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The map label for this gene is nifH

Identifier: 71907047

GI number: 71907047

Start: 1544639

End: 1545529

Strand: Reverse

Name: nifH

Synonym: Daro_1415

Alternate gene names: 71907047

Gene position: 1545529-1544639 (Counterclockwise)

Preceding gene: 71907049

Following gene: 71907046

Centisome position: 34.34

GC content: 58.36

Gene sequence:

>891_bases
ATGGCAAAACTCCGTCAATGCGCCATCTACGGCAAAGGCGGTATCGGCAAGTCCACCACCACCCAGAATCTGGTGGCGGC
ACTCGCTGAATCCGGCAAGAAAGTTATGATCGTTGGCTGCGACCCGAAAGCCGACTCAACCCGCCTGATCCTGCATAGCA
AGGCTCAGACCACCGTGATGCACCTGGCTGCTGAAGCCGGCTCGGTGGAAGACCTCGAACTGGAAGACGTGCTCTCCGTC
GGCTTCGGCGGCATCAAATGCGTTGAATCCGGTGGCCCGGAACCCGGTGTTGGTTGTGCCGGTCGCGGCGTCATCACCGC
CATCAACTTCCTCGAAGAAGAAGGCGCTTATGACGAAGACCTCGACTTCGTGTTCTACGACGTGCTGGGTGACGTGGTCT
GCGGTGGCTTCGCCATGCCGATCCGTGAAAACAAGGCGCAGGAAATCTACATCGTCTGTTCCGGCGAAATGATGGCCATG
TACGCCGCCAACAACATTGCCAAGGGCATCGTGAAGTATGCGAACTCCGGCGGTGTCCGTCTGGCCGGCCTGATCTGCAA
CAGCCGCAACACTGACCGCGAAGACGAATTGATCATGGCCCTGGCTGGTCGCCTCGGCACCACGATGATCCACTTCGTGC
CGCGTGACAACGCTGTTCAGCACGCTGAAATCCGCCGCATGACCATGGTCGAATACGATCCGAAGCACAAGCAGGCTGAC
GAATATCGCCAGCTCGCCAACAAGATCGTCAATAACACCAACTTCGTCATCCCGACCCCGATCGAGATGGAAGAGCTGGA
AGAACTGCTGATGGAATTCGGCATCATGGAAGCGGAAGACGAGTCCATCGTCGGCCAGACCGCTGCCGAACTGGCTGCTG
CTGCCGCCTAA

Upstream 100 bases:

>100_bases
TACGGCCTGCCAGTCGACCTGGCACGCTACATGCAGAAGGAGAGACGCGACTCTCAAAGATTCCTTCGCAAATCGACTGA
CAGACTAAGGAGATTACACC

Downstream 100 bases:

>100_bases
GCTGTGCAAGACACGGCGGGGTGCTCAAGCGCCCCGCCACTTTCAACCAGTACGTCGGCCACAGATTGGTGGCGGCGTCA
GGAGGAAAACATGACGACTC

Product: nitrogenase iron protein subunit NifH

Products: NA

Alternate protein names: Nitrogenase Fe protein; Nitrogenase component II; Nitrogenase reductase

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MAKLRQCAIYGKGGIGKSTTTQNLVAALAESGKKVMIVGCDPKADSTRLILHSKAQTTVMHLAAEAGSVEDLELEDVLSV
GFGGIKCVESGGPEPGVGCAGRGVITAINFLEEEGAYDEDLDFVFYDVLGDVVCGGFAMPIRENKAQEIYIVCSGEMMAM
YAANNIAKGIVKYANSGGVRLAGLICNSRNTDREDELIMALAGRLGTTMIHFVPRDNAVQHAEIRRMTMVEYDPKHKQAD
EYRQLANKIVNNTNFVIPTPIEMEELEELLMEFGIMEAEDESIVGQTAAELAAAAA

Sequences:

>Translated_296_residues
MAKLRQCAIYGKGGIGKSTTTQNLVAALAESGKKVMIVGCDPKADSTRLILHSKAQTTVMHLAAEAGSVEDLELEDVLSV
GFGGIKCVESGGPEPGVGCAGRGVITAINFLEEEGAYDEDLDFVFYDVLGDVVCGGFAMPIRENKAQEIYIVCSGEMMAM
YAANNIAKGIVKYANSGGVRLAGLICNSRNTDREDELIMALAGRLGTTMIHFVPRDNAVQHAEIRRMTMVEYDPKHKQAD
EYRQLANKIVNNTNFVIPTPIEMEELEELLMEFGIMEAEDESIVGQTAAELAAAAA
>Mature_295_residues
AKLRQCAIYGKGGIGKSTTTQNLVAALAESGKKVMIVGCDPKADSTRLILHSKAQTTVMHLAAEAGSVEDLELEDVLSVG
FGGIKCVESGGPEPGVGCAGRGVITAINFLEEEGAYDEDLDFVFYDVLGDVVCGGFAMPIRENKAQEIYIVCSGEMMAMY
AANNIAKGIVKYANSGGVRLAGLICNSRNTDREDELIMALAGRLGTTMIHFVPRDNAVQHAEIRRMTMVEYDPKHKQADE
YRQLANKIVNNTNFVIPTPIEMEELEELLMEFGIMEAEDESIVGQTAAELAAAAA

Specific function: The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components:the iron protein and the molybdenum-iron protein

COG id: COG1348

COG function: function code P; Nitrogenase subunit NifH (ATPase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NifH/BchL/ChlL family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NIFH_DECAR (Q47G67)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_284634.1
- HSSP:   P00459
- ProteinModelPortal:   Q47G67
- SMR:   Q47G67
- STRING:   Q47G67
- GeneID:   3569100
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_1415
- NMPDR:   fig|159087.4.peg.1268
- eggNOG:   COG1348
- HOGENOM:   HBG488165
- OMA:   SDENIRQ
- ProtClustDB:   CLSK2486516
- BioCyc:   DARO159087:DARO_1415-MONOMER
- HAMAP:   MF_00533
- InterPro:   IPR000392
- InterPro:   IPR005977
- PIRSF:   PIRSF000363
- PRINTS:   PR00091
- TIGRFAMs:   TIGR01287

Pfam domain/function: PF00142 Fer4_NifH

EC number: =1.18.6.1

Molecular weight: Translated: 31832; Mature: 31701

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS00746 NIFH_FRXC_1; PS00692 NIFH_FRXC_2; PS51026 NIFH_FRXC_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLRQCAIYGKGGIGKSTTTQNLVAALAESGKKVMIVGCDPKADSTRLILHSKAQTTVM
CCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCHHHHHH
HLAAEAGSVEDLELEDVLSVGFGGIKCVESGGPEPGVGCAGRGVITAINFLEEEGAYDED
HHHHCCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
LDFVFYDVLGDVVCGGFAMPIRENKAQEIYIVCSGEMMAMYAANNIAKGIVKYANSGGVR
HHHHHHHHHHHHHHCCEECCCCCCCCCEEEEEECCCEEEEHHHHHHHHHHHHHCCCCCEE
LAGLICNSRNTDREDELIMALAGRLGTTMIHFVPRDNAVQHAEIRRMTMVEYDPKHKQAD
EEEEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHEEEEECCCCCCHH
EYRQLANKIVNNTNFVIPTPIEMEELEELLMEFGIMEAEDESIVGQTAAELAAAAA
HHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKLRQCAIYGKGGIGKSTTTQNLVAALAESGKKVMIVGCDPKADSTRLILHSKAQTTVM
CCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCHHHHHH
HLAAEAGSVEDLELEDVLSVGFGGIKCVESGGPEPGVGCAGRGVITAINFLEEEGAYDED
HHHHCCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
LDFVFYDVLGDVVCGGFAMPIRENKAQEIYIVCSGEMMAMYAANNIAKGIVKYANSGGVR
HHHHHHHHHHHHHHCCEECCCCCCCCCEEEEEECCCEEEEHHHHHHHHHHHHHCCCCCEE
LAGLICNSRNTDREDELIMALAGRLGTTMIHFVPRDNAVQHAEIRRMTMVEYDPKHKQAD
EEEEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHEEEEECCCCCCHH
EYRQLANKIVNNTNFVIPTPIEMEELEELLMEFGIMEAEDESIVGQTAAELAAAAA
HHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA