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The map label for this gene is mltD [H]

Identifier: 71906953

GI number: 71906953

Start: 1436224

End: 1437762

Strand: Reverse

Name: mltD [H]

Synonym: Daro_1320

Alternate gene names: 71906953

Gene position: 1437762-1436224 (Counterclockwise)

Preceding gene: 71906954

Following gene: 71906946

Centisome position: 31.94

GC content: 59.71

Gene sequence:

>1539_bases
ATGCAGCAACGTAACCTTTCGTCGTTTCTTCGCCGTCTGTGCATTACCAGCCTGACCGCGGCAATCCTGCTTCCAGTTGC
TCCCGCTCTAGCTGAGCCAGCAGATAAACCATCCCTGTTTTCCCTGATGATGCCGTCGCTCAGCGATACCGGTTTCAGCG
CAACCGAAGACGTGCTGACCGTGCAGGAAATTCGGGTTCCGCCTCAGCTGCCAAACACGATTGATCTGACCACCCAGGCA
ACCAACCTGTGGGACAGGATTCGCAATGGCTTTGCGATGACCAATCTCAACGATGATCTGGTCCTCTATTACCAGCAGTG
GTACCAGAACCGCCCAGATGCCCTGCGGCGCATGGTCGAGCGCAGCCGCCCCTACCTGCACTATATCGTCGAGGAACTTG
AGGCCCGCGGCATGCCGACCGAGCTGGCATTATTGCCGATGGTTGAAAGCTCCTTCAACCCGAACGCCTATTCGCCGGCA
CACGCGGCCGGCCTGTGGCAGTTCATTCCAGCGACCGGCAAGCGTTTTAATCTGGAGCAAAACTGGTGGCGTGACGAGCG
GCGCGATATCGTTGCCTCGACCGGGGCAGCACTGGACTACCTGCAAGCCATCTACGAAATGCACGGCGACTGGCATCTGG
CGCTGGCTTCCTACAACTGGGGCGAAGGCGCAGTCAAAAGGGCCATCGTCAAGAACGAATCACGCGGCCTGCCGGCAGAC
TATCCAAACCTGACGATGCCGAACGAAACCCGGCATTACGTTCCGAAACTGCAGGCGCTAAAGAATATTCTCGGCAACCC
GACATTGATTGCCCAGCTCGGCCTGCCCTTTATTCCCAATGAGCCGTACTTCGCAACCCTCGAAACTCGGCGGCCAATTG
ATGTCAAGACAGCAGCCCGCCTGGCCAACATGTCGCTGGACGAGTTTCTGCGACTGAACCCCTCGCACAACCGCCCGATC
ATCAAGGCCGACACCTCGGTCGTCGTCCCGGCCGACAAGCTCGACACCTTCCGCGACAACCTGCGAAACAATCAGTCGTC
GCTCACCCAGTGGCAAGCCTACACAGTAACTTCCGCCGAACGGCTGGACAAGATTGCTGCCCGTTTCGATACCACGGCCG
GCGAACTGGCCCGCGTCAATAGCCTCTCGGAAAAGGTTCGGCTCGGCGCCGGCTCAACGGTACTGGTACCTGGCGGCGAT
AGCGGCAGCGGTCTGAGCAACCTGCTCAATACCTCGACCCCGCAACACTTTATCGAGCCGGAACGGGTTGCCCGTCCGTG
TGGCAAGGCTGGCAAGCACGCCAAGTCTTCGGCCAGAGCATGCGACAGACCGGAATCCCGGATCGCTACCAAAGGCAGCC
ACTCAAGCGAGCGTGCAGCGACCAAGGCTTCTGGCAAATCGGCAAAAAACGGTCCGGCCCCAAGCAAGGCACACACCAAG
GCGGCCGAACACGGCAAGACAAAGCCCGCCAGCAGCAAGCCCGTCAAGAGCAGCCCATCCCACCAGCTCTCCAAATCAGG
ACCCAGCCGGCACCGCTGA

Upstream 100 bases:

>100_bases
CTCCGGCTGGTTTTTTTGTTCCTGCTCGGAAAAGTGCTACCCACAGCACCGGGGTATAATCGAGGTTTTCGTCGACCGGT
TCGTGCCCAAGCCCCAATTC

Downstream 100 bases:

>100_bases
TTCAACCCAGCAGATGACAGCTGACAGCGTGGGTGGCTGAAATCTCGGTCGCCTGCGGATAGCGTTGCCGGCAAACATCC
ATTGCCTGAGGACAACGTGG

Product: peptidoglycan-binding LysM:lytic transglycosylase, catalytic

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MQQRNLSSFLRRLCITSLTAAILLPVAPALAEPADKPSLFSLMMPSLSDTGFSATEDVLTVQEIRVPPQLPNTIDLTTQA
TNLWDRIRNGFAMTNLNDDLVLYYQQWYQNRPDALRRMVERSRPYLHYIVEELEARGMPTELALLPMVESSFNPNAYSPA
HAAGLWQFIPATGKRFNLEQNWWRDERRDIVASTGAALDYLQAIYEMHGDWHLALASYNWGEGAVKRAIVKNESRGLPAD
YPNLTMPNETRHYVPKLQALKNILGNPTLIAQLGLPFIPNEPYFATLETRRPIDVKTAARLANMSLDEFLRLNPSHNRPI
IKADTSVVVPADKLDTFRDNLRNNQSSLTQWQAYTVTSAERLDKIAARFDTTAGELARVNSLSEKVRLGAGSTVLVPGGD
SGSGLSNLLNTSTPQHFIEPERVARPCGKAGKHAKSSARACDRPESRIATKGSHSSERAATKASGKSAKNGPAPSKAHTK
AAEHGKTKPASSKPVKSSPSHQLSKSGPSRHR

Sequences:

>Translated_512_residues
MQQRNLSSFLRRLCITSLTAAILLPVAPALAEPADKPSLFSLMMPSLSDTGFSATEDVLTVQEIRVPPQLPNTIDLTTQA
TNLWDRIRNGFAMTNLNDDLVLYYQQWYQNRPDALRRMVERSRPYLHYIVEELEARGMPTELALLPMVESSFNPNAYSPA
HAAGLWQFIPATGKRFNLEQNWWRDERRDIVASTGAALDYLQAIYEMHGDWHLALASYNWGEGAVKRAIVKNESRGLPAD
YPNLTMPNETRHYVPKLQALKNILGNPTLIAQLGLPFIPNEPYFATLETRRPIDVKTAARLANMSLDEFLRLNPSHNRPI
IKADTSVVVPADKLDTFRDNLRNNQSSLTQWQAYTVTSAERLDKIAARFDTTAGELARVNSLSEKVRLGAGSTVLVPGGD
SGSGLSNLLNTSTPQHFIEPERVARPCGKAGKHAKSSARACDRPESRIATKGSHSSERAATKASGKSAKNGPAPSKAHTK
AAEHGKTKPASSKPVKSSPSHQLSKSGPSRHR
>Mature_512_residues
MQQRNLSSFLRRLCITSLTAAILLPVAPALAEPADKPSLFSLMMPSLSDTGFSATEDVLTVQEIRVPPQLPNTIDLTTQA
TNLWDRIRNGFAMTNLNDDLVLYYQQWYQNRPDALRRMVERSRPYLHYIVEELEARGMPTELALLPMVESSFNPNAYSPA
HAAGLWQFIPATGKRFNLEQNWWRDERRDIVASTGAALDYLQAIYEMHGDWHLALASYNWGEGAVKRAIVKNESRGLPAD
YPNLTMPNETRHYVPKLQALKNILGNPTLIAQLGLPFIPNEPYFATLETRRPIDVKTAARLANMSLDEFLRLNPSHNRPI
IKADTSVVVPADKLDTFRDNLRNNQSSLTQWQAYTVTSAERLDKIAARFDTTAGELARVNSLSEKVRLGAGSTVLVPGGD
SGSGLSNLLNTSTPQHFIEPERVARPCGKAGKHAKSSARACDRPESRIATKGSHSSERAATKASGKSAKNGPAPSKAHTK
AAEHGKTKPASSKPVKSSPSHQLSKSGPSRHR

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=340, Percent_Identity=33.8235294117647, Blast_Score=186, Evalue=3e-48,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 56542; Mature: 56542

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQRNLSSFLRRLCITSLTAAILLPVAPALAEPADKPSLFSLMMPSLSDTGFSATEDVLT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCHHHHHH
VQEIRVPPQLPNTIDLTTQATNLWDRIRNGFAMTNLNDDLVLYYQQWYQNRPDALRRMVE
HHHHCCCCCCCCCEEECHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHCCCHHHHHHHHH
RSRPYLHYIVEELEARGMPTELALLPMVESSFNPNAYSPAHAAGLWQFIPATGKRFNLEQ
HCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEECCCH
NWWRDERRDIVASTGAALDYLQAIYEMHGDWHLALASYNWGEGAVKRAIVKNESRGLPAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCC
YPNLTMPNETRHYVPKLQALKNILGNPTLIAQLGLPFIPNEPYFATLETRRPIDVKTAAR
CCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHH
LANMSLDEFLRLNPSHNRPIIKADTSVVVPADKLDTFRDNLRNNQSSLTQWQAYTVTSAE
HHCCCHHHHHHCCCCCCCCEEEECCEEEECHHHHHHHHHHHCCCHHHHHHHHEEEECCHH
RLDKIAARFDTTAGELARVNSLSEKVRLGAGSTVLVPGGDSGSGLSNLLNTSTPQHFIEP
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCHHHCCH
ERVARPCGKAGKHAKSSARACDRPESRIATKGSHSSERAATKASGKSAKNGPAPSKAHTK
HHHHHHHHHCCCHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHH
AAEHGKTKPASSKPVKSSPSHQLSKSGPSRHR
HHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure
MQQRNLSSFLRRLCITSLTAAILLPVAPALAEPADKPSLFSLMMPSLSDTGFSATEDVLT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCHHHHHH
VQEIRVPPQLPNTIDLTTQATNLWDRIRNGFAMTNLNDDLVLYYQQWYQNRPDALRRMVE
HHHHCCCCCCCCCEEECHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHCCCHHHHHHHHH
RSRPYLHYIVEELEARGMPTELALLPMVESSFNPNAYSPAHAAGLWQFIPATGKRFNLEQ
HCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEECCCH
NWWRDERRDIVASTGAALDYLQAIYEMHGDWHLALASYNWGEGAVKRAIVKNESRGLPAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCC
YPNLTMPNETRHYVPKLQALKNILGNPTLIAQLGLPFIPNEPYFATLETRRPIDVKTAAR
CCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHH
LANMSLDEFLRLNPSHNRPIIKADTSVVVPADKLDTFRDNLRNNQSSLTQWQAYTVTSAE
HHCCCHHHHHHCCCCCCCCEEEECCEEEECHHHHHHHHHHHCCCHHHHHHHHEEEECCHH
RLDKIAARFDTTAGELARVNSLSEKVRLGAGSTVLVPGGDSGSGLSNLLNTSTPQHFIEP
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCHHHCCH
ERVARPCGKAGKHAKSSARACDRPESRIATKGSHSSERAATKASGKSAKNGPAPSKAHTK
HHHHHHHHHCCCHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHH
AAEHGKTKPASSKPVKSSPSHQLSKSGPSRHR
HHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]