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The map label for this gene is fdoH [H]

Identifier: 71907446

GI number: 71907446

Start: 1952554

End: 1953441

Strand: Reverse

Name: fdoH [H]

Synonym: Daro_1817

Alternate gene names: 71907446

Gene position: 1953441-1952554 (Counterclockwise)

Preceding gene: 71907447

Following gene: 71907445

Centisome position: 43.4

GC content: 61.94

Gene sequence:

>888_bases
ATGGCACTGCAATCGCTAGACATCAAACAGCGCTCCGCGACGACGACACCCTCGACGCAGGCACGCCAGACCATCGAGAT
CGCCAAGTTGATCGACGTATCGACCTGCATCGGCTGCAAGGCCTGCCAGTCGGCGTGCATGGAATGGAACGACCTGCGGC
CGGAAATCGGCAACAACGTCGGTGTCCACGACAATCCGACCGATCTCGGGCCGGAATCGTGGACCGTCATGCGCTTCTCG
GAAGTGGAGCAGAACAACACGCTGGAGTGGCTGATCCGCAAGGACGGCTGCATGCACTGTTCCGACCCCGGCTGCCTGAA
GGCCTGTCCATCTCCCGGCGCGATCATCCAGTACAGCAACGGCATTGTCGATTTCCATCAGGAAAACTGCATTGGCTGCG
GTTACTGCGTGACCGGCTGCCCCTTCAACGTCCCCCGCATCTCCCAGAAAGACAGCAAGGCCTACAAGTGCTCGCTCTGT
TCCGACCGGGTGGCGGCCAACCAGGCGCCGGCCTGTGCCAAGGCCTGCCCGACTGGCGCGATCCAGTTCGGTAGCAAGGA
GGACATGAAGGACTTCGCCGAAACGCGGATCGCCGACCTCAAGGAACGCGGTTTCGACAAGGCCGGCCTGTACGATCCGG
AAGGCGTCGGCGGTACGCACGTCATGTACGTACTGCACCATGCCGACCAGCCGGGGCTGTACCACGGCCTGCCGGCCAAC
CCGTCGATCAGCCCGCTGGTCTCGTTGTGGAAGGGCTTCGCCAAGCCGCTCGCCACGCTGGCTTTGGCCGGTGTTGCACT
GGGTAGCCTGTTCCATTACGTGACCAAGGGTCCGAACGAGGTCTCGAAAGAGATCGAGGATGAGATCGAGAAGGAGGACG
CACAATGA

Upstream 100 bases:

>100_bases
TGTCCAACACCCTGACGCCCTTCGTCGGTGACGCGAACTCGCAAACACCGGAGTTCAAGTCCTTCCTCGTCAACATCGAG
AAGATCTAAGGAGAGCTGCC

Downstream 100 bases:

>100_bases
TCCGCGATCCGAAAGACCTCAAGCGCTACGAGCCCAAGGAACGGGCCAATCACTGGGTGGTGGGCATCAGCTTCATCCTG
CTCGCCCTCTCCGGTCTGGC

Product: formate dehydrogenase beta subunit

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase iron-sulfur subunit; FDH-Z subunit beta; Formate dehydrogenase-O subunit beta [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MALQSLDIKQRSATTTPSTQARQTIEIAKLIDVSTCIGCKACQSACMEWNDLRPEIGNNVGVHDNPTDLGPESWTVMRFS
EVEQNNTLEWLIRKDGCMHCSDPGCLKACPSPGAIIQYSNGIVDFHQENCIGCGYCVTGCPFNVPRISQKDSKAYKCSLC
SDRVAANQAPACAKACPTGAIQFGSKEDMKDFAETRIADLKERGFDKAGLYDPEGVGGTHVMYVLHHADQPGLYHGLPAN
PSISPLVSLWKGFAKPLATLALAGVALGSLFHYVTKGPNEVSKEIEDEIEKEDAQ

Sequences:

>Translated_295_residues
MALQSLDIKQRSATTTPSTQARQTIEIAKLIDVSTCIGCKACQSACMEWNDLRPEIGNNVGVHDNPTDLGPESWTVMRFS
EVEQNNTLEWLIRKDGCMHCSDPGCLKACPSPGAIIQYSNGIVDFHQENCIGCGYCVTGCPFNVPRISQKDSKAYKCSLC
SDRVAANQAPACAKACPTGAIQFGSKEDMKDFAETRIADLKERGFDKAGLYDPEGVGGTHVMYVLHHADQPGLYHGLPAN
PSISPLVSLWKGFAKPLATLALAGVALGSLFHYVTKGPNEVSKEIEDEIEKEDAQ
>Mature_294_residues
ALQSLDIKQRSATTTPSTQARQTIEIAKLIDVSTCIGCKACQSACMEWNDLRPEIGNNVGVHDNPTDLGPESWTVMRFSE
VEQNNTLEWLIRKDGCMHCSDPGCLKACPSPGAIIQYSNGIVDFHQENCIGCGYCVTGCPFNVPRISQKDSKAYKCSLCS
DRVAANQAPACAKACPTGAIQFGSKEDMKDFAETRIADLKERGFDKAGLYDPEGVGGTHVMYVLHHADQPGLYHGLPANP
SISPLVSLWKGFAKPLATLALAGVALGSLFHYVTKGPNEVSKEIEDEIEKEDAQ

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790326, Length=298, Percent_Identity=65.7718120805369, Blast_Score=424, Evalue=1e-120,
Organism=Escherichia coli, GI1787749, Length=293, Percent_Identity=65.5290102389078, Blast_Score=415, Evalue=1e-117,
Organism=Escherichia coli, GI1787122, Length=167, Percent_Identity=32.9341317365269, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787872, Length=167, Percent_Identity=32.9341317365269, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789370, Length=273, Percent_Identity=29.3040293040293, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI2367345, Length=158, Percent_Identity=32.9113924050633, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI226510944, Length=177, Percent_Identity=28.2485875706215, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788811, Length=169, Percent_Identity=30.7692307692308, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787478, Length=212, Percent_Identity=26.8867924528302, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787963, Length=154, Percent_Identity=25.974025974026, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87082180, Length=188, Percent_Identity=27.6595744680851, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787740, Length=100, Percent_Identity=32, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI2367245, Length=165, Percent_Identity=30.3030303030303, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87082179, Length=173, Percent_Identity=26.0115606936416, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789067, Length=183, Percent_Identity=28.4153005464481, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 31941; Mature: 31810

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
5.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALQSLDIKQRSATTTPSTQARQTIEIAKLIDVSTCIGCKACQSACMEWNDLRPEIGNNV
CCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCHHCCCHHHCCCC
GVHDNPTDLGPESWTVMRFSEVEQNNTLEWLIRKDGCMHCSDPGCLKACPSPGAIIQYSN
CCCCCCCCCCCCCEEEEEEHHHCCCCCEEEEEECCCCCCCCCCCHHCCCCCCCCEEEECC
GIVDFHQENCIGCGYCVTGCPFNVPRISQKDSKAYKCSLCSDRVAANQAPACAKACPTGA
CEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCCHHHHHCCCCC
IQFGSKEDMKDFAETRIADLKERGFDKAGLYDPEGVGGTHVMYVLHHADQPGLYHGLPAN
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCC
PSISPLVSLWKGFAKPLATLALAGVALGSLFHYVTKGPNEVSKEIEDEIEKEDAQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALQSLDIKQRSATTTPSTQARQTIEIAKLIDVSTCIGCKACQSACMEWNDLRPEIGNNV
CCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCHHCCCHHHCCCC
GVHDNPTDLGPESWTVMRFSEVEQNNTLEWLIRKDGCMHCSDPGCLKACPSPGAIIQYSN
CCCCCCCCCCCCCEEEEEEHHHCCCCCEEEEEECCCCCCCCCCCHHCCCCCCCCEEEECC
GIVDFHQENCIGCGYCVTGCPFNVPRISQKDSKAYKCSLCSDRVAANQAPACAKACPTGA
CEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCCHHHHHCCCCC
IQFGSKEDMKDFAETRIADLKERGFDKAGLYDPEGVGGTHVMYVLHHADQPGLYHGLPAN
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCC
PSISPLVSLWKGFAKPLATLALAGVALGSLFHYVTKGPNEVSKEIEDEIEKEDAQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]