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The map label for this gene is rluB [H]

Identifier: 71908073

GI number: 71908073

Start: 2656524

End: 2657546

Strand: Reverse

Name: rluB [H]

Synonym: Daro_2455

Alternate gene names: 71908073

Gene position: 2657546-2656524 (Counterclockwise)

Preceding gene: 71908074

Following gene: 71908072

Centisome position: 59.04

GC content: 59.92

Gene sequence:

>1023_bases
ATGAAAAATAAACGCACACCATTCCGTCAATCGTCGAACAAATCCGAGGGGGCTGCCGAGCGCCGACCGGAAGATGCAGG
TCCACGTGTCGGCGCACCGTCGCGTGGACGCGCGGCCGCGCAGCGTGACCAGGTGCCAGGCGATGCTCAGGGGGCGAAAC
CTGCGCCGCGCAGAAAGCCGGCACCCAATACCGGCGGAAGAGCCAATCGTGGTAGCGTCGCCCGCGACGGACGGCCGCTT
GCTGAGGCCAAGCCAGTGCGTTTGCAGAAGGTGCTGGCCGAGGCTGGTGTCGGTTCGCGTCGCGAAATGGAAGAGTGGAT
TGCTGCAGGCAAGGTCAGCGTCAATGGCGTCGTGGCGACTGTAGGGCAATCGGTTGTGAATTCCGACAAGGTCAAAATTG
GTGGCCGCCTGATCAATATCCGCTTTACGGGCAGTTCTCGTCCGCCGCGCGTCTTGATGTATCACAAGCCGGAAGGCGAA
ATCGTTTCGCGCGACGACCCGGATGGTCGGCCGTCGGTGTTTGCTGCGCTGCCACGGATGCGCGGTGGGCGGTGGATCAA
TGTCGGTCGCCTCGACTTCAATACCTCGGGTTTGCTGTTATTCACTACTTCTGGTGAGCTGGCCAACAAACTGATGCATC
CGAGTTCGGAACTGGTTCGCGAGTACGCCGTGCGTGTTCTTGGTGAACTGACCCTGGATGCACAACAGAAGCTGTTGCAC
GGCGTCGAACTGGAAGATGGTCGAGCGAACTTTGGTTCGCTACACGACGGTGGTGGCGAGGGGGCGAACCACTGGTACCG
AGTAACGATCTCCGAGGGGCGTAACCGTGAGGTCCGTCGCATGTTCGAGGCGGTCGGTTGCACGGTCAGCCGATTGATTC
GCGTTCGCTATGGCCCGTTCATCCTGCCGCCGCAACTGAAACGAGGTATGGCCCGCGAGTTGAAAGAGGCGGAAATCAAA
ATGCTGATGCGAGAACTCGAAAACATGCCATCGTCTCAGCGAAAAGGCCCTGAAGGCACGTAA

Upstream 100 bases:

>100_bases
TATTTGCCACGACCAAGCAATTTCTCGATGATCTCGGTCTCAGAAGCGTCAGCGAACTGCCGCCGTTAGAACAAATCAGC
CAGACACTGGAGCTGGAACA

Downstream 100 bases:

>100_bases
ATCGATTTACTGGGGGGAATAAACTCGTTATAATTCACGGGTTTGTTCCTCCCGTCGTTTCTGGCGGGCATCTTTTTATT
GTGATGTGGGCGTTTCGCCC

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKNKRTPFRQSSNKSEGAAERRPEDAGPRVGAPSRGRAAAQRDQVPGDAQGAKPAPRRKPAPNTGGRANRGSVARDGRPL
AEAKPVRLQKVLAEAGVGSRREMEEWIAAGKVSVNGVVATVGQSVVNSDKVKIGGRLINIRFTGSSRPPRVLMYHKPEGE
IVSRDDPDGRPSVFAALPRMRGGRWINVGRLDFNTSGLLLFTTSGELANKLMHPSSELVREYAVRVLGELTLDAQQKLLH
GVELEDGRANFGSLHDGGGEGANHWYRVTISEGRNREVRRMFEAVGCTVSRLIRVRYGPFILPPQLKRGMARELKEAEIK
MLMRELENMPSSQRKGPEGT

Sequences:

>Translated_340_residues
MKNKRTPFRQSSNKSEGAAERRPEDAGPRVGAPSRGRAAAQRDQVPGDAQGAKPAPRRKPAPNTGGRANRGSVARDGRPL
AEAKPVRLQKVLAEAGVGSRREMEEWIAAGKVSVNGVVATVGQSVVNSDKVKIGGRLINIRFTGSSRPPRVLMYHKPEGE
IVSRDDPDGRPSVFAALPRMRGGRWINVGRLDFNTSGLLLFTTSGELANKLMHPSSELVREYAVRVLGELTLDAQQKLLH
GVELEDGRANFGSLHDGGGEGANHWYRVTISEGRNREVRRMFEAVGCTVSRLIRVRYGPFILPPQLKRGMARELKEAEIK
MLMRELENMPSSQRKGPEGT
>Mature_340_residues
MKNKRTPFRQSSNKSEGAAERRPEDAGPRVGAPSRGRAAAQRDQVPGDAQGAKPAPRRKPAPNTGGRANRGSVARDGRPL
AEAKPVRLQKVLAEAGVGSRREMEEWIAAGKVSVNGVVATVGQSVVNSDKVKIGGRLINIRFTGSSRPPRVLMYHKPEGE
IVSRDDPDGRPSVFAALPRMRGGRWINVGRLDFNTSGLLLFTTSGELANKLMHPSSELVREYAVRVLGELTLDAQQKLLH
GVELEDGRANFGSLHDGGGEGANHWYRVTISEGRNREVRRMFEAVGCTVSRLIRVRYGPFILPPQLKRGMARELKEAEIK
MLMRELENMPSSQRKGPEGT

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=246, Percent_Identity=57.3170731707317, Blast_Score=262, Evalue=3e-71,
Organism=Escherichia coli, GI1790453, Length=246, Percent_Identity=30.8943089430894, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87081838, Length=191, Percent_Identity=31.413612565445, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 37233; Mature: 37233

Theoretical pI: Translated: 11.29; Mature: 11.29

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKRTPFRQSSNKSEGAAERRPEDAGPRVGAPSRGRAAAQRDQVPGDAQGAKPAPRRKP
CCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCC
APNTGGRANRGSVARDGRPLAEAKPVRLQKVLAEAGVGSRREMEEWIAAGKVSVNGVVAT
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHH
VGQSVVNSDKVKIGGRLINIRFTGSSRPPRVLMYHKPEGEIVSRDDPDGRPSVFAALPRM
HHHHHHCCCCEEECCEEEEEEECCCCCCCEEEEEECCCCCEECCCCCCCCHHHHHHHHHC
RGGRWINVGRLDFNTSGLLLFTTSGELANKLMHPSSELVREYAVRVLGELTLDAQQKLLH
CCCEEEEEEEEEECCCCEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GVELEDGRANFGSLHDGGGEGANHWYRVTISEGRNREVRRMFEAVGCTVSRLIRVRYGPF
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCE
ILPPQLKRGMARELKEAEIKMLMRELENMPSSQRKGPEGT
ECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCC
>Mature Secondary Structure
MKNKRTPFRQSSNKSEGAAERRPEDAGPRVGAPSRGRAAAQRDQVPGDAQGAKPAPRRKP
CCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCC
APNTGGRANRGSVARDGRPLAEAKPVRLQKVLAEAGVGSRREMEEWIAAGKVSVNGVVAT
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHH
VGQSVVNSDKVKIGGRLINIRFTGSSRPPRVLMYHKPEGEIVSRDDPDGRPSVFAALPRM
HHHHHHCCCCEEECCEEEEEEECCCCCCCEEEEEECCCCCEECCCCCCCCHHHHHHHHHC
RGGRWINVGRLDFNTSGLLLFTTSGELANKLMHPSSELVREYAVRVLGELTLDAQQKLLH
CCCEEEEEEEEEECCCCEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GVELEDGRANFGSLHDGGGEGANHWYRVTISEGRNREVRRMFEAVGCTVSRLIRVRYGPF
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCE
ILPPQLKRGMARELKEAEIKMLMRELENMPSSQRKGPEGT
ECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]