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The map label for this gene is dosC [H]

Identifier: 71906426

GI number: 71906426

Start: 854055

End: 855812

Strand: Reverse

Name: dosC [H]

Synonym: Daro_0787

Alternate gene names: 71906426

Gene position: 855812-854055 (Counterclockwise)

Preceding gene: 71906428

Following gene: 71906423

Centisome position: 19.01

GC content: 55.97

Gene sequence:

>1758_bases
ATGACCACACTCACCAACCCTTTCGAAATCGCCCGGGAGACATTGCGCCTGCTGGCAACCCGAAGAATCCCCCCCTCTCC
GGACAACTATCTCACCCTCTACCACGAGATTGCCGGCACAAAACCAACCGAAAATTCGTTTCCCGAATCTCAACTACGCT
CGCTGGCAGCAGCCTTACCGAAAGCATCACCAGATCAGCTTCGCCTGGCCCGGCAATTGGACGAGGCTGTCAAGGCAGCC
AACTGGGAGGACTTTAAAAAGGCACTGACTGATTTCATCTCAGCCCTCGCTGAATCGCAAAAACTGAGTTGGTCTGAACT
CATTACTGACTTGCTGCGCCAGTGGGATGCCAAGCACCCAGGGCTGACCGCTGCCCGAAAACGCGAATCACTGGAACATA
TACTGACCAGTAGCGGCGCCAATCCGGATATCCTGTTCAACCGACTGCAAAGCCTTTTGCGCGCATGGGGTCACGGCAAG
GAGACAGAGGCCGCCTCCCTGTCCGAAGAAGCAGCCTCGGAGACAACCCCAACACGCGCATTAACCGCAGCAACGAGTGA
TCTGCTACCTGAATTACGCAAGCTGTTTGCCTTTACCCTCGAAACCGCCATCGCCACCCAGTTACTCGAAAGCCCCCAAC
TCTCGAGCGATGCAAAAGTACTGGCTAACGACATCCGTACTGCCGTCAGCCCGGCACAGTTGCAGGAATTCCTCACTCGC
CTCAAGCGCTTTGCTTTCAAACTTGAATTGCTCGCCGAAGACCAGGCCGAGCTACGCCACAGTCTCCTTAACCTGCTCCG
CCTGCTCGTTGGCAACATCACAGAACTCGTCCTCGATGACCACTGGCTGCACGGACAAATCGCCGTCGTGCGTGAAATCA
TCGACAAGCCGCTCTCCCAGCGCGCCCTCGACGATGCTGAAAGCCGCCTGAAAGAAGTACTGTTCAAGCAGGCACAACTC
AAGGCCAGCCTGTCCGAAGCCCGCGACGCCATCAAGCACATGCTCGCCGGCTTTGTCGACCATCTGGCCGACTTCGCAGA
CGCCACCTCCGACTACCACGACAAGATTGAAAGTTGCGCTGAAAAAATCAGTTCATCCAAAGACATCACCGAACTGGAAG
ACGTCCTTGGCGAAGTCATGCGGGAAACACGCACCATCCAGATCAACGCCCAGCGTTCCCGCGATGAACTACGGTCGACC
CAGCAAAAAGTACAGGAATCAGAAGCGCGGATTCAAGAACTCGAACGCGAACTCGTCGCCAGCAGCGACCTCGTCCGCCA
TGACCAGCTAACCGGCACACTCAACCGCCGAGGGCTGGAAGAAATCTTCACCAAAGAAGCTGCCCGGGCCACCCGCCACG
ACACTGCGCTCTGCGTGGCATTGATCGACATAGACAACTTCAAAAAACTGAACGACTCGATGGGTCACGACACCGGCGAC
CAGGCCCTGATCCATCTCGCCACCATCTGCAGGGAAACCCTCCGCCCGCAGGACACCGTTGCCCGTTACGGTGGCGAAGA
GTTCATCATCCTCCTGCCTGAAACCTCACTGGACGATGCTGCCATCGCCCTCACCCGACTCCAGCGTGAGCTAACCAAAA
AGTTCTTCCTGCATGGTAATGAGAAAGTATTGATCACTTTCAGCGCCGGCGTCACACAAATGCTCCCCAACGACACACAA
GCAACAGTAATCAAACGGGCGGATGAGGCGATGTATCAGGCAAAGAAAACAGGCAAAAACCGGGTTACTACTGCGTAA

Upstream 100 bases:

>100_bases
CGAACCGCTACCTGATGATTACAAATCAACTGCACGACCTTCATGCTACGGGGGCCCAAGCGATGGGCCGCAATTATAGC
CTGATCATCCGAATTTCGAC

Downstream 100 bases:

>100_bases
CAGCCCCTTCGCCTTCGCTACTCGCCCTGAACGCTACAGCAGATAACTCAAAGCCCGGCAGGCGACGAACAAATGCAAAC
CTGAACCCTGCTTCCTTCAG

Product: GGDEF

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 585; Mature: 584

Protein sequence:

>585_residues
MTTLTNPFEIARETLRLLATRRIPPSPDNYLTLYHEIAGTKPTENSFPESQLRSLAAALPKASPDQLRLARQLDEAVKAA
NWEDFKKALTDFISALAESQKLSWSELITDLLRQWDAKHPGLTAARKRESLEHILTSSGANPDILFNRLQSLLRAWGHGK
ETEAASLSEEAASETTPTRALTAATSDLLPELRKLFAFTLETAIATQLLESPQLSSDAKVLANDIRTAVSPAQLQEFLTR
LKRFAFKLELLAEDQAELRHSLLNLLRLLVGNITELVLDDHWLHGQIAVVREIIDKPLSQRALDDAESRLKEVLFKQAQL
KASLSEARDAIKHMLAGFVDHLADFADATSDYHDKIESCAEKISSSKDITELEDVLGEVMRETRTIQINAQRSRDELRST
QQKVQESEARIQELERELVASSDLVRHDQLTGTLNRRGLEEIFTKEAARATRHDTALCVALIDIDNFKKLNDSMGHDTGD
QALIHLATICRETLRPQDTVARYGGEEFIILLPETSLDDAAIALTRLQRELTKKFFLHGNEKVLITFSAGVTQMLPNDTQ
ATVIKRADEAMYQAKKTGKNRVTTA

Sequences:

>Translated_585_residues
MTTLTNPFEIARETLRLLATRRIPPSPDNYLTLYHEIAGTKPTENSFPESQLRSLAAALPKASPDQLRLARQLDEAVKAA
NWEDFKKALTDFISALAESQKLSWSELITDLLRQWDAKHPGLTAARKRESLEHILTSSGANPDILFNRLQSLLRAWGHGK
ETEAASLSEEAASETTPTRALTAATSDLLPELRKLFAFTLETAIATQLLESPQLSSDAKVLANDIRTAVSPAQLQEFLTR
LKRFAFKLELLAEDQAELRHSLLNLLRLLVGNITELVLDDHWLHGQIAVVREIIDKPLSQRALDDAESRLKEVLFKQAQL
KASLSEARDAIKHMLAGFVDHLADFADATSDYHDKIESCAEKISSSKDITELEDVLGEVMRETRTIQINAQRSRDELRST
QQKVQESEARIQELERELVASSDLVRHDQLTGTLNRRGLEEIFTKEAARATRHDTALCVALIDIDNFKKLNDSMGHDTGD
QALIHLATICRETLRPQDTVARYGGEEFIILLPETSLDDAAIALTRLQRELTKKFFLHGNEKVLITFSAGVTQMLPNDTQ
ATVIKRADEAMYQAKKTGKNRVTTA
>Mature_584_residues
TTLTNPFEIARETLRLLATRRIPPSPDNYLTLYHEIAGTKPTENSFPESQLRSLAAALPKASPDQLRLARQLDEAVKAAN
WEDFKKALTDFISALAESQKLSWSELITDLLRQWDAKHPGLTAARKRESLEHILTSSGANPDILFNRLQSLLRAWGHGKE
TEAASLSEEAASETTPTRALTAATSDLLPELRKLFAFTLETAIATQLLESPQLSSDAKVLANDIRTAVSPAQLQEFLTRL
KRFAFKLELLAEDQAELRHSLLNLLRLLVGNITELVLDDHWLHGQIAVVREIIDKPLSQRALDDAESRLKEVLFKQAQLK
ASLSEARDAIKHMLAGFVDHLADFADATSDYHDKIESCAEKISSSKDITELEDVLGEVMRETRTIQINAQRSRDELRSTQ
QKVQESEARIQELERELVASSDLVRHDQLTGTLNRRGLEEIFTKEAARATRHDTALCVALIDIDNFKKLNDSMGHDTGDQ
ALIHLATICRETLRPQDTVARYGGEEFIILLPETSLDDAAIALTRLQRELTKKFFLHGNEKVLITFSAGVTQMLPNDTQA
TVIKRADEAMYQAKKTGKNRVTTA

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=228, Percent_Identity=34.6491228070175, Blast_Score=120, Evalue=4e-28,
Organism=Escherichia coli, GI1786584, Length=187, Percent_Identity=33.1550802139037, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1787816, Length=155, Percent_Identity=40, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI87081881, Length=161, Percent_Identity=39.7515527950311, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1787262, Length=175, Percent_Identity=34.2857142857143, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1788381, Length=186, Percent_Identity=34.9462365591398, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87082007, Length=161, Percent_Identity=35.4037267080745, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1787802, Length=186, Percent_Identity=30.1075268817204, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788085, Length=176, Percent_Identity=34.0909090909091, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI87081974, Length=154, Percent_Identity=30.5194805194805, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI87081977, Length=150, Percent_Identity=31.3333333333333, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1787541, Length=162, Percent_Identity=30.2469135802469, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1788956, Length=188, Percent_Identity=29.2553191489362, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 65475; Mature: 65344

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLTNPFEIARETLRLLATRRIPPSPDNYLTLYHEIAGTKPTENSFPESQLRSLAAALP
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHCCCCCCCCCCCHHHHHHHHHHCC
KASPDQLRLARQLDEAVKAANWEDFKKALTDFISALAESQKLSWSELITDLLRQWDAKHP
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
GLTAARKRESLEHILTSSGANPDILFNRLQSLLRAWGHGKETEAASLSEEAASETTPTRA
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHH
LTAATSDLLPELRKLFAFTLETAIATQLLESPQLSSDAKVLANDIRTAVSPAQLQEFLTR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
LKRFAFKLELLAEDQAELRHSLLNLLRLLVGNITELVLDDHWLHGQIAVVREIIDKPLSQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHH
RALDDAESRLKEVLFKQAQLKASLSEARDAIKHMLAGFVDHLADFADATSDYHDKIESCA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
EKISSSKDITELEDVLGEVMRETRTIQINAQRSRDELRSTQQKVQESEARIQELERELVA
HHHHCCCCHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSDLVRHDQLTGTLNRRGLEEIFTKEAARATRHDTALCVALIDIDNFKKLNDSMGHDTGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHCCCCCHH
QALIHLATICRETLRPQDTVARYGGEEFIILLPETSLDDAAIALTRLQRELTKKFFLHGN
HHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCC
EKVLITFSAGVTQMLPNDTQATVIKRADEAMYQAKKTGKNRVTTA
CEEEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TTLTNPFEIARETLRLLATRRIPPSPDNYLTLYHEIAGTKPTENSFPESQLRSLAAALP
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHCCCCCCCCCCCHHHHHHHHHHCC
KASPDQLRLARQLDEAVKAANWEDFKKALTDFISALAESQKLSWSELITDLLRQWDAKHP
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
GLTAARKRESLEHILTSSGANPDILFNRLQSLLRAWGHGKETEAASLSEEAASETTPTRA
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHH
LTAATSDLLPELRKLFAFTLETAIATQLLESPQLSSDAKVLANDIRTAVSPAQLQEFLTR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
LKRFAFKLELLAEDQAELRHSLLNLLRLLVGNITELVLDDHWLHGQIAVVREIIDKPLSQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHH
RALDDAESRLKEVLFKQAQLKASLSEARDAIKHMLAGFVDHLADFADATSDYHDKIESCA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
EKISSSKDITELEDVLGEVMRETRTIQINAQRSRDELRSTQQKVQESEARIQELERELVA
HHHHCCCCHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSDLVRHDQLTGTLNRRGLEEIFTKEAARATRHDTALCVALIDIDNFKKLNDSMGHDTGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHCCCCCHH
QALIHLATICRETLRPQDTVARYGGEEFIILLPETSLDDAAIALTRLQRELTKKFFLHGN
HHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCC
EKVLITFSAGVTQMLPNDTQATVIKRADEAMYQAKKTGKNRVTTA
CEEEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]