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The map label for this gene is argB

Identifier: 71906269

GI number: 71906269

Start: 693561

End: 694448

Strand: Reverse

Name: argB

Synonym: Daro_0629

Alternate gene names: 71906269

Gene position: 694448-693561 (Counterclockwise)

Preceding gene: 71906270

Following gene: 71906268

Centisome position: 15.43

GC content: 58.78

Gene sequence:

>888_bases
ATGAGCATTGAAGACCTTTCCCCCGGCACCAAGGCCGCCGTTCTGGCCGAAGCCCTCCCCTACATCAAGCGTTTCCACGG
CAAGACCATCGTTGTCAAATACGGCGGCAACGCGATGACCGACGAGCATCTGAAAAGCTGTTTTGCCCGCGATGTCGTGC
TGCTTGAACTGGTCGGCTTCAACATTGTTGTCGTTCATGGCGGCGGCCCCCAGATCGAAAGCCTGCTTGCCCGTGTCGGC
AAGAAGGGGGAATTCATCCAAGGCATGCGCGTCACCGATGCCGAGACCATGGAAGTTGTCGAAATGGTCTTGGGCGGGCA
GGTCAACAAGGACATCGTCAATCTGATCAACCAGCACGGTGGCAAGGCCGTCGGCCTGACCGGCAAGGATGGCAATTTCA
TCCGCGCCAAGAAGTTGATGCTGGAGGACAAGGACAACCCGGGTGACCTGATCGACGTGGGCCAGGTCGGCGAGATCGTC
TCCATTGATCCGACACTGATCGACCACCTCGACAAGGGGGCCTTCATCCCCGTGATCGCCCCGATCGGGGTCGGCAAGGA
TGGCGAGACCTACAATATCAACGCCGACGTCGTCGCTGGCAAGATCGCCGAAGTCCTCAAGGCCGAGAAGCTGGTCCTGC
TGACCAACACGCCGGGCGTACTCGACAAGAATGGCGATCTGATTACCGGCATCACGCCCAAGCAGATCGACGAAATGGTC
GATGATGGCACACTGTCCGGCGGCATGCTGCCCAAAATCGGCTCCGCGCTTGATGCCGCCCGCAACGGCGTCAAAGGGGT
TCACATCATTGATGGCCGTGTCGAGCATGCGCTACTCCTGGAAATTCTGACCGACCATGGCGTCGGCACAATGATCAAAT
CGCACTGA

Upstream 100 bases:

>100_bases
CTGAATATCGAGTAAGCCAAGCGATTCCAAGCATCAAGGCCGGACCCCGTTCCGGCCTTTTTCTTTGTACAATCCGCGGC
GATTCTACTTGGAGCCCGCC

Downstream 100 bases:

>100_bases
CATCGGCACCAGGAATGAAAAGCCCGCAACTGCGGGCTTTTTCAATTGCACTTAGAGTATCGCGATGAAGAAACCTGTCT
GGCTATTCGACCTCGACAAT

Product: acetylglutamate kinase

Products: NA

Alternate protein names: N-acetyl-L-glutamate 5-phosphotransferase; NAG kinase; AGK

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MSIEDLSPGTKAAVLAEALPYIKRFHGKTIVVKYGGNAMTDEHLKSCFARDVVLLELVGFNIVVVHGGGPQIESLLARVG
KKGEFIQGMRVTDAETMEVVEMVLGGQVNKDIVNLINQHGGKAVGLTGKDGNFIRAKKLMLEDKDNPGDLIDVGQVGEIV
SIDPTLIDHLDKGAFIPVIAPIGVGKDGETYNINADVVAGKIAEVLKAEKLVLLTNTPGVLDKNGDLITGITPKQIDEMV
DDGTLSGGMLPKIGSALDAARNGVKGVHIIDGRVEHALLLEILTDHGVGTMIKSH

Sequences:

>Translated_295_residues
MSIEDLSPGTKAAVLAEALPYIKRFHGKTIVVKYGGNAMTDEHLKSCFARDVVLLELVGFNIVVVHGGGPQIESLLARVG
KKGEFIQGMRVTDAETMEVVEMVLGGQVNKDIVNLINQHGGKAVGLTGKDGNFIRAKKLMLEDKDNPGDLIDVGQVGEIV
SIDPTLIDHLDKGAFIPVIAPIGVGKDGETYNINADVVAGKIAEVLKAEKLVLLTNTPGVLDKNGDLITGITPKQIDEMV
DDGTLSGGMLPKIGSALDAARNGVKGVHIIDGRVEHALLLEILTDHGVGTMIKSH
>Mature_294_residues
SIEDLSPGTKAAVLAEALPYIKRFHGKTIVVKYGGNAMTDEHLKSCFARDVVLLELVGFNIVVVHGGGPQIESLLARVGK
KGEFIQGMRVTDAETMEVVEMVLGGQVNKDIVNLINQHGGKAVGLTGKDGNFIRAKKLMLEDKDNPGDLIDVGQVGEIVS
IDPTLIDHLDKGAFIPVIAPIGVGKDGETYNINADVVAGKIAEVLKAEKLVLLTNTPGVLDKNGDLITGITPKQIDEMVD
DGTLSGGMLPKIGSALDAARNGVKGVHIIDGRVEHALLLEILTDHGVGTMIKSH

Specific function: Arginine biosynthesis; first step. [C]

COG id: COG0548

COG function: function code E; Acetylglutamate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetylglutamate kinase family

Homologues:

Organism=Escherichia coli, GI1789181, Length=283, Percent_Identity=30.3886925795053, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI145693208, Length=245, Percent_Identity=31.0204081632653, Blast_Score=102, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6320913, Length=294, Percent_Identity=30.6122448979592, Blast_Score=86, Evalue=8e-18,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ARGB_DECAR (Q47IE5)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_283856.1
- HSSP:   Q9HTN2
- ProteinModelPortal:   Q47IE5
- SMR:   Q47IE5
- STRING:   Q47IE5
- GeneID:   3568896
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_0629
- NMPDR:   fig|159087.4.peg.518
- eggNOG:   COG0548
- HOGENOM:   HBG497643
- OMA:   MDIVEMV
- ProtClustDB:   PRK00942
- BioCyc:   DARO159087:DARO_0629-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00082_B
- InterPro:   IPR004662
- InterPro:   IPR001048
- InterPro:   IPR001057
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000728
- PRINTS:   PR00474
- TIGRFAMs:   TIGR00761

Pfam domain/function: PF00696 AA_kinase; SSF53633 Aa_kinase

EC number: =2.7.2.8

Molecular weight: Translated: 31214; Mature: 31083

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: BINDING 90-90 BINDING 192-192

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEDLSPGTKAAVLAEALPYIKRFHGKTIVVKYGGNAMTDEHLKSCFARDVVLLELVGF
CCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHEEEECC
NIVVVHGGGPQIESLLARVGKKGEFIQGMRVTDAETMEVVEMVLGGQVNKDIVNLINQHG
EEEEEECCCHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCC
GKAVGLTGKDGNFIRAKKLMLEDKDNPGDLIDVGQVGEIVSIDPTLIDHLDKGAFIPVIA
CEEEEEECCCCCEEEEEHHHCCCCCCCCCEEECCCCCCEEECCHHHHHHCCCCCEEEEEE
PIGVGKDGETYNINADVVAGKIAEVLKAEKLVLLTNTPGVLDKNGDLITGITPKQIDEMV
ECCCCCCCCEEECCHHHHHHHHHHHHHHCCEEEEECCCCCEECCCCEEECCCHHHHHHHH
DDGTLSGGMLPKIGSALDAARNGVKGVHIIDGRVEHALLLEILTDHGVGTMIKSH
CCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SIEDLSPGTKAAVLAEALPYIKRFHGKTIVVKYGGNAMTDEHLKSCFARDVVLLELVGF
CCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHEEEECC
NIVVVHGGGPQIESLLARVGKKGEFIQGMRVTDAETMEVVEMVLGGQVNKDIVNLINQHG
EEEEEECCCHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCC
GKAVGLTGKDGNFIRAKKLMLEDKDNPGDLIDVGQVGEIVSIDPTLIDHLDKGAFIPVIA
CEEEEEECCCCCEEEEEHHHCCCCCCCCCEEECCCCCCEEECCHHHHHHCCCCCEEEEEE
PIGVGKDGETYNINADVVAGKIAEVLKAEKLVLLTNTPGVLDKNGDLITGITPKQIDEMV
ECCCCCCCCEEECCHHHHHHHHHHHHHHCCEEEEECCCCCEECCCCEEECCCHHHHHHHH
DDGTLSGGMLPKIGSALDAARNGVKGVHIIDGRVEHALLLEILTDHGVGTMIKSH
CCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA