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The map label for this gene is fdoI [H]

Identifier: 71907445

GI number: 71907445

Start: 1951937

End: 1952557

Strand: Reverse

Name: fdoI [H]

Synonym: Daro_1816

Alternate gene names: 71907445

Gene position: 1952557-1951937 (Counterclockwise)

Preceding gene: 71907446

Following gene: 71907444

Centisome position: 43.38

GC content: 62.0

Gene sequence:

>621_bases
ATGATCCGCGATCCGAAAGACCTCAAGCGCTACGAGCCCAAGGAACGGGCCAATCACTGGGTGGTGGGCATCAGCTTCAT
CCTGCTCGCCCTCTCCGGTCTGGCCTTCTTCCACCCGGCCTTCTTCCCGCTGACGCAGCTGTTCGGCGGCCCTGTCTGGG
CGCGCATCATCCACCCCTACGTCGGGGTGCTGATGGCGGTGTCCTTCCTCGGCCTCTTTTTCCGCTTCAAGCATCTGAAC
AAGATGACGCCGGCGGACAAAGAGTGGTTGTCGAAGGCCAAGCAGATGGTGGACGGCGACGACCACAACATGCCGGAACA
AGGCAAGTACAACGGTGGCCAGAAAGTCATGTTCTGGGTCATGGCGACCTGCATGCTGCTGATGACGGTCTCTGGCCTGC
TCATCTGGCGCTCCTGGTTCGGTTTCGACATCACGCTCGTCCGCCTGGGCGCCGTGGTGCATGCCGCAGCCGGCGCCGTG
ATGATCGGCATGATCTTCGTCCACGTCTATGCCGCCATCTGGGTCAAGGGCACCATCCGCGCCATGTGGTACGGCACGGT
GACCCGGGGTTGGGCCAAGCAGCATCACCGTGGCTGGTACCGTCAGGTGACCGGCAAATAA

Upstream 100 bases:

>100_bases
TGGCCGGTGTTGCACTGGGTAGCCTGTTCCATTACGTGACCAAGGGTCCGAACGAGGTCTCGAAAGAGATCGAGGATGAG
ATCGAGAAGGAGGACGCACA

Downstream 100 bases:

>100_bases
GTTATTAGCTTTACTTGCGTTCAGAGGGGCTCTGCGGGGAATATTCCGCATCCCCTTCTTTTTTCAAAAACAACTCGATA
GCCCACCGCAAGATGTCGCC

Product: formate dehydrogenase gamma subunit

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase cytochrome b556 subunit; FDH-Z subunit gamma; Formate dehydrogenase-O subunit gamma [H]

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MIRDPKDLKRYEPKERANHWVVGISFILLALSGLAFFHPAFFPLTQLFGGPVWARIIHPYVGVLMAVSFLGLFFRFKHLN
KMTPADKEWLSKAKQMVDGDDHNMPEQGKYNGGQKVMFWVMATCMLLMTVSGLLIWRSWFGFDITLVRLGAVVHAAAGAV
MIGMIFVHVYAAIWVKGTIRAMWYGTVTRGWAKQHHRGWYRQVTGK

Sequences:

>Translated_206_residues
MIRDPKDLKRYEPKERANHWVVGISFILLALSGLAFFHPAFFPLTQLFGGPVWARIIHPYVGVLMAVSFLGLFFRFKHLN
KMTPADKEWLSKAKQMVDGDDHNMPEQGKYNGGQKVMFWVMATCMLLMTVSGLLIWRSWFGFDITLVRLGAVVHAAAGAV
MIGMIFVHVYAAIWVKGTIRAMWYGTVTRGWAKQHHRGWYRQVTGK
>Mature_206_residues
MIRDPKDLKRYEPKERANHWVVGISFILLALSGLAFFHPAFFPLTQLFGGPVWARIIHPYVGVLMAVSFLGLFFRFKHLN
KMTPADKEWLSKAKQMVDGDDHNMPEQGKYNGGQKVMFWVMATCMLLMTVSGLLIWRSWFGFDITLVRLGAVVHAAAGAV
MIGMIFVHVYAAIWVKGTIRAMWYGTVTRGWAKQHHRGWYRQVTGK

Specific function: Allows to use formate as major electron donor during aerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1790325, Length=196, Percent_Identity=44.8979591836735, Blast_Score=174, Evalue=3e-45,
Organism=Escherichia coli, GI1787750, Length=200, Percent_Identity=42.5, Blast_Score=165, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 23558; Mature: 23558

Theoretical pI: Translated: 10.73; Mature: 10.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRDPKDLKRYEPKERANHWVVGISFILLALSGLAFFHPAFFPLTQLFGGPVWARIIHPY
CCCCCHHHHHCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
VGVLMAVSFLGLFFRFKHLNKMTPADKEWLSKAKQMVDGDDHNMPEQGKYNGGQKVMFWV
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
MATCMLLMTVSGLLIWRSWFGFDITLVRLGAVVHAAAGAVMIGMIFVHVYAAIWVKGTIR
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMWYGTVTRGWAKQHHRGWYRQVTGK
HHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIRDPKDLKRYEPKERANHWVVGISFILLALSGLAFFHPAFFPLTQLFGGPVWARIIHPY
CCCCCHHHHHCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
VGVLMAVSFLGLFFRFKHLNKMTPADKEWLSKAKQMVDGDDHNMPEQGKYNGGQKVMFWV
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
MATCMLLMTVSGLLIWRSWFGFDITLVRLGAVVHAAAGAVMIGMIFVHVYAAIWVKGTIR
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMWYGTVTRGWAKQHHRGWYRQVTGK
HHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]