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The map label for this gene is fumB [H]

Identifier: 71907258

GI number: 71907258

Start: 1746729

End: 1748261

Strand: Reverse

Name: fumB [H]

Synonym: Daro_1627

Alternate gene names: 71907258

Gene position: 1748261-1746729 (Counterclockwise)

Preceding gene: 71907259

Following gene: 71907257

Centisome position: 38.84

GC content: 62.62

Gene sequence:

>1533_bases
ATGACCGCCATCAAACAGGAAGACCTGATCCAGTCCGTCGCCGATGCCTTCCAGTACATCAGCTACTATCACCCGCTCGA
TTACATCAAGGCGCTGGGTGAAGCCTATGATCGCGAGGAAAGCCCCGCTGCAAAGGACGCCATCGCCCAGATCCTGACCA
ATTCGCGGATGTCCGCCGAAGGCCATCGCCCGATCTGTCAGGACACTGGTATCGGCATGGTTTTCATCAAGGTCGGCATG
CAGGTTACCTGGCCGGATGCCACCATGAGCATCCAGCAGATGATCGACGAAGGCGTGCGTCGCGCCTACGGCAACCCGGA
CAATCCGCTGCGCGCCTCGGTGCTGGCCGACCCGGCCGGTGCCCGCAAGAACACCAAGGACAACACCCCGGCCGTCGTGC
ATTTCGAGATCGTCCCCGGCCACCATGTTGAAGTCATCTGCGCCGCCAAGGGCGGCGGCTCGGAAGCCAAGTCCAAATTC
GCCATGCTCAACCCTTCCGACGACCTGGTCGACTGGGTGCTCAAGAAGATCCCGGAAATGGGCGCCGGTTGGTGCCCGCC
CGGCATCATCGGTATCGGGATCGGCGGCACGCCGGAAAAGGCCATGCTGCTGGCCAAGGAATCGATCATGGCGCCGGTCG
ACATCCATGAACTGAAGGCCAAGGCGGCCACCGGCACCAAGCTGACCCGTCCGGAAGAATTGCGCCTCGAACTGATGGAA
AAGATCAACGCGCTGGGCGTTGGCGCCCAAGGCCTCGGCGGCCTGACCACCGTGCTCGACGTCAAGGTGCTGGATTACCC
CTGCCACGCTGCCAACCTGCCGGTCGCCCTGGTCCCGAACTGTGCTGCAACCCGCCACTGCCATTTCCACCTCGACGGCT
CCGGCCCGGCCAAGCTGGAAGTGCCGAAGCTCGAAGACTGGCCGGCCGTCACGTGGACGCCGGATCTCAAGTCAGCCACG
CAGGTCAACCTGAACACCCTGACCAAGGACGAAGTCGCTTCCTGGAAGCCGGGCCAGAAACTGCTGCTCAACGGCAAGAT
GCTGACTGGCCGCGATGCCGCCCACAAGCGCATTCAGGACATGCTGGCCAAGGGCGAGAAGCTGCCGGTAGATTTCACCA
ACCGCGTCATCTACTACGTCGGCCCGGTCGATCCGGTGCGTGACGAAGTCGTCGGCCCGGCCGGCCCGACCACCGGCACC
CGCATGGACAAGTTCACCCGCATGATGCTGGAACAGACCGGCCTGATCTCGATGATCGGCAAGTCCGAACGCGGTCCGGT
CGCCATCGAGGCGATCAAGGACAACAAGTCGGCCTACCTGATGGCTGTCGGCGGCGCTGCCTACCTGGTTGCCAAGGCGA
TCAAGTCGGCCAAGGTCGTCGGCTTTGCCGATCTGGGCATGGAAGCCATCTACGAGTTCGATGTCGAGAACATGCCGGTC
ACAGTGGCCGTCGATTCATCAGGCATCAGCGTGCACGAAACCGGCCCGAAGGAATGGCAGGTCAAGATCGGCAAGATTCC
GCTCAAGGCCTGA

Upstream 100 bases:

>100_bases
GTATTCCTTTTCTCCGCCCTGGCCATGGCGCTTGTCGACAAGACCATGACCCAGACCCTCGCCCTTCAACACCAGAAATC
CCACTGACCCGGAGTTGTGC

Downstream 100 bases:

>100_bases
TTCCCACCTTGCCCCAAACAAAAGCCCGGCTTCGGCCGGGTTTCTTTTTGGGGAGCGCCGCTGGATCGCCTCAGGCCAAC
CAAGAGCGGATGGCCAATAT

Product: fumarase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MTAIKQEDLIQSVADAFQYISYYHPLDYIKALGEAYDREESPAAKDAIAQILTNSRMSAEGHRPICQDTGIGMVFIKVGM
QVTWPDATMSIQQMIDEGVRRAYGNPDNPLRASVLADPAGARKNTKDNTPAVVHFEIVPGHHVEVICAAKGGGSEAKSKF
AMLNPSDDLVDWVLKKIPEMGAGWCPPGIIGIGIGGTPEKAMLLAKESIMAPVDIHELKAKAATGTKLTRPEELRLELME
KINALGVGAQGLGGLTTVLDVKVLDYPCHAANLPVALVPNCAATRHCHFHLDGSGPAKLEVPKLEDWPAVTWTPDLKSAT
QVNLNTLTKDEVASWKPGQKLLLNGKMLTGRDAAHKRIQDMLAKGEKLPVDFTNRVIYYVGPVDPVRDEVVGPAGPTTGT
RMDKFTRMMLEQTGLISMIGKSERGPVAIEAIKDNKSAYLMAVGGAAYLVAKAIKSAKVVGFADLGMEAIYEFDVENMPV
TVAVDSSGISVHETGPKEWQVKIGKIPLKA

Sequences:

>Translated_510_residues
MTAIKQEDLIQSVADAFQYISYYHPLDYIKALGEAYDREESPAAKDAIAQILTNSRMSAEGHRPICQDTGIGMVFIKVGM
QVTWPDATMSIQQMIDEGVRRAYGNPDNPLRASVLADPAGARKNTKDNTPAVVHFEIVPGHHVEVICAAKGGGSEAKSKF
AMLNPSDDLVDWVLKKIPEMGAGWCPPGIIGIGIGGTPEKAMLLAKESIMAPVDIHELKAKAATGTKLTRPEELRLELME
KINALGVGAQGLGGLTTVLDVKVLDYPCHAANLPVALVPNCAATRHCHFHLDGSGPAKLEVPKLEDWPAVTWTPDLKSAT
QVNLNTLTKDEVASWKPGQKLLLNGKMLTGRDAAHKRIQDMLAKGEKLPVDFTNRVIYYVGPVDPVRDEVVGPAGPTTGT
RMDKFTRMMLEQTGLISMIGKSERGPVAIEAIKDNKSAYLMAVGGAAYLVAKAIKSAKVVGFADLGMEAIYEFDVENMPV
TVAVDSSGISVHETGPKEWQVKIGKIPLKA
>Mature_509_residues
TAIKQEDLIQSVADAFQYISYYHPLDYIKALGEAYDREESPAAKDAIAQILTNSRMSAEGHRPICQDTGIGMVFIKVGMQ
VTWPDATMSIQQMIDEGVRRAYGNPDNPLRASVLADPAGARKNTKDNTPAVVHFEIVPGHHVEVICAAKGGGSEAKSKFA
MLNPSDDLVDWVLKKIPEMGAGWCPPGIIGIGIGGTPEKAMLLAKESIMAPVDIHELKAKAATGTKLTRPEELRLELMEK
INALGVGAQGLGGLTTVLDVKVLDYPCHAANLPVALVPNCAATRHCHFHLDGSGPAKLEVPKLEDWPAVTWTPDLKSATQ
VNLNTLTKDEVASWKPGQKLLLNGKMLTGRDAAHKRIQDMLAKGEKLPVDFTNRVIYYVGPVDPVRDEVVGPAGPTTGTR
MDKFTRMMLEQTGLISMIGKSERGPVAIEAIKDNKSAYLMAVGGAAYLVAKAIKSAKVVGFADLGMEAIYEFDVENMPVT
VAVDSSGISVHETGPKEWQVKIGKIPLKA

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=469, Percent_Identity=32.409381663113, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1790564, Length=468, Percent_Identity=30.7692307692308, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1789442, Length=286, Percent_Identity=29.7202797202797, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1789443, Length=166, Percent_Identity=29.5180722891566, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54930; Mature: 54799

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIKQEDLIQSVADAFQYISYYHPLDYIKALGEAYDREESPAAKDAIAQILTNSRMSAE
CCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCC
GHRPICQDTGIGMVFIKVGMQVTWPDATMSIQQMIDEGVRRAYGNPDNPLRASVLADPAG
CCCCCCCCCCCCEEEEEECCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
ARKNTKDNTPAVVHFEIVPGHHVEVICAAKGGGSEAKSKFAMLNPSDDLVDWVLKKIPEM
CCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCHHHCEEEEECCCHHHHHHHHHHHHHC
GAGWCPPGIIGIGIGGTPEKAMLLAKESIMAPVDIHELKAKAATGTKLTRPEELRLELME
CCCCCCCCEEEECCCCCCHHHHHEEHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHH
KINALGVGAQGLGGLTTVLDVKVLDYPCHAANLPVALVPNCAATRHCHFHLDGSGPAKLE
HHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCCEEECCCCCCCCEEEEEECCCCCCEEE
VPKLEDWPAVTWTPDLKSATQVNLNTLTKDEVASWKPGQKLLLNGKMLTGRDAAHKRIQD
CCCCCCCCCEEECCCCCCCCEEECHHCCHHHHCCCCCCCEEEEECCEECCCHHHHHHHHH
MLAKGEKLPVDFTNRVIYYVGPVDPVRDEVVGPAGPTTGTRMDKFTRMMLEQTGLISMIG
HHHCCCCCCEEECCCEEEEECCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHEECC
KSERGPVAIEAIKDNKSAYLMAVGGAAYLVAKAIKSAKVVGFADLGMEAIYEFDVENMPV
CCCCCCEEEEEEECCCCEEEEEECCHHHHHHHHHHHCEEEEEHHHCHHHHHEECCCCCCE
TVAVDSSGISVHETGPKEWQVKIGKIPLKA
EEEECCCCCEEECCCCCCEEEEEECCCCCC
>Mature Secondary Structure 
TAIKQEDLIQSVADAFQYISYYHPLDYIKALGEAYDREESPAAKDAIAQILTNSRMSAE
CCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCC
GHRPICQDTGIGMVFIKVGMQVTWPDATMSIQQMIDEGVRRAYGNPDNPLRASVLADPAG
CCCCCCCCCCCCEEEEEECCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
ARKNTKDNTPAVVHFEIVPGHHVEVICAAKGGGSEAKSKFAMLNPSDDLVDWVLKKIPEM
CCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCHHHCEEEEECCCHHHHHHHHHHHHHC
GAGWCPPGIIGIGIGGTPEKAMLLAKESIMAPVDIHELKAKAATGTKLTRPEELRLELME
CCCCCCCCEEEECCCCCCHHHHHEEHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHH
KINALGVGAQGLGGLTTVLDVKVLDYPCHAANLPVALVPNCAATRHCHFHLDGSGPAKLE
HHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCCEEECCCCCCCCEEEEEECCCCCCEEE
VPKLEDWPAVTWTPDLKSATQVNLNTLTKDEVASWKPGQKLLLNGKMLTGRDAAHKRIQD
CCCCCCCCCEEECCCCCCCCEEECHHCCHHHHCCCCCCCEEEEECCEECCCHHHHHHHHH
MLAKGEKLPVDFTNRVIYYVGPVDPVRDEVVGPAGPTTGTRMDKFTRMMLEQTGLISMIG
HHHCCCCCCEEECCCEEEEECCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHEECC
KSERGPVAIEAIKDNKSAYLMAVGGAAYLVAKAIKSAKVVGFADLGMEAIYEFDVENMPV
CCCCCCEEEEEEECCCCEEEEEECCHHHHHHHHHHHCEEEEEHHHCHHHHHEECCCCCCE
TVAVDSSGISVHETGPKEWQVKIGKIPLKA
EEEECCCCCEEECCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]