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The map label for this gene is phnE [H]

Identifier: 71907453

GI number: 71907453

Start: 1961565

End: 1963121

Strand: Reverse

Name: phnE [H]

Synonym: Daro_1824

Alternate gene names: 71907453

Gene position: 1963121-1961565 (Counterclockwise)

Preceding gene: 71907454

Following gene: 71907452

Centisome position: 43.61

GC content: 65.38

Gene sequence:

>1557_bases
ATGCTGCTGAATCCAGCCGACCGCGACCCTGCTGCTCGTGGCCGCCTGATCGGCGCACTGGCAACGCTCGTCATCCTCTG
TCCATTATTCCAGGCCGCCGAGGTCAAGCCGGGCGCCCTGTTCGAAAGCGGCAACCTGACGGTCATCGGCAACTTTCTTA
CGGCCTTCCTGCCACCGGAAACCGGTGCCGAATTTCTTGGCTATCTCGCCAAGGCGACGCTGGAAACACTGGCCATCGCC
ACCGCCGGCATGGCACTCGCCTTCCTGATTGCCGTGCCGATGGGTTATCTCGCCACTGGCGCCGGCCGTGAAAAACCAAC
CCTCACCCCGATCACCCGCAGCCTGCTGACCATCCTGCGCGGCATTCCCGAACTGGTCTGGGCGCTTGTTTTCGTCCGCG
TCTTTGGTCTTGGCCCGGCGGCCGGCGTGCTGGCGCTGGGGCTGACCTACGGCGGCATGCTGGCCAAGGTTTATGCCGAA
ATCCTCGAATCGGTCGATGCTGCCCCGGCCCTGGCGCTGCGCCACAGCGGTGCCAGCCGGCCGCTGGCCATTCTCTACGG
CCTGATTCCGCAGGCCTCGAAGGAACTCGCGTCCTACACGGTCTATCGCTGGGAGTGCGCCATCCGTGCCTCGGTGGTCA
TGGGCTTCGTCGGTGCCGGCGGCCTCGGCCAGTTGATGGATCAGGCCATGAAGATGCTCAATGGCGGCGAAGCGGCAACC
ATCCTGCTCACCTTCATGGGGCTCGTCTTCTCGGCCGACGGCCTGTCAGGCTGGCTGCGCCGGGCGCTCGAGATGCCGCC
AGCGAATCATGCCTCCCCTTTCGGCTGGCGCAGCATTGGCCTGCTCGTCACGCTGTTCGCTTCAATCGTTGCCAGCTTCC
AACTGCTCGACATCGGCTTTGGCGAACTTTTTTCAGCCGAAGCAGCCCATTCCATCGGCGAATTCATCGCCGGTTTCTTC
CCGCCCGACCTTTCCGCAGAGTGGCTGGCCAAGGTCGCCAAGGGCATCTGGGAAACACTGGCCATCTCGGTCGTTGGCAC
GCTGCTGGCGGCCATCGCCGGCCTGCTGTTGGCGCTGCCGAAATGGCGCGCCCCGTTCAGCTTCATCCTCAACTCACTGC
GCTCGGTGCCGGAGCTGGTCTGGGCAACCATCACCGCCCTCGCCGTCGGCCTCGGCCCCTTTGCCGGTGCGCTGGCGCTG
GCCCTGCATACGGCCGGCGTGCTTGGCCGCCTCTACGCCGAAGCGCTCGACAATGCGCCGCCCACACCCGGCAAGGCGCT
GCGCCTGGCCGGCGCACCGGGCGGCTTCGCCTTCCTTTACGGCACGCTGCCCGGCGCGGCACCGCAGCTGATCGCCTATA
CGCTGTACCGCTGGGAAATGAATATCCGGATGGCCGCCATCCTTGGCTTTGTCGGCGCCGGCGGCCTTGGCCAGCTGCTC
TATTTCGAGCTTTCGCTGTTCCATCATGCGCAAGCCTCGACCGTGATCATGGCCATGCTGTTGCTATCGATTGCCGTCGA
TCAGGCCAGCGCCTGGCTGCGCCAGCGGATGCGCTGA

Upstream 100 bases:

>100_bases
ATGAGCTGTACGCCAGCGAAGGCGAGCAACTGCCCATCCAGGCGCACGAACCGCATTTCCTGTCGCAAGTACCTGCCAAT
GATGCAGCGACGCGGGCGAA

Downstream 100 bases:

>100_bases
CCGCTCGCCCCACCCGGCAGACCGGCGTATATTTCGCCCCTTGCCGCGACCGCCACCCGGCGCCCTAACCTGCTCCTGCT
CCAAGACCATGCCGCTCCCG

Product: phosphonate ABC transporter inner membrane protein

Products: 3-phospho-serine [Cytoplasm]; phosphite [Cytoplasm]; ADP; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MLLNPADRDPAARGRLIGALATLVILCPLFQAAEVKPGALFESGNLTVIGNFLTAFLPPETGAEFLGYLAKATLETLAIA
TAGMALAFLIAVPMGYLATGAGREKPTLTPITRSLLTILRGIPELVWALVFVRVFGLGPAAGVLALGLTYGGMLAKVYAE
ILESVDAAPALALRHSGASRPLAILYGLIPQASKELASYTVYRWECAIRASVVMGFVGAGGLGQLMDQAMKMLNGGEAAT
ILLTFMGLVFSADGLSGWLRRALEMPPANHASPFGWRSIGLLVTLFASIVASFQLLDIGFGELFSAEAAHSIGEFIAGFF
PPDLSAEWLAKVAKGIWETLAISVVGTLLAAIAGLLLALPKWRAPFSFILNSLRSVPELVWATITALAVGLGPFAGALAL
ALHTAGVLGRLYAEALDNAPPTPGKALRLAGAPGGFAFLYGTLPGAAPQLIAYTLYRWEMNIRMAAILGFVGAGGLGQLL
YFELSLFHHAQASTVIMAMLLLSIAVDQASAWLRQRMR

Sequences:

>Translated_518_residues
MLLNPADRDPAARGRLIGALATLVILCPLFQAAEVKPGALFESGNLTVIGNFLTAFLPPETGAEFLGYLAKATLETLAIA
TAGMALAFLIAVPMGYLATGAGREKPTLTPITRSLLTILRGIPELVWALVFVRVFGLGPAAGVLALGLTYGGMLAKVYAE
ILESVDAAPALALRHSGASRPLAILYGLIPQASKELASYTVYRWECAIRASVVMGFVGAGGLGQLMDQAMKMLNGGEAAT
ILLTFMGLVFSADGLSGWLRRALEMPPANHASPFGWRSIGLLVTLFASIVASFQLLDIGFGELFSAEAAHSIGEFIAGFF
PPDLSAEWLAKVAKGIWETLAISVVGTLLAAIAGLLLALPKWRAPFSFILNSLRSVPELVWATITALAVGLGPFAGALAL
ALHTAGVLGRLYAEALDNAPPTPGKALRLAGAPGGFAFLYGTLPGAAPQLIAYTLYRWEMNIRMAAILGFVGAGGLGQLL
YFELSLFHHAQASTVIMAMLLLSIAVDQASAWLRQRMR
>Mature_518_residues
MLLNPADRDPAARGRLIGALATLVILCPLFQAAEVKPGALFESGNLTVIGNFLTAFLPPETGAEFLGYLAKATLETLAIA
TAGMALAFLIAVPMGYLATGAGREKPTLTPITRSLLTILRGIPELVWALVFVRVFGLGPAAGVLALGLTYGGMLAKVYAE
ILESVDAAPALALRHSGASRPLAILYGLIPQASKELASYTVYRWECAIRASVVMGFVGAGGLGQLMDQAMKMLNGGEAAT
ILLTFMGLVFSADGLSGWLRRALEMPPANHASPFGWRSIGLLVTLFASIVASFQLLDIGFGELFSAEAAHSIGEFIAGFF
PPDLSAEWLAKVAKGIWETLAISVVGTLLAAIAGLLLALPKWRAPFSFILNSLRSVPELVWATITALAVGLGPFAGALAL
ALHTAGVLGRLYAEALDNAPPTPGKALRLAGAPGGFAFLYGTLPGAAPQLIAYTLYRWEMNIRMAAILGFVGAGGLGQLL
YFELSLFHHAQASTVIMAMLLLSIAVDQASAWLRQRMR

Specific function: Part of the ABC transporter complex phnCDE involved in phosphate import. Responsible for the translocation of the substrate across the membrane [H]

COG id: COG3639

COG function: function code P; ABC-type phosphate/phosphonate transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005769 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 54416; Mature: 54416

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLNPADRDPAARGRLIGALATLVILCPLFQAAEVKPGALFESGNLTVIGNFLTAFLPPE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEHHHHHHHCCCC
TGAEFLGYLAKATLETLAIATAGMALAFLIAVPMGYLATGAGREKPTLTPITRSLLTILR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
GIPELVWALVFVRVFGLGPAAGVLALGLTYGGMLAKVYAEILESVDAAPALALRHSGASR
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCC
PLAILYGLIPQASKELASYTVYRWECAIRASVVMGFVGAGGLGQLMDQAMKMLNGGEAAT
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHH
ILLTFMGLVFSADGLSGWLRRALEMPPANHASPFGWRSIGLLVTLFASIVASFQLLDIGF
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCH
GELFSAEAAHSIGEFIAGFFPPDLSAEWLAKVAKGIWETLAISVVGTLLAAIAGLLLALP
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KWRAPFSFILNSLRSVPELVWATITALAVGLGPFAGALALALHTAGVLGRLYAEALDNAP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PTPGKALRLAGAPGGFAFLYGTLPGAAPQLIAYTLYRWEMNIRMAAILGFVGAGGLGQLL
CCCCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
YFELSLFHHAQASTVIMAMLLLSIAVDQASAWLRQRMR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLLNPADRDPAARGRLIGALATLVILCPLFQAAEVKPGALFESGNLTVIGNFLTAFLPPE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEHHHHHHHCCCC
TGAEFLGYLAKATLETLAIATAGMALAFLIAVPMGYLATGAGREKPTLTPITRSLLTILR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
GIPELVWALVFVRVFGLGPAAGVLALGLTYGGMLAKVYAEILESVDAAPALALRHSGASR
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCC
PLAILYGLIPQASKELASYTVYRWECAIRASVVMGFVGAGGLGQLMDQAMKMLNGGEAAT
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHH
ILLTFMGLVFSADGLSGWLRRALEMPPANHASPFGWRSIGLLVTLFASIVASFQLLDIGF
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCH
GELFSAEAAHSIGEFIAGFFPPDLSAEWLAKVAKGIWETLAISVVGTLLAAIAGLLLALP
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KWRAPFSFILNSLRSVPELVWATITALAVGLGPFAGALALALHTAGVLGRLYAEALDNAP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PTPGKALRLAGAPGGFAFLYGTLPGAAPQLIAYTLYRWEMNIRMAAILGFVGAGGLGQLL
CCCCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
YFELSLFHHAQASTVIMAMLLLSIAVDQASAWLRQRMR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA