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The map label for this gene is htrB [H]

Identifier: 73541635

GI number: 73541635

Start: 2133530

End: 2134432

Strand: Direct

Name: htrB [H]

Synonym: Reut_A1946

Alternate gene names: 73541635

Gene position: 2133530-2134432 (Clockwise)

Preceding gene: 73541634

Following gene: 73541641

Centisome position: 56.05

GC content: 65.23

Gene sequence:

>903_bases
GTGACCGACTCCCCGCGCCAGCCTTCCTCACGCAGCCCTCTTTCCATGGTGGTACGGCTCGTTGCCGCGCTGCCGCTGCC
GGTCGCCCATGCAGTCGGACGCATGTTGGGACGGCTCGTCTATGCCCTGCCGGGCCGGTACCGCCAGCATGTGCAACAGA
ATGCGGCCCAAGCCGGATATCCGGGGCCTGCTTTCGCACGCCGCGCGGCGGCCGAAACCGGTGCGATGATGACCGAACTG
GCCCATGTCTGGTTTCGCAGCGCGGACTGTATGGAGAAGGTCATCTGCGAAGACTGGGACGTGATCGAGCAAACGCTCGC
CGAAGGCCGTGGTCTCGTCATGCTTACGCCTCACCTGGGATGCTTCGAACTGGCGGCCCGCTATGCCGCGGAACGCATCT
CGTTGACGATCATGTTCCGGCCGCACCGCAAGGCAGTGTTTCGGGAATTGCTCGACTCCGTGCGCGAAACGTCGAACCTG
AAGGCGTTTCCGGCGACCGCACAGGGGGTACGTGCGTTCGTACGCGCATTGCGGCAGGGCGAGGCGGTCGGTCTGTTGCC
CGACCAGGCCCCCAAGGGCGATGGCGTGTGGGTGCCCTTCTTCGGCCGCAAGGCCTATACGATCACCTTGCCGTGCAAGC
TGGCATCCCAAACTGGTGCGCCATTGATCATCGCCGCCGGCGAGCGGCTGCCGCATGGGCGCGGCTGGCGCATGCATGTG
AAGCGACTGACGGCGCCGATGCCGCCGACCGCGGAAGAACAGGCAGCGTGGATCAATGCCGAGGTCGAGGAAATGATTCG
CCGATTCCCCGAGCAGTATCTGTGGGGTTACAACCGCTACAAGGTACCGTCCGGCACGCAAGCGCCGAGCGCGAACAGCA
CCGAGACCGCAAACGCCGCGTGA

Upstream 100 bases:

>100_bases
GCCCGCAAACCGATGCGTGGTACAGTGAGCGCCCTTCAACGCGAGAGGCCCGCATGTTCCGCCGCGCCAATGATTTCACG
CTGAAAAGGGAGACGCTCGC

Downstream 100 bases:

>100_bases
CGACTGCGCTCGCGGCGCATTCCTCGCTCACTTGAGGTATTTCATCGTCGCCACGCCCGTGGCGACGAGCGTTTCTTCGT
CCAGCCAGACCTCCGCGCGC

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MTDSPRQPSSRSPLSMVVRLVAALPLPVAHAVGRMLGRLVYALPGRYRQHVQQNAAQAGYPGPAFARRAAAETGAMMTEL
AHVWFRSADCMEKVICEDWDVIEQTLAEGRGLVMLTPHLGCFELAARYAAERISLTIMFRPHRKAVFRELLDSVRETSNL
KAFPATAQGVRAFVRALRQGEAVGLLPDQAPKGDGVWVPFFGRKAYTITLPCKLASQTGAPLIIAAGERLPHGRGWRMHV
KRLTAPMPPTAEEQAAWINAEVEEMIRRFPEQYLWGYNRYKVPSGTQAPSANSTETANAA

Sequences:

>Translated_300_residues
MTDSPRQPSSRSPLSMVVRLVAALPLPVAHAVGRMLGRLVYALPGRYRQHVQQNAAQAGYPGPAFARRAAAETGAMMTEL
AHVWFRSADCMEKVICEDWDVIEQTLAEGRGLVMLTPHLGCFELAARYAAERISLTIMFRPHRKAVFRELLDSVRETSNL
KAFPATAQGVRAFVRALRQGEAVGLLPDQAPKGDGVWVPFFGRKAYTITLPCKLASQTGAPLIIAAGERLPHGRGWRMHV
KRLTAPMPPTAEEQAAWINAEVEEMIRRFPEQYLWGYNRYKVPSGTQAPSANSTETANAA
>Mature_299_residues
TDSPRQPSSRSPLSMVVRLVAALPLPVAHAVGRMLGRLVYALPGRYRQHVQQNAAQAGYPGPAFARRAAAETGAMMTELA
HVWFRSADCMEKVICEDWDVIEQTLAEGRGLVMLTPHLGCFELAARYAAERISLTIMFRPHRKAVFRELLDSVRETSNLK
AFPATAQGVRAFVRALRQGEAVGLLPDQAPKGDGVWVPFFGRKAYTITLPCKLASQTGAPLIIAAGERLPHGRGWRMHVK
RLTAPMPPTAEEQAAWINAEVEEMIRRFPEQYLWGYNRYKVPSGTQAPSANSTETANAA

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=284, Percent_Identity=28.8732394366197, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI87082094, Length=276, Percent_Identity=25.3623188405797, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 33088; Mature: 32956

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSPRQPSSRSPLSMVVRLVAALPLPVAHAVGRMLGRLVYALPGRYRQHVQQNAAQAGY
CCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
PGPAFARRAAAETGAMMTELAHVWFRSADCMEKVICEDWDVIEQTLAEGRGLVMLTPHLG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEECCCCC
CFELAARYAAERISLTIMFRPHRKAVFRELLDSVRETSNLKAFPATAQGVRAFVRALRQG
HHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCC
EAVGLLPDQAPKGDGVWVPFFGRKAYTITLPCKLASQTGAPLIIAAGERLPHGRGWRMHV
CEEECCCCCCCCCCCEEEEEECCEEEEEEECCEECCCCCCCEEEECCCCCCCCCCHHHHH
KRLTAPMPPTAEEQAAWINAEVEEMIRRFPEQYLWGYNRYKVPSGTQAPSANSTETANAA
HHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDSPRQPSSRSPLSMVVRLVAALPLPVAHAVGRMLGRLVYALPGRYRQHVQQNAAQAGY
CCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
PGPAFARRAAAETGAMMTELAHVWFRSADCMEKVICEDWDVIEQTLAEGRGLVMLTPHLG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEECCCCC
CFELAARYAAERISLTIMFRPHRKAVFRELLDSVRETSNLKAFPATAQGVRAFVRALRQG
HHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCC
EAVGLLPDQAPKGDGVWVPFFGRKAYTITLPCKLASQTGAPLIIAAGERLPHGRGWRMHV
CEEECCCCCCCCCCCEEEEEECCEEEEEEECCEECCCCCCCEEEECCCCCCCCCCHHHHH
KRLTAPMPPTAEEQAAWINAEVEEMIRRFPEQYLWGYNRYKVPSGTQAPSANSTETANAA
HHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]