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The map label for this gene is folA [H]

Identifier: 73540617

GI number: 73540617

Start: 994661

End: 995161

Strand: Reverse

Name: folA [H]

Synonym: Reut_A0914

Alternate gene names: 73540617

Gene position: 995161-994661 (Counterclockwise)

Preceding gene: 161611264

Following gene: 73540616

Centisome position: 26.14

GC content: 59.88

Gene sequence:

>501_bases
ATGACGTTGCTGACTCTCGTCGTCGCACGTGCCCGCAATGGCACCATCGGCCGCAACAACACGCTGCCCTGGCGGTTGCC
CGAAGACCTCGCGCATTTCAAGCGGACTACCATGGGCGCGCCGATCATCATGGGTCGCAAGACCTGGGATTCGATTGGCC
GGCCATTGCCTGGGCGGCGCAATATTGTCGTAAGCCGCAACGCGGATCTGAAACTGCAAGGCGCGGAAGTTGCCACCTCG
CTGGAAGATGCGCAAAGGCTCTGCGTAGGCGTCGAACAGGTGTTTCTGATCGGCGGTGCGCAGCTCTATGCCGAAGCGCT
GCCCAGTGCGGATCGGCTAGTCGTGACGGAGATTGATGCGGATGTAGAGGGCGATGCCTTCTTCCCCGCCATCGATCAGG
CGAAATGGATCGAGACGTCGCGTGAAACGCATCATTCGGAAGCCAATGGCTTTGACTATGCGTTCGTGACGTATGATCGG
CCGCCTTCCGGGGAAGAGTAA

Upstream 100 bases:

>100_bases
AGATCGTGCGCAAGCCGGACAGCATCTTCGACTACCGCTACGAAGATTTCGAGCTGGTCGGCTACCAGTCGCATCCCGCC
ATCAAGGCACCGGTGGCCGT

Downstream 100 bases:

>100_bases
AAAAGGCCGGCCAGGCTCAATACGAAAGCAGGTGCGATCCGCACCTGCTTTTTTTTCGCCTATGCCTCCAACGTGTTTTC
GAGCGGCTGGAGCACGATGT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MTLLTLVVARARNGTIGRNNTLPWRLPEDLAHFKRTTMGAPIIMGRKTWDSIGRPLPGRRNIVVSRNADLKLQGAEVATS
LEDAQRLCVGVEQVFLIGGAQLYAEALPSADRLVVTEIDADVEGDAFFPAIDQAKWIETSRETHHSEANGFDYAFVTYDR
PPSGEE

Sequences:

>Translated_166_residues
MTLLTLVVARARNGTIGRNNTLPWRLPEDLAHFKRTTMGAPIIMGRKTWDSIGRPLPGRRNIVVSRNADLKLQGAEVATS
LEDAQRLCVGVEQVFLIGGAQLYAEALPSADRLVVTEIDADVEGDAFFPAIDQAKWIETSRETHHSEANGFDYAFVTYDR
PPSGEE
>Mature_165_residues
TLLTLVVARARNGTIGRNNTLPWRLPEDLAHFKRTTMGAPIIMGRKTWDSIGRPLPGRRNIVVSRNADLKLQGAEVATSL
EDAQRLCVGVEQVFLIGGAQLYAEALPSADRLVVTEIDADVEGDAFFPAIDQAKWIETSRETHHSEANGFDYAFVTYDRP
PSGEE

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=181, Percent_Identity=35.9116022099448, Blast_Score=93, Evalue=1e-19,
Organism=Homo sapiens, GI28827793, Length=155, Percent_Identity=38.0645161290323, Blast_Score=80, Evalue=8e-16,
Organism=Escherichia coli, GI1786233, Length=159, Percent_Identity=42.1383647798742, Blast_Score=134, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17505899, Length=185, Percent_Identity=32.4324324324324, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6324810, Length=124, Percent_Identity=29.8387096774194, Blast_Score=66, Evalue=3e-12,
Organism=Drosophila melanogaster, GI116008026, Length=149, Percent_Identity=36.241610738255, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24647458, Length=149, Percent_Identity=36.241610738255, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18321; Mature: 18190

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLTLVVARARNGTIGRNNTLPWRLPEDLAHFKRTTMGAPIIMGRKTWDSIGRPLPGRR
CHHHHEEHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCHHHHCCCCCCCC
NIVVSRNADLKLQGAEVATSLEDAQRLCVGVEQVFLIGGAQLYAEALPSADRLVVTEIDA
CEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEEECC
DVEGDAFFPAIDQAKWIETSRETHHSEANGFDYAFVTYDRPPSGEE
CCCCCEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure 
TLLTLVVARARNGTIGRNNTLPWRLPEDLAHFKRTTMGAPIIMGRKTWDSIGRPLPGRR
HHHHEEHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCHHHHCCCCCCCC
NIVVSRNADLKLQGAEVATSLEDAQRLCVGVEQVFLIGGAQLYAEALPSADRLVVTEIDA
CEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEEECC
DVEGDAFFPAIDQAKWIETSRETHHSEANGFDYAFVTYDRPPSGEE
CCCCCEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6434541 [H]