Click here to switch to the map view.

The map label for this gene is thyA [H]

Identifier: 161611264

GI number: 161611264

Start: 995158

End: 995952

Strand: Reverse

Name: thyA [H]

Synonym: Reut_A0915

Alternate gene names: 161611264

Gene position: 995952-995158 (Counterclockwise)

Preceding gene: 73540619

Following gene: 73540617

Centisome position: 26.16

GC content: 63.02

Gene sequence:

>795_bases
ATGAAACAGTACCTCGACTTCATGCGCCATGTGTTCGAGCATGGCACCGAAAAGGCCGACCGCACCGGCACCGGCACGCG
CTCGGTCTTCGGCTACCAGATGCGCTTCGACCTGAGCCAGGGCTTCCCGGTGGTCACCACCAAGAAGCTGCACCTGAAGT
CGATCATCCTCGAACTGCTGTGGTTCCTGCAAGGCTCGACCAATGTGCGCTGGCTGCAGGAGAACGGCGTCAGCATCTGG
GACGAATGGGCCGACGAGAACGGCGAACTGGGCCCGGTCTACGGCTCCCAGTGGCGTTCGTGGCCGACGCCCGACGGCCG
CCATGTCGACCAGATCACCGACCTGCTGAACCAGATCCGCGAGAACCCCGATTCGCGCCGCCTGATTGTCTCGGCCTGGA
ACGTGGCCGACATCCCGCGCATGAAGCTGCCGCCGTGCCATGCGTTCTTCCAGTTCTACGTGGCCGATGGGCGTCTGTCG
TGCCAACTCTATCAGCGCAGCGCCGACATCTTCCTCGGCGTGCCGTTCAACATCGCCAGCTACGCGCTACTCACGCACAT
GATCGCGCAGCAGACCGGGCTGGACGTCGGCGATTTCGTCTGGACCGGCGGCGATTGCCATATCTACAGCAACCACTACG
AGCAGGTGCAGACGCAGCTGTCGCGTGAGCCGCTGCCACTGCCGACGCTGAAGATCGTGCGCAAGCCGGACAGCATCTTC
GACTACCGCTACGAAGATTTCGAGCTGGTCGGCTACCAGTCGCATCCCGCCATCAAGGCACCGGTGGCCGTATGA

Upstream 100 bases:

>100_bases
GGCCACTGCCGGAAGGGCCGCCCGAAGGGGTGGCCCTTCTTGCTTTAAAATGTCCGGTTTGCGGGCCAGCGCCCGCGTCA
CTTCCCATCCCGCCGCGCCG

Downstream 100 bases:

>100_bases
CGTTGCTGACTCTCGTCGTCGCACGTGCCCGCAATGGCACCATCGGCCGCAACAACACGCTGCCCTGGCGGTTGCCCGAA
GACCTCGCGCATTTCAAGCG

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKQYLDFMRHVFEHGTEKADRTGTGTRSVFGYQMRFDLSQGFPVVTTKKLHLKSIILELLWFLQGSTNVRWLQENGVSIW
DEWADENGELGPVYGSQWRSWPTPDGRHVDQITDLLNQIRENPDSRRLIVSAWNVADIPRMKLPPCHAFFQFYVADGRLS
CQLYQRSADIFLGVPFNIASYALLTHMIAQQTGLDVGDFVWTGGDCHIYSNHYEQVQTQLSREPLPLPTLKIVRKPDSIF
DYRYEDFELVGYQSHPAIKAPVAV

Sequences:

>Translated_264_residues
MKQYLDFMRHVFEHGTEKADRTGTGTRSVFGYQMRFDLSQGFPVVTTKKLHLKSIILELLWFLQGSTNVRWLQENGVSIW
DEWADENGELGPVYGSQWRSWPTPDGRHVDQITDLLNQIRENPDSRRLIVSAWNVADIPRMKLPPCHAFFQFYVADGRLS
CQLYQRSADIFLGVPFNIASYALLTHMIAQQTGLDVGDFVWTGGDCHIYSNHYEQVQTQLSREPLPLPTLKIVRKPDSIF
DYRYEDFELVGYQSHPAIKAPVAV
>Mature_264_residues
MKQYLDFMRHVFEHGTEKADRTGTGTRSVFGYQMRFDLSQGFPVVTTKKLHLKSIILELLWFLQGSTNVRWLQENGVSIW
DEWADENGELGPVYGSQWRSWPTPDGRHVDQITDLLNQIRENPDSRRLIVSAWNVADIPRMKLPPCHAFFQFYVADGRLS
CQLYQRSADIFLGVPFNIASYALLTHMIAQQTGLDVGDFVWTGGDCHIYSNHYEQVQTQLSREPLPLPTLKIVRKPDSIF
DYRYEDFELVGYQSHPAIKAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=282, Percent_Identity=51.4184397163121, Blast_Score=290, Evalue=1e-78,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=72.3484848484848, Blast_Score=425, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI71993377, Length=283, Percent_Identity=46.2897526501767, Blast_Score=260, Evalue=6e-70,
Organism=Saccharomyces cerevisiae, GI6324648, Length=228, Percent_Identity=44.2982456140351, Blast_Score=190, Evalue=2e-49,
Organism=Drosophila melanogaster, GI17137556, Length=284, Percent_Identity=51.4084507042254, Blast_Score=289, Evalue=1e-78,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 30441; Mature: 30441

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLDFMRHVFEHGTEKADRTGTGTRSVFGYQMRFDLSQGFPVVTTKKLHLKSIILELL
CHHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEEEEECCCCCCEEEEHHHHHHHHHHHHH
WFLQGSTNVRWLQENGVSIWDEWADENGELGPVYGSQWRSWPTPDGRHVDQITDLLNQIR
HHHCCCCCEEEEECCCCEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ENPDSRRLIVSAWNVADIPRMKLPPCHAFFQFYVADGRLSCQLYQRSADIFLGVPFNIAS
CCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCEEEECCHHHHH
YALLTHMIAQQTGLDVGDFVWTGGDCHIYSNHYEQVQTQLSREPLPLPTLKIVRKPDSIF
HHHHHHHHHHHHCCCCHHEEEECCCEEEECCHHHHHHHHHCCCCCCCCHHHEEECCCHHH
DYRYEDFELVGYQSHPAIKAPVAV
CCCCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MKQYLDFMRHVFEHGTEKADRTGTGTRSVFGYQMRFDLSQGFPVVTTKKLHLKSIILELL
CHHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEEEEECCCCCCEEEEHHHHHHHHHHHHH
WFLQGSTNVRWLQENGVSIWDEWADENGELGPVYGSQWRSWPTPDGRHVDQITDLLNQIR
HHHCCCCCEEEEECCCCEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ENPDSRRLIVSAWNVADIPRMKLPPCHAFFQFYVADGRLSCQLYQRSADIFLGVPFNIAS
CCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCEEEECCHHHHH
YALLTHMIAQQTGLDVGDFVWTGGDCHIYSNHYEQVQTQLSREPLPLPTLKIVRKPDSIF
HHHHHHHHHHHHCCCCHHEEEECCCEEEECCHHHHHHHHHCCCCCCCCHHHEEECCCHHH
DYRYEDFELVGYQSHPAIKAPVAV
CCCCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA