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The map label for this gene is aspS

Identifier: 71906274

GI number: 71906274

Start: 696265

End: 698064

Strand: Reverse

Name: aspS

Synonym: Daro_0634

Alternate gene names: 71906274

Gene position: 698064-696265 (Counterclockwise)

Preceding gene: 71906275

Following gene: 71906273

Centisome position: 15.51

GC content: 61.56

Gene sequence:

>1800_bases
ATGCGTACCCATTATTGCGGACAACTCAATGCCGCCCTTGACGGGCAAATCGTTACCCTGTGCGGCTGGGCCCACCGTCG
ACGCGACCACGGCGGCGTCATCTTCATCGATTTGCGCGACCGCGAAGGCCTCGCCCAGGTGGTTTGCGACCCTGATCGCG
CTGACACCTTCAAGATCGCCGAATCCGTCCGCAACGAGTTCTGCCTGAAGATTACCGGCAAGGTGCGCCCGCGCCCGGCA
GGCACAACCAATGCCAACCTTGCTTCCGGCGAAATTGAAATTCTTTGCCACGAGATCGAAGTTCTGAACCCGTCCGTCAC
GCCGCCGTTCCAGCTGGATGAAGACAATCTCTCCGAGAACGTTCGCCTGTTGCACCGCGTCATCGACCTGCGTCGCCCAC
AGATGCAGAACAACCTGATGCTGCGCTACAAGACATCCCGTGCCTTCCGCCGTTTCTTGGACGACAACGGCTTTATCGAC
ATCGAAACGCCGATGCTGACCAAGTCCACCCCGGAAGGTGCCCGCGACTATCTCGTGCCGTCACGTGTCCACCCCGGCCA
GTTCTTTGCGCTGCCGCAGTCGCCGCAGCTCTTCAAGCAGCTGCTGATGGTCGCTGGCTACGACCGCTATTACCAGATCG
TCAAGTGCTTCCGCGACGAAGACCTGCGCGCTGACCGTCAGCCGGAATTTACCCAGGTCGATATCGAAACCTCGTTCATG
AGCGAGGCCGAGATCATGGCGCTGACTGAAAAGTTGATCCGCACCGTCTTCAAGGAAGCCATCGACGTCGATCTGCCGGA
TTTCCCGCGCATGACCTACGCCGAAGCCATGCACCGCTTCGGCTCCGACAAGCCGGACCTGCGTGTAACGCTGGAACTGA
CTGAAGTCACCGATGCCTTCAAAGACGTCGCCTTCAAGGTCTTCGCCGGCGTTGCCAACAGCGAAGGCGGCCGCATCGCC
GCCATGCGCATCCCGGGCGGCGCCACGCTGACCCGTGGCGAAATCGATGCCTACACTCAGTTCGTCGGTATCTACGGCGC
CAAGGGCCTGGCCTACATCAAGGTCAACGACGCGAGCCAGATCAACGAAACCGGCCTGCAGTCACCGATCGTCAAGAACC
TCTCGGAAGCCTCACTGAAGGCCGTCATCGAGCGCACTGGCGCCCAGTCCGGCGACCTGATTTTCTTCGGCGCCGACAAG
GCCAAGATCGTCAACGATGCCCTCGGCGCGTTGCGCATCAAGATCGGCCACGAAAAGGGCTTCGTCACCGGCGCCAAGTG
GGCTCCGCTGTGGGTCATCGACTTCCCGATGTTCGAGTATGACGACGAATCCAAGCGCTGGACCGCCTGCCACCACCCGT
TCACCAGCCCCAAGGACGAGCATCTCGACCTGCTGGTCAGCGATCCGGGCAAGTGCCTGGCCAAGGCCTACGACCTGGCG
CTGAACGGTTGGGAACTGGGCGGCGGCTCGGTACGTATTCACCGTTCCGACGTGCAGGAGAAGGTCTTCTCAGCCCTCAA
CATCGGTCCGGAAGAACAGCAGGCCAAGTTCGGCTTCCTGCTTGACGCCCTGAAGTACGGCGCCCCGCCGCACGGCGGCC
TGGCTTTCGGCCTCGATCGCATCGTCACGATGATGACCGGCGCCGAGTCGATCCGCGACGTGATTGCCTTCCCGAAGACC
CAGCGCGCCCAGTGCCTGCTGACCGACGCACCGAGCGGTGTCGACGAGAAGCAACTGCGGGAACTGCACATCCGCCTGCG
CCAGAAGGTTGAAACTCAAGTTGAAGTCGGCCAGCCCTAA

Upstream 100 bases:

>100_bases
CGGTCGATGAAGCACTTAAATACATTATCTCGATGGGTGTCGTGGCGCCCCAGCCCCACCCTCGCGTCGTTACCAACACC
TGAATCAACCGGAAAGTTTC

Downstream 100 bases:

>100_bases
GCAAGCATGCTGATCTTTCTGCGCTTCCTGGTCGTTGCCTGGTTGGCGATCGGAAGCGCATTTGCTTTTAGCTTTGACCG
TAGCGCCTCTGCCGATAGCG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MRTHYCGQLNAALDGQIVTLCGWAHRRRDHGGVIFIDLRDREGLAQVVCDPDRADTFKIAESVRNEFCLKITGKVRPRPA
GTTNANLASGEIEILCHEIEVLNPSVTPPFQLDEDNLSENVRLLHRVIDLRRPQMQNNLMLRYKTSRAFRRFLDDNGFID
IETPMLTKSTPEGARDYLVPSRVHPGQFFALPQSPQLFKQLLMVAGYDRYYQIVKCFRDEDLRADRQPEFTQVDIETSFM
SEAEIMALTEKLIRTVFKEAIDVDLPDFPRMTYAEAMHRFGSDKPDLRVTLELTEVTDAFKDVAFKVFAGVANSEGGRIA
AMRIPGGATLTRGEIDAYTQFVGIYGAKGLAYIKVNDASQINETGLQSPIVKNLSEASLKAVIERTGAQSGDLIFFGADK
AKIVNDALGALRIKIGHEKGFVTGAKWAPLWVIDFPMFEYDDESKRWTACHHPFTSPKDEHLDLLVSDPGKCLAKAYDLA
LNGWELGGGSVRIHRSDVQEKVFSALNIGPEEQQAKFGFLLDALKYGAPPHGGLAFGLDRIVTMMTGAESIRDVIAFPKT
QRAQCLLTDAPSGVDEKQLRELHIRLRQKVETQVEVGQP

Sequences:

>Translated_599_residues
MRTHYCGQLNAALDGQIVTLCGWAHRRRDHGGVIFIDLRDREGLAQVVCDPDRADTFKIAESVRNEFCLKITGKVRPRPA
GTTNANLASGEIEILCHEIEVLNPSVTPPFQLDEDNLSENVRLLHRVIDLRRPQMQNNLMLRYKTSRAFRRFLDDNGFID
IETPMLTKSTPEGARDYLVPSRVHPGQFFALPQSPQLFKQLLMVAGYDRYYQIVKCFRDEDLRADRQPEFTQVDIETSFM
SEAEIMALTEKLIRTVFKEAIDVDLPDFPRMTYAEAMHRFGSDKPDLRVTLELTEVTDAFKDVAFKVFAGVANSEGGRIA
AMRIPGGATLTRGEIDAYTQFVGIYGAKGLAYIKVNDASQINETGLQSPIVKNLSEASLKAVIERTGAQSGDLIFFGADK
AKIVNDALGALRIKIGHEKGFVTGAKWAPLWVIDFPMFEYDDESKRWTACHHPFTSPKDEHLDLLVSDPGKCLAKAYDLA
LNGWELGGGSVRIHRSDVQEKVFSALNIGPEEQQAKFGFLLDALKYGAPPHGGLAFGLDRIVTMMTGAESIRDVIAFPKT
QRAQCLLTDAPSGVDEKQLRELHIRLRQKVETQVEVGQP
>Mature_599_residues
MRTHYCGQLNAALDGQIVTLCGWAHRRRDHGGVIFIDLRDREGLAQVVCDPDRADTFKIAESVRNEFCLKITGKVRPRPA
GTTNANLASGEIEILCHEIEVLNPSVTPPFQLDEDNLSENVRLLHRVIDLRRPQMQNNLMLRYKTSRAFRRFLDDNGFID
IETPMLTKSTPEGARDYLVPSRVHPGQFFALPQSPQLFKQLLMVAGYDRYYQIVKCFRDEDLRADRQPEFTQVDIETSFM
SEAEIMALTEKLIRTVFKEAIDVDLPDFPRMTYAEAMHRFGSDKPDLRVTLELTEVTDAFKDVAFKVFAGVANSEGGRIA
AMRIPGGATLTRGEIDAYTQFVGIYGAKGLAYIKVNDASQINETGLQSPIVKNLSEASLKAVIERTGAQSGDLIFFGADK
AKIVNDALGALRIKIGHEKGFVTGAKWAPLWVIDFPMFEYDDESKRWTACHHPFTSPKDEHLDLLVSDPGKCLAKAYDLA
LNGWELGGGSVRIHRSDVQEKVFSALNIGPEEQQAKFGFLLDALKYGAPPHGGLAFGLDRIVTMMTGAESIRDVIAFPKT
QRAQCLLTDAPSGVDEKQLRELHIRLRQKVETQVEVGQP

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=606, Percent_Identity=38.9438943894389, Blast_Score=421, Evalue=1e-117,
Organism=Homo sapiens, GI194272210, Length=285, Percent_Identity=25.9649122807018, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI5031815, Length=285, Percent_Identity=25.9649122807018, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI45439306, Length=301, Percent_Identity=26.2458471760797, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1788173, Length=593, Percent_Identity=59.1905564924115, Blast_Score=729, Evalue=0.0,
Organism=Escherichia coli, GI1790571, Length=308, Percent_Identity=26.2987012987013, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1789256, Length=308, Percent_Identity=25.974025974026, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI32566633, Length=605, Percent_Identity=34.7107438016529, Blast_Score=323, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI71994340, Length=250, Percent_Identity=23.6, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=250, Percent_Identity=23.6, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17535927, Length=250, Percent_Identity=23.6, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17551876, Length=276, Percent_Identity=24.6376811594203, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=312, Percent_Identity=36.8589743589744, Blast_Score=181, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6320242, Length=263, Percent_Identity=24.7148288973384, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323011, Length=270, Percent_Identity=27.7777777777778, Blast_Score=70, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6324256, Length=167, Percent_Identity=29.3413173652695, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24584738, Length=604, Percent_Identity=35.7615894039735, Blast_Score=348, Evalue=5e-96,
Organism=Drosophila melanogaster, GI17136276, Length=273, Percent_Identity=26.007326007326, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24640849, Length=257, Percent_Identity=26.8482490272374, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24640851, Length=257, Percent_Identity=26.8482490272374, Blast_Score=80, Evalue=6e-15,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_DECAR (Q47IE0)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_283861.1
- ProteinModelPortal:   Q47IE0
- SMR:   Q47IE0
- STRING:   Q47IE0
- GeneID:   3568901
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_0634
- NMPDR:   fig|159087.4.peg.523
- eggNOG:   COG0173
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- BioCyc:   DARO159087:DARO_0634-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66957; Mature: 66957

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTHYCGQLNAALDGQIVTLCGWAHRRRDHGGVIFIDLRDREGLAQVVCDPDRADTFKIA
CCCCCCCCCCCCCCCCEEEEECHHHHHCCCCCEEEEEECCCCCCCCEEECCCCCHHHHHH
ESVRNEFCLKITGKVRPRPAGTTNANLASGEIEILCHEIEVLNPSVTPPFQLDEDNLSEN
HHHCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCHHHH
VRLLHRVIDLRRPQMQNNLMLRYKTSRAFRRFLDDNGFIDIETPMLTKSTPEGARDYLVP
HHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHCCCC
SRVHPGQFFALPQSPQLFKQLLMVAGYDRYYQIVKCFRDEDLRADRQPEFTQVDIETSFM
CCCCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHH
SEAEIMALTEKLIRTVFKEAIDVDLPDFPRMTYAEAMHRFGSDKPDLRVTLELTEVTDAF
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEHHHHHHH
KDVAFKVFAGVANSEGGRIAAMRIPGGATLTRGEIDAYTQFVGIYGAKGLAYIKVNDASQ
HHHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCHHHHHHHHHHCCCCCEEEEEECCCCH
INETGLQSPIVKNLSEASLKAVIERTGAQSGDLIFFGADKAKIVNDALGALRIKIGHEKG
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHCEEEEEEECCCC
FVTGAKWAPLWVIDFPMFEYDDESKRWTACHHPFTSPKDEHLDLLVSDPGKCLAKAYDLA
CEECCCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHE
LNGWELGGGSVRIHRSDVQEKVFSALNIGPEEQQAKFGFLLDALKYGAPPHGGLAFGLDR
ECCEEECCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
IVTMMTGAESIRDVIAFPKTQRAQCLLTDAPSGVDEKQLRELHIRLRQKVETQVEVGQP
HHHHHHCHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRTHYCGQLNAALDGQIVTLCGWAHRRRDHGGVIFIDLRDREGLAQVVCDPDRADTFKIA
CCCCCCCCCCCCCCCCEEEEECHHHHHCCCCCEEEEEECCCCCCCCEEECCCCCHHHHHH
ESVRNEFCLKITGKVRPRPAGTTNANLASGEIEILCHEIEVLNPSVTPPFQLDEDNLSEN
HHHCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCHHHH
VRLLHRVIDLRRPQMQNNLMLRYKTSRAFRRFLDDNGFIDIETPMLTKSTPEGARDYLVP
HHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHCCCC
SRVHPGQFFALPQSPQLFKQLLMVAGYDRYYQIVKCFRDEDLRADRQPEFTQVDIETSFM
CCCCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHH
SEAEIMALTEKLIRTVFKEAIDVDLPDFPRMTYAEAMHRFGSDKPDLRVTLELTEVTDAF
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEHHHHHHH
KDVAFKVFAGVANSEGGRIAAMRIPGGATLTRGEIDAYTQFVGIYGAKGLAYIKVNDASQ
HHHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCHHHHHHHHHHCCCCCEEEEEECCCCH
INETGLQSPIVKNLSEASLKAVIERTGAQSGDLIFFGADKAKIVNDALGALRIKIGHEKG
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHCEEEEEEECCCC
FVTGAKWAPLWVIDFPMFEYDDESKRWTACHHPFTSPKDEHLDLLVSDPGKCLAKAYDLA
CEECCCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHE
LNGWELGGGSVRIHRSDVQEKVFSALNIGPEEQQAKFGFLLDALKYGAPPHGGLAFGLDR
ECCEEECCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
IVTMMTGAESIRDVIAFPKTQRAQCLLTDAPSGVDEKQLRELHIRLRQKVETQVEVGQP
HHHHHHCHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA