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The map label for this gene is rpoS [H]

Identifier: 71908137

GI number: 71908137

Start: 2719020

End: 2719949

Strand: Reverse

Name: rpoS [H]

Synonym: Daro_2521

Alternate gene names: 71908137

Gene position: 2719949-2719020 (Counterclockwise)

Preceding gene: 71908138

Following gene: 71908132

Centisome position: 60.43

GC content: 57.85

Gene sequence:

>930_bases
ATGAGCGATACCGGCGAGGGTCTGGAGGAAGAGTTCGAGGGGCAGCCCGATGAAACGGTCCTGCCTCCTGAGCCTGAACG
CGTTCTGCCGGAAATTGAACTGCTTGAGGATGTCACCCAGCTTTACCTGAGTGAGATTGGCGCCAAGCCGCTCCTGACTC
CCGCTGAAGAACTGGCCACCTCCCGGCTTGTCCGGGAAGGTGACTTTAGCGCACGCCAGCGCATGATCGAACACAATCTG
CGGCTGGTTGTAAATATCGCCAAACATTACCTGAATCGGGGGATTCCTCTGCTCGACCTGATCGAGGAGGGAAATCTGGG
ATTGATCCATGCCTTGGAGAAATTCGATCCGGAGCGTGGCTTCAGGTTCTCGACCTATGCGACTTGGTGGATACGCCAGA
ATATCGAGCGCGGCATCATGAACCAGTCGCGGACGATCCGTTTGCCGGTGCATATCGTCAAGGAAATCAATGTCGTCCTG
CGGGCTATGCGCCACTTGGAATCGGCGGACAAGCGGGATTCGACGGTCGAGCGCATTGCGCTCCTGATTGATCGGCCGGT
CGCCGATGTACGGCGTATCCTGTCGCTCAATGAGCATATTGCGTCTCTCGATGCGCCACTGGAAATCGACCCCAATCACA
CGATGGCCGAGGTTATCGCCGACGATGGAGAGAGCGATCCGGAGTCCTTGCTGCAGTCGAGCGAGGTCGGTTCGCTGGTT
GATGACTGGCTGGAGCAATTGACCGAACGGCAACGTTCAGTGATCGAGCGCCGCTATGGCCTGAGCGGGGCCGATTTGGC
GACGCTGGACGTGATAGCCTGCGATCTCGGATTGACCCGCGAACGGGTCCGGCAGATCCAGATGGAAGGTCTGGATCGCC
TGCGCAAGATCATCAAGCGCGGCAATATTTCTCGCGATTCGTTGCTGTAG

Upstream 100 bases:

>100_bases
TTGCCGAAATGGGCAACACGGATGCGGAGTCGGTCAAGCTGCATTTCGAAATCCGCAAACAGGGTAAGCCGGTTGATCCG
GCACAGTATCTACCGAAGCG

Downstream 100 bases:

>100_bases
CCGGTTGGCTGGCGCACATCCGGCTGCGGATTTCTTCTGCCAGCTGGAAAACCGACATGGCGTAGAAGCTGGAGCGGTTG
TAGCGGGTGATCACGTAAAA

Product: RNA polymerase sigma factor RpoS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MSDTGEGLEEEFEGQPDETVLPPEPERVLPEIELLEDVTQLYLSEIGAKPLLTPAEELATSRLVREGDFSARQRMIEHNL
RLVVNIAKHYLNRGIPLLDLIEEGNLGLIHALEKFDPERGFRFSTYATWWIRQNIERGIMNQSRTIRLPVHIVKEINVVL
RAMRHLESADKRDSTVERIALLIDRPVADVRRILSLNEHIASLDAPLEIDPNHTMAEVIADDGESDPESLLQSSEVGSLV
DDWLEQLTERQRSVIERRYGLSGADLATLDVIACDLGLTRERVRQIQMEGLDRLRKIIKRGNISRDSLL

Sequences:

>Translated_309_residues
MSDTGEGLEEEFEGQPDETVLPPEPERVLPEIELLEDVTQLYLSEIGAKPLLTPAEELATSRLVREGDFSARQRMIEHNL
RLVVNIAKHYLNRGIPLLDLIEEGNLGLIHALEKFDPERGFRFSTYATWWIRQNIERGIMNQSRTIRLPVHIVKEINVVL
RAMRHLESADKRDSTVERIALLIDRPVADVRRILSLNEHIASLDAPLEIDPNHTMAEVIADDGESDPESLLQSSEVGSLV
DDWLEQLTERQRSVIERRYGLSGADLATLDVIACDLGLTRERVRQIQMEGLDRLRKIIKRGNISRDSLL
>Mature_308_residues
SDTGEGLEEEFEGQPDETVLPPEPERVLPEIELLEDVTQLYLSEIGAKPLLTPAEELATSRLVREGDFSARQRMIEHNLR
LVVNIAKHYLNRGIPLLDLIEEGNLGLIHALEKFDPERGFRFSTYATWWIRQNIERGIMNQSRTIRLPVHIVKEINVVLR
AMRHLESADKRDSTVERIALLIDRPVADVRRILSLNEHIASLDAPLEIDPNHTMAEVIADDGESDPESLLQSSEVGSLVD
DWLEQLTERQRSVIERRYGLSGADLATLDVIACDLGLTRERVRQIQMEGLDRLRKIIKRGNISRDSLL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in acid resistance [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=273, Percent_Identity=55.6776556776557, Blast_Score=301, Evalue=3e-83,
Organism=Escherichia coli, GI1789448, Length=225, Percent_Identity=39.1111111111111, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1789871, Length=277, Percent_Identity=27.4368231046931, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 35185; Mature: 35053

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTGEGLEEEFEGQPDETVLPPEPERVLPEIELLEDVTQLYLSEIGAKPLLTPAEELAT
CCCCCCCHHHHHCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
SRLVREGDFSARQRMIEHNLRLVVNIAKHYLNRGIPLLDLIEEGNLGLIHALEKFDPERG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHCCCCCC
FRFSTYATWWIRQNIERGIMNQSRTIRLPVHIVKEINVVLRAMRHLESADKRDSTVERIA
CEEHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LLIDRPVADVRRILSLNEHIASLDAPLEIDPNHTMAEVIADDGESDPESLLQSSEVGSLV
HHHCCCHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
DDWLEQLTERQRSVIERRYGLSGADLATLDVIACDLGLTRERVRQIQMEGLDRLRKIIKR
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
GNISRDSLL
CCCCHHCCC
>Mature Secondary Structure 
SDTGEGLEEEFEGQPDETVLPPEPERVLPEIELLEDVTQLYLSEIGAKPLLTPAEELAT
CCCCCCHHHHHCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
SRLVREGDFSARQRMIEHNLRLVVNIAKHYLNRGIPLLDLIEEGNLGLIHALEKFDPERG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHCCCCCC
FRFSTYATWWIRQNIERGIMNQSRTIRLPVHIVKEINVVLRAMRHLESADKRDSTVERIA
CEEHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LLIDRPVADVRRILSLNEHIASLDAPLEIDPNHTMAEVIADDGESDPESLLQSSEVGSLV
HHHCCCHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
DDWLEQLTERQRSVIERRYGLSGADLATLDVIACDLGLTRERVRQIQMEGLDRLRKIIKR
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
GNISRDSLL
CCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7959068; 10984043 [H]