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The map label for this gene is fabI [H]

Identifier: 73541818

GI number: 73541818

Start: 2350299

End: 2351081

Strand: Direct

Name: fabI [H]

Synonym: Reut_A2130

Alternate gene names: 73541818

Gene position: 2350299-2351081 (Clockwise)

Preceding gene: 73541812

Following gene: 73541820

Centisome position: 61.74

GC content: 64.24

Gene sequence:

>783_bases
ATGGGATTTCTGGCAGGTAAGCGGATCCTCATCACCGGCTTGCTATCGAACCGTTCGATCGCCTATGGCATCGCATCGGC
CTGCAAACGCGAAGGCGCCGAACTCGCCTTCACCTACGTCGGCGAACGGTTCAAGGACCGGATCAGCGACTTTGCCAAGG
AATTCGGCACCGATCTCGTCTTCGAATGCGACGTCGGCAGCGACGAGCAGATCGCCGCAACCTTCGACGGTCTCGGCAAG
CGCTGGGAAAAGCTCGACGGCCTGGTGCACTCGATCGGTTTCGCCCCGCGCGAAGCCATTGCCGGCGATTTCCTGGAAGG
CCTGTCGCGCGAAGGCTTCCGCATTGCCCATGACATCTCCGCATACAGCTTCCCGGCGCTGGCCAAGGCCGCGCTGCCGA
TGCTGTCGGACAAGGCTTCGCTGCTGACGCTGACCTATCTCGGCGCCGAGCGCGTGGTCCCGAACTACAACACCATGGGC
CTGGCCAAGGCCGCGCTTGAGGCGAGCGTGCGCTACCTGGCGGCGTCGGTCGGCCCGCGCGGCATCCGCGCGAACGGCAT
TTCGGCCGGCCCGATCAAGACCCTGGCTGCGTCCGGCATCAAGGACTTCGGCAAGCTGCTCAATGCCTTCGAAAACTCGG
CCCCGCTGCGTCGCAACGTGACCATCGAGGAAGTCGGCAATGTCGCGGCCTTCCTGCTGTCCGACCTCGCCAGCGGCGTG
ACCGGCGAGATTACCTACGTCGACGCCGGCTTCAACATTGTTGGCGCGGGTCTGCCGGAGTAA

Upstream 100 bases:

>100_bases
CTCTACACGGCGCAATAAAGTGGCCGTCGGACACCGGGGCATGCCCACGGCCGCGTGGCCGCAGCAGGAAGCGTGCCCGA
TACAACACCTTGGAGAACTT

Downstream 100 bases:

>100_bases
TCAGCGCAAACAGCGCAAGCTCAAACCCGCCAACCGGCCCTGAAGGGGTGCCAGCGCTTGCTGGCACCCCTTTTTCATTG
GGTCAGCCCGGGCGCGTCCG

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MGFLAGKRILITGLLSNRSIAYGIASACKREGAELAFTYVGERFKDRISDFAKEFGTDLVFECDVGSDEQIAATFDGLGK
RWEKLDGLVHSIGFAPREAIAGDFLEGLSREGFRIAHDISAYSFPALAKAALPMLSDKASLLTLTYLGAERVVPNYNTMG
LAKAALEASVRYLAASVGPRGIRANGISAGPIKTLAASGIKDFGKLLNAFENSAPLRRNVTIEEVGNVAAFLLSDLASGV
TGEITYVDAGFNIVGAGLPE

Sequences:

>Translated_260_residues
MGFLAGKRILITGLLSNRSIAYGIASACKREGAELAFTYVGERFKDRISDFAKEFGTDLVFECDVGSDEQIAATFDGLGK
RWEKLDGLVHSIGFAPREAIAGDFLEGLSREGFRIAHDISAYSFPALAKAALPMLSDKASLLTLTYLGAERVVPNYNTMG
LAKAALEASVRYLAASVGPRGIRANGISAGPIKTLAASGIKDFGKLLNAFENSAPLRRNVTIEEVGNVAAFLLSDLASGV
TGEITYVDAGFNIVGAGLPE
>Mature_259_residues
GFLAGKRILITGLLSNRSIAYGIASACKREGAELAFTYVGERFKDRISDFAKEFGTDLVFECDVGSDEQIAATFDGLGKR
WEKLDGLVHSIGFAPREAIAGDFLEGLSREGFRIAHDISAYSFPALAKAALPMLSDKASLLTLTYLGAERVVPNYNTMGL
AKAALEASVRYLAASVGPRGIRANGISAGPIKTLAASGIKDFGKLLNAFENSAPLRRNVTIEEVGNVAAFLLSDLASGVT
GEITYVDAGFNIVGAGLPE

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=253, Percent_Identity=61.2648221343874, Blast_Score=328, Evalue=2e-91,
Organism=Escherichia coli, GI87082100, Length=269, Percent_Identity=28.6245353159851, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789378, Length=255, Percent_Identity=28.6274509803922, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787905, Length=250, Percent_Identity=26.8, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI2367365, Length=252, Percent_Identity=28.968253968254, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27490; Mature: 27358

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFLAGKRILITGLLSNRSIAYGIASACKREGAELAFTYVGERFKDRISDFAKEFGTDLV
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
FECDVGSDEQIAATFDGLGKRWEKLDGLVHSIGFAPREAIAGDFLEGLSREGFRIAHDIS
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEHHCC
AYSFPALAKAALPMLSDKASLLTLTYLGAERVVPNYNTMGLAKAALEASVRYLAASVGPR
HHHHHHHHHHHHHHHCCCHHEEEEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
GIRANGISAGPIKTLAASGIKDFGKLLNAFENSAPLRRNVTIEEVGNVAAFLLSDLASGV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
TGEITYVDAGFNIVGAGLPE
CCEEEEEECCCEEECCCCCC
>Mature Secondary Structure 
GFLAGKRILITGLLSNRSIAYGIASACKREGAELAFTYVGERFKDRISDFAKEFGTDLV
CCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
FECDVGSDEQIAATFDGLGKRWEKLDGLVHSIGFAPREAIAGDFLEGLSREGFRIAHDIS
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEHHCC
AYSFPALAKAALPMLSDKASLLTLTYLGAERVVPNYNTMGLAKAALEASVRYLAASVGPR
HHHHHHHHHHHHHHHCCCHHEEEEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
GIRANGISAGPIKTLAASGIKDFGKLLNAFENSAPLRRNVTIEEVGNVAAFLLSDLASGV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
TGEITYVDAGFNIVGAGLPE
CCEEEEEECCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]