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The map label for this gene is degS [H]

Identifier: 73540910

GI number: 73540910

Start: 1300675

End: 1302222

Strand: Direct

Name: degS [H]

Synonym: Reut_A1215

Alternate gene names: 73540910

Gene position: 1300675-1302222 (Clockwise)

Preceding gene: 73540909

Following gene: 73540911

Centisome position: 34.17

GC content: 63.7

Gene sequence:

>1548_bases
ATGTCCAAAGGGATAAGTACGTATTCAATCCGGATGCGCGGGGCCCGACGTCGCGCAGGCCGCGCATTGCGCAGGCCGCA
GAACACCCAGGCGCAACTTGCCGGGATCATTCGCTCGTCCATGGAGGCTATCATTTCCGTTGACGCCGACCAGCGCGTGG
TGTTGTTCAATCCCATGGCGGAAACCTTGTTCGGCTGGCCGGCCGACCAGGCGATTGGCGAGCCGCTGACGCGCTTCATC
CCGGAGCGCTTCCGGGCGGCGCATGAGGCGCATGTACGGCGGTTCGGCATCACCGGCGTATCGGATCGCCAGATGGGGCG
CCAGCGCGCACTTTATGCCCTGCGCCGCGACGGCACCGAGTTTCCGATCGAAGCATCGATTTCACAAACGACGCACCGCG
GTTCGAAGTTGTTCACCGTCATGCTGCGCGACATCACGGAAAGAACGCGTGCCGAGAAAGCCATGCATGCCAGCCTCCAA
CGTGTCAGGGAAAGCGAAGCGCGGCTGGCCGGCATCATCCGTTCCTCGATGGAGGCCATCATTACGGTGGACAGCACCCA
GCACGTCATCCTGTTCAACCCCATGGCGGAAACCTTGTTCGGCTGGCCCGCCGACCAGGCCATTGGCCGGCCGCTGGGCG
ATTTCATTCCCGAGCGCTTTCGTGCTGCGCACGATGCGCATGTGCGCCGGTTCGGCGTGACAGGGGTATCCGACCGGCAG
ATGGGGCGCCAGCGCGCACTCTATGCGCTGCGCCGCGATGGCAGCGAGTTTCCCATCGAGGCATCGATTTCACAGATTGC
GGACGGCGGCGTCAGTCTCTATACGGTGATGCTGCGCGATATCACGGAACGCGTACGCGCCGAGCAGGCGCTGCGCCGAT
CGCGAGAGGAACTGCAGCAGCTTTCGGACAGCATCCTGGCCACCCGCGAAGAAGAGAAGCGCCGCATCGCGCGCGAACTC
CATGATGATCTGGGCCAGCGGCTGAGCGCGCTGAAGATGGACATGACGATGCTTGCCGCCGATGTCAGCGAGAGCAGGGA
CATCCGCGGAATCCTCGCGGACATCACCGACATGAACGGCGTCATTGACGACACGGTGGCGTCGGTTCGCCGCATTGCCA
GCGATCTGCGGCCAGCCCTGCTTGACGAGCTCGGCATGCTGACCGCCATCGAGTGGCTGGCGGCTGATTTTTCCAGCCGG
TATGGCCTGCCGGTGAGTGTGGATGGCGTGGATGCGGACGTGCCCGAGCAGACCGCCATCGCCATGTTTCGCATTGTGCA
GGAGGCGCTCAGCAATGTCGTCCGGCACGCTGACGCAACGGTCGTGGGCGTTTCACTTGCCCAGGCGGACGGGCAGATCG
AGTTGCGGGTTCAGGACAATGGCGGCGGATGGGACAAGAAGCCCGCCGGGACGGAGCCACGCAAATCGCTGGGGCTGCTG
GGAATCCGGGAGCGGGCGCGCTTGCTTGGCGGTGCCGTCGAAGTCGACAGCGAACCTGGCAAGGGATTCCGGCTGACCGT
AAAGATTCCTCTGGAAAAAGGGATATGA

Upstream 100 bases:

>100_bases
CATACTGTCGGCGGGATTGTCCGACGGCGGTTGTACATGGCGTCAGCACTCCTATAGTGTGTTCAGGATTGTTCAGCAAT
CTGCGGCCAGGGCGCCCGGT

Downstream 100 bases:

>100_bases
AATGACGCGCGTGCTCATTGCCGATGACCATGCCGTCGTGCGCGACGGTCTCAAGCATATCCTGGAGCGCGCCGGCAAAT
TCGATGTGGTAGGCGAAGCC

Product: PAS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MSKGISTYSIRMRGARRRAGRALRRPQNTQAQLAGIIRSSMEAIISVDADQRVVLFNPMAETLFGWPADQAIGEPLTRFI
PERFRAAHEAHVRRFGITGVSDRQMGRQRALYALRRDGTEFPIEASISQTTHRGSKLFTVMLRDITERTRAEKAMHASLQ
RVRESEARLAGIIRSSMEAIITVDSTQHVILFNPMAETLFGWPADQAIGRPLGDFIPERFRAAHDAHVRRFGVTGVSDRQ
MGRQRALYALRRDGSEFPIEASISQIADGGVSLYTVMLRDITERVRAEQALRRSREELQQLSDSILATREEEKRRIAREL
HDDLGQRLSALKMDMTMLAADVSESRDIRGILADITDMNGVIDDTVASVRRIASDLRPALLDELGMLTAIEWLAADFSSR
YGLPVSVDGVDADVPEQTAIAMFRIVQEALSNVVRHADATVVGVSLAQADGQIELRVQDNGGGWDKKPAGTEPRKSLGLL
GIRERARLLGGAVEVDSEPGKGFRLTVKIPLEKGI

Sequences:

>Translated_515_residues
MSKGISTYSIRMRGARRRAGRALRRPQNTQAQLAGIIRSSMEAIISVDADQRVVLFNPMAETLFGWPADQAIGEPLTRFI
PERFRAAHEAHVRRFGITGVSDRQMGRQRALYALRRDGTEFPIEASISQTTHRGSKLFTVMLRDITERTRAEKAMHASLQ
RVRESEARLAGIIRSSMEAIITVDSTQHVILFNPMAETLFGWPADQAIGRPLGDFIPERFRAAHDAHVRRFGVTGVSDRQ
MGRQRALYALRRDGSEFPIEASISQIADGGVSLYTVMLRDITERVRAEQALRRSREELQQLSDSILATREEEKRRIAREL
HDDLGQRLSALKMDMTMLAADVSESRDIRGILADITDMNGVIDDTVASVRRIASDLRPALLDELGMLTAIEWLAADFSSR
YGLPVSVDGVDADVPEQTAIAMFRIVQEALSNVVRHADATVVGVSLAQADGQIELRVQDNGGGWDKKPAGTEPRKSLGLL
GIRERARLLGGAVEVDSEPGKGFRLTVKIPLEKGI
>Mature_514_residues
SKGISTYSIRMRGARRRAGRALRRPQNTQAQLAGIIRSSMEAIISVDADQRVVLFNPMAETLFGWPADQAIGEPLTRFIP
ERFRAAHEAHVRRFGITGVSDRQMGRQRALYALRRDGTEFPIEASISQTTHRGSKLFTVMLRDITERTRAEKAMHASLQR
VRESEARLAGIIRSSMEAIITVDSTQHVILFNPMAETLFGWPADQAIGRPLGDFIPERFRAAHDAHVRRFGVTGVSDRQM
GRQRALYALRRDGSEFPIEASISQIADGGVSLYTVMLRDITERVRAEQALRRSREELQQLSDSILATREEEKRRIARELH
DDLGQRLSALKMDMTMLAADVSESRDIRGILADITDMNGVIDDTVASVRRIASDLRPALLDELGMLTAIEWLAADFSSRY
GLPVSVDGVDADVPEQTAIAMFRIVQEALSNVVRHADATVVGVSLAQADGQIELRVQDNGGGWDKKPAGTEPRKSLGLLG
IRERARLLGGAVEVDSEPGKGFRLTVKIPLEKGI

Specific function: Member of the two-component regulatory system degS/degU. Involved in a sensory transduction pathway that affect the production of enzymes that degrade polymeric carbon and nitrogen sources. DegS probably acts as a kinase that phosphorylates degU [H]

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=239, Percent_Identity=32.2175732217573, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI87082311, Length=246, Percent_Identity=28.4552845528455, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788812, Length=209, Percent_Identity=28.7081339712919, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87081921, Length=276, Percent_Identity=23.9130434782609, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR008595
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR016381 [H]

Pfam domain/function: PF05384 DegS; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 57136; Mature: 57005

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKGISTYSIRMRGARRRAGRALRRPQNTQAQLAGIIRSSMEAIISVDADQRVVLFNPMA
CCCCCCEEEEEECHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEECCCCCEEEEECCHH
ETLFGWPADQAIGEPLTRFIPERFRAAHEAHVRRFGITGVSDRQMGRQRALYALRRDGTE
HHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCC
FPIEASISQTTHRGSKLFTVMLRDITERTRAEKAMHASLQRVRESEARLAGIIRSSMEAI
CCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
ITVDSTQHVILFNPMAETLFGWPADQAIGRPLGDFIPERFRAAHDAHVRRFGVTGVSDRQ
EEECCCCEEEEECCHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
MGRQRALYALRRDGSEFPIEASISQIADGGVSLYTVMLRDITERVRAEQALRRSREELQQ
HHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSDSILATREEEKRRIARELHDDLGQRLSALKMDMTMLAADVSESRDIRGILADITDMNG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
VIDDTVASVRRIASDLRPALLDELGMLTAIEWLAADFSSRYGLPVSVDGVDADVPEQTAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHH
AMFRIVQEALSNVVRHADATVVGVSLAQADGQIELRVQDNGGGWDKKPAGTEPRKSLGLL
HHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCHHHHHCHH
GIRERARLLGGAVEVDSEPGKGFRLTVKIPLEKGI
HHHHHHHHHCCCEEECCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure 
SKGISTYSIRMRGARRRAGRALRRPQNTQAQLAGIIRSSMEAIISVDADQRVVLFNPMA
CCCCCEEEEEECHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEECCCCCEEEEECCHH
ETLFGWPADQAIGEPLTRFIPERFRAAHEAHVRRFGITGVSDRQMGRQRALYALRRDGTE
HHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCC
FPIEASISQTTHRGSKLFTVMLRDITERTRAEKAMHASLQRVRESEARLAGIIRSSMEAI
CCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
ITVDSTQHVILFNPMAETLFGWPADQAIGRPLGDFIPERFRAAHDAHVRRFGVTGVSDRQ
EEECCCCEEEEECCHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
MGRQRALYALRRDGSEFPIEASISQIADGGVSLYTVMLRDITERVRAEQALRRSREELQQ
HHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSDSILATREEEKRRIARELHDDLGQRLSALKMDMTMLAADVSESRDIRGILADITDMNG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
VIDDTVASVRRIASDLRPALLDELGMLTAIEWLAADFSSRYGLPVSVDGVDADVPEQTAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHH
AMFRIVQEALSNVVRHADATVVGVSLAQADGQIELRVQDNGGGWDKKPAGTEPRKSLGLL
HHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCHHHHHCHH
GIRERARLLGGAVEVDSEPGKGFRLTVKIPLEKGI
HHHHHHHHHCCCEEECCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3141378; 3141377; 9384377; 3136143 [H]