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The map label for this gene is dmlR [H]

Identifier: 73541641

GI number: 73541641

Start: 2139809

End: 2140723

Strand: Direct

Name: dmlR [H]

Synonym: Reut_A1953

Alternate gene names: 73541641

Gene position: 2139809-2140723 (Clockwise)

Preceding gene: 73541635

Following gene: 73541642

Centisome position: 56.21

GC content: 64.59

Gene sequence:

>915_bases
GTGGCCTCGCGCGGCAGCTTGTCCGCCGCGGCGGGAGCCGAAGGGGTGGCACCCGCCATCATCGGACGGCGCATCGATGC
CCTGGAGGAGCGGCTGGGCGTCAAGCTGTTGATCCGCACCACGCGCAAGATCAGCCTGACCTTCGAGGGTTCAGCCTTCC
TGGAAGACTGCCAGCGCATCCTGAATGACCTGCACAACGCGGAAGCCAGCGTGTCGGCCGGTGGCGTAAAGGCCAGCGGC
CACCTGCGTGTCACGGCCCCGGCCGGTTACGGACGCAAGCATGTGGCGCCGCTCGTGCCGCTGTTTATCGAATCGCACCC
TGATGTGACGATTACGCTGGATCTGTCCGATCGCGTCGTCGATCTCGTCAATGAAGGCTTTGACTGCGCCATTCGGCTGG
GCGATCTGCCGGATTCCAGCCTGGTTTCCATCCGGCTCGCCGAAACACGTCGTGTGGTGGTGGCATCACCCGACTATCTG
GCAAGGGCAGGCACTCCGGTTCTGCCGGAAGAACTGCAGGGCCATAACTGCCTGGCGTTCGGCGCGAGCGCCAATGTCCA
GCGCGGCTGGGTATTCCAGCAGGAAGGCCATACGGTCACGGTCAAAGTGGGCGGCGATATGGAATGCACGGACGGCGCCG
TGCTCCACGAGTGGTGCCTGCAGGGGCATGGATTGGCCTGGCGTTCGTGGTGGGAGGTGGGCAGCGAAATCGCCAGCGGC
CGGCTGGTGACAGTGCTCGATGAGTTCCAGGCGCCGCCGATCGGCATCCACGCCGTATTTCCGCAGCGCAAGCACCTGCC
GCTGCGCGTGCGCTTGTTTATCGACCATCTGAAGAACACTTACGGCAATCCGGCCTACTGGCGCCGGACTGAGCAGACTC
CGGCTCGGACAGCCATGACGGCAGTGGCGGGATAA

Upstream 100 bases:

>100_bases
TTGCGTGAAATCCCCGTCCACCAGCCCGTGCGGGTGATCGCGCAGGCATCGCAGGCAAACATTTGTGGACCATTTCAAAC
AACTGGAGACTTTTGTATCC

Downstream 100 bases:

>100_bases
GGCAGCTACGCGGGAGCAGGATGTGCGCTATCGCACTCTGTTGCAATTGATTTAGCGCAATGCCCTACTCGGCACACTGT
CTACAATGGTTTTCAGACAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MASRGSLSAAAGAEGVAPAIIGRRIDALEERLGVKLLIRTTRKISLTFEGSAFLEDCQRILNDLHNAEASVSAGGVKASG
HLRVTAPAGYGRKHVAPLVPLFIESHPDVTITLDLSDRVVDLVNEGFDCAIRLGDLPDSSLVSIRLAETRRVVVASPDYL
ARAGTPVLPEELQGHNCLAFGASANVQRGWVFQQEGHTVTVKVGGDMECTDGAVLHEWCLQGHGLAWRSWWEVGSEIASG
RLVTVLDEFQAPPIGIHAVFPQRKHLPLRVRLFIDHLKNTYGNPAYWRRTEQTPARTAMTAVAG

Sequences:

>Translated_304_residues
MASRGSLSAAAGAEGVAPAIIGRRIDALEERLGVKLLIRTTRKISLTFEGSAFLEDCQRILNDLHNAEASVSAGGVKASG
HLRVTAPAGYGRKHVAPLVPLFIESHPDVTITLDLSDRVVDLVNEGFDCAIRLGDLPDSSLVSIRLAETRRVVVASPDYL
ARAGTPVLPEELQGHNCLAFGASANVQRGWVFQQEGHTVTVKVGGDMECTDGAVLHEWCLQGHGLAWRSWWEVGSEIASG
RLVTVLDEFQAPPIGIHAVFPQRKHLPLRVRLFIDHLKNTYGNPAYWRRTEQTPARTAMTAVAG
>Mature_303_residues
ASRGSLSAAAGAEGVAPAIIGRRIDALEERLGVKLLIRTTRKISLTFEGSAFLEDCQRILNDLHNAEASVSAGGVKASGH
LRVTAPAGYGRKHVAPLVPLFIESHPDVTITLDLSDRVVDLVNEGFDCAIRLGDLPDSSLVSIRLAETRRVVVASPDYLA
RAGTPVLPEELQGHNCLAFGASANVQRGWVFQQEGHTVTVKVGGDMECTDGAVLHEWCLQGHGLAWRSWWEVGSEIASGR
LVTVLDEFQAPPIGIHAVFPQRKHLPLRVRLFIDHLKNTYGNPAYWRRTEQTPARTAMTAVAG

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=282, Percent_Identity=35.8156028368794, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI1786401, Length=274, Percent_Identity=35.036496350365, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1789440, Length=274, Percent_Identity=31.021897810219, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI145693193, Length=273, Percent_Identity=31.5018315018315, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787589, Length=280, Percent_Identity=30, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1787128, Length=280, Percent_Identity=27.1428571428571, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1789639, Length=277, Percent_Identity=29.2418772563177, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1786448, Length=264, Percent_Identity=28.4090909090909, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790011, Length=296, Percent_Identity=25.6756756756757, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1788706, Length=161, Percent_Identity=27.3291925465839, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789173, Length=243, Percent_Identity=26.3374485596708, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32917; Mature: 32786

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRGSLSAAAGAEGVAPAIIGRRIDALEERLGVKLLIRTTRKISLTFEGSAFLEDCQRI
CCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHCCEEEEEEECEEEEEEECCCHHHHHHHHH
LNDLHNAEASVSAGGVKASGHLRVTAPAGYGRKHVAPLVPLFIESHPDVTITLDLSDRVV
HHHHHCCCCCCCCCCEEECCEEEEEECCCCCCCHHHHHHHHEECCCCCEEEEEECCHHHH
DLVNEGFDCAIRLGDLPDSSLVSIRLAETRRVVVASPDYLARAGTPVLPEELQGHNCLAF
HHHCCCCEEEEEECCCCCCCEEEEEEECCCEEEEECCCHHHHCCCCCCHHHHCCCEEEEE
GASANVQRGWVFQQEGHTVTVKVGGDMECTDGAVLHEWCLQGHGLAWRSWWEVGSEIASG
CCCCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCC
RLVTVLDEFQAPPIGIHAVFPQRKHLPLRVRLFIDHLKNTYGNPAYWRRTEQTPARTAMT
CEEEEEHHHCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHCCCCHHEEECCCCCHHHHHH
AVAG
HHCC
>Mature Secondary Structure 
ASRGSLSAAAGAEGVAPAIIGRRIDALEERLGVKLLIRTTRKISLTFEGSAFLEDCQRI
CCCCCCCHHCCCCCCCHHHHHHHHHHHHHHCCEEEEEEECEEEEEEECCCHHHHHHHHH
LNDLHNAEASVSAGGVKASGHLRVTAPAGYGRKHVAPLVPLFIESHPDVTITLDLSDRVV
HHHHHCCCCCCCCCCEEECCEEEEEECCCCCCCHHHHHHHHEECCCCCEEEEEECCHHHH
DLVNEGFDCAIRLGDLPDSSLVSIRLAETRRVVVASPDYLARAGTPVLPEELQGHNCLAF
HHHCCCCEEEEEECCCCCCCEEEEEEECCCEEEEECCCHHHHCCCCCCHHHHCCCEEEEE
GASANVQRGWVFQQEGHTVTVKVGGDMECTDGAVLHEWCLQGHGLAWRSWWEVGSEIASG
CCCCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCC
RLVTVLDEFQAPPIGIHAVFPQRKHLPLRVRLFIDHLKNTYGNPAYWRRTEQTPARTAMT
CEEEEEHHHCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHCCCCHHEEECCCCCHHHHHH
AVAG
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]