Click here to switch to the map view.

The map label for this gene is thrS

Identifier: 71908290

GI number: 71908290

Start: 2872722

End: 2874638

Strand: Reverse

Name: thrS

Synonym: Daro_2677

Alternate gene names: 71908290

Gene position: 2874638-2872722 (Counterclockwise)

Preceding gene: 71908293

Following gene: 71908289

Centisome position: 63.87

GC content: 57.69

Gene sequence:

>1917_bases
ATGCCCGATATCAAACTGCCTGATGGTTCCATCCGCTCCTATGAGCAAGCGGTCACCGTTGCCGAAGTCGCCGCCAGTAT
TGGTGCTGGCTTGGCCCGTGCTGCGCTGGCTGGCAAGATTGGTGGCCAGTTGGTCGATACTTCCTATTTGATTGAGCAAA
ACGCCGATCTGGCCATCATCACTGAGCGCGATGCCGAGGGTTTGGAATTGATCCGCCATTCGACTGCGCACCTGTTGGCC
TATGCCGTCAAGGAGCTCTTCCCGGAGGCGCAGGTTACGATCGGCCCGGTCATCGAGAATGGCTTCTACTACGACTTTGC
CTACAAGCGTCCGTTTACCCCGGAAGATCTGGTCGCCATCGAGAAGCGCATGGCCGAACTGGCCAAAAAGGACATTCCGG
TCAGCCGCGAAGTCTGGGCGCGCGACGATGCGGTCAAGTTCTTCCTTGATCTCGGCGAGAAATACAAAGCTGAACTGATC
GGCGCTATTCCGGCTGATCAGCAGGTATCGCTGTATCGTGAAGGTGATTTCATTGACCTCTGCCGCGGTCCGCACGTGCC
GACTACGGCCAAACTCAAGGTCTTCAAGTTGATGAAGGTGGCTGGTGCCTACTGGCGCGGCGATCATCGTAACGAGCAGT
TGCAGCGCATCTACGGTACGGCCTGGGCCAAGAAGGAAGATCTCGACGCCTACCTGCACATGCTAGAAGAAGCCGAGAAG
CGCGATCACCGGCGTCTGGGTAAGCAATATGACCTGTTTCACATGCAGGACGAAGCGCCGGGTCTGGTTTTCTGGCACCC
GAAAGGCTGGGCAATCTGGCAGGAAATTGAAGGCTACATGCGCGCGGTTTACCGCAACAACGGCTACCAGGAAGTGCGTT
GCCCGCAGATTCTCGACAAGTCGCTGTGGGAAAAGTCTGGCCACTGGGAGCACTACAAGGACAACATGTTCACGACCTCG
TCGGAAAATCGCGACTACGCGGTCAAGCCGATGAACTGCCCGGGCCACGTGCAGGTCTTCAATGCCGGCCTGCGCTCTTA
TCGCGAACTGCCGCTGCGTTACGGCGAGTTCGGCTCCTGCCATCGCAACGAACCGGCTGGCGCCTTGCATGGCCTGATGC
GTGTCCGAGGATTCGTGCAGGATGATGGACATATCTTCTGTACCGAAGATCAGATCGAGTCCGAAGTCACCGCCTTCAAT
GCGCTGGTCAAGAAGGTTTATGCTGATTTTGGGTTCAACGATGTTGCGGTCAAGCTCGCGCTGCGTCCGGATAGCCGGGT
TGGTGCCGATGATGTCTGGGATAGGGCAGAGGATGCGCTGCGTCAGGGGTTGCGCGCTTCCGGGCTGGAGTGGACGGAAC
TGCCGGGCGAGGGCGCCTTCTACGGCCCGAAGATCGAATTCCATATCAGGGATGCCATCGGCCGTTCCTGGCAGTGTGGC
ACCATGCAGGTCGACTTCTCTATGCCGGGGCGTTTGGGTGCCGAGTATGTCGGTGCGGACGATACGCGCAAGGTGCCGGT
CATGCTGCATCGGGCCATTCTTGGTTCGCTGGAGCGTTTCATCGGCATCCTGATCGAAAACTTCGCCGGTGCCTTGCCGT
TGTGGTTGGCGCCTGTGCAGGTGGTGGTGCTGAATATCTCCGAAAAGCAGGCCGATTACGCGGCTGAAGTCGCACAAAAG
CTACACTCGGCGGGCTTTCGGGCTGAGGCGGATTTGCGTAACGAGAAAATTACCTATAAAATCCGGGAACATAGCTTGAA
CCGGCTGCCTTATCAGCTCGTTGTGGGCGATAAGGAAAAAGCGGACGGACTGGTAGCCGTGCGTACTCGCGGTGGTCAGG
ACCTCGGACAGATGCCCGTAGATGTCTTGATCAAGCGACTTCAAGAAGAAGTCGTGGCGCGTAGTGGCACGGCTTAA

Upstream 100 bases:

>100_bases
CGCAGTTCAGAAACTCTAGTCGAGTCAGCTCTCGTTTATTAAAAAAGTGCGGCTCACCCCGCACTTTTTTTTCGTCCAGA
TTTTTTCCAGAGAATCCGCC

Downstream 100 bases:

>100_bases
TTATTGTTGTTTTCGGGGGTTTTACCATAGCTCAGAACAAGGCGCATCGCCTCAACGAAGAAATCACGGTTCCTGAGATT
CGTCTCCAGGGTGCAGAAGG

Product: threonyl-tRNA synthetase / Ser-tRNA(Thr) hydrolase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 638; Mature: 637

Protein sequence:

>638_residues
MPDIKLPDGSIRSYEQAVTVAEVAASIGAGLARAALAGKIGGQLVDTSYLIEQNADLAIITERDAEGLELIRHSTAHLLA
YAVKELFPEAQVTIGPVIENGFYYDFAYKRPFTPEDLVAIEKRMAELAKKDIPVSREVWARDDAVKFFLDLGEKYKAELI
GAIPADQQVSLYREGDFIDLCRGPHVPTTAKLKVFKLMKVAGAYWRGDHRNEQLQRIYGTAWAKKEDLDAYLHMLEEAEK
RDHRRLGKQYDLFHMQDEAPGLVFWHPKGWAIWQEIEGYMRAVYRNNGYQEVRCPQILDKSLWEKSGHWEHYKDNMFTTS
SENRDYAVKPMNCPGHVQVFNAGLRSYRELPLRYGEFGSCHRNEPAGALHGLMRVRGFVQDDGHIFCTEDQIESEVTAFN
ALVKKVYADFGFNDVAVKLALRPDSRVGADDVWDRAEDALRQGLRASGLEWTELPGEGAFYGPKIEFHIRDAIGRSWQCG
TMQVDFSMPGRLGAEYVGADDTRKVPVMLHRAILGSLERFIGILIENFAGALPLWLAPVQVVVLNISEKQADYAAEVAQK
LHSAGFRAEADLRNEKITYKIREHSLNRLPYQLVVGDKEKADGLVAVRTRGGQDLGQMPVDVLIKRLQEEVVARSGTA

Sequences:

>Translated_638_residues
MPDIKLPDGSIRSYEQAVTVAEVAASIGAGLARAALAGKIGGQLVDTSYLIEQNADLAIITERDAEGLELIRHSTAHLLA
YAVKELFPEAQVTIGPVIENGFYYDFAYKRPFTPEDLVAIEKRMAELAKKDIPVSREVWARDDAVKFFLDLGEKYKAELI
GAIPADQQVSLYREGDFIDLCRGPHVPTTAKLKVFKLMKVAGAYWRGDHRNEQLQRIYGTAWAKKEDLDAYLHMLEEAEK
RDHRRLGKQYDLFHMQDEAPGLVFWHPKGWAIWQEIEGYMRAVYRNNGYQEVRCPQILDKSLWEKSGHWEHYKDNMFTTS
SENRDYAVKPMNCPGHVQVFNAGLRSYRELPLRYGEFGSCHRNEPAGALHGLMRVRGFVQDDGHIFCTEDQIESEVTAFN
ALVKKVYADFGFNDVAVKLALRPDSRVGADDVWDRAEDALRQGLRASGLEWTELPGEGAFYGPKIEFHIRDAIGRSWQCG
TMQVDFSMPGRLGAEYVGADDTRKVPVMLHRAILGSLERFIGILIENFAGALPLWLAPVQVVVLNISEKQADYAAEVAQK
LHSAGFRAEADLRNEKITYKIREHSLNRLPYQLVVGDKEKADGLVAVRTRGGQDLGQMPVDVLIKRLQEEVVARSGTA
>Mature_637_residues
PDIKLPDGSIRSYEQAVTVAEVAASIGAGLARAALAGKIGGQLVDTSYLIEQNADLAIITERDAEGLELIRHSTAHLLAY
AVKELFPEAQVTIGPVIENGFYYDFAYKRPFTPEDLVAIEKRMAELAKKDIPVSREVWARDDAVKFFLDLGEKYKAELIG
AIPADQQVSLYREGDFIDLCRGPHVPTTAKLKVFKLMKVAGAYWRGDHRNEQLQRIYGTAWAKKEDLDAYLHMLEEAEKR
DHRRLGKQYDLFHMQDEAPGLVFWHPKGWAIWQEIEGYMRAVYRNNGYQEVRCPQILDKSLWEKSGHWEHYKDNMFTTSS
ENRDYAVKPMNCPGHVQVFNAGLRSYRELPLRYGEFGSCHRNEPAGALHGLMRVRGFVQDDGHIFCTEDQIESEVTAFNA
LVKKVYADFGFNDVAVKLALRPDSRVGADDVWDRAEDALRQGLRASGLEWTELPGEGAFYGPKIEFHIRDAIGRSWQCGT
MQVDFSMPGRLGAEYVGADDTRKVPVMLHRAILGSLERFIGILIENFAGALPLWLAPVQVVVLNISEKQADYAAEVAQKL
HSAGFRAEADLRNEKITYKIREHSLNRLPYQLVVGDKEKADGLVAVRTRGGQDLGQMPVDVLIKRLQEEVVARSGTA

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38570111, Length=639, Percent_Identity=39.2801251956182, Blast_Score=476, Evalue=1e-134,
Organism=Homo sapiens, GI38202255, Length=639, Percent_Identity=39.2801251956182, Blast_Score=467, Evalue=1e-131,
Organism=Homo sapiens, GI20070344, Length=647, Percent_Identity=37.7125193199382, Blast_Score=426, Evalue=1e-119,
Organism=Escherichia coli, GI1788013, Length=636, Percent_Identity=58.6477987421384, Blast_Score=799, Evalue=0.0,
Organism=Escherichia coli, GI1786392, Length=163, Percent_Identity=28.2208588957055, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71984184, Length=637, Percent_Identity=38.9324960753532, Blast_Score=475, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI71984192, Length=637, Percent_Identity=38.9324960753532, Blast_Score=475, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6322112, Length=629, Percent_Identity=39.7456279809221, Blast_Score=467, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6322654, Length=398, Percent_Identity=37.9396984924623, Blast_Score=293, Evalue=7e-80,
Organism=Drosophila melanogaster, GI24583842, Length=629, Percent_Identity=40.6995230524642, Blast_Score=497, Evalue=1e-141,
Organism=Drosophila melanogaster, GI28574141, Length=629, Percent_Identity=40.6995230524642, Blast_Score=497, Evalue=1e-141,
Organism=Drosophila melanogaster, GI24583839, Length=629, Percent_Identity=40.6995230524642, Blast_Score=497, Evalue=1e-140,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_DECAR (Q47CM4)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_285877.1
- ProteinModelPortal:   Q47CM4
- SMR:   Q47CM4
- STRING:   Q47CM4
- GeneID:   3567018
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_2677
- NMPDR:   fig|159087.4.peg.3453
- eggNOG:   COG0441
- HOGENOM:   HBG352811
- OMA:   TAKDPEC
- ProtClustDB:   CLSK2768589
- BioCyc:   DARO159087:DARO_2677-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 71869; Mature: 71737

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDIKLPDGSIRSYEQAVTVAEVAASIGAGLARAALAGKIGGQLVDTSYLIEQNADLAII
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHEECCCCEEEE
TERDAEGLELIRHSTAHLLAYAVKELFPEAQVTIGPVIENGFYYDFAYKRPFTPEDLVAI
ECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEECCCCCHHHHHHH
EKRMAELAKKDIPVSREVWARDDAVKFFLDLGEKYKAELIGAIPADQQVSLYREGDFIDL
HHHHHHHHHHCCCCCHHHHCCHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEECCCEEEE
CRGPHVPTTAKLKVFKLMKVAGAYWRGDHRNEQLQRIYGTAWAKKEDLDAYLHMLEEAEK
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RDHRRLGKQYDLFHMQDEAPGLVFWHPKGWAIWQEIEGYMRAVYRNNGYQEVRCPQILDK
HHHHHCCCCCCEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
SLWEKSGHWEHYKDNMFTTSSENRDYAVKPMNCPGHVQVFNAGLRSYRELPLRYGEFGSC
HHHHCCCCCCCCCCCEEECCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
HRNEPAGALHGLMRVRGFVQDDGHIFCTEDQIESEVTAFNALVKKVYADFGFNDVAVKLA
CCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
LRPDSRVGADDVWDRAEDALRQGLRASGLEWTELPGEGAFYGPKIEFHIRDAIGRSWQCG
ECCCCCCCCHHHHHHHHHHHHHCHHHCCCCEECCCCCCCEECCEEEEEEHHHCCCCCCCC
TMQVDFSMPGRLGAEYVGADDTRKVPVMLHRAILGSLERFIGILIENFAGALPLWLAPVQ
EEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCEE
VVVLNISEKQADYAAEVAQKLHSAGFRAEADLRNEKITYKIREHSLNRLPYQLVVGDKEK
EEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHCCCCCCCEEEEECCCCC
ADGLVAVRTRGGQDLGQMPVDVLIKRLQEEVVARSGTA
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PDIKLPDGSIRSYEQAVTVAEVAASIGAGLARAALAGKIGGQLVDTSYLIEQNADLAII
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHEECCCCEEEE
TERDAEGLELIRHSTAHLLAYAVKELFPEAQVTIGPVIENGFYYDFAYKRPFTPEDLVAI
ECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEECCCCCHHHHHHH
EKRMAELAKKDIPVSREVWARDDAVKFFLDLGEKYKAELIGAIPADQQVSLYREGDFIDL
HHHHHHHHHHCCCCCHHHHCCHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEECCCEEEE
CRGPHVPTTAKLKVFKLMKVAGAYWRGDHRNEQLQRIYGTAWAKKEDLDAYLHMLEEAEK
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RDHRRLGKQYDLFHMQDEAPGLVFWHPKGWAIWQEIEGYMRAVYRNNGYQEVRCPQILDK
HHHHHCCCCCCEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
SLWEKSGHWEHYKDNMFTTSSENRDYAVKPMNCPGHVQVFNAGLRSYRELPLRYGEFGSC
HHHHCCCCCCCCCCCEEECCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
HRNEPAGALHGLMRVRGFVQDDGHIFCTEDQIESEVTAFNALVKKVYADFGFNDVAVKLA
CCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
LRPDSRVGADDVWDRAEDALRQGLRASGLEWTELPGEGAFYGPKIEFHIRDAIGRSWQCG
ECCCCCCCCHHHHHHHHHHHHHCHHHCCCCEECCCCCCCEECCEEEEEEHHHCCCCCCCC
TMQVDFSMPGRLGAEYVGADDTRKVPVMLHRAILGSLERFIGILIENFAGALPLWLAPVQ
EEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCEE
VVVLNISEKQADYAAEVAQKLHSAGFRAEADLRNEKITYKIREHSLNRLPYQLVVGDKEK
EEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHCCCCCCCEEEEECCCCC
ADGLVAVRTRGGQDLGQMPVDVLIKRLQEEVVARSGTA
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA