Click here to switch to the map view.

The map label for this gene is pilT [H]

Identifier: 71907355

GI number: 71907355

Start: 1853326

End: 1854513

Strand: Reverse

Name: pilT [H]

Synonym: Daro_1725

Alternate gene names: 71907355

Gene position: 1854513-1853326 (Counterclockwise)

Preceding gene: 71907356

Following gene: 71907354

Centisome position: 41.2

GC content: 57.66

Gene sequence:

>1188_bases
ATGATTCTCGACAAACTGTTCCAGCTGATGGCCGAAAAGCAAGCCTCGGATATTTTCATTTCCGCAGGCGCGCCTATCCA
TATCAAGATTCAGGGCAACACGATTCCGGTCAATCAGCAGGTGATGCTGCCTGACATGATCGAGAAGATCGCCTACGAAC
TGATGTCCCCGGATCAGATCAAGACCTTCGAGGCAACGATGGAGATGAATCTCTCCTTCGGTGTGCCCAATGTCGGTAAC
TTCCGCGTCAATATTTTCCGCCAGCGCGGCTCGACCAGCATCGTCATCCGCTTCATCCTGGGCAATATTCCGGCCATGGA
TGAACTCGGCCTGCCGAACATCCTCGGCGAGCTGATCATGGAAAAGCGCGGCCTGATCCTGATCGTTGGCGCCACCGGCT
CGGGTAAATCGACGACCCTCGCCTCGATGCTCGATCACCGCAATGCCAACCGTTCGGGCCACATCCTGACCGTCGAAGAC
CCGATCGAATTCCTGTTCAAGCACAAGAAATCCATCGTCAACCAGCGCGAAATCGGCATGGACACGATGGACTGGCAGGC
CGCCCTGAAGAACGCCATGCGCCAGGCGCCGGACTGCATCCTGATCGGAGAAATCCGCGACAAGGAAACCATGCAGGCTG
CCATCGCCTACGCGCAAACTGGCCACCTGTGTCTGGCCACACTCCACGCCAACAACAGCTACCACGCGCTGAACCGCATC
ATCAGCTTCTTCCCGCTCGAAAACCGCCCGGCGCTGTTCCTCGACCTGTCGGTCGCCCTGCGCGCCATCATTTCGCAGCG
CCTGGTCAAGAAACCCGATGGCAAGCGCATCCCGACCTGCGAAATCATGCTCAACACCCGCCACATCAGCGAACTGATCG
AGCGCGGTGAAGTCCAAGGCATCAAGGATGCGATGGAACAAACCCTGGCCCCGGGATCGCAGACCTTCGAGCAGGATTTG
TTCAACCTGTACAGCAGCAGCACGATCACCATCGACGAGGCGCTGGCCAATGCCGATTCGCCGACGAACCTGTCGTGGCT
GATCAACAACTCCGAATTCGGCAGCACCAATGGCAAGGACGACAAGAAAACCGATGTCGCTCTCGAGTTCGACAGCACCA
GCGATGGCGGCACTTCCTTCAAGGAATTCACGCTCAGCCTGGCTGACCACGACGAAGAGTCGCACTGA

Upstream 100 bases:

>100_bases
GCAGCCATCATCGTCAAGAAGGTTGATGCGCAGACCCGCTCGAAGACCAGCATCAACGAACTGCTGCGTCCGTAAATCAA
TCCCCCACGAAGGTCTTGTC

Downstream 100 bases:

>100_bases
TGTCCGACACCACGCTCGAACTGGCCAAGCGAATCATCGCCTGCGCCTCCGTGACGCCTGAAGACGCAGGCTGCATGGAC
ATTCTGATCGAACGGCTGAA

Product: pilus retraction protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MILDKLFQLMAEKQASDIFISAGAPIHIKIQGNTIPVNQQVMLPDMIEKIAYELMSPDQIKTFEATMEMNLSFGVPNVGN
FRVNIFRQRGSTSIVIRFILGNIPAMDELGLPNILGELIMEKRGLILIVGATGSGKSTTLASMLDHRNANRSGHILTVED
PIEFLFKHKKSIVNQREIGMDTMDWQAALKNAMRQAPDCILIGEIRDKETMQAAIAYAQTGHLCLATLHANNSYHALNRI
ISFFPLENRPALFLDLSVALRAIISQRLVKKPDGKRIPTCEIMLNTRHISELIERGEVQGIKDAMEQTLAPGSQTFEQDL
FNLYSSSTITIDEALANADSPTNLSWLINNSEFGSTNGKDDKKTDVALEFDSTSDGGTSFKEFTLSLADHDEESH

Sequences:

>Translated_395_residues
MILDKLFQLMAEKQASDIFISAGAPIHIKIQGNTIPVNQQVMLPDMIEKIAYELMSPDQIKTFEATMEMNLSFGVPNVGN
FRVNIFRQRGSTSIVIRFILGNIPAMDELGLPNILGELIMEKRGLILIVGATGSGKSTTLASMLDHRNANRSGHILTVED
PIEFLFKHKKSIVNQREIGMDTMDWQAALKNAMRQAPDCILIGEIRDKETMQAAIAYAQTGHLCLATLHANNSYHALNRI
ISFFPLENRPALFLDLSVALRAIISQRLVKKPDGKRIPTCEIMLNTRHISELIERGEVQGIKDAMEQTLAPGSQTFEQDL
FNLYSSSTITIDEALANADSPTNLSWLINNSEFGSTNGKDDKKTDVALEFDSTSDGGTSFKEFTLSLADHDEESH
>Mature_395_residues
MILDKLFQLMAEKQASDIFISAGAPIHIKIQGNTIPVNQQVMLPDMIEKIAYELMSPDQIKTFEATMEMNLSFGVPNVGN
FRVNIFRQRGSTSIVIRFILGNIPAMDELGLPNILGELIMEKRGLILIVGATGSGKSTTLASMLDHRNANRSGHILTVED
PIEFLFKHKKSIVNQREIGMDTMDWQAALKNAMRQAPDCILIGEIRDKETMQAAIAYAQTGHLCLATLHANNSYHALNRI
ISFFPLENRPALFLDLSVALRAIISQRLVKKPDGKRIPTCEIMLNTRHISELIERGEVQGIKDAMEQTLAPGSQTFEQDL
FNLYSSSTITIDEALANADSPTNLSWLINNSEFGSTNGKDDKKTDVALEFDSTSDGGTSFKEFTLSLADHDEESH

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=321, Percent_Identity=33.9563862928349, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI1786296, Length=290, Percent_Identity=32.7586206896552, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1789723, Length=158, Percent_Identity=43.0379746835443, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 43823; Mature: 43823

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILDKLFQLMAEKQASDIFISAGAPIHIKIQGNTIPVNQQVMLPDMIEKIAYELMSPDQI
CCHHHHHHHHHHCCCCCEEEECCCCEEEEEECCEECCCCCEECHHHHHHHHHHHCCCCHH
KTFEATMEMNLSFGVPNVGNFRVNIFRQRGSTSIVIRFILGNIPAMDELGLPNILGELIM
EEEEEEEEEEEEECCCCCCCEEEEEEEECCCCEEEEEEECCCCCCCHHCCCHHHHHHHHH
EKRGLILIVGATGSGKSTTLASMLDHRNANRSGHILTVEDPIEFLFKHKKSIVNQREIGM
CCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCC
DTMDWQAALKNAMRQAPDCILIGEIRDKETMQAAIAYAQTGHLCLATLHANNSYHALNRI
CCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHH
ISFFPLENRPALFLDLSVALRAIISQRLVKKPDGKRIPTCEIMLNTRHISELIERGEVQG
HHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCEEEEEECHHHHHHHHHCCCCCH
IKDAMEQTLAPGSQTFEQDLFNLYSSSTITIDEALANADSPTNLSWLINNSEFGSTNGKD
HHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEHHHHCCCCCCCCEEEEEECCCCCCCCCCC
DKKTDVALEFDSTSDGGTSFKEFTLSLADHDEESH
CCCCEEEEEECCCCCCCCCHHHHEEEHHCCCCCCC
>Mature Secondary Structure
MILDKLFQLMAEKQASDIFISAGAPIHIKIQGNTIPVNQQVMLPDMIEKIAYELMSPDQI
CCHHHHHHHHHHCCCCCEEEECCCCEEEEEECCEECCCCCEECHHHHHHHHHHHCCCCHH
KTFEATMEMNLSFGVPNVGNFRVNIFRQRGSTSIVIRFILGNIPAMDELGLPNILGELIM
EEEEEEEEEEEEECCCCCCCEEEEEEEECCCCEEEEEEECCCCCCCHHCCCHHHHHHHHH
EKRGLILIVGATGSGKSTTLASMLDHRNANRSGHILTVEDPIEFLFKHKKSIVNQREIGM
CCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCC
DTMDWQAALKNAMRQAPDCILIGEIRDKETMQAAIAYAQTGHLCLATLHANNSYHALNRI
CCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHH
ISFFPLENRPALFLDLSVALRAIISQRLVKKPDGKRIPTCEIMLNTRHISELIERGEVQG
HHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCEEEEEECHHHHHHHHHCCCCCH
IKDAMEQTLAPGSQTFEQDLFNLYSSSTITIDEALANADSPTNLSWLINNSEFGSTNGKD
HHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEHHHHCCCCCCCCEEEEEECCCCCCCCCCC
DKKTDVALEFDSTSDGGTSFKEFTLSLADHDEESH
CCCCEEEEEECCCCCCCCCHHHHEEEHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]