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The map label for this gene is citA [H]

Identifier: 73541060

GI number: 73541060

Start: 1469504

End: 1470811

Strand: Reverse

Name: citA [H]

Synonym: Reut_A1366

Alternate gene names: 73541060

Gene position: 1470811-1469504 (Counterclockwise)

Preceding gene: 73541061

Following gene: 73541059

Centisome position: 38.64

GC content: 63.84

Gene sequence:

>1308_bases
ATGTCAGACACCTCCCAAACCGCCGGCCAACCGCAAGCGCAGCACGGATTCAAGACCGTCTTCCGCGTCGTCAGTGGCAA
TTTCCTGGAAATGTACGACTTCATGGTGTACGGCTTCTACGCCGCCGCCATTGCCAGGACTTTCTTCCCGAGCGGCGACG
AATTTGCGTCGCTGATGCTGTCGCTGGCGACGTTTGGCGCCGGCTTCCTGATGCGTCCGCTGGGCGCCATCGTGCTGGGC
GCGTACATCGACCATCACGGCCGCCGCAAGGGGCTGATCGTCACGCTCGCGCTGATGGCCGCCGGCACGCTGCTGATCGC
ATGCGTGCCCGGCTATGCCTCGATCGGCGTGGCGGCACCGCTGCTCGTGCTGGCCGGCCGCCTGTTGCAGGGCTTCTCTG
CGGGCGTGGAACTGGGCGGCGTCTCGGTCTACCTGTCGGAGATCGCCAAACCTGGCAAGAAAGGCTTTTACGTAGCCTGG
CAGTCTGCCAGCCAGCAAGTCGCGGTGATCTTCGCCGGCCTGCTTGGCGTGATCCTGCATGCGACGCTTGCACCGCAGCA
AATGGATGACTGGGGCTGGCGCGTGCCGTTCCTGATCGGGTGCCTGATCGTGCCGTTCCTGTTCCTGATCCGCCGCTCGC
TCGAAGAAACCGAGGAATTCAAGACCCGCAAGCATCGCCCGACCATGCGCGAGATCTACCGCTCGATGGCCGAGAACTGG
CGCATCATCATTGCGGGCTGCATGATGGTCGTGATGACGACGGTATCGTTCTACATGATCACCGCATACACCCCGACCTT
CGGCAAAACGGTGCTCAAGCTCGATGACGTGGACAACCTGATCGTCACCATGTGTGTGGGCCTGTCGAACTTCATCTGGC
TGCCGCTGATGGGCGCGGTGTCCGACCGCATCGGTCGCAAGCCGCTGCTGGTGTTCTTCACGCTGGCCACGCTGCTGACC
GCCTATCCCGCCGTCTCGTGGCTGGTGGCCGAGCCGTCGTTCTCGCGCCTGCTGATGGTCGAACTCTGGCTGTCGTTCCT
GTACGGCAGCTATAACGGCGCGATGGTAGTCACGCTAACCGAAGTCATGCCGCCGGCGGTGCGTACCGCGGGTTTCTCGC
TGGCCTATAGCCTGGCTACGGCGCTGTTCGGCGGATTCACGCCGGCAATCGCGACCTACCTGATCCACACCACCGGTAAC
AAGGCGGCCCCTGGCCTGTGGCTGATGTTCGCCGCCGCCTGCGGCCTGATCGCCACCCTGATCATTTTCCGCGCACGTCG
CGGCCAGCCCGCGCTGCGTCCTGCCTGA

Upstream 100 bases:

>100_bases
TCCCAGCCACGGCGACGCCTGCTGTCTCCTCCCAACGCCTGGCTGGCTCACCGAACAATCATTCGCCGGGACCCACCGGC
GACGTCCGAGAGAGACAGCC

Downstream 100 bases:

>100_bases
GCGGCCACCTGCCGTCCCTGCCACACCGGCCTTGTGCGGCCGGTCGTGGCATACTTCCCGCTTGCGCAAACTCCCATGCG
CGACGATCCAGAGCGCCATG

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSDTSQTAGQPQAQHGFKTVFRVVSGNFLEMYDFMVYGFYAAAIARTFFPSGDEFASLMLSLATFGAGFLMRPLGAIVLG
AYIDHHGRRKGLIVTLALMAAGTLLIACVPGYASIGVAAPLLVLAGRLLQGFSAGVELGGVSVYLSEIAKPGKKGFYVAW
QSASQQVAVIFAGLLGVILHATLAPQQMDDWGWRVPFLIGCLIVPFLFLIRRSLEETEEFKTRKHRPTMREIYRSMAENW
RIIIAGCMMVVMTTVSFYMITAYTPTFGKTVLKLDDVDNLIVTMCVGLSNFIWLPLMGAVSDRIGRKPLLVFFTLATLLT
AYPAVSWLVAEPSFSRLLMVELWLSFLYGSYNGAMVVTLTEVMPPAVRTAGFSLAYSLATALFGGFTPAIATYLIHTTGN
KAAPGLWLMFAAACGLIATLIIFRARRGQPALRPA

Sequences:

>Translated_435_residues
MSDTSQTAGQPQAQHGFKTVFRVVSGNFLEMYDFMVYGFYAAAIARTFFPSGDEFASLMLSLATFGAGFLMRPLGAIVLG
AYIDHHGRRKGLIVTLALMAAGTLLIACVPGYASIGVAAPLLVLAGRLLQGFSAGVELGGVSVYLSEIAKPGKKGFYVAW
QSASQQVAVIFAGLLGVILHATLAPQQMDDWGWRVPFLIGCLIVPFLFLIRRSLEETEEFKTRKHRPTMREIYRSMAENW
RIIIAGCMMVVMTTVSFYMITAYTPTFGKTVLKLDDVDNLIVTMCVGLSNFIWLPLMGAVSDRIGRKPLLVFFTLATLLT
AYPAVSWLVAEPSFSRLLMVELWLSFLYGSYNGAMVVTLTEVMPPAVRTAGFSLAYSLATALFGGFTPAIATYLIHTTGN
KAAPGLWLMFAAACGLIATLIIFRARRGQPALRPA
>Mature_434_residues
SDTSQTAGQPQAQHGFKTVFRVVSGNFLEMYDFMVYGFYAAAIARTFFPSGDEFASLMLSLATFGAGFLMRPLGAIVLGA
YIDHHGRRKGLIVTLALMAAGTLLIACVPGYASIGVAAPLLVLAGRLLQGFSAGVELGGVSVYLSEIAKPGKKGFYVAWQ
SASQQVAVIFAGLLGVILHATLAPQQMDDWGWRVPFLIGCLIVPFLFLIRRSLEETEEFKTRKHRPTMREIYRSMAENWR
IIIAGCMMVVMTTVSFYMITAYTPTFGKTVLKLDDVDNLIVTMCVGLSNFIWLPLMGAVSDRIGRKPLLVFFTLATLLTA
YPAVSWLVAEPSFSRLLMVELWLSFLYGSYNGAMVVTLTEVMPPAVRTAGFSLAYSLATALFGGFTPAIATYLIHTTGNK
AAPGLWLMFAAACGLIATLIIFRARRGQPALRPA

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=408, Percent_Identity=30.1470588235294, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI1788942, Length=411, Percent_Identity=34.3065693430657, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1788292, Length=422, Percent_Identity=28.6729857819905, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1789941, Length=411, Percent_Identity=30.4136253041363, Blast_Score=148, Evalue=8e-37,
Organism=Escherichia coli, GI87082231, Length=232, Percent_Identity=27.5862068965517, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082404, Length=387, Percent_Identity=25.5813953488372, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47332; Mature: 47200

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCCCCCCCC
>Mature Secondary Structure 
SDTSQTAGQPQAQHGFKTVFRVVSGNFLEMYDFMVYGFYAAAIARTFFPSGDEFASLML
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
SLATFGAGFLMRPLGAIVLGAYIDHHGRRKGLIVTLALMAAGTLLIACVPGYASIGVAAP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LLVLAGRLLQGFSAGVELGGVSVYLSEIAKPGKKGFYVAWQSASQQVAVIFAGLLGVILH
HHHHHHHHHHHHHCCCHHCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHH
ATLAPQQMDDWGWRVPFLIGCLIVPFLFLIRRSLEETEEFKTRKHRPTMREIYRSMAENW
HHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
RIIIAGCMMVVMTTVSFYMITAYTPTFGKTVLKLDDVDNLIVTMCVGLSNFIWLPLMGAV
EEHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
SDRIGRKPLLVFFTLATLLTAYPAVSWLVAEPSFSRLLMVELWLSFLYGSYNGAMVVTLT
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEH
EVMPPAVRTAGFSLAYSLATALFGGFTPAIATYLIHTTGNKAAPGLWLMFAAACGLIATL
HHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
IIFRARRGQPALRPA
HHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]