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The map label for this gene is stp [H]

Identifier: 71906716

GI number: 71906716

Start: 1179975

End: 1181186

Strand: Reverse

Name: stp [H]

Synonym: Daro_1077

Alternate gene names: 71906716

Gene position: 1181186-1179975 (Counterclockwise)

Preceding gene: 71906717

Following gene: 71906715

Centisome position: 26.24

GC content: 62.46

Gene sequence:

>1212_bases
ATGACCACCACGCTTTCTCCCCGTGCCGAACGTTTCTTCCTGCTGACGCTGGCCGGCATCCAGTTCAGCCACGTGCTGGA
CTTCATGATCATGATGCCGCTCGGGCCGGTCCTGATGGCGGCCTTTGGCATCGGCACGCACGAGTTCGGGCTGCTGGTGG
CCTCCTACAGTTTCAGTGCGGCGGTTTCCGGGATACTGGCGGCGACCTTTGTCGACCTGTTCGAGCGCAAGCGGATGCTG
CTGGCCAGCTTCACCCTGTTTGCGCTGGCGACGCTGGCTTGCGGCCTGGCGCCGAGTTATTCGATGCTGATGCTGGCGCG
CGGTCTGGCCGGGATCTTTGGCGGCATCATGGGTGCACTGGTGCATACGATGATCGGCGATGCCATTCCTTTTTCCCGGC
GAGCCAAGGCAAGCGGCATAGTTTCCAGCGCCTTTTCGGTGTCGACGATTGCCGGTGTGCCTTTGTCGCTGTGGCTGGCC
AACCATCTCGGCTGGCGGGCGCCGTTCATGTTCATCGCTACGCTGAGCGTGGGCTTTCTGGCCATCGGCCTGCGCTTCCT
GCCGGAGTTGCGGCACCACCTGAGCGACGAGAAGCGCGCCCATCTGTTCTCGGCCACCTTCAGCGTGCTGGGTGACAGCA
ACCATCTGCGCGCCCTGCTGTTTTCGGCGCTGATCATTTTTTCGGGCTTTACGGTCATTCCCTACATCACCGTCTATGCA
GTCAATAACGTCGGCATCGCCCAGCAGGACATTCCCATCGTGTATCTGGTCGGCGGTTCTGCCACCTTCATCAGCGCCCG
GCTGATCGGGCATTGGGCCGACAAGCACGGCAAGATCGAGGTTTACCGCCGGGTAGCGCTGCTCGCCATGCTGCCCTTGC
TGGCCGTCACGCATGTCGGGGTACTGCCGCTATGGATCTGGCTGATCTGTACCACCAGCTTTTTCGTGCTGACCTCGGGG
CGGATGATCCCGGCCATGGCGATCATCTCTTCAGCTGCCCAGCCCAAGCTGCGCGGCACTTTCATGTCGCTGAACGGCAC
CGTGCAATCGCTGGCCATGGGCCTGGCCACCACGCTGGCCGGGTTTGTCACCTCGCTCGATGCCAGCGGCCGCATCGTCG
ACTACCCCTTGGTCGGCTATGTGGCAGTCGCGGCCAATCTGCTGGCGGTATGGTTTGCCTCCCGCATCGTTATGCACGGG
CGGCATGCCTAG

Upstream 100 bases:

>100_bases
ACTGCGCTTTCCTTGAATTATTCAGCGCCACGCGGTCGAAGCTATCAACTGCCGTCCCGGGCAGCGTATCCTTGGCGCCC
ACGCCAGACTTTGCCGCCCC

Downstream 100 bases:

>100_bases
GCACGGCAGTCCGAAATCCGGAAGCCGCCTTGTTTACCCGGAAAATACTGTATAAACTACTGTATATCGTATCACCGGAG
ACGCCGCCATGTGCGGAAGC

Product: major facilitator transporter

Products: Proton [Cytoplasm]; N-acetylneuraminate [Cytoplasm] [C]

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MTTTLSPRAERFFLLTLAGIQFSHVLDFMIMMPLGPVLMAAFGIGTHEFGLLVASYSFSAAVSGILAATFVDLFERKRML
LASFTLFALATLACGLAPSYSMLMLARGLAGIFGGIMGALVHTMIGDAIPFSRRAKASGIVSSAFSVSTIAGVPLSLWLA
NHLGWRAPFMFIATLSVGFLAIGLRFLPELRHHLSDEKRAHLFSATFSVLGDSNHLRALLFSALIIFSGFTVIPYITVYA
VNNVGIAQQDIPIVYLVGGSATFISARLIGHWADKHGKIEVYRRVALLAMLPLLAVTHVGVLPLWIWLICTTSFFVLTSG
RMIPAMAIISSAAQPKLRGTFMSLNGTVQSLAMGLATTLAGFVTSLDASGRIVDYPLVGYVAVAANLLAVWFASRIVMHG
RHA

Sequences:

>Translated_403_residues
MTTTLSPRAERFFLLTLAGIQFSHVLDFMIMMPLGPVLMAAFGIGTHEFGLLVASYSFSAAVSGILAATFVDLFERKRML
LASFTLFALATLACGLAPSYSMLMLARGLAGIFGGIMGALVHTMIGDAIPFSRRAKASGIVSSAFSVSTIAGVPLSLWLA
NHLGWRAPFMFIATLSVGFLAIGLRFLPELRHHLSDEKRAHLFSATFSVLGDSNHLRALLFSALIIFSGFTVIPYITVYA
VNNVGIAQQDIPIVYLVGGSATFISARLIGHWADKHGKIEVYRRVALLAMLPLLAVTHVGVLPLWIWLICTTSFFVLTSG
RMIPAMAIISSAAQPKLRGTFMSLNGTVQSLAMGLATTLAGFVTSLDASGRIVDYPLVGYVAVAANLLAVWFASRIVMHG
RHA
>Mature_402_residues
TTTLSPRAERFFLLTLAGIQFSHVLDFMIMMPLGPVLMAAFGIGTHEFGLLVASYSFSAAVSGILAATFVDLFERKRMLL
ASFTLFALATLACGLAPSYSMLMLARGLAGIFGGIMGALVHTMIGDAIPFSRRAKASGIVSSAFSVSTIAGVPLSLWLAN
HLGWRAPFMFIATLSVGFLAIGLRFLPELRHHLSDEKRAHLFSATFSVLGDSNHLRALLFSALIIFSGFTVIPYITVYAV
NNVGIAQQDIPIVYLVGGSATFISARLIGHWADKHGKIEVYRRVALLAMLPLLAVTHVGVLPLWIWLICTTSFFVLTSGR
MIPAMAIISSAAQPKLRGTFMSLNGTVQSLAMGLATTLAGFVTSLDASGRIVDYPLVGYVAVAANLLAVWFASRIVMHGR
HA

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87082231, Length=191, Percent_Identity=28.2722513089005, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI1787947, Length=210, Percent_Identity=26.1904761904762, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43242; Mature: 43111

Theoretical pI: Translated: 10.51; Mature: 10.51

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TTTLSPRAERFFLLTLAGIQFSHVLDFMIMMPLGPVLMAAFGIGTHEFGLLVASYSFSA
CCCCCCCHHHEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
AVSGILAATFVDLFERKRMLLASFTLFALATLACGLAPSYSMLMLARGLAGIFGGIMGAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
VHTMIGDAIPFSRRAKASGIVSSAFSVSTIAGVPLSLWLANHLGWRAPFMFIATLSVGFL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
AIGLRFLPELRHHLSDEKRAHLFSATFSVLGDSNHLRALLFSALIIFSGFTVIPYITVYA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHEE
VNNVGIAQQDIPIVYLVGGSATFISARLIGHWADKHGKIEVYRRVALLAMLPLLAVTHVG
ECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
VLPLWIWLICTTSFFVLTSGRMIPAMAIISSAAQPKLRGTFMSLNGTVQSLAMGLATTLA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHH
GFVTSLDASGRIVDYPLVGYVAVAANLLAVWFASRIVMHGRHA
HHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; N-acetylneuraminate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + N-acetylneuraminate [Periplasm] = Proton [Cytoplasm] + N-acetylneuraminate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]