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The map label for this gene is pyk [H]

Identifier: 71907743

GI number: 71907743

Start: 2287039

End: 2288457

Strand: Reverse

Name: pyk [H]

Synonym: Daro_2117

Alternate gene names: 71907743

Gene position: 2288457-2287039 (Counterclockwise)

Preceding gene: 71907744

Following gene: 71907742

Centisome position: 50.84

GC content: 57.43

Gene sequence:

>1419_bases
ATGCACCGTAACCGTAATGCCAAAATTGTCGCCACCCTCGGCCCAGCCAGTTCTGATCGCGACACGATCCAGAAGCTTTA
CTTGAATGGGGCCGATATCTTTCGTCTTAACTTCAGCCACGGTACCCATGAGGATCACAAAAGAAGCCTGGAAACCATCC
GTGATGTCGAGCGAGAAGCCGGTCGTCCAATTGGCGTGCTCCTTGATCTTCAGGGCCCAAAGCTGCGCCTCGGCAAATTT
GCCAATGGCCCCATCACTTTGGCCGAAGGCGACAAGTTCCGCCTCGACCTGGACTGTGACAGGCCGGGCGATCAAACCCG
GGCCCCCATGCCGCACCCGGAGATTTTCAAGGCGCTGAGCGTACAGTCCAGCCTCCTGATTGACGATGGCCGCATTCGCC
TGTTCGTCGAAAAATGCGGGCCCGACTTCGCCGAAACCAGAGTTGTTGCCGGGGGAACACTATCTGACCGCAAGGGCGTC
AATGTCCCCGGATCACTTATTCCGATGTCGGCAATGACCGACAAGGATCGCGCGGACCTCGATTTTGGTTTAACCCTGGG
AGTAGATTGGGTTGCGCTCTCCTTTGTGCAACGAGCCGAGGACATACTGGAAATCAAGAAAATCATCGATGGCCGTGCCG
GTGTTATCGCCAAACTGGAGAAGCCAGCGGCAATCGAGCGTCTCGAGGCCATCGTCAATGTCAGCGACGCCATCATGGTT
GCTCGTGGCGACCTGGGCGTCGAAATGCCACCGGAACAAGTGCCAGCGATTCAAAAGCGGATCATTCGTGCCTGCAGAAG
CGCCGGCAAGCCGGTTATTGTGGCAACGCAGATGCTTGATTCCATGGTCGCTGCACCGGTGCCGACCCGCGCCGAAGCAT
CCGATGTAGCAACAGCGATCTACGATGGTGCCGATGCCGTCATGCTTTCCGCTGAATCGGCCTCTGGAAAATATCCAGTT
GCAGCAGTAAAGATGATGGACAGCATCATCAGCAGAACCGAGTCCGATCCCTACTATCGGGTAGCCATCGACGCGTCACA
ACCACCGGCACGGGCCAACAAGGCGGATGCCATCGGTTCTGCTGTACGCCATGTCGCCGATCTGCTGCATATTGCCGTGA
CCGTTGCCTATACCAGTTCGGGCACGTCTGCATTGAGCATGGCGCGAGAACGACCCAAGGCCCCGATCATCGCCCTGACG
CCACGACTGGCCACGGCCCGTCGACTGTCTCTGGCCTGGGGTTTGCATGCCGTCATGTGCCATGACGTTGTCGATGTGAC
TGAAATGACTGACCTGGCCTGCGAAACGGCCAGAAGGCAGCATTTTGCTTCACGAGGGCAAAGCGTTGTGATCTCGGCTG
GCATGCCTTTCGGTAGGTCTGGCACGACCAATTTGCTGCGCATCGCCGAACTTAGCTGA

Upstream 100 bases:

>100_bases
CGATCATTACTGGCCCGACGCTGACCAACCTCAATGACTTTCGCGCCATTTTCATTACCGGCGAGACGGCTTGAGTCGCT
CGCTTGAAAGGACACTGAAC

Downstream 100 bases:

>100_bases
TCAGCTGATTAACCATGGCAAGGACTAAGCGGCAGTCGCAGCGCGTACGGGAAAATCACACTATCGCACGGGCGGCAAAT
TGGACAGAATCACCAATAAT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MHRNRNAKIVATLGPASSDRDTIQKLYLNGADIFRLNFSHGTHEDHKRSLETIRDVEREAGRPIGVLLDLQGPKLRLGKF
ANGPITLAEGDKFRLDLDCDRPGDQTRAPMPHPEIFKALSVQSSLLIDDGRIRLFVEKCGPDFAETRVVAGGTLSDRKGV
NVPGSLIPMSAMTDKDRADLDFGLTLGVDWVALSFVQRAEDILEIKKIIDGRAGVIAKLEKPAAIERLEAIVNVSDAIMV
ARGDLGVEMPPEQVPAIQKRIIRACRSAGKPVIVATQMLDSMVAAPVPTRAEASDVATAIYDGADAVMLSAESASGKYPV
AAVKMMDSIISRTESDPYYRVAIDASQPPARANKADAIGSAVRHVADLLHIAVTVAYTSSGTSALSMARERPKAPIIALT
PRLATARRLSLAWGLHAVMCHDVVDVTEMTDLACETARRQHFASRGQSVVISAGMPFGRSGTTNLLRIAELS

Sequences:

>Translated_472_residues
MHRNRNAKIVATLGPASSDRDTIQKLYLNGADIFRLNFSHGTHEDHKRSLETIRDVEREAGRPIGVLLDLQGPKLRLGKF
ANGPITLAEGDKFRLDLDCDRPGDQTRAPMPHPEIFKALSVQSSLLIDDGRIRLFVEKCGPDFAETRVVAGGTLSDRKGV
NVPGSLIPMSAMTDKDRADLDFGLTLGVDWVALSFVQRAEDILEIKKIIDGRAGVIAKLEKPAAIERLEAIVNVSDAIMV
ARGDLGVEMPPEQVPAIQKRIIRACRSAGKPVIVATQMLDSMVAAPVPTRAEASDVATAIYDGADAVMLSAESASGKYPV
AAVKMMDSIISRTESDPYYRVAIDASQPPARANKADAIGSAVRHVADLLHIAVTVAYTSSGTSALSMARERPKAPIIALT
PRLATARRLSLAWGLHAVMCHDVVDVTEMTDLACETARRQHFASRGQSVVISAGMPFGRSGTTNLLRIAELS
>Mature_472_residues
MHRNRNAKIVATLGPASSDRDTIQKLYLNGADIFRLNFSHGTHEDHKRSLETIRDVEREAGRPIGVLLDLQGPKLRLGKF
ANGPITLAEGDKFRLDLDCDRPGDQTRAPMPHPEIFKALSVQSSLLIDDGRIRLFVEKCGPDFAETRVVAGGTLSDRKGV
NVPGSLIPMSAMTDKDRADLDFGLTLGVDWVALSFVQRAEDILEIKKIIDGRAGVIAKLEKPAAIERLEAIVNVSDAIMV
ARGDLGVEMPPEQVPAIQKRIIRACRSAGKPVIVATQMLDSMVAAPVPTRAEASDVATAIYDGADAVMLSAESASGKYPV
AAVKMMDSIISRTESDPYYRVAIDASQPPARANKADAIGSAVRHVADLLHIAVTVAYTSSGTSALSMARERPKAPIIALT
PRLATARRLSLAWGLHAVMCHDVVDVTEMTDLACETARRQHFASRGQSVVISAGMPFGRSGTTNLLRIAELS

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=489, Percent_Identity=38.6503067484663, Blast_Score=313, Evalue=3e-85,
Organism=Homo sapiens, GI33286420, Length=489, Percent_Identity=38.6503067484663, Blast_Score=313, Evalue=3e-85,
Organism=Homo sapiens, GI33286418, Length=493, Percent_Identity=38.9452332657201, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI10835121, Length=498, Percent_Identity=37.14859437751, Blast_Score=304, Evalue=1e-82,
Organism=Homo sapiens, GI32967597, Length=498, Percent_Identity=37.14859437751, Blast_Score=304, Evalue=1e-82,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=38.1944444444444, Blast_Score=195, Evalue=6e-50,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=38.1944444444444, Blast_Score=195, Evalue=6e-50,
Organism=Homo sapiens, GI310128736, Length=238, Percent_Identity=34.453781512605, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI310128734, Length=238, Percent_Identity=34.453781512605, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI310128738, Length=212, Percent_Identity=33.9622641509434, Blast_Score=125, Evalue=1e-28,
Organism=Escherichia coli, GI1787965, Length=467, Percent_Identity=37.2591006423983, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1788160, Length=477, Percent_Identity=35.2201257861635, Blast_Score=263, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI71984413, Length=487, Percent_Identity=38.8090349075975, Blast_Score=293, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI17506829, Length=487, Percent_Identity=38.8090349075975, Blast_Score=293, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI71984406, Length=487, Percent_Identity=38.8090349075975, Blast_Score=292, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17506831, Length=487, Percent_Identity=38.8090349075975, Blast_Score=291, Evalue=4e-79,
Organism=Caenorhabditis elegans, GI17544584, Length=488, Percent_Identity=37.0901639344262, Blast_Score=291, Evalue=7e-79,
Organism=Saccharomyces cerevisiae, GI6319279, Length=484, Percent_Identity=32.6446280991736, Blast_Score=237, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6324923, Length=484, Percent_Identity=33.4710743801653, Blast_Score=231, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24648964, Length=486, Percent_Identity=35.8024691358025, Blast_Score=278, Evalue=5e-75,
Organism=Drosophila melanogaster, GI28571814, Length=486, Percent_Identity=35.8024691358025, Blast_Score=278, Evalue=6e-75,
Organism=Drosophila melanogaster, GI24648966, Length=412, Percent_Identity=35.6796116504854, Blast_Score=240, Evalue=2e-63,
Organism=Drosophila melanogaster, GI24581235, Length=484, Percent_Identity=31.404958677686, Blast_Score=239, Evalue=3e-63,
Organism=Drosophila melanogaster, GI24646914, Length=280, Percent_Identity=36.4285714285714, Blast_Score=164, Evalue=8e-41,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50834; Mature: 50834

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRNRNAKIVATLGPASSDRDTIQKLYLNGADIFRLNFSHGTHEDHKRSLETIRDVEREA
CCCCCCCEEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
GRPIGVLLDLQGPKLRLGKFANGPITLAEGDKFRLDLDCDRPGDQTRAPMPHPEIFKALS
CCCEEEEEECCCCCEEECCCCCCCEEEECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHH
VQSSLLIDDGRIRLFVEKCGPDFAETRVVAGGTLSDRKGVNVPGSLIPMSAMTDKDRADL
CCCCEEEECCCEEEEEECCCCCHHHCEEEECCCCCCCCCCCCCCCEEECHHCCCCCCCCC
DFGLTLGVDWVALSFVQRAEDILEIKKIIDGRAGVIAKLEKPAAIERLEAIVNVSDAIMV
CCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCEEEE
ARGDLGVEMPPEQVPAIQKRIIRACRSAGKPVIVATQMLDSMVAAPVPTRAEASDVATAI
EECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCHHHHHHHH
YDGADAVMLSAESASGKYPVAAVKMMDSIISRTESDPYYRVAIDASQPPARANKADAIGS
HCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHH
AVRHVADLLHIAVTVAYTSSGTSALSMARERPKAPIIALTPRLATARRLSLAWGLHAVMC
HHHHHHHHHHHHHEEEECCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
HDVVDVTEMTDLACETARRQHFASRGQSVVISAGMPFGRSGTTNLLRIAELS
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MHRNRNAKIVATLGPASSDRDTIQKLYLNGADIFRLNFSHGTHEDHKRSLETIRDVEREA
CCCCCCCEEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
GRPIGVLLDLQGPKLRLGKFANGPITLAEGDKFRLDLDCDRPGDQTRAPMPHPEIFKALS
CCCEEEEEECCCCCEEECCCCCCCEEEECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHH
VQSSLLIDDGRIRLFVEKCGPDFAETRVVAGGTLSDRKGVNVPGSLIPMSAMTDKDRADL
CCCCEEEECCCEEEEEECCCCCHHHCEEEECCCCCCCCCCCCCCCEEECHHCCCCCCCCC
DFGLTLGVDWVALSFVQRAEDILEIKKIIDGRAGVIAKLEKPAAIERLEAIVNVSDAIMV
CCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCEEEE
ARGDLGVEMPPEQVPAIQKRIIRACRSAGKPVIVATQMLDSMVAAPVPTRAEASDVATAI
EECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCHHHHHHHH
YDGADAVMLSAESASGKYPVAAVKMMDSIISRTESDPYYRVAIDASQPPARANKADAIGS
HCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHH
AVRHVADLLHIAVTVAYTSSGTSALSMARERPKAPIIALTPRLATARRLSLAWGLHAVMC
HHHHHHHHHHHHHEEEECCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
HDVVDVTEMTDLACETARRQHFASRGQSVVISAGMPFGRSGTTNLLRIAELS
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA