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The map label for this gene is glyA

Identifier: 73540494

GI number: 73540494

Start: 860139

End: 861386

Strand: Reverse

Name: glyA

Synonym: Reut_A0790

Alternate gene names: 73540494

Gene position: 861386-860139 (Counterclockwise)

Preceding gene: 73540505

Following gene: 73540493

Centisome position: 22.63

GC content: 63.78

Gene sequence:

>1248_bases
ATGTTCGAACGCAGCCGCTATACGATCGACCAGATCGACCCCGAGGTCTTTGCCGCTATCCAGAAGGAAAACCAGCGCCA
GGAAGACCACATCGAGCTGATCGCCTCCGAAAACTACACCTCGCCGGCCGTGATGGCTGCGCAAGGCTCGCAACTGACCA
ACAAGTACGCCGAGGGCTATCCCGGCAAGCGCTACTACGGTGGCTGCGAGTACGTCGACATCGTCGAACAGCTGGCCATC
GACCGCGTCAAGCAGCTGTTCGGCGCCGAAGCCGCCAACGTGCAGCCGAACTCGGGCTCGCAGGCCAACCAGGGCGTGTA
CTTCGCCGTGCTGAAGCCGGGCGACACCATCATGGGCATGAGCCTGGCCGAAGGCGGTCACCTGACCCACGGCATGGCGC
TGAACATGAGCGGCAAGTGGTTCAACGTGGTCAGCTACGGCCTGAACGCGCAGGAAGACATCGACTACGACGCGCTGGAA
AAGCTGGCACAAGAGAAGAAGCCGAAGCTGATCATCGCCGGCGCATCGGCCTTCGCGCTGCGCATCGACTTCGAGCGCAT
CGGCAAGGTCGCCAAGTCGATCGGTGCCTACTTCATGGTCGACATGGCCCACTACGCCGGCCTGATCGCCGCGGGCGTGT
ACCCGAACCCGGTGCCGCACGCCGACTTCGTCACCACCACCACGCACAAGAGCCTGCGCGGCCCGCGCGGCGGCGTGATC
CTGATGAAGGCCGAGCATGAGAAGGCCATCAACTCGTCGATCTTCCCGGGCATCCAGGGCGGTCCGCTGATGCACGTGAT
CGCTGGCAAGGCCGTCGCGTTCAAGGAAGCGCTGACGCCGGAATTCAAGGCTTACCAGGAACAAGTGGTCAAGAACGCCG
CCGTGCTGGCCGAAACGCTGATCGCGCGCGGCCTGCGCATTGTCTCGGGCCGCACCGAAAGCCACGTGATGCTGGTCGAC
CTGCGCGCCAAGAACATCACCGGCAAGGAAGCCGAGCGCATCCTGGGCGAAGCGCACCTGACCGTGAACAAGAACGCGAT
CCCGAACGATCCGGAAAAGCCGTTCGTGACCTCGGGCATTCGCGTCGGCTCGCCGGCCATGACCACGCGCGGCTTCAAGG
AAGAAGAAGCGCGTATCGTCGGCAACCTGATCGCCGACGTGCTGGACAACCCGCACGACGCGGGCAACATCGCCAGCGTG
CGCGAGCAGGTTTCCGCCCTGACCAAGCGTTTTCCGGTCTACGGCTGA

Upstream 100 bases:

>100_bases
GACGGTTCATCGACCCGCCGGCGTCGGCCGCCAATCGCTTTCCCCGCGCGCCCTACCCCGATGCAGTGATCGGATTCCCT
TTTTTCAGACGAGTTTCGCC

Downstream 100 bases:

>100_bases
TGCTGTGCATGGCCGCCCGCGAGGGCGGCCGGCAGCCCCGCGGGCGCGCCACCGCGCCGCTTGCGGGCCTCGCCAACCTG
CCCTATGCCATAGAAGGACA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT 1; Serine methylase 1

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MFERSRYTIDQIDPEVFAAIQKENQRQEDHIELIASENYTSPAVMAAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAI
DRVKQLFGAEAANVQPNSGSQANQGVYFAVLKPGDTIMGMSLAEGGHLTHGMALNMSGKWFNVVSYGLNAQEDIDYDALE
KLAQEKKPKLIIAGASAFALRIDFERIGKVAKSIGAYFMVDMAHYAGLIAAGVYPNPVPHADFVTTTTHKSLRGPRGGVI
LMKAEHEKAINSSIFPGIQGGPLMHVIAGKAVAFKEALTPEFKAYQEQVVKNAAVLAETLIARGLRIVSGRTESHVMLVD
LRAKNITGKEAERILGEAHLTVNKNAIPNDPEKPFVTSGIRVGSPAMTTRGFKEEEARIVGNLIADVLDNPHDAGNIASV
REQVSALTKRFPVYG

Sequences:

>Translated_415_residues
MFERSRYTIDQIDPEVFAAIQKENQRQEDHIELIASENYTSPAVMAAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAI
DRVKQLFGAEAANVQPNSGSQANQGVYFAVLKPGDTIMGMSLAEGGHLTHGMALNMSGKWFNVVSYGLNAQEDIDYDALE
KLAQEKKPKLIIAGASAFALRIDFERIGKVAKSIGAYFMVDMAHYAGLIAAGVYPNPVPHADFVTTTTHKSLRGPRGGVI
LMKAEHEKAINSSIFPGIQGGPLMHVIAGKAVAFKEALTPEFKAYQEQVVKNAAVLAETLIARGLRIVSGRTESHVMLVD
LRAKNITGKEAERILGEAHLTVNKNAIPNDPEKPFVTSGIRVGSPAMTTRGFKEEEARIVGNLIADVLDNPHDAGNIASV
REQVSALTKRFPVYG
>Mature_415_residues
MFERSRYTIDQIDPEVFAAIQKENQRQEDHIELIASENYTSPAVMAAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAI
DRVKQLFGAEAANVQPNSGSQANQGVYFAVLKPGDTIMGMSLAEGGHLTHGMALNMSGKWFNVVSYGLNAQEDIDYDALE
KLAQEKKPKLIIAGASAFALRIDFERIGKVAKSIGAYFMVDMAHYAGLIAAGVYPNPVPHADFVTTTTHKSLRGPRGGVI
LMKAEHEKAINSSIFPGIQGGPLMHVIAGKAVAFKEALTPEFKAYQEQVVKNAAVLAETLIARGLRIVSGRTESHVMLVD
LRAKNITGKEAERILGEAHLTVNKNAIPNDPEKPFVTSGIRVGSPAMTTRGFKEEEARIVGNLIADVLDNPHDAGNIASV
REQVSALTKRFPVYG

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=454, Percent_Identity=41.6299559471366, Blast_Score=344, Evalue=8e-95,
Organism=Homo sapiens, GI261862352, Length=454, Percent_Identity=41.6299559471366, Blast_Score=344, Evalue=1e-94,
Organism=Homo sapiens, GI261862350, Length=454, Percent_Identity=41.6299559471366, Blast_Score=344, Evalue=1e-94,
Organism=Homo sapiens, GI261862348, Length=454, Percent_Identity=41.6299559471366, Blast_Score=344, Evalue=1e-94,
Organism=Homo sapiens, GI261862346, Length=453, Percent_Identity=41.0596026490066, Blast_Score=335, Evalue=4e-92,
Organism=Homo sapiens, GI22547186, Length=397, Percent_Identity=45.3400503778337, Blast_Score=318, Evalue=6e-87,
Organism=Homo sapiens, GI22547189, Length=382, Percent_Identity=43.455497382199, Blast_Score=286, Evalue=3e-77,
Organism=Escherichia coli, GI1788902, Length=414, Percent_Identity=62.8019323671498, Blast_Score=523, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI25144732, Length=415, Percent_Identity=45.0602409638554, Blast_Score=354, Evalue=6e-98,
Organism=Caenorhabditis elegans, GI25144729, Length=415, Percent_Identity=45.0602409638554, Blast_Score=353, Evalue=7e-98,
Organism=Saccharomyces cerevisiae, GI6319739, Length=401, Percent_Identity=45.3865336658354, Blast_Score=348, Evalue=7e-97,
Organism=Saccharomyces cerevisiae, GI6323087, Length=394, Percent_Identity=47.4619289340102, Blast_Score=338, Evalue=8e-94,
Organism=Drosophila melanogaster, GI24640005, Length=452, Percent_Identity=42.6991150442478, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI221329721, Length=452, Percent_Identity=42.6991150442478, Blast_Score=358, Evalue=3e-99,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA1_CUPPJ (Q474L3)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_295014.1
- ProteinModelPortal:   Q474L3
- SMR:   Q474L3
- GeneID:   3609970
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A0790
- NMPDR:   fig|264198.3.peg.1304
- HOGENOM:   HBG301263
- OMA:   WTAIQDE
- ProtClustDB:   PRK00011
- BioCyc:   REUT264198:REUT_A0790-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45088; Mature: 45088

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFERSRYTIDQIDPEVFAAIQKENQRQEDHIELIASENYTSPAVMAAQGSQLTNKYAEGY
CCCCCCCCHHHCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCHHHHHHHCCC
PGKRYYGGCEYVDIVEQLAIDRVKQLFGAEAANVQPNSGSQANQGVYFAVLKPGDTIMGM
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEE
SLAEGGHLTHGMALNMSGKWFNVVSYGLNAQEDIDYDALEKLAQEKKPKLIIAGASAFAL
EECCCCCEECCEEEECCCCEEEHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCEEEE
RIDFERIGKVAKSIGAYFMVDMAHYAGLIAAGVYPNPVPHADFVTTTTHKSLRGPRGGVI
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHCCCCCCEE
LMKAEHEKAINSSIFPGIQGGPLMHVIAGKAVAFKEALTPEFKAYQEQVVKNAAVLAETL
EEECCHHHHHHHCCCCCCCCCCEEEEHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
IARGLRIVSGRTESHVMLVDLRAKNITGKEAERILGEAHLTVNKNAIPNDPEKPFVTSGI
HHCCEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHEEEECCCCCCCCCCCCEEECCE
RVGSPAMTTRGFKEEEARIVGNLIADVLDNPHDAGNIASVREQVSALTKRFPVYG
EECCCCHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFERSRYTIDQIDPEVFAAIQKENQRQEDHIELIASENYTSPAVMAAQGSQLTNKYAEGY
CCCCCCCCHHHCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCHHHHHHHCCC
PGKRYYGGCEYVDIVEQLAIDRVKQLFGAEAANVQPNSGSQANQGVYFAVLKPGDTIMGM
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEE
SLAEGGHLTHGMALNMSGKWFNVVSYGLNAQEDIDYDALEKLAQEKKPKLIIAGASAFAL
EECCCCCEECCEEEECCCCEEEHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCEEEE
RIDFERIGKVAKSIGAYFMVDMAHYAGLIAAGVYPNPVPHADFVTTTTHKSLRGPRGGVI
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHCCCCCCEE
LMKAEHEKAINSSIFPGIQGGPLMHVIAGKAVAFKEALTPEFKAYQEQVVKNAAVLAETL
EEECCHHHHHHHCCCCCCCCCCEEEEHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
IARGLRIVSGRTESHVMLVDLRAKNITGKEAERILGEAHLTVNKNAIPNDPEKPFVTSGI
HHCCEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHEEEECCCCCCCCCCCCEEECCE
RVGSPAMTTRGFKEEEARIVGNLIADVLDNPHDAGNIASVREQVSALTKRFPVYG
EECCCCHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA