCEEEEEECCCCCCCCCCCCCHHHHCCCCCCCHHHHHH
LAWGIPYLAVTLGLRTVLPGRYAPDVLLKLREAEKVTFSHCVPTILQMLLQSSQDSGQDL
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SGWKIIIGGSALPPSLCEAALDRGIDIFAGYGMSETGPIVSLAQLPPNVKPANRADEVRM
CCCEEEECCCCCCHHHHHHHHHCCCEEEECCCCCCCCCEEEHHHCCCCCCCCCCHHHHHE
RCSTGRPVVMADFRIVSEDMQDLPRDGKNRGEIVLRAPYLTQSYFKKPGASEELWAGGYL
EECCCCCEEEEHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCCCCE
HTQDVAVMGEDGFVQIVDRIKDVIKTGGEWVSSIEVENLISEVPGVQECAVIGVPDDKWG
EECCEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
ERPMAFVVRKAGAEVGADSIREKLLGHVDSRRISKYAVPEAERIAFVAEIPKTSVGKINK
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCCCCCCH
KQLREKAA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]