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The map label for this gene is gacA [H]

Identifier: 73540911

GI number: 73540911

Start: 1302224

End: 1302871

Strand: Direct

Name: gacA [H]

Synonym: Reut_A1216

Alternate gene names: 73540911

Gene position: 1302224-1302871 (Clockwise)

Preceding gene: 73540910

Following gene: 73540914

Centisome position: 34.21

GC content: 63.73

Gene sequence:

>648_bases
ATGACGCGCGTGCTCATTGCCGATGACCATGCCGTCGTGCGCGACGGTCTCAAGCATATCCTGGAGCGCGCCGGCAAATT
CGATGTGGTAGGCGAAGCCGCCGACGGCAGCCAGGTCCTGAAGCTCGTGCGCGACTGCGCGCCGCAGGTGCTGCTGCTCG
ACTTGTCGATGCCGGGCCGCAGCGGCCTGGAACTGATCCGCCTGTTGCGGGACGAGCAACCGGCGCTGCGCATCCTGGTA
CTGACCATGCATGCGGAGCAGCAATACATCGTGCGCGCGTTCCAGGCCGGTGCGGCGGGCTATCTGACCAAGGAAAGCGC
TGCGACCGAGCTGGTTTCTGCGATCACCCAGGTGGCAAGCGGGGGCACGTATGTCAGCCAGGCGATTGCCGGCAAGCTGG
CGACCAGCCTGCAGGAACATCAGGACGAAGCACCGCACTTGCGCCTGTCCGACCGCGAACTGGAAGTCTATCGGCGGCTG
GTGCTGGGCGAGCCACTCACCGCGATTGCGACAGCGCTGTGCGTGAGTGTCAAGACGGTCAGTACATACAAGATGCGGCT
GATGGAAAAGATGCACGTGCCCAGCGAAGCGGCGCTGCTGCGCTATGCCATGCGCCATCATCTGTTCGACGAGGAAGAGG
ACATCTGA

Upstream 100 bases:

>100_bases
GAGCGGGCGCGCTTGCTTGGCGGTGCCGTCGAAGTCGACAGCGAACCTGGCAAGGGATTCCGGCTGACCGTAAAGATTCC
TCTGGAAAAAGGGATATGAA

Downstream 100 bases:

>100_bases
GGCAGGCACGTGCCCCACGCAAGCGGCCGAGCCGCTTGAAGGTCAGGGGTTCGGATCCCTGGCCTTCCCCCGTAGCAAAC
TTCAGCTCGTTGACCAGCAT

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MTRVLIADDHAVVRDGLKHILERAGKFDVVGEAADGSQVLKLVRDCAPQVLLLDLSMPGRSGLELIRLLRDEQPALRILV
LTMHAEQQYIVRAFQAGAAGYLTKESAATELVSAITQVASGGTYVSQAIAGKLATSLQEHQDEAPHLRLSDRELEVYRRL
VLGEPLTAIATALCVSVKTVSTYKMRLMEKMHVPSEAALLRYAMRHHLFDEEEDI

Sequences:

>Translated_215_residues
MTRVLIADDHAVVRDGLKHILERAGKFDVVGEAADGSQVLKLVRDCAPQVLLLDLSMPGRSGLELIRLLRDEQPALRILV
LTMHAEQQYIVRAFQAGAAGYLTKESAATELVSAITQVASGGTYVSQAIAGKLATSLQEHQDEAPHLRLSDRELEVYRRL
VLGEPLTAIATALCVSVKTVSTYKMRLMEKMHVPSEAALLRYAMRHHLFDEEEDI
>Mature_214_residues
TRVLIADDHAVVRDGLKHILERAGKFDVVGEAADGSQVLKLVRDCAPQVLLLDLSMPGRSGLELIRLLRDEQPALRILVL
TMHAEQQYIVRAFQAGAAGYLTKESAATELVSAITQVASGGTYVSQAIAGKLATSLQEHQDEAPHLRLSDRELEVYRRLV
LGEPLTAIATALCVSVKTVSTYKMRLMEKMHVPSEAALLRYAMRHHLFDEEEDI

Specific function: Positively controls the production of the autoinducer N- butyryl-homoserine lactone and the formation of the virulence factors pyocyanine, cyanide, and lipase [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=213, Percent_Identity=38.9671361502347, Blast_Score=150, Evalue=4e-38,
Organism=Escherichia coli, GI1790102, Length=201, Percent_Identity=34.3283582089552, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788521, Length=196, Percent_Identity=33.6734693877551, Blast_Score=98, Evalue=3e-22,
Organism=Escherichia coli, GI1788546, Length=204, Percent_Identity=32.843137254902, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1788712, Length=204, Percent_Identity=26.9607843137255, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1787473, Length=182, Percent_Identity=34.0659340659341, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1786747, Length=207, Percent_Identity=26.0869565217391, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI87082052, Length=104, Percent_Identity=33.6538461538462, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1786599, Length=118, Percent_Identity=33.0508474576271, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1788191, Length=118, Percent_Identity=31.3559322033898, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1790299, Length=117, Percent_Identity=32.4786324786325, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23725; Mature: 23594

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRVLIADDHAVVRDGLKHILERAGKFDVVGEAADGSQVLKLVRDCAPQVLLLDLSMPGR
CCEEEEECCHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHCCCCEEEEEECCCCC
SGLELIRLLRDEQPALRILVLTMHAEQQYIVRAFQAGAAGYLTKESAATELVSAITQVAS
HHHHHHHHHHCCCCHHEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
GGTYVSQAIAGKLATSLQEHQDEAPHLRLSDRELEVYRRLVLGEPLTAIATALCVSVKTV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
STYKMRLMEKMHVPSEAALLRYAMRHHLFDEEEDI
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TRVLIADDHAVVRDGLKHILERAGKFDVVGEAADGSQVLKLVRDCAPQVLLLDLSMPGR
CEEEEECCHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHCCCCEEEEEECCCCC
SGLELIRLLRDEQPALRILVLTMHAEQQYIVRAFQAGAAGYLTKESAATELVSAITQVAS
HHHHHHHHHHCCCCHHEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
GGTYVSQAIAGKLATSLQEHQDEAPHLRLSDRELEVYRRLVLGEPLTAIATALCVSVKTV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
STYKMRLMEKMHVPSEAALLRYAMRHHLFDEEEDI
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9159518; 10984043 [H]