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The map label for this gene is yafV [C]

Identifier: 73540731

GI number: 73540731

Start: 1117026

End: 1117847

Strand: Reverse

Name: yafV [C]

Synonym: Reut_A1028

Alternate gene names: 73540731

Gene position: 1117847-1117026 (Counterclockwise)

Preceding gene: 73540732

Following gene: 73540730

Centisome position: 29.37

GC content: 66.79

Gene sequence:

>822_bases
ATGCAAGCCCCTGCCCCGTTCCGTGTTGCCGCCATCCAGACCGTGACCGGCACGTCGCTCGACGCCAACCTCGCACGTGC
TGAATCGCTGATTGCCGAAGCCGCGCGCGGCGGCGCGGAACTGGTGCTGTTGCCCGAATACTTCTGCATGATGGGCCAGC
GCGAATCCGACAAGATCGCCATCCGCGAACAGGACGGCGACGGCCCCGTGCAACGGTTCCTGGCCGATGCCGCGAAGCGC
CACCGCGTCTGGCTGGTGGGGGGTACCCTGCCGATGTGGTGCGGCGACGACGACCGCGTCTACAACACGTCGCTCGCGTT
CGACCCGCGCGGCGAACGCGTGGCGCGCTACGACAAAATCCACCTGTTCGGCTTTACGCGCGGCGCGGAAAGCTATGACG
AATCGCGCACCATCCTCGCGGGCAACACGCCCGTCAGCTTCGACGCGCCATGTGGACGCGTAGCAATGTCGGTGTGCTAC
GACCTGCGCTTCCCTGAACTCTATCGCGGTCTGGCCGGCGGCGACGGCGCGAGCCTGATCCTGATGCCCGCCGCCTTCAC
CTACACCACCGGACAGGCACACTGGGAAATCCTGTTGCGTGCGCGTGCGATCGAAAACCAGTGCTATGTGCTTGCCGCGG
CACAGGGCGGCAAGCACGAGAATGGCCGGCGTACCTGGGGCCATTCGATGCTGGTCGACCCATGGGGCGAAGTGCTGGCC
ATGCTGCCCGAAGGCGAAGGCGTGGTCAGCGGCGTGATCGACCCGGCGCGGCTAGCCGAAGTCCGACAGAACCTGCCCGC
GCTGCGACACCGGGTGCTGTAG

Upstream 100 bases:

>100_bases
GGGGACAATAAGCCGCAACATCCGACGTACCAGAACCGCGCCGAGCCGGCCCTGCAGCGCTGATCCCCGCGCCGCGGCGC
GCTGCCAGAGGAAACGACCC

Downstream 100 bases:

>100_bases
CATTGGCGGTAACTCCGCCTTCCAACCTGATTCACGACGCCGGCGACCATGTCGGCGCATCTGCAAGAAAGAAAAGGACT
GTTCATGAACGCCGGCGATC

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: carboxylate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MQAPAPFRVAAIQTVTGTSLDANLARAESLIAEAARGGAELVLLPEYFCMMGQRESDKIAIREQDGDGPVQRFLADAAKR
HRVWLVGGTLPMWCGDDDRVYNTSLAFDPRGERVARYDKIHLFGFTRGAESYDESRTILAGNTPVSFDAPCGRVAMSVCY
DLRFPELYRGLAGGDGASLILMPAAFTYTTGQAHWEILLRARAIENQCYVLAAAQGGKHENGRRTWGHSMLVDPWGEVLA
MLPEGEGVVSGVIDPARLAEVRQNLPALRHRVL

Sequences:

>Translated_273_residues
MQAPAPFRVAAIQTVTGTSLDANLARAESLIAEAARGGAELVLLPEYFCMMGQRESDKIAIREQDGDGPVQRFLADAAKR
HRVWLVGGTLPMWCGDDDRVYNTSLAFDPRGERVARYDKIHLFGFTRGAESYDESRTILAGNTPVSFDAPCGRVAMSVCY
DLRFPELYRGLAGGDGASLILMPAAFTYTTGQAHWEILLRARAIENQCYVLAAAQGGKHENGRRTWGHSMLVDPWGEVLA
MLPEGEGVVSGVIDPARLAEVRQNLPALRHRVL
>Mature_273_residues
MQAPAPFRVAAIQTVTGTSLDANLARAESLIAEAARGGAELVLLPEYFCMMGQRESDKIAIREQDGDGPVQRFLADAAKR
HRVWLVGGTLPMWCGDDDRVYNTSLAFDPRGERVARYDKIHLFGFTRGAESYDESRTILAGNTPVSFDAPCGRVAMSVCY
DLRFPELYRGLAGGDGASLILMPAAFTYTTGQAHWEILLRARAIENQCYVLAAAQGGKHENGRRTWGHSMLVDPWGEVLA
MLPEGEGVVSGVIDPARLAEVRQNLPALRHRVL

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=266, Percent_Identity=38.7218045112782, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI5031947, Length=270, Percent_Identity=38.5185185185185, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI297632350, Length=270, Percent_Identity=38.5185185185185, Blast_Score=172, Evalue=2e-43,
Organism=Homo sapiens, GI297632348, Length=270, Percent_Identity=38.5185185185185, Blast_Score=172, Evalue=2e-43,
Organism=Homo sapiens, GI297632346, Length=195, Percent_Identity=32.3076923076923, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI1786412, Length=232, Percent_Identity=30.6034482758621, Blast_Score=76, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556280, Length=266, Percent_Identity=38.3458646616541, Blast_Score=175, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6323383, Length=274, Percent_Identity=33.9416058394161, Blast_Score=166, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6322335, Length=296, Percent_Identity=32.0945945945946, Blast_Score=143, Evalue=2e-35,
Organism=Drosophila melanogaster, GI17933642, Length=258, Percent_Identity=39.1472868217054, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI21355835, Length=270, Percent_Identity=34.4444444444444, Blast_Score=157, Evalue=7e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.5.1

Molecular weight: Translated: 29888; Mature: 29888

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAPAPFRVAAIQTVTGTSLDANLARAESLIAEAARGGAELVLLPEYFCMMGQRESDKIA
CCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHCCCCCCCEEE
IREQDGDGPVQRFLADAAKRHRVWLVGGTLPMWCGDDDRVYNTSLAFDPRGERVARYDKI
EEECCCCCHHHHHHHHHHHHCEEEEEECCEEEEECCCCCEEEEEEEECCCCCCHHHEEEE
HLFGFTRGAESYDESRTILAGNTPVSFDAPCGRVAMSVCYDLRFPELYRGLAGGDGASLI
EEEEEECCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCEEE
LMPAAFTYTTGQAHWEILLRARAIENQCYVLAAAQGGKHENGRRTWGHSMLVDPWGEVLA
EEEEEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEECCHHHHEE
MLPEGEGVVSGVIDPARLAEVRQNLPALRHRVL
ECCCCCCEEECCCCHHHHHHHHHCCHHHHHHCC
>Mature Secondary Structure
MQAPAPFRVAAIQTVTGTSLDANLARAESLIAEAARGGAELVLLPEYFCMMGQRESDKIA
CCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHCCCCCCCEEE
IREQDGDGPVQRFLADAAKRHRVWLVGGTLPMWCGDDDRVYNTSLAFDPRGERVARYDKI
EEECCCCCHHHHHHHHHHHHCEEEEEECCEEEEECCCCCEEEEEEEECCCCCCHHHEEEE
HLFGFTRGAESYDESRTILAGNTPVSFDAPCGRVAMSVCYDLRFPELYRGLAGGDGASLI
EEEEEECCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCEEE
LMPAAFTYTTGQAHWEILLRARAIENQCYVLAAAQGGKHENGRRTWGHSMLVDPWGEVLA
EEEEEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEECCHHHHEE
MLPEGEGVVSGVIDPARLAEVRQNLPALRHRVL
ECCCCCCEEECCCCHHHHHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrile; H2O

Specific reaction: A nitrile + H2O = a carboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]