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The map label for this gene is gltX

Identifier: 71906954

GI number: 71906954

Start: 1438601

End: 1439995

Strand: Reverse

Name: gltX

Synonym: Daro_1321

Alternate gene names: 71906954

Gene position: 1439995-1438601 (Counterclockwise)

Preceding gene: 71906955

Following gene: 71906953

Centisome position: 31.99

GC content: 58.92

Gene sequence:

>1395_bases
ATGAAGCCAGTCCGCACCCGTTTCGCCCCCAGTCCCACCGGTTATCTGCACATCGGCGGCGCCCGTACTGCCCTGTTCTC
CTGGGCTTATGCCCGACGCCACGGTGGCGATTTCATCCTGCGCATTGAAGATACCGACGTTGCTCGCTCGACGCCGGAAG
CCGTACAGGCCATTCTCGACGGCATGCAATGGCTGGGTCTGGAACATGACGAAGGTCCGTTCTACCAGATGCAGCGCATG
GACCGCTACAAGGAAGTCATCCAGCAGATGCTTGCCAACGGCAGCGCCTATTACTGCTACACGACCCGCGAGGAACTCGA
TGCCTTGCGTGCCGAGCAGGAAGCAAAGAAGGAAAAGCCGCGCTACGACGGCCGCTGGCGCCCCGAGGCTGGAAAAGCCC
TGCCCGTGCCACCAACTGATGTTCCGCCGGTCATTCGCTTCAAGAATCCGCAGGGCGGCGTTGTGGCTTGGGACGATCAG
GTCAAGGGCCGCATCGAGTTTGCCAATACTGAACTGGACGACCTGATCATCGCCCGCGCCGATGGCACCCCAACCTACAA
TTTCTGCGTCTGCGTCGACGACTGGGATATGGGCATCACCCACGTCATCCGTGGTGACGACCACGTCAATAATACCCCGC
GTCAGATCAACATCCTCAAGGCGCTGGGCGCAGAAGTGCCAACCTACGCCCACCTGTCGATGATTCTTGGCGACGATGGC
GCCAAGCTATCAAAGCGCCATGGCGCCGTATCTGTCATGCAGTATGACGAGGAAGGTTTTCTGACCGAGGCCGTGATCAA
CTATCTTGCCCGTCTTGGCTGGTCGCATGGCGATGATGAAGTTTTTTCACGCCAACAGTTCGTCGAATGGTTCGACCTTG
ACCACATCACCGCCTCCGCCGCCCAGTTCAACACCGAAAAACTGCTGTGGCTGAACCAGCATTACATGAAACAACTACCG
CCGGCCGAACTGGCCGCCAAGGTTCAAGCCCGACTGACAGCGCGTGGCGTGGATACGGCCAACGGCCCGGATCTTGAAAA
AGCAGTTGTTTTGTATGTCGACCGCAGCAATACGCTGAATGTCCTGGCCGATGCCGTCGAGGTGTTCTACGCCCATGTGA
CGCCCAACCCGGAACTTTTGGCCCAACACCTTGCCGATGACGCCCGCCCCGCCCTGGCCGAGTTTGCAGCCGGCATCGCC
ACAGTCACCTGGGAAGCACCGGCTATCAACGCCTTGATCAAGGAAACCGTGACCAAACATGGCCTGAAGATGCCCAAACT
AGCGATGCCGCTACGGGTGATTCTGACTGGACAGGCACAAACCCCGGCTGTCGATGCAGTGATTGCATTGATCGGGCGCG
ACAAGGTGGCAGCGACCCTGGCAGCTTATCTCTAA

Upstream 100 bases:

>100_bases
CGCCCTCACAAGAGGCGCGCATTTAACCACAAGTCGAGCAGCGGGTCAAACGCGGGTCGGCTAAAATACCCGCTTTGCCA
ACGTATAGACTCCGTACTCC

Downstream 100 bases:

>100_bases
AAAATAGCGAAAGGCCCTTTACAAGGACCAGGACGGTCCCTATAATGCGGCCTTCTTTCGCGGCGGGGGTATAGCTCAGC
TGGGAGAGCGCTTGCATGGC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MKPVRTRFAPSPTGYLHIGGARTALFSWAYARRHGGDFILRIEDTDVARSTPEAVQAILDGMQWLGLEHDEGPFYQMQRM
DRYKEVIQQMLANGSAYYCYTTREELDALRAEQEAKKEKPRYDGRWRPEAGKALPVPPTDVPPVIRFKNPQGGVVAWDDQ
VKGRIEFANTELDDLIIARADGTPTYNFCVCVDDWDMGITHVIRGDDHVNNTPRQINILKALGAEVPTYAHLSMILGDDG
AKLSKRHGAVSVMQYDEEGFLTEAVINYLARLGWSHGDDEVFSRQQFVEWFDLDHITASAAQFNTEKLLWLNQHYMKQLP
PAELAAKVQARLTARGVDTANGPDLEKAVVLYVDRSNTLNVLADAVEVFYAHVTPNPELLAQHLADDARPALAEFAAGIA
TVTWEAPAINALIKETVTKHGLKMPKLAMPLRVILTGQAQTPAVDAVIALIGRDKVAATLAAYL

Sequences:

>Translated_464_residues
MKPVRTRFAPSPTGYLHIGGARTALFSWAYARRHGGDFILRIEDTDVARSTPEAVQAILDGMQWLGLEHDEGPFYQMQRM
DRYKEVIQQMLANGSAYYCYTTREELDALRAEQEAKKEKPRYDGRWRPEAGKALPVPPTDVPPVIRFKNPQGGVVAWDDQ
VKGRIEFANTELDDLIIARADGTPTYNFCVCVDDWDMGITHVIRGDDHVNNTPRQINILKALGAEVPTYAHLSMILGDDG
AKLSKRHGAVSVMQYDEEGFLTEAVINYLARLGWSHGDDEVFSRQQFVEWFDLDHITASAAQFNTEKLLWLNQHYMKQLP
PAELAAKVQARLTARGVDTANGPDLEKAVVLYVDRSNTLNVLADAVEVFYAHVTPNPELLAQHLADDARPALAEFAAGIA
TVTWEAPAINALIKETVTKHGLKMPKLAMPLRVILTGQAQTPAVDAVIALIGRDKVAATLAAYL
>Mature_464_residues
MKPVRTRFAPSPTGYLHIGGARTALFSWAYARRHGGDFILRIEDTDVARSTPEAVQAILDGMQWLGLEHDEGPFYQMQRM
DRYKEVIQQMLANGSAYYCYTTREELDALRAEQEAKKEKPRYDGRWRPEAGKALPVPPTDVPPVIRFKNPQGGVVAWDDQ
VKGRIEFANTELDDLIIARADGTPTYNFCVCVDDWDMGITHVIRGDDHVNNTPRQINILKALGAEVPTYAHLSMILGDDG
AKLSKRHGAVSVMQYDEEGFLTEAVINYLARLGWSHGDDEVFSRQQFVEWFDLDHITASAAQFNTEKLLWLNQHYMKQLP
PAELAAKVQARLTARGVDTANGPDLEKAVVLYVDRSNTLNVLADAVEVFYAHVTPNPELLAQHLADDARPALAEFAAGIA
TVTWEAPAINALIKETVTKHGLKMPKLAMPLRVILTGQAQTPAVDAVIALIGRDKVAATLAAYL

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=484, Percent_Identity=32.0247933884297, Blast_Score=231, Evalue=8e-61,
Organism=Escherichia coli, GI1788743, Length=454, Percent_Identity=56.1674008810573, Blast_Score=532, Evalue=1e-152,
Organism=Escherichia coli, GI226510925, Length=285, Percent_Identity=35.4385964912281, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1786895, Length=218, Percent_Identity=27.9816513761468, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=465, Percent_Identity=29.6774193548387, Blast_Score=197, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6324540, Length=481, Percent_Identity=29.3139293139293, Blast_Score=215, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6324742, Length=114, Percent_Identity=35.0877192982456, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6321192, Length=236, Percent_Identity=26.6949152542373, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24665321, Length=473, Percent_Identity=31.7124735729387, Blast_Score=230, Evalue=1e-60,
Organism=Drosophila melanogaster, GI17864488, Length=294, Percent_Identity=25.5102040816327, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_DECAR (Q47GG0)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_284541.1
- ProteinModelPortal:   Q47GG0
- SMR:   Q47GG0
- STRING:   Q47GG0
- GeneID:   3569190
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_1321
- NMPDR:   fig|159087.4.peg.1076
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- BioCyc:   DARO159087:DARO_1321-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 51555; Mature: 51555

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 245-245

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPVRTRFAPSPTGYLHIGGARTALFSWAYARRHGGDFILRIEDTDVARSTPEAVQAILD
CCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHH
GMQWLGLEHDEGPFYQMQRMDRYKEVIQQMLANGSAYYCYTTREELDALRAEQEAKKEKP
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHCCC
RYDGRWRPEAGKALPVPPTDVPPVIRFKNPQGGVVAWDDQVKGRIEFANTELDDLIIARA
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCEEEECCCCCCCEEEEEC
DGTPTYNFCVCVDDWDMGITHVIRGDDHVNNTPRQINILKALGAEVPTYAHLSMILGDDG
CCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCHHEEEEEECCCC
AKLSKRHGAVSVMQYDEEGFLTEAVINYLARLGWSHGDDEVFSRQQFVEWFDLDHITASA
CHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AQFNTEKLLWLNQHYMKQLPPAELAAKVQARLTARGVDTANGPDLEKAVVLYVDRSNTLN
HHCCCCCEEEECHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCHH
VLADAVEVFYAHVTPNPELLAQHLADDARPALAEFAAGIATVTWEAPAINALIKETVTKH
HHHHHHHHHEEECCCCHHHHHHHHHCCCCHHHHHHHCCCEEEEECCHHHHHHHHHHHHHC
GLKMPKLAMPLRVILTGQAQTPAVDAVIALIGRDKVAATLAAYL
CCCCCCHHCCEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHC
>Mature Secondary Structure
MKPVRTRFAPSPTGYLHIGGARTALFSWAYARRHGGDFILRIEDTDVARSTPEAVQAILD
CCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHH
GMQWLGLEHDEGPFYQMQRMDRYKEVIQQMLANGSAYYCYTTREELDALRAEQEAKKEKP
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHCCC
RYDGRWRPEAGKALPVPPTDVPPVIRFKNPQGGVVAWDDQVKGRIEFANTELDDLIIARA
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCEEEECCCCCCCEEEEEC
DGTPTYNFCVCVDDWDMGITHVIRGDDHVNNTPRQINILKALGAEVPTYAHLSMILGDDG
CCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCHHEEEEEECCCC
AKLSKRHGAVSVMQYDEEGFLTEAVINYLARLGWSHGDDEVFSRQQFVEWFDLDHITASA
CHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AQFNTEKLLWLNQHYMKQLPPAELAAKVQARLTARGVDTANGPDLEKAVVLYVDRSNTLN
HHCCCCCEEEECHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCHH
VLADAVEVFYAHVTPNPELLAQHLADDARPALAEFAAGIATVTWEAPAINALIKETVTKH
HHHHHHHHHEEECCCCHHHHHHHHHCCCCHHHHHHHCCCEEEEECCHHHHHHHHHHHHHC
GLKMPKLAMPLRVILTGQAQTPAVDAVIALIGRDKVAATLAAYL
CCCCCCHHCCEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA