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The map label for this gene is nirT [H]

Identifier: 71907214

GI number: 71907214

Start: 1700136

End: 1700723

Strand: Reverse

Name: nirT [H]

Synonym: Daro_1582

Alternate gene names: 71907214

Gene position: 1700723-1700136 (Counterclockwise)

Preceding gene: 71907217

Following gene: 71907213

Centisome position: 37.78

GC content: 54.08

Gene sequence:

>588_bases
ATGATTTCAATCTGGCAACGCTTCCGTGCCCAAAAGCCTTGGGCAAAAATTCTCGAAGTCGAGGCGATCGTCCTGATGTT
GATTGGTGCGCTATTGATGGCCGGCGGCAAGCTGTCGCTCGACCATACGAATACGATCGAGTTTTGCACCTCCTGCCACG
AGATGAAGAACAACAACTACGAGGAATACAAGCACACCATCCACGCCATGAACCGCACCGGCGTCAAGGCAACCTGCTCC
GACTGCCACGTGCCGCACGAATTCGTCGACACGATGATCCGCAAGATCAAGGCCTCCAATGATGTCTTTCAGCACCTGAT
GGGCACCATCGACACCAAGGAGAAGTTCGAAGCACATCGCCTTGAACTCGCCAAGCGAGTCTGGCTACGCATGAAGGAAA
GCGACTCCCGGGAATGCCGGAACTGTCATGACACCAAGGCCATGGACCCGGAAAAACAGGGGAAAACGGCCCAGAAGCAA
CATCAAAAACTGGCTTCTGGTGAGCGGACCTGTATCGATTGTCACTATGGCATCGCCCATAAGGAACCCGAAGGGGGAGT
TGAGCCTCGTGATGTCGTGGGAAAATAG

Upstream 100 bases:

>100_bases
CGAGGCAAGCTTGCGAATGCCTGACGAATAGGAAAACTCGTCAGGCATTCGCAAGCTAGGTGTTGCGATGATGCGGGATC
AACTAAATTAGGAAACCGCA

Downstream 100 bases:

>100_bases
CGAAGCAACAGATTCACGACAACACTGCAGCTAAAACATATGCGCATCCTGATCGTTGAAGATGACAATCTTGTCGCCCA
GGGCCTGAAGCAGGGACTTC

Product: NapC/NirT cytochrome c, N-terminal

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MISIWQRFRAQKPWAKILEVEAIVLMLIGALLMAGGKLSLDHTNTIEFCTSCHEMKNNNYEEYKHTIHAMNRTGVKATCS
DCHVPHEFVDTMIRKIKASNDVFQHLMGTIDTKEKFEAHRLELAKRVWLRMKESDSRECRNCHDTKAMDPEKQGKTAQKQ
HQKLASGERTCIDCHYGIAHKEPEGGVEPRDVVGK

Sequences:

>Translated_195_residues
MISIWQRFRAQKPWAKILEVEAIVLMLIGALLMAGGKLSLDHTNTIEFCTSCHEMKNNNYEEYKHTIHAMNRTGVKATCS
DCHVPHEFVDTMIRKIKASNDVFQHLMGTIDTKEKFEAHRLELAKRVWLRMKESDSRECRNCHDTKAMDPEKQGKTAQKQ
HQKLASGERTCIDCHYGIAHKEPEGGVEPRDVVGK
>Mature_195_residues
MISIWQRFRAQKPWAKILEVEAIVLMLIGALLMAGGKLSLDHTNTIEFCTSCHEMKNNNYEEYKHTIHAMNRTGVKATCS
DCHVPHEFVDTMIRKIKASNDVFQHLMGTIDTKEKFEAHRLELAKRVWLRMKESDSRECRNCHDTKAMDPEKQGKTAQKQ
HQKLASGERTCIDCHYGIAHKEPEGGVEPRDVVGK

Specific function: Mediates Electron Flow From Quinones To The Napab Complex. [C]

COG id: COG3005

COG function: function code C; Nitrate/TMAO reductases, membrane-bound tetraheme cytochrome c subunit

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788530, Length=182, Percent_Identity=46.1538461538462, Blast_Score=184, Evalue=4e-48,
Organism=Escherichia coli, GI1787230, Length=185, Percent_Identity=43.7837837837838, Blast_Score=157, Evalue=4e-40,
Organism=Escherichia coli, GI1788181, Length=146, Percent_Identity=46.5753424657534, Blast_Score=146, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005126
- InterPro:   IPR011885 [H]

Pfam domain/function: PF03264 Cytochrom_NNT [H]

EC number: NA

Molecular weight: Translated: 22374; Mature: 22374

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
8.7 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
8.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISIWQRFRAQKPWAKILEVEAIVLMLIGALLMAGGKLSLDHTNTIEFCTSCHEMKNNNY
CCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCH
EEYKHTIHAMNRTGVKATCSDCHVPHEFVDTMIRKIKASNDVFQHLMGTIDTKEKFEAHR
HHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHH
LELAKRVWLRMKESDSRECRNCHDTKAMDPEKQGKTAQKQHQKLASGERTCIDCHYGIAH
HHHHHHHHHHHCCCCCHHHHHHCCCCCCCCHHCCCHHHHHHHHHHCCCCEEEEECCCCCC
KEPEGGVEPRDVVGK
CCCCCCCCCHHHCCC
>Mature Secondary Structure
MISIWQRFRAQKPWAKILEVEAIVLMLIGALLMAGGKLSLDHTNTIEFCTSCHEMKNNNY
CCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCH
EEYKHTIHAMNRTGVKATCSDCHVPHEFVDTMIRKIKASNDVFQHLMGTIDTKEKFEAHR
HHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHH
LELAKRVWLRMKESDSRECRNCHDTKAMDPEKQGKTAQKQHQKLASGERTCIDCHYGIAH
HHHHHHHHHHHCCCCCHHHHHHCCCCCCCCHHCCCHHHHHHHHHHCCCCEEEEECCCCCC
KEPEGGVEPRDVVGK
CCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2001732 [H]