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The map label for this gene is lcfB [H]

Identifier: 73540891

GI number: 73540891

Start: 1278080

End: 1279627

Strand: Reverse

Name: lcfB [H]

Synonym: Reut_A1196

Alternate gene names: 73540891

Gene position: 1279627-1278080 (Counterclockwise)

Preceding gene: 73540892

Following gene: 73540890

Centisome position: 33.62

GC content: 63.44

Gene sequence:

>1548_bases
ATGCAGCCGATTGACTTCCTTCTGAGAACCGCAAGCGCGTTCCCCCACCGTATTGCTCTCGAAGCAGACGATGTCTCAAT
GACTTACGGCGAACTGGCCCGGCAGGTGTCCGCGCTGGCTGCCGCCTTCCAGGACATCGATCCGGTGCCAGGTTCGCGGG
TAGCCATCTGTGCCGGGAACAGCGTTCCGCACGTGGTCGCCTTAATGGCGGTCCTGGCGGCGGGAAAAACCTGGGTGCCG
CTCAATCCGCGCGGCGCCGGCCCGGAGCTTGCCAGGATAATCGACTTCACGACTCCATCGATTCTCGTGTTCGACGAAGC
TCACCGTGAACAGGCCTGTTACGCGGTCCCGGCCGGCGCGCGGCGCCTGGTTTGCGGCACCTCATCGGCTGAGCCGAACA
GTGTGGCGGGCCTGCTGGTGGCATGGCAAGGGCGTGTCCCGCGCCGCCAGTCAAGGGACCTGTCCGACATCCAGGCGATC
AAGTTCACTGGTGGTACGACCGGCGCCCCCAAAGGCGTCATGCAATCCTTGCGCGCCTGGAATGCCACGGTCGTATCGAT
CCTGACAGCAATGCAGTTGCAAGACGACGAGCGCTATCTCGCAGTGGCGTCTATCACGCACGGCACGTCGACATTTCTTC
TTCCCGTATTTGCCTGCGGGGGCACGATTGTCCTGGCGAGTTCGACCAGGCCGAAGTCAGTGCTGGACCAGCTGGAAAGC
GGCCGGATCACGGCGACCTTCATGCCGCCCACGCTGATCTACGACTTGCTCGATGAGCCCGGGGTGGCGACGCGAGACTG
GTCGCAGCTGCGGCATATGGTCTATGCCGCAGCGCCGATGCGGGCAGATCGTATTGCCCAGGCCATCGAGGTGTTTGGCA
CCCTCGAGACATCATTCGGACAGACGGAAGCGCCTGCGGTCATTGCATTCATGCCTTCGATCGAATTGCGCAATTCGGAA
CGCCATGGATCGGTCGGCCGCGCCACCCCGCTGACCGAGATTGGGATCATGGCGCCCGACGGCCGCTTGCTACCGCCGGG
AGAGACCGGCGAAATCGTCGTTCGCGGCGACCTGTTGATGAGCGGCTACTGGCGGCAGCCAGAAAAGACAGCCGAGACCA
TCGTCGACGGCTGGCTGCATACCGGCGATGGCGGGTACCTGGATCGGGAAGGCTTTCTCTACCTGAAGGACCGACTGCGG
GACGTTGTCATCAGCGGCGGCTTTAACGTCTATCCGACCGATGTCGAGAATGCTTTGGGCAAGCACCCCGCCGTCTGCCG
GTGCGCGGTCTTCGGCGTCGCCGATCCGCGCTGGGGAGAGGCGGTACATGCGGCCGTCCAACTCAGACCGGGCACAGAGG
TCTCGGCCGACACGCTCAGGGGTGTTGTCAGGGCACGACTTGGCCCCGTCCAGACGCCCAAGCAAATCCACTTTCTCGAC
AGCCTGCCCCTGACCGCGAACGGCAAGGTTTCAAAGCAGGCCCTCCGGGATCAACTGGCGAACTCGGACGGCAAGGCCCA
TTTCCCCAAGGAGACGCTCGCATCATGA

Upstream 100 bases:

>100_bases
GGCCCGCATCGTCAAACTGTCCGGGGCGCGACCAGATTGAAATCCATCACGCCACATCGTGCGGCAACCGTGTTGGCCGA
ATCTTCAAGCCGGAGTATTC

Downstream 100 bases:

>100_bases
TTCCAGAATCGGTTCTTGTCACCGGAGGCTCCGGCGGTATTGGCTGGGCCTTGTGCCAGACCCTGGCGAGACGCGGCAGC
CATGTCGTGAGCATCGACCG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MQPIDFLLRTASAFPHRIALEADDVSMTYGELARQVSALAAAFQDIDPVPGSRVAICAGNSVPHVVALMAVLAAGKTWVP
LNPRGAGPELARIIDFTTPSILVFDEAHREQACYAVPAGARRLVCGTSSAEPNSVAGLLVAWQGRVPRRQSRDLSDIQAI
KFTGGTTGAPKGVMQSLRAWNATVVSILTAMQLQDDERYLAVASITHGTSTFLLPVFACGGTIVLASSTRPKSVLDQLES
GRITATFMPPTLIYDLLDEPGVATRDWSQLRHMVYAAAPMRADRIAQAIEVFGTLETSFGQTEAPAVIAFMPSIELRNSE
RHGSVGRATPLTEIGIMAPDGRLLPPGETGEIVVRGDLLMSGYWRQPEKTAETIVDGWLHTGDGGYLDREGFLYLKDRLR
DVVISGGFNVYPTDVENALGKHPAVCRCAVFGVADPRWGEAVHAAVQLRPGTEVSADTLRGVVRARLGPVQTPKQIHFLD
SLPLTANGKVSKQALRDQLANSDGKAHFPKETLAS

Sequences:

>Translated_515_residues
MQPIDFLLRTASAFPHRIALEADDVSMTYGELARQVSALAAAFQDIDPVPGSRVAICAGNSVPHVVALMAVLAAGKTWVP
LNPRGAGPELARIIDFTTPSILVFDEAHREQACYAVPAGARRLVCGTSSAEPNSVAGLLVAWQGRVPRRQSRDLSDIQAI
KFTGGTTGAPKGVMQSLRAWNATVVSILTAMQLQDDERYLAVASITHGTSTFLLPVFACGGTIVLASSTRPKSVLDQLES
GRITATFMPPTLIYDLLDEPGVATRDWSQLRHMVYAAAPMRADRIAQAIEVFGTLETSFGQTEAPAVIAFMPSIELRNSE
RHGSVGRATPLTEIGIMAPDGRLLPPGETGEIVVRGDLLMSGYWRQPEKTAETIVDGWLHTGDGGYLDREGFLYLKDRLR
DVVISGGFNVYPTDVENALGKHPAVCRCAVFGVADPRWGEAVHAAVQLRPGTEVSADTLRGVVRARLGPVQTPKQIHFLD
SLPLTANGKVSKQALRDQLANSDGKAHFPKETLAS
>Mature_515_residues
MQPIDFLLRTASAFPHRIALEADDVSMTYGELARQVSALAAAFQDIDPVPGSRVAICAGNSVPHVVALMAVLAAGKTWVP
LNPRGAGPELARIIDFTTPSILVFDEAHREQACYAVPAGARRLVCGTSSAEPNSVAGLLVAWQGRVPRRQSRDLSDIQAI
KFTGGTTGAPKGVMQSLRAWNATVVSILTAMQLQDDERYLAVASITHGTSTFLLPVFACGGTIVLASSTRPKSVLDQLES
GRITATFMPPTLIYDLLDEPGVATRDWSQLRHMVYAAAPMRADRIAQAIEVFGTLETSFGQTEAPAVIAFMPSIELRNSE
RHGSVGRATPLTEIGIMAPDGRLLPPGETGEIVVRGDLLMSGYWRQPEKTAETIVDGWLHTGDGGYLDREGFLYLKDRLR
DVVISGGFNVYPTDVENALGKHPAVCRCAVFGVADPRWGEAVHAAVQLRPGTEVSADTLRGVVRARLGPVQTPKQIHFLD
SLPLTANGKVSKQALRDQLANSDGKAHFPKETLAS

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=541, Percent_Identity=28.4658040665434, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI42544132, Length=360, Percent_Identity=27.7777777777778, Blast_Score=136, Evalue=6e-32,
Organism=Homo sapiens, GI38505220, Length=366, Percent_Identity=29.2349726775956, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI187761345, Length=532, Percent_Identity=26.3157894736842, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI187761343, Length=532, Percent_Identity=26.3157894736842, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI122937307, Length=267, Percent_Identity=31.8352059925094, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI58082049, Length=364, Percent_Identity=28.5714285714286, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI157311624, Length=376, Percent_Identity=28.7234042553192, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI157311622, Length=376, Percent_Identity=28.7234042553192, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI115511026, Length=214, Percent_Identity=30.8411214953271, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI13375727, Length=515, Percent_Identity=25.8252427184466, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI28416953, Length=524, Percent_Identity=24.0458015267176, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI8923896, Length=389, Percent_Identity=25.4498714652956, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI253970489, Length=389, Percent_Identity=25.4498714652956, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI27477105, Length=196, Percent_Identity=29.5918367346939, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1788107, Length=509, Percent_Identity=28.2907662082515, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI221142682, Length=511, Percent_Identity=27.9843444227006, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI145693145, Length=519, Percent_Identity=26.0115606936416, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1786810, Length=518, Percent_Identity=24.9034749034749, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1790505, Length=532, Percent_Identity=24.812030075188, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788595, Length=482, Percent_Identity=24.896265560166, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789201, Length=351, Percent_Identity=27.3504273504274, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786801, Length=498, Percent_Identity=25.9036144578313, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17559526, Length=365, Percent_Identity=29.5890410958904, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI32563687, Length=502, Percent_Identity=26.8924302788845, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17558820, Length=489, Percent_Identity=26.9938650306748, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17560308, Length=532, Percent_Identity=26.8796992481203, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI71994690, Length=541, Percent_Identity=24.3992606284658, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71994703, Length=541, Percent_Identity=24.3992606284658, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71994694, Length=541, Percent_Identity=24.3992606284658, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71996755, Length=504, Percent_Identity=23.2142857142857, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17531443, Length=362, Percent_Identity=26.2430939226519, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17560140, Length=509, Percent_Identity=22.5933202357564, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17557194, Length=502, Percent_Identity=23.7051792828685, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17538037, Length=505, Percent_Identity=23.3663366336634, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71985884, Length=348, Percent_Identity=25.5747126436782, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17510401, Length=168, Percent_Identity=29.1666666666667, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17564090, Length=504, Percent_Identity=21.8253968253968, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=522, Percent_Identity=26.0536398467433, Blast_Score=147, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6319264, Length=387, Percent_Identity=26.3565891472868, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323182, Length=156, Percent_Identity=28.8461538461538, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24581924, Length=509, Percent_Identity=27.3084479371316, Blast_Score=162, Evalue=7e-40,
Organism=Drosophila melanogaster, GI21355181, Length=527, Percent_Identity=26.3757115749526, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI18859661, Length=481, Percent_Identity=25.7796257796258, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24648255, Length=503, Percent_Identity=25.2485089463221, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24648253, Length=489, Percent_Identity=25.3578732106339, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24648257, Length=461, Percent_Identity=27.9826464208243, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24648260, Length=349, Percent_Identity=29.2263610315186, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI21356947, Length=512, Percent_Identity=24.609375, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI20130357, Length=488, Percent_Identity=25.6147540983607, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI281365686, Length=366, Percent_Identity=24.0437158469945, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24653035, Length=454, Percent_Identity=24.8898678414097, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI21356441, Length=493, Percent_Identity=22.7180527383367, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161076582, Length=470, Percent_Identity=24.2553191489362, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24656500, Length=502, Percent_Identity=26.4940239043825, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI21358303, Length=498, Percent_Identity=23.2931726907631, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI19922652, Length=507, Percent_Identity=25.4437869822485, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24648676, Length=475, Percent_Identity=24.8421052631579, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19921316, Length=112, Percent_Identity=39.2857142857143, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 55286; Mature: 55286

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00690 DEAH_ATP_HELICASE ; PS00138 SUBTILASE_SER ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPIDFLLRTASAFPHRIALEADDVSMTYGELARQVSALAAAFQDIDPVPGSRVAICAGN
CCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
SVPHVVALMAVLAAGKTWVPLNPRGAGPELARIIDFTTPSILVFDEAHREQACYAVPAGA
CCHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHEECCCCCEEEEECCCCCCCEEECCCCC
RRLVCGTSSAEPNSVAGLLVAWQGRVPRRQSRDLSDIQAIKFTGGTTGAPKGVMQSLRAW
EEEEEECCCCCCCCCEEEEEEECCCCCCHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHC
NATVVSILTAMQLQDDERYLAVASITHGTSTFLLPVFACGGTIVLASSTRPKSVLDQLES
CHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHC
GRITATFMPPTLIYDLLDEPGVATRDWSQLRHMVYAAAPMRADRIAQAIEVFGTLETSFG
CCEEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
QTEAPAVIAFMPSIELRNSERHGSVGRATPLTEIGIMAPDGRLLPPGETGEIVVRGDLLM
CCCCCEEEEEECCCEECCCCCCCCCCCCCCCHHEEEECCCCCCCCCCCCCCEEEECCEEE
SGYWRQPEKTAETIVDGWLHTGDGGYLDREGFLYLKDRLRDVVISGGFNVYPTDVENALG
CCCCCCCHHHHHHHHHCCEECCCCCEECCCCEEHHHHHHHHEEEECCCEECCCCHHHHCC
KHPAVCRCAVFGVADPRWGEAVHAAVQLRPGTEVSADTLRGVVRARLGPVQTPKQIHFLD
CCCCEEEEEEEECCCCCCCHHEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEC
SLPLTANGKVSKQALRDQLANSDGKAHFPKETLAS
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHCC
>Mature Secondary Structure
MQPIDFLLRTASAFPHRIALEADDVSMTYGELARQVSALAAAFQDIDPVPGSRVAICAGN
CCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
SVPHVVALMAVLAAGKTWVPLNPRGAGPELARIIDFTTPSILVFDEAHREQACYAVPAGA
CCHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHEECCCCCEEEEECCCCCCCEEECCCCC
RRLVCGTSSAEPNSVAGLLVAWQGRVPRRQSRDLSDIQAIKFTGGTTGAPKGVMQSLRAW
EEEEEECCCCCCCCCEEEEEEECCCCCCHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHC
NATVVSILTAMQLQDDERYLAVASITHGTSTFLLPVFACGGTIVLASSTRPKSVLDQLES
CHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHC
GRITATFMPPTLIYDLLDEPGVATRDWSQLRHMVYAAAPMRADRIAQAIEVFGTLETSFG
CCEEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
QTEAPAVIAFMPSIELRNSERHGSVGRATPLTEIGIMAPDGRLLPPGETGEIVVRGDLLM
CCCCCEEEEEECCCEECCCCCCCCCCCCCCCHHEEEECCCCCCCCCCCCCCEEEECCEEE
SGYWRQPEKTAETIVDGWLHTGDGGYLDREGFLYLKDRLRDVVISGGFNVYPTDVENALG
CCCCCCCHHHHHHHHHCCEECCCCCEECCCCEEHHHHHHHHEEEECCCEECCCCHHHHCC
KHPAVCRCAVFGVADPRWGEAVHAAVQLRPGTEVSADTLRGVVRARLGPVQTPKQIHFLD
CCCCEEEEEEEECCCCCCCHHEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEC
SLPLTANGKVSKQALRDQLANSDGKAHFPKETLAS
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]