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The map label for this gene is speE [H]

Identifier: 71906475

GI number: 71906475

Start: 907662

End: 908438

Strand: Reverse

Name: speE [H]

Synonym: Daro_0836

Alternate gene names: 71906475

Gene position: 908438-907662 (Counterclockwise)

Preceding gene: 71906479

Following gene: 71906474

Centisome position: 20.18

GC content: 59.85

Gene sequence:

>777_bases
ATGCGACCCAAAAGAAAAACCACCCAGCACTCTGTCGATATCAGCGAAGAAGCCGGCGTGCGTTATCTGCACTTCGGCTC
GGACTGGATTCAGGGCGCCATGCGCATTGCGCGCCCGTGGTCGCTGGAACTGGCCTATACCCGCGAAATGATGGCCGGGC
TGTTGCTCCGCCCCCTGCACCATTGGCCGCGCCATGTGTTGCTGATCGGCCTCGGCGCAGGCTCCCTGGCCAAGTTCATC
TATCGCTATCTGCCCAACTGCCGGATTACGGTGGTCGAGATCAATCCACAGGTTGAATTCATTGCCCGGCAATACTTCAA
ATTACCGGACGATCCGCTACGCCTCGATGTCAGCATAGGCTGCGGCGCCGATTTCATGCTGGGCGGCACGAGCCGCTTCG
ACCTGATCCTGATCGACGGCTACGACCCTGAGGGGCGGGCCGGTGTGCTGGATACCGAGCCGTTCTACCAAGCCTGTCGC
GCCCGGCTGAGTGACAACGGTCTGTGTGGACTGAACCTGCTCGGCTGGAACAGAGGCTTTCAGGGAAGCGTCGAACGTAT
CCGGAGCGCCTTCGACGAGCGAGTCGCCGTTTTCCCCTCGTGCGATAGCGGCAACACCATCGCTTTTGCTACTGGTGGCA
ATCCGGTCGATGTATCGCTGGATGACATGCGCGCCTGCGCCGTGCAACTAAAAAAAGAAACCCGCCTCGACCTTTTGCCA
ACCCTCAGCCGGATACAGTTCGCGTATCCCTTGCCAGACGGTCGTCTCCAGATCTGA

Upstream 100 bases:

>100_bases
CTCCATAGTTAAATGGATATAACGGCTCCCTCCTAAGGAGCAGTTCCTGGTTCGATTCCGGGTGGGGACGCCACCTACCC
AGGCCAGTCGGCGGAGAGCC

Downstream 100 bases:

>100_bases
TTACGTGTATTTCTTGATTTTTCTGTTTTTTGAACTAAAAACAGATTATCCCCACCCATAACGACGACAAGGCGGATCCC
AGAAAAATAATCAGCAGGCA

Product: hypothetical protein

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MRPKRKTTQHSVDISEEAGVRYLHFGSDWIQGAMRIARPWSLELAYTREMMAGLLLRPLHHWPRHVLLIGLGAGSLAKFI
YRYLPNCRITVVEINPQVEFIARQYFKLPDDPLRLDVSIGCGADFMLGGTSRFDLILIDGYDPEGRAGVLDTEPFYQACR
ARLSDNGLCGLNLLGWNRGFQGSVERIRSAFDERVAVFPSCDSGNTIAFATGGNPVDVSLDDMRACAVQLKKETRLDLLP
TLSRIQFAYPLPDGRLQI

Sequences:

>Translated_258_residues
MRPKRKTTQHSVDISEEAGVRYLHFGSDWIQGAMRIARPWSLELAYTREMMAGLLLRPLHHWPRHVLLIGLGAGSLAKFI
YRYLPNCRITVVEINPQVEFIARQYFKLPDDPLRLDVSIGCGADFMLGGTSRFDLILIDGYDPEGRAGVLDTEPFYQACR
ARLSDNGLCGLNLLGWNRGFQGSVERIRSAFDERVAVFPSCDSGNTIAFATGGNPVDVSLDDMRACAVQLKKETRLDLLP
TLSRIQFAYPLPDGRLQI
>Mature_258_residues
MRPKRKTTQHSVDISEEAGVRYLHFGSDWIQGAMRIARPWSLELAYTREMMAGLLLRPLHHWPRHVLLIGLGAGSLAKFI
YRYLPNCRITVVEINPQVEFIARQYFKLPDDPLRLDVSIGCGADFMLGGTSRFDLILIDGYDPEGRAGVLDTEPFYQACR
ARLSDNGLCGLNLLGWNRGFQGSVERIRSAFDERVAVFPSCDSGNTIAFATGGNPVDVSLDDMRACAVQLKKETRLDLLP
TLSRIQFAYPLPDGRLQI

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 28944; Mature: 28944

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPKRKTTQHSVDISEEAGVRYLHFGSDWIQGAMRIARPWSLELAYTREMMAGLLLRPLH
CCCCCCCCCCCCCCCHHCCEEEEEECCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHH
HWPRHVLLIGLGAGSLAKFIYRYLPNCRITVVEINPQVEFIARQYFKLPDDPLRLDVSIG
HCCCEEEEEEECCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCCCCEEEEEEEC
CGADFMLGGTSRFDLILIDGYDPEGRAGVLDTEPFYQACRARLSDNGLCGLNLLGWNRGF
CCCCEEECCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCC
QGSVERIRSAFDERVAVFPSCDSGNTIAFATGGNPVDVSLDDMRACAVQLKKETRLDLLP
CHHHHHHHHHHHCCEEEEECCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHCCHHHHC
TLSRIQFAYPLPDGRLQI
CHHHEEEEECCCCCCEEC
>Mature Secondary Structure
MRPKRKTTQHSVDISEEAGVRYLHFGSDWIQGAMRIARPWSLELAYTREMMAGLLLRPLH
CCCCCCCCCCCCCCCHHCCEEEEEECCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHH
HWPRHVLLIGLGAGSLAKFIYRYLPNCRITVVEINPQVEFIARQYFKLPDDPLRLDVSIG
HCCCEEEEEEECCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCCCCEEEEEEEC
CGADFMLGGTSRFDLILIDGYDPEGRAGVLDTEPFYQACRARLSDNGLCGLNLLGWNRGF
CCCCEEECCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCC
QGSVERIRSAFDERVAVFPSCDSGNTIAFATGGNPVDVSLDDMRACAVQLKKETRLDLLP
CHHHHHHHHHHHCCEEEEECCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHCCHHHHC
TLSRIQFAYPLPDGRLQI
CHHHEEEEECCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]