Click here to switch to the map view.

The map label for this gene is tilS [H]

Identifier: 73540827

GI number: 73540827

Start: 1217181

End: 1218635

Strand: Direct

Name: tilS [H]

Synonym: Reut_A1125

Alternate gene names: 73540827

Gene position: 1217181-1218635 (Clockwise)

Preceding gene: 73540826

Following gene: 73540828

Centisome position: 31.98

GC content: 69.21

Gene sequence:

>1455_bases
ATGGCAAGTTCAAGGAAACCGGCGCCCAGGAGTGATCCGTCCGATCGCCTGACCGACAAGGTCGCACAGGCTCTTCAGGC
CAGTGCGGCCTTTGTTGTTTCTGGCGGGACGGGCGCAAGCGCTACCGACATGGTGGTGGCCGTGGCGCTGTCCGGCGGCC
GCGACTCCGTAGCGCTTCTCCATGCCACGCAGGCGGCCGTGTCTGCGTGGCGCATGCCGGCCCGCGTGGTCGCCCTGCAT
GTTCACCATGGCCTGCAGGCGCAGGCCGATGCGTGGGATGCATTCTGCGCGGATCTGTGCGCGCGATGGGAGCTGGGTTA
TTTCGTGCGCCGCGTGTCAGTGCAGGCCGGCACGGGCGAGGGCATCGAGGCCGCCGCGAGGCGCGCGCGCTATGAAGCGC
TCGGCGACATGTGCCGAGCGAGCGGTGCCCGCTTTTTGCTGTTCGCGCACCATCTCGATGACCAGGTCGAAACCGTGCTG
CTCCGTCTCTTTCGCGGCGCGGGAGTGGCGGGTATGTCCGGCATGCCTGCGATGCGCAAACTGGACGAAGCGGGCGACAT
CATGCTTTTGCGGCCATGGCTCGAAGTCCCGCGTGCCGACATCGACAGCTATTGCGCCGCGCATGAACTCGCATGGATCG
ATGATCCGTCCAACGACAGCACGCGTTATGCGCGCAACGCCTTGCGCGCGCAATTGCCGCTGCTGGAGCGGGATTTCCCC
GCCTTGCGCGCCAATGTTGCCCAGGCCGCCGCGCACTTTGCGCAGGCCGCCCGCATGCTCGACGATCTCGCGGGCACCGA
GCTGGCTCGGCTGGCCGCGCCAGGACGCGATCCCGATACGCTCGCCGAACTGGACCTGTCAGGCCTGCGCGCGCTACCCG
CTTCCCACGCCGATGCCGTGCTGCGTCTCTGGTTACGCGACCTCGGCGCACGCGCGCCATCCACGGCGAGGCTGGCGGCT
ATGCGCGAACAGTTGCTTGACCACAGTGGTGGTGAACCGGCCATCGCCCATGACAACCTGGTGCTGCGCCGATTCCAGGG
CAGGGTGCTGGCCTGCACGCCGGAGCCGGATGCGCCGCCGACGGCGCAGGCGCTGACCTGGCGCGGCGAGCCGCGCATCG
TAGTCCCTGCCTGGCGTGGCGAACTGCGCTTTACGCGCGACGATACCTTCGGCGTGCCGGAAGCCGTGCTCCAGCAGCCG
TTGCGGATCGCGCAGCGCACTGGCGGCGAGCGCATCGTGCTGCGGCCCGGCGGCCCCGCGCGCGCACTCAAGCAGGCTTA
TCAGGAGGCCGGTGTGCCGCGCTGGCGCCGATGCTGGCTGCCACTGCTGTGGGCCGGCGATACGCTTGTGATGGCCGCAG
GCCTGGGCATGCACCGGCGCTGGACAGAGGCGCCGCACGCGCCGCGCTGGCGCGTCGAGTGGATGCAGGCGACGGCTGGT
ACACGCATCCCGTAG

Upstream 100 bases:

>100_bases
GCCGCCATGCTCAAGCGTTCGCTGGCTGAATCCCTGCGCCAGTTCCAGGGCGTGAGCGTGAAGGAACTGCAGGCGCGCCG
CTACGAGCGCCTGCTGGCCT

Downstream 100 bases:

>100_bases
CCAGGATTTTCGACGCCAACATCACATTCCATCCGCCGGCGAAGCCGCTACGCCTTGCCTGATGGTCGCGGTTCGTGTAT
TTTCAAGAGCTTTGCACAAT

Product: PP-loop

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MASSRKPAPRSDPSDRLTDKVAQALQASAAFVVSGGTGASATDMVVAVALSGGRDSVALLHATQAAVSAWRMPARVVALH
VHHGLQAQADAWDAFCADLCARWELGYFVRRVSVQAGTGEGIEAAARRARYEALGDMCRASGARFLLFAHHLDDQVETVL
LRLFRGAGVAGMSGMPAMRKLDEAGDIMLLRPWLEVPRADIDSYCAAHELAWIDDPSNDSTRYARNALRAQLPLLERDFP
ALRANVAQAAAHFAQAARMLDDLAGTELARLAAPGRDPDTLAELDLSGLRALPASHADAVLRLWLRDLGARAPSTARLAA
MREQLLDHSGGEPAIAHDNLVLRRFQGRVLACTPEPDAPPTAQALTWRGEPRIVVPAWRGELRFTRDDTFGVPEAVLQQP
LRIAQRTGGERIVLRPGGPARALKQAYQEAGVPRWRRCWLPLLWAGDTLVMAAGLGMHRRWTEAPHAPRWRVEWMQATAG
TRIP

Sequences:

>Translated_484_residues
MASSRKPAPRSDPSDRLTDKVAQALQASAAFVVSGGTGASATDMVVAVALSGGRDSVALLHATQAAVSAWRMPARVVALH
VHHGLQAQADAWDAFCADLCARWELGYFVRRVSVQAGTGEGIEAAARRARYEALGDMCRASGARFLLFAHHLDDQVETVL
LRLFRGAGVAGMSGMPAMRKLDEAGDIMLLRPWLEVPRADIDSYCAAHELAWIDDPSNDSTRYARNALRAQLPLLERDFP
ALRANVAQAAAHFAQAARMLDDLAGTELARLAAPGRDPDTLAELDLSGLRALPASHADAVLRLWLRDLGARAPSTARLAA
MREQLLDHSGGEPAIAHDNLVLRRFQGRVLACTPEPDAPPTAQALTWRGEPRIVVPAWRGELRFTRDDTFGVPEAVLQQP
LRIAQRTGGERIVLRPGGPARALKQAYQEAGVPRWRRCWLPLLWAGDTLVMAAGLGMHRRWTEAPHAPRWRVEWMQATAG
TRIP
>Mature_483_residues
ASSRKPAPRSDPSDRLTDKVAQALQASAAFVVSGGTGASATDMVVAVALSGGRDSVALLHATQAAVSAWRMPARVVALHV
HHGLQAQADAWDAFCADLCARWELGYFVRRVSVQAGTGEGIEAAARRARYEALGDMCRASGARFLLFAHHLDDQVETVLL
RLFRGAGVAGMSGMPAMRKLDEAGDIMLLRPWLEVPRADIDSYCAAHELAWIDDPSNDSTRYARNALRAQLPLLERDFPA
LRANVAQAAAHFAQAARMLDDLAGTELARLAAPGRDPDTLAELDLSGLRALPASHADAVLRLWLRDLGARAPSTARLAAM
REQLLDHSGGEPAIAHDNLVLRRFQGRVLACTPEPDAPPTAQALTWRGEPRIVVPAWRGELRFTRDDTFGVPEAVLQQPL
RIAQRTGGERIVLRPGGPARALKQAYQEAGVPRWRRCWLPLLWAGDTLVMAAGLGMHRRWTEAPHAPRWRVEWMQATAGT
RIP

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=424, Percent_Identity=35.377358490566, Blast_Score=165, Evalue=6e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 52644; Mature: 52513

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSRKPAPRSDPSDRLTDKVAQALQASAAFVVSGGTGASATDMVVAVALSGGRDSVALL
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCHHHEEEEEEECCCCCCEEEH
HATQAAVSAWRMPARVVALHVHHGLQAQADAWDAFCADLCARWELGYFVRRVSVQAGTGE
HHHHHHHHHHCCCHHHEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCC
GIEAAARRARYEALGDMCRASGARFLLFAHHLDDQVETVLLRLFRGAGVAGMSGMPAMRK
CHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHH
LDEAGDIMLLRPWLEVPRADIDSYCAAHELAWIDDPSNDSTRYARNALRAQLPLLERDFP
HHCCCCEEEECCHHHCCCCCHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHCCHHHCCCH
ALRANVAQAAAHFAQAARMLDDLAGTELARLAAPGRDPDTLAELDLSGLRALPASHADAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHHHHHHCCCHHHHHH
LRLWLRDLGARAPSTARLAAMREQLLDHSGGEPAIAHDNLVLRRFQGRVLACTPEPDAPP
HHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHCCCEEEECCCCCCCC
TAQALTWRGEPRIVVPAWRGELRFTRDDTFGVPEAVLQQPLRIAQRTGGERIVLRPGGPA
CCCEEEECCCCCEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHH
RALKQAYQEAGVPRWRRCWLPLLWAGDTLVMAAGLGMHRRWTEAPHAPRWRVEWMQATAG
HHHHHHHHHCCCCHHHHHCCHHEECCCHHHHHHCCCCCCHHCCCCCCCCHHHHHHHHCCC
TRIP
CCCC
>Mature Secondary Structure 
ASSRKPAPRSDPSDRLTDKVAQALQASAAFVVSGGTGASATDMVVAVALSGGRDSVALL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCHHHEEEEEEECCCCCCEEEH
HATQAAVSAWRMPARVVALHVHHGLQAQADAWDAFCADLCARWELGYFVRRVSVQAGTGE
HHHHHHHHHHCCCHHHEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCC
GIEAAARRARYEALGDMCRASGARFLLFAHHLDDQVETVLLRLFRGAGVAGMSGMPAMRK
CHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHH
LDEAGDIMLLRPWLEVPRADIDSYCAAHELAWIDDPSNDSTRYARNALRAQLPLLERDFP
HHCCCCEEEECCHHHCCCCCHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHCCHHHCCCH
ALRANVAQAAAHFAQAARMLDDLAGTELARLAAPGRDPDTLAELDLSGLRALPASHADAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHHHHHHCCCHHHHHH
LRLWLRDLGARAPSTARLAAMREQLLDHSGGEPAIAHDNLVLRRFQGRVLACTPEPDAPP
HHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHCCCEEEECCCCCCCC
TAQALTWRGEPRIVVPAWRGELRFTRDDTFGVPEAVLQQPLRIAQRTGGERIVLRPGGPA
CCCEEEECCCCCEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHH
RALKQAYQEAGVPRWRRCWLPLLWAGDTLVMAAGLGMHRRWTEAPHAPRWRVEWMQATAG
HHHHHHHHHCCCCHHHHHCCHHEECCCHHHHHHCCCCCCHHCCCCCCCCHHHHHHHHCCC
TRIP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]