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The map label for this gene is nifD [H]

Identifier: 71907046

GI number: 71907046

Start: 1543076

End: 1544548

Strand: Reverse

Name: nifD [H]

Synonym: Daro_1414

Alternate gene names: 71907046

Gene position: 1544548-1543076 (Counterclockwise)

Preceding gene: 71907047

Following gene: 71907045

Centisome position: 34.31

GC content: 58.72

Gene sequence:

>1473_bases
ATGACGACTCTGTCTCGCGAAGAAACCGAAGCCCTCATTGCCGAGGTGCTTGAGGTTTATCCGGAAAAAGCCCAGAAGGA
CCGTGCCAAGCATCTGGCGGTCAATGACCAATCTGTTGAACAATCCAAGAAGTGCATCACTTCCAACCGCAAGTCCCTGC
CGGGCGTCATGACCATTCGTGGTTGTGCCTACGCTGGCTCCAAGGGCGTGGTCTGGGGTCCGATCAAGGACATGATCCAC
ATCTCGCACGGCCCTGTCGGCTGCGGTCAGTATTCCCGCGCCGGTCGCCGTAACTACTACGTCGGCACCACCGGCGTCGA
TACCTTCGGCACGATGAACTTCACCTCCGATTTCCAGGAGAAGGACATTGTGTTCGGCGGCGACAAGAAGCTCGCCAAGC
TGATCGACGAAGTCGAACTGCTCTTCCCGCTGCACAAGGGAATCTCGGTCCAGTCCGAGTGCCCGATCGGTCTGATCGGC
GACGACATCGAGTCCGTCTCCAAGAAGGCTGCCGCCGTCATCGACAAGCCGGTCGTCCCGGTGCGTTGCGAAGGCTTCCG
CGGTGTTTCCCAGTCCCTCGGCCACCACATCGCCAACGACGCGATCCGTGACTGGGTGCTCGACAAGCGCGACGGCGCCG
CCTTCGAATCGACTCCGTACGACGTCGCCATCATCGGTGACTACAACATCGGTGGCGACGCCTGGGCCACCCGCATCCTG
CTCGAAGAGATGGGCCTGCGTGTTGTGGCTCAGTGGTCCGGCGACGGCACCATTGCCGAAATGATGAACACGCCGAAGGT
CAAGCTGAACCTGCTGCACTGCTACCGTTCGATGAACTACATCTCGCGTCACATGGAAGAGAAGTACGGCATTCCTTACC
TGGAATACAACTTCTTCGGCCCGACCAAGATCATCGAATCCCTGCGAAAGATCGCCGCCTTCTTCGACGATTCCGTCAAG
GAAAAGACCGAAGCCGTGATCGCCCGCTACAAGCCGATGATGGACGAGATCATCGCCAAGTACAAACCGCGTCTCGAGGG
CAAGAAGGTCATGCTCTACGTCGGCGGCCTGCGTCCGCGTCACGTTATCGGCGCCTACGAAGATCTCGGCATGGAAGTCA
TCGGCACCGGCTACGAATTCGGCCACAACGACGACTACGACCGCACGATCAAAGAGATGGGCGATGCCACCCTGCTCTAC
GACGACGTCACCGGTTTCGAGCTGGAAGAATTCGTCAAGCGCCTGAAGCCCGACATGGTCGGCTCCGGCATCAAGGAAAA
GTACATCTTCCAGAAGATGGGCATTCCGCTGCGCCAGATGCACTCCTGGGATTACTCGGGCCCGTACCACGGTTACGACG
GTTTCGCGATCTTCGCCCGTGACATGGACATCGCCCTGTCCAACCCGACCTTCAAGAACCTTGTTCCGCCATGGAAGAAG
GTTGCTGCTGAAGAAGTCAAGAAGGCCGCCTGA

Upstream 100 bases:

>100_bases
TGCCGCCTAAGCTGTGCAAGACACGGCGGGGTGCTCAAGCGCCCCGCCACTTTCAACCAGTACGTCGGCCACAGATTGGT
GGCGGCGTCAGGAGGAAAAC

Downstream 100 bases:

>100_bases
CCTAGCTCAGTAAATCCTTAACCTTGAGCCCGCAGCCGCGAATGAGCGGGGCGGGCAAGGAGATAGCAATGCAAACCGCA
GACAACATCAAGCCCTGTTA

Product: nitrogenase molybdenum-iron protein alpha chain:nitrogenase component I, alpha chain

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MTTLSREETEALIAEVLEVYPEKAQKDRAKHLAVNDQSVEQSKKCITSNRKSLPGVMTIRGCAYAGSKGVVWGPIKDMIH
ISHGPVGCGQYSRAGRRNYYVGTTGVDTFGTMNFTSDFQEKDIVFGGDKKLAKLIDEVELLFPLHKGISVQSECPIGLIG
DDIESVSKKAAAVIDKPVVPVRCEGFRGVSQSLGHHIANDAIRDWVLDKRDGAAFESTPYDVAIIGDYNIGGDAWATRIL
LEEMGLRVVAQWSGDGTIAEMMNTPKVKLNLLHCYRSMNYISRHMEEKYGIPYLEYNFFGPTKIIESLRKIAAFFDDSVK
EKTEAVIARYKPMMDEIIAKYKPRLEGKKVMLYVGGLRPRHVIGAYEDLGMEVIGTGYEFGHNDDYDRTIKEMGDATLLY
DDVTGFELEEFVKRLKPDMVGSGIKEKYIFQKMGIPLRQMHSWDYSGPYHGYDGFAIFARDMDIALSNPTFKNLVPPWKK
VAAEEVKKAA

Sequences:

>Translated_490_residues
MTTLSREETEALIAEVLEVYPEKAQKDRAKHLAVNDQSVEQSKKCITSNRKSLPGVMTIRGCAYAGSKGVVWGPIKDMIH
ISHGPVGCGQYSRAGRRNYYVGTTGVDTFGTMNFTSDFQEKDIVFGGDKKLAKLIDEVELLFPLHKGISVQSECPIGLIG
DDIESVSKKAAAVIDKPVVPVRCEGFRGVSQSLGHHIANDAIRDWVLDKRDGAAFESTPYDVAIIGDYNIGGDAWATRIL
LEEMGLRVVAQWSGDGTIAEMMNTPKVKLNLLHCYRSMNYISRHMEEKYGIPYLEYNFFGPTKIIESLRKIAAFFDDSVK
EKTEAVIARYKPMMDEIIAKYKPRLEGKKVMLYVGGLRPRHVIGAYEDLGMEVIGTGYEFGHNDDYDRTIKEMGDATLLY
DDVTGFELEEFVKRLKPDMVGSGIKEKYIFQKMGIPLRQMHSWDYSGPYHGYDGFAIFARDMDIALSNPTFKNLVPPWKK
VAAEEVKKAA
>Mature_489_residues
TTLSREETEALIAEVLEVYPEKAQKDRAKHLAVNDQSVEQSKKCITSNRKSLPGVMTIRGCAYAGSKGVVWGPIKDMIHI
SHGPVGCGQYSRAGRRNYYVGTTGVDTFGTMNFTSDFQEKDIVFGGDKKLAKLIDEVELLFPLHKGISVQSECPIGLIGD
DIESVSKKAAAVIDKPVVPVRCEGFRGVSQSLGHHIANDAIRDWVLDKRDGAAFESTPYDVAIIGDYNIGGDAWATRILL
EEMGLRVVAQWSGDGTIAEMMNTPKVKLNLLHCYRSMNYISRHMEEKYGIPYLEYNFFGPTKIIESLRKIAAFFDDSVKE
KTEAVIARYKPMMDEIIAKYKPRLEGKKVMLYVGGLRPRHVIGAYEDLGMEVIGTGYEFGHNDDYDRTIKEMGDATLLYD
DVTGFELEEFVKRLKPDMVGSGIKEKYIFQKMGIPLRQMHSWDYSGPYHGYDGFAIFARDMDIALSNPTFKNLVPPWKKV
AAEEVKKAA

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR010143
- InterPro:   IPR000318
- InterPro:   IPR005972 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 54895; Mature: 54763

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLSREETEALIAEVLEVYPEKAQKDRAKHLAVNDQSVEQSKKCITSNRKSLPGVMTIR
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHEECCCHHHHHHHHHHHCCCCCCCCEEEEE
GCAYAGSKGVVWGPIKDMIHISHGPVGCGQYSRAGRRNYYVGTTGVDTFGTMNFTSDFQE
EEEECCCCCCEECCHHHHEEECCCCCCCCHHHHCCCCCEEEECCCCCCCCCCCCCCCCCC
KDIVFGGDKKLAKLIDEVELLFPLHKGISVQSECPIGLIGDDIESVSKKAAAVIDKPVVP
CCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCC
VRCEGFRGVSQSLGHHIANDAIRDWVLDKRDGAAFESTPYDVAIIGDYNIGGDAWATRIL
EEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
LEEMGLRVVAQWSGDGTIAEMMNTPKVKLNLLHCYRSMNYISRHMEEKYGIPYLEYNFFG
HHHCCCEEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
PTKIIESLRKIAAFFDDSVKEKTEAVIARYKPMMDEIIAKYKPRLEGKKVMLYVGGLRPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCH
HVIGAYEDLGMEVIGTGYEFGHNDDYDRTIKEMGDATLLYDDVTGFELEEFVKRLKPDMV
HHHCCHHHCCCEEEECCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCHHHH
GSGIKEKYIFQKMGIPLRQMHSWDYSGPYHGYDGFAIFARDMDIALSNPTFKNLVPPWKK
CCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCEEEEEECCEEEECCCCHHHCCCHHHH
VAAEEVKKAA
HHHHHHHHCC
>Mature Secondary Structure 
TTLSREETEALIAEVLEVYPEKAQKDRAKHLAVNDQSVEQSKKCITSNRKSLPGVMTIR
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHEECCCHHHHHHHHHHHCCCCCCCCEEEEE
GCAYAGSKGVVWGPIKDMIHISHGPVGCGQYSRAGRRNYYVGTTGVDTFGTMNFTSDFQE
EEEECCCCCCEECCHHHHEEECCCCCCCCHHHHCCCCCEEEECCCCCCCCCCCCCCCCCC
KDIVFGGDKKLAKLIDEVELLFPLHKGISVQSECPIGLIGDDIESVSKKAAAVIDKPVVP
CCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCC
VRCEGFRGVSQSLGHHIANDAIRDWVLDKRDGAAFESTPYDVAIIGDYNIGGDAWATRIL
EEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
LEEMGLRVVAQWSGDGTIAEMMNTPKVKLNLLHCYRSMNYISRHMEEKYGIPYLEYNFFG
HHHCCCEEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
PTKIIESLRKIAAFFDDSVKEKTEAVIARYKPMMDEIIAKYKPRLEGKKVMLYVGGLRPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCH
HVIGAYEDLGMEVIGTGYEFGHNDDYDRTIKEMGDATLLYDDVTGFELEEFVKRLKPDMV
HHHCCHHHCCCEEEECCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCHHHH
GSGIKEKYIFQKMGIPLRQMHSWDYSGPYHGYDGFAIFARDMDIALSNPTFKNLVPPWKK
CCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCEEEEEECCEEEECCCCHHHCCCHHHH
VAAEEVKKAA
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644218; 3344210; 3863780; 649581; 9163420; 9063865; 11170380; 11951047; 12215645 [H]