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The map label for this gene is smpB [H]

Identifier: 73541550

GI number: 73541550

Start: 2036814

End: 2037266

Strand: Direct

Name: smpB [H]

Synonym: Reut_A1861

Alternate gene names: 73541550

Gene position: 2036814-2037266 (Clockwise)

Preceding gene: 73541547

Following gene: 73541554

Centisome position: 53.51

GC content: 60.49

Gene sequence:

>453_bases
ATGACCATCGCAGATAACAAGAAGGCCTTTTTCGACTACTTCATTGAAGAGCGATTCGAGGCCGGCATCGTGCTGGAAGG
CTGGGAAGTCAAGGCGATCCGCGCCGGACGCGTGCAGATCAAGGAAGGTTACGTGGTCGTGCGCGACGCCGAAATGTTCC
TGATCGGCGCCCACATCAGCCCGCTGCAGAGCGCCTCCACCCACGTGAATCCGGACCCGGTGCGCACGCGCAAGCTGCTG
CTCAAGGCCGACGAGATCAAGAAGCTGATCGGGAAGGTCGAGCAGCGTGGCTATACGCTGGTGCCGCTGAACCTGCACTA
CACGCGCGGCCGCGTGAAATGCGAGATCGGCCTCGGCAAGGGCAAGAAACTGTTCGACAAGCGCGAAACCGAGAAGCAAC
GCGACTGGCAGCGCGAGAAGTCACGCATCATGAAGGGCGGCAGCAAGGAGTAA

Upstream 100 bases:

>100_bases
AAATGGGGATTTTAGCCGACCCTGCCGCCCGAGGCACCCCCGCCTCAGAACGGGCCAATCACGGCCAGCCATTCCACTTA
CGCATCCCTGACCCGCACGC

Downstream 100 bases:

>100_bases
CGCGCTCGGGCCCCGCAGTTTCGCTACGTTCTCAGACGGCCTTCTGGCCTTTCACAATCTGCAGCGCCGAGGCAATCATG
CTGCTCATCTCGCCGAGGTC

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 150; Mature: 149

Protein sequence:

>150_residues
MTIADNKKAFFDYFIEERFEAGIVLEGWEVKAIRAGRVQIKEGYVVVRDAEMFLIGAHISPLQSASTHVNPDPVRTRKLL
LKADEIKKLIGKVEQRGYTLVPLNLHYTRGRVKCEIGLGKGKKLFDKRETEKQRDWQREKSRIMKGGSKE

Sequences:

>Translated_150_residues
MTIADNKKAFFDYFIEERFEAGIVLEGWEVKAIRAGRVQIKEGYVVVRDAEMFLIGAHISPLQSASTHVNPDPVRTRKLL
LKADEIKKLIGKVEQRGYTLVPLNLHYTRGRVKCEIGLGKGKKLFDKRETEKQRDWQREKSRIMKGGSKE
>Mature_149_residues
TIADNKKAFFDYFIEERFEAGIVLEGWEVKAIRAGRVQIKEGYVVVRDAEMFLIGAHISPLQSASTHVNPDPVRTRKLLL
KADEIKKLIGKVEQRGYTLVPLNLHYTRGRVKCEIGLGKGKKLFDKRETEKQRDWQREKSRIMKGGSKE

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=148, Percent_Identity=53.3783783783784, Blast_Score=166, Evalue=7e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 17273; Mature: 17142

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIADNKKAFFDYFIEERFEAGIVLEGWEVKAIRAGRVQIKEGYVVVRDAEMFLIGAHIS
CCCCCCCHHHHHHHHHHHCCCCEEEECCEEEEEECCEEEEECCEEEEEECEEEEEECCCC
PLQSASTHVNPDPVRTRKLLLKADEIKKLIGKVEQRGYTLVPLNLHYTRGRVKCEIGLGK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCEEEEEEECCC
GKKLFDKRETEKQRDWQREKSRIMKGGSKE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TIADNKKAFFDYFIEERFEAGIVLEGWEVKAIRAGRVQIKEGYVVVRDAEMFLIGAHIS
CCCCCCHHHHHHHHHHHCCCCEEEECCEEEEEECCEEEEECCEEEEEECEEEEEECCCC
PLQSASTHVNPDPVRTRKLLLKADEIKKLIGKVEQRGYTLVPLNLHYTRGRVKCEIGLGK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCEEEEEEECCC
GKKLFDKRETEKQRDWQREKSRIMKGGSKE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA