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The map label for this gene is def2 [H]

Identifier: 71907756

GI number: 71907756

Start: 2302151

End: 2302765

Strand: Reverse

Name: def2 [H]

Synonym: Daro_2132

Alternate gene names: 71907756

Gene position: 2302765-2302151 (Counterclockwise)

Preceding gene: 71907761

Following gene: 71907755

Centisome position: 51.16

GC content: 59.84

Gene sequence:

>615_bases
ATGTGCTTATGCAGCACCATGGGGTTTGTCGATAGCCGCCGGTTGTGGTGTCATTGGCCCGGACAATTTTGGAAAGTTAC
AGCGATGGCGGCAAGACCGATCCTGCGTATGGGAGAACCACTCCTGTTTGAAGTGGCAAAAGCGGTGACCGAGTTCGGGA
CCTCCGAACTGCGTGCGCTGATCGAGGACATGTTCGATTCGATGGAGGCTGCCGGTGGCGTCGGGCTCGCCGCGCCGCAG
ATCGGGATCGGTTTGCAGATGGTGATTTTCGGCTTCGAAAAGAGCGAGCGTTATCCCGACGCCGAAGCGGTGCCGCTGAC
TGTCCTGATCAACCCGCTCATCACGCCCGTGGGCGACGAGGAAGAACTTGGCTGGGAAGGCTGCCTGTCGGTACCTGGTC
TGCGGGGTGAAGTGCCGCGCCATACCCGCATCCGCTACCAGGGCTTCGACCCGGAAGGCACACCAATCGATCGTACCGTG
GAGGGCTTCCATGCCCGTGTTGTCCAGCATGAGTGTGATCACCTGATCGGTCGGCTTTACCCGTCGCGGATCCGTGACTT
CTCGCGCTTCGGCTTTACCGACATTCTCTTCCCGGAACTGGTCCGGGAAGATTGA

Upstream 100 bases:

>100_bases
AGCCCGGGAACGATCAGGATGGTGGTCATGGAGATGTCCTCGTGAAATCGGTTGATCCATTGTCGAAGCAGGGCCGGGGA
AGGGGAACGATGATGTTCTT

Downstream 100 bases:

>100_bases
GGGCCACTATTTCGATCGCCTCTCATCCTTCCAGCGGGCGATAGCTCCAGCGCGGAATGATGAGCAGGGCAAGCACCGAA
ATGCCGGCCCCCAGAATCAG

Product: formylmethionine deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MCLCSTMGFVDSRRLWCHWPGQFWKVTAMAARPILRMGEPLLFEVAKAVTEFGTSELRALIEDMFDSMEAAGGVGLAAPQ
IGIGLQMVIFGFEKSERYPDAEAVPLTVLINPLITPVGDEEELGWEGCLSVPGLRGEVPRHTRIRYQGFDPEGTPIDRTV
EGFHARVVQHECDHLIGRLYPSRIRDFSRFGFTDILFPELVRED

Sequences:

>Translated_204_residues
MCLCSTMGFVDSRRLWCHWPGQFWKVTAMAARPILRMGEPLLFEVAKAVTEFGTSELRALIEDMFDSMEAAGGVGLAAPQ
IGIGLQMVIFGFEKSERYPDAEAVPLTVLINPLITPVGDEEELGWEGCLSVPGLRGEVPRHTRIRYQGFDPEGTPIDRTV
EGFHARVVQHECDHLIGRLYPSRIRDFSRFGFTDILFPELVRED
>Mature_204_residues
MCLCSTMGFVDSRRLWCHWPGQFWKVTAMAARPILRMGEPLLFEVAKAVTEFGTSELRALIEDMFDSMEAAGGVGLAAPQ
IGIGLQMVIFGFEKSERYPDAEAVPLTVLINPLITPVGDEEELGWEGCLSVPGLRGEVPRHTRIRYQGFDPEGTPIDRTV
EGFHARVVQHECDHLIGRLYPSRIRDFSRFGFTDILFPELVRED

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=175, Percent_Identity=30.8571428571429, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1789682, Length=156, Percent_Identity=35.2564102564103, Blast_Score=89, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24645728, Length=177, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24645726, Length=189, Percent_Identity=32.2751322751323, Blast_Score=95, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 22927; Mature: 22927

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCLCSTMGFVDSRRLWCHWPGQFWKVTAMAARPILRMGEPLLFEVAKAVTEFGTSELRAL
CCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHH
IEDMFDSMEAAGGVGLAAPQIGIGLQMVIFGFEKSERYPDAEAVPLTVLINPLITPVGDE
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECHHHCCCCCCCCCCHHEEEHHHHCCCCCC
EELGWEGCLSVPGLRGEVPRHTRIRYQGFDPEGTPIDRTVEGFHARVVQHECDHLIGRLY
HHCCCHHCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
PSRIRDFSRFGFTDILFPELVRED
HHHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure
MCLCSTMGFVDSRRLWCHWPGQFWKVTAMAARPILRMGEPLLFEVAKAVTEFGTSELRAL
CCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHH
IEDMFDSMEAAGGVGLAAPQIGIGLQMVIFGFEKSERYPDAEAVPLTVLINPLITPVGDE
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECHHHCCCCCCCCCCHHEEEHHHHCCCCCC
EELGWEGCLSVPGLRGEVPRHTRIRYQGFDPEGTPIDRTVEGFHARVVQHECDHLIGRLY
HHCCCHHCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
PSRIRDFSRFGFTDILFPELVRED
HHHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]