Click here to switch to the map view.

The map label for this gene is rnhA

Identifier: 71907225

GI number: 71907225

Start: 1709113

End: 1709559

Strand: Reverse

Name: rnhA

Synonym: Daro_1593

Alternate gene names: 71907225

Gene position: 1709559-1709113 (Counterclockwise)

Preceding gene: 71907226

Following gene: 71907224

Centisome position: 37.98

GC content: 56.82

Gene sequence:

>447_bases
ATGACCGCTGAGGAAACTGTTGAAATATTCACCGATGGCGCCTGCAAGGGTAACCCTGGCCCCGGTGGCTGGGGGGCCAT
CCTGCGCCTCGGACCACACGAGAAGGAACTGTGGGGAGGCGAGAAGGAAACGACCAACAACCGTATGGAACTGACCGCTG
CCATCCGCGCCATCGAAGCGCTGAAACGCCCCATCGGAGGCAAGATCTACACCGACTCGCAATACGTCATGAAGGGCATC
AACGAGTGGATTCATGGCTGGAAGAAGAATGGCTGGAAGACTTCGGACAAGAAGCCGGTGAAAAACGCCGATCTCTGGCA
ACTGCTCGATGCCCAAGTCAAGTTGCACAAGCTGGAATGGATCTGGGTCAGGGGGCATTCCGGGCATCCGGAAAACGAAC
GCGCCGATGCATTGGCCAACCGTGGCATCGAAGAATTGAAGGGTTAA

Upstream 100 bases:

>100_bases
GCCATCAAGCGCACGCACAGCATGCGCCTGATCACCCCAAAATGGAAAAAACAGGCAGTACGGGCCAAGGCACTGCGCCC
GATTGCTCAAAAGGAACGTC

Downstream 100 bases:

>100_bases
GGAAGCACCATGCGACAGATCGTTCTCGACACTGAAACCACCGGCCTAGACCCCCGCTCAGGCCACCGTATCATCGAAGT
GGCGTGCATCGAGATGGTGA

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MTAEETVEIFTDGACKGNPGPGGWGAILRLGPHEKELWGGEKETTNNRMELTAAIRAIEALKRPIGGKIYTDSQYVMKGI
NEWIHGWKKNGWKTSDKKPVKNADLWQLLDAQVKLHKLEWIWVRGHSGHPENERADALANRGIEELKG

Sequences:

>Translated_148_residues
MTAEETVEIFTDGACKGNPGPGGWGAILRLGPHEKELWGGEKETTNNRMELTAAIRAIEALKRPIGGKIYTDSQYVMKGI
NEWIHGWKKNGWKTSDKKPVKNADLWQLLDAQVKLHKLEWIWVRGHSGHPENERADALANRGIEELKG
>Mature_147_residues
TAEETVEIFTDGACKGNPGPGGWGAILRLGPHEKELWGGEKETTNNRMELTAAIRAIEALKRPIGGKIYTDSQYVMKGIN
EWIHGWKKNGWKTSDKKPVKNADLWQLLDAQVKLHKLEWIWVRGHSGHPENERADALANRGIEELKG

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=152, Percent_Identity=38.8157894736842, Blast_Score=84, Evalue=5e-17,
Organism=Escherichia coli, GI1786408, Length=133, Percent_Identity=65.4135338345865, Blast_Score=181, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI115532542, Length=141, Percent_Identity=39.0070921985816, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45552509, Length=149, Percent_Identity=36.9127516778523, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI17137476, Length=149, Percent_Identity=36.9127516778523, Blast_Score=77, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_DECAR (Q47FN9)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_284812.1
- HSSP:   P0A7Y4
- ProteinModelPortal:   Q47FN9
- SMR:   Q47FN9
- STRING:   Q47FN9
- GeneID:   3568737
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_1593
- NMPDR:   fig|159087.4.peg.1609
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- BioCyc:   DARO159087:DARO_1593-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 16622; Mature: 16491

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAEETVEIFTDGACKGNPGPGGWGAILRLGPHEKELWGGEKETTNNRMELTAAIRAIEA
CCCCHHHHHHCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHHH
LKRPIGGKIYTDSQYVMKGINEWIHGWKKNGWKTSDKKPVKNADLWQLLDAQVKLHKLEW
HHCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEEE
IWVRGHSGHPENERADALANRGIEELKG
EEEECCCCCCCCHHHHHHHHCCHHHHCC
>Mature Secondary Structure 
TAEETVEIFTDGACKGNPGPGGWGAILRLGPHEKELWGGEKETTNNRMELTAAIRAIEA
CCCHHHHHHCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHHH
LKRPIGGKIYTDSQYVMKGINEWIHGWKKNGWKTSDKKPVKNADLWQLLDAQVKLHKLEW
HHCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEEE
IWVRGHSGHPENERADALANRGIEELKG
EEEECCCCCCCCHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA