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The map label for this gene is dctD [H]

Identifier: 71906117

GI number: 71906117

Start: 537517

End: 538848

Strand: Reverse

Name: dctD [H]

Synonym: Daro_0477

Alternate gene names: 71906117

Gene position: 538848-537517 (Counterclockwise)

Preceding gene: 71906118

Following gene: 71906116

Centisome position: 11.97

GC content: 66.67

Gene sequence:

>1332_bases
ATGACCGACGACCTGAAAATCCTGATCATCGAAGACGACCCCGATGTGGCGCTCGGCTGCGAGCAGGCGCTGCAACTCGA
AGGACTGGCCGCCGAATGCGCCGGCAGCGCCGAACTGGCACGCCACCGCCTGCATGCCGACTTCCCCGGCATCGTGATCA
GCGATATCCGCCTGCCCGGCCAGGATGGCCTGAGCCTGCAGCGCGAACTGCACGCCCTCGACCCGGAATTGCCGGTGCTA
CTGATCACCGGCCACGGCGATGTGTCGATGGCCGTACAGGCGATGAAGGATGGCGCCTACGATTTCCTGCAAAAGCCCTT
TGCCCCCGAGCAGCTCGTCGACATCGCCCGCCGCGCGCTGGAAAAGCGCCGGCTGGTCCTCGAAGTGCGCGCCCTGCGCC
GCCAGCTCGACCAGCGCAATCTGCTCGAAGCCCGCCTGATCGGCAACTCGGCCGGCATGGCCCGCGTCCGTGGCCTGATC
GGCGCCCTGGCCAGCAGCGCGGCCGACGTGCTGATCCACGGTGAGACCGGCACTGGCAAGGAACTGGTTGCCCGCTGCCT
GCACGACGCCAGCCCGCGGGCCAAGGGTAATTTCGTCGCCATCAACTGCGGCGGCCTGCCGGAAACGCTGTTCGAAAGTG
AAATCTTCGGTCACGAGGCGGGTGCCTACACCGGTGCCGGCAAGCGGCGCATCGGCAAGATCGAGTACGCCAACGGCGGC
ACGCTGTTTCTCGACGAAATCGAAACCATGCCGGTCCCGATGCAGATCAAGTTGCTGCGTGTGCTACAGGAGCGCACGTT
GGAGCGGCTCGGGTCGAATACGGCGATTCCCATCGACTGCCGCATCATTGCCGCGACCAAGGAAGACCTGACGGCATTGT
CCGAGCAAGGAAAATTCCGTGCCGACCTCTACTACCGCCTGTCGGTCGCCAACCTGCCGCTGCCACCGCTGCGCGAGCGG
CGCGAGGACATTCCGCTGCTCTTCGAACACTTCCTGCTGCAAGCCGCCGCCCGCCACGAACGCGCCGTGCCTACGACATC
CGCCGGCCGCATGCAGCAACTGGTCGGCTACCACTGGCCGGGCAACGTGCGCGAATTGCGCAACGTCGCCGACCGCTGCG
TGCTCGGCATCGAAGCCGGCTCGCCGCCTTTCGGCCAGCCCGCCGGCGATGCGCCGCGCCCGCTGGCCGAGACCGTCGAC
GCCTTCGAACGCGCCCTGATCGCCGACGCCCTGCGCCGCTATGGCAGCCTGGCGCGCACCGCCGAAGCCCTGCTCATCGC
CAAGACAACACTGCACGACAAGATTCGGAAATACGGTTTGCACGGCGAGTGA

Upstream 100 bases:

>100_bases
CCGCCGGCATCATCCGCGACTTCGGTGGTAGCCTGCACGGGGCCAACCACCCCGACGGTGGCGCCATCTTCACACTCGAA
CTCCCGCTCGCCCCCGCGCC

Downstream 100 bases:

>100_bases
GCCGCCCAAAAGCAAAGGGCAGACCGGCAATGCCGGCCTGCCCTTGTTCCATTCCCCTGCCGGGGCAACGCTTCCGGTAA
AAAGCGCCTGTCCGGACCAA

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MTDDLKILIIEDDPDVALGCEQALQLEGLAAECAGSAELARHRLHADFPGIVISDIRLPGQDGLSLQRELHALDPELPVL
LITGHGDVSMAVQAMKDGAYDFLQKPFAPEQLVDIARRALEKRRLVLEVRALRRQLDQRNLLEARLIGNSAGMARVRGLI
GALASSAADVLIHGETGTGKELVARCLHDASPRAKGNFVAINCGGLPETLFESEIFGHEAGAYTGAGKRRIGKIEYANGG
TLFLDEIETMPVPMQIKLLRVLQERTLERLGSNTAIPIDCRIIAATKEDLTALSEQGKFRADLYYRLSVANLPLPPLRER
REDIPLLFEHFLLQAAARHERAVPTTSAGRMQQLVGYHWPGNVRELRNVADRCVLGIEAGSPPFGQPAGDAPRPLAETVD
AFERALIADALRRYGSLARTAEALLIAKTTLHDKIRKYGLHGE

Sequences:

>Translated_443_residues
MTDDLKILIIEDDPDVALGCEQALQLEGLAAECAGSAELARHRLHADFPGIVISDIRLPGQDGLSLQRELHALDPELPVL
LITGHGDVSMAVQAMKDGAYDFLQKPFAPEQLVDIARRALEKRRLVLEVRALRRQLDQRNLLEARLIGNSAGMARVRGLI
GALASSAADVLIHGETGTGKELVARCLHDASPRAKGNFVAINCGGLPETLFESEIFGHEAGAYTGAGKRRIGKIEYANGG
TLFLDEIETMPVPMQIKLLRVLQERTLERLGSNTAIPIDCRIIAATKEDLTALSEQGKFRADLYYRLSVANLPLPPLRER
REDIPLLFEHFLLQAAARHERAVPTTSAGRMQQLVGYHWPGNVRELRNVADRCVLGIEAGSPPFGQPAGDAPRPLAETVD
AFERALIADALRRYGSLARTAEALLIAKTTLHDKIRKYGLHGE
>Mature_442_residues
TDDLKILIIEDDPDVALGCEQALQLEGLAAECAGSAELARHRLHADFPGIVISDIRLPGQDGLSLQRELHALDPELPVLL
ITGHGDVSMAVQAMKDGAYDFLQKPFAPEQLVDIARRALEKRRLVLEVRALRRQLDQRNLLEARLIGNSAGMARVRGLIG
ALASSAADVLIHGETGTGKELVARCLHDASPRAKGNFVAINCGGLPETLFESEIFGHEAGAYTGAGKRRIGKIEYANGGT
LFLDEIETMPVPMQIKLLRVLQERTLERLGSNTAIPIDCRIIAATKEDLTALSEQGKFRADLYYRLSVANLPLPPLRERR
EDIPLLFEHFLLQAAARHERAVPTTSAGRMQQLVGYHWPGNVRELRNVADRCVLGIEAGSPPFGQPAGDAPRPLAETVDA
FERALIADALRRYGSLARTAEALLIAKTTLHDKIRKYGLHGE

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=457, Percent_Identity=40.9190371991247, Blast_Score=333, Evalue=2e-92,
Organism=Escherichia coli, GI1790437, Length=450, Percent_Identity=38.8888888888889, Blast_Score=295, Evalue=3e-81,
Organism=Escherichia coli, GI1788905, Length=368, Percent_Identity=44.5652173913043, Blast_Score=288, Evalue=4e-79,
Organism=Escherichia coli, GI1790299, Length=401, Percent_Identity=42.3940149625935, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1789087, Length=330, Percent_Identity=43.3333333333333, Blast_Score=239, Evalue=4e-64,
Organism=Escherichia coli, GI87082117, Length=236, Percent_Identity=50, Blast_Score=235, Evalue=5e-63,
Organism=Escherichia coli, GI1789233, Length=319, Percent_Identity=39.4984326018809, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI87082152, Length=338, Percent_Identity=40.2366863905325, Blast_Score=214, Evalue=6e-57,
Organism=Escherichia coli, GI1786524, Length=265, Percent_Identity=45.6603773584906, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI87081872, Length=230, Percent_Identity=46.0869565217391, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1787583, Length=312, Percent_Identity=37.1794871794872, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI87081858, Length=290, Percent_Identity=33.1034482758621, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1789828, Length=260, Percent_Identity=35, Blast_Score=125, Evalue=8e-30,
Organism=Escherichia coli, GI1790552, Length=108, Percent_Identity=36.1111111111111, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789809, Length=123, Percent_Identity=28.4552845528455, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 48437; Mature: 48306

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4 ; PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDDLKILIIEDDPDVALGCEQALQLEGLAAECAGSAELARHRLHADFPGIVISDIRLPG
CCCCEEEEEEECCCCCCCCHHHHHHHCCCHHHHCCHHHHHHHHHCCCCCCEEEEEEECCC
QDGLSLQRELHALDPELPVLLITGHGDVSMAVQAMKDGAYDFLQKPFAPEQLVDIARRAL
CCCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHH
EKRRLVLEVRALRRQLDQRNLLEARLIGNSAGMARVRGLIGALASSAADVLIHGETGTGK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCH
ELVARCLHDASPRAKGNFVAINCGGLPETLFESEIFGHEAGAYTGAGKRRIGKIEYANGG
HHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCC
TLFLDEIETMPVPMQIKLLRVLQERTLERLGSNTAIPIDCRIIAATKEDLTALSEQGKFR
EEEEECHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECHHHHHHHHHCCCEE
ADLYYRLSVANLPLPPLRERREDIPLLFEHFLLQAAARHERAVPTTSAGRMQQLVGYHWP
EEEEEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCC
GNVRELRNVADRCVLGIEAGSPPFGQPAGDAPRPLAETVDAFERALIADALRRYGSLART
CCHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEALLIAKTTLHDKIRKYGLHGE
HHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TDDLKILIIEDDPDVALGCEQALQLEGLAAECAGSAELARHRLHADFPGIVISDIRLPG
CCCEEEEEEECCCCCCCCHHHHHHHCCCHHHHCCHHHHHHHHHCCCCCCEEEEEEECCC
QDGLSLQRELHALDPELPVLLITGHGDVSMAVQAMKDGAYDFLQKPFAPEQLVDIARRAL
CCCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHH
EKRRLVLEVRALRRQLDQRNLLEARLIGNSAGMARVRGLIGALASSAADVLIHGETGTGK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCH
ELVARCLHDASPRAKGNFVAINCGGLPETLFESEIFGHEAGAYTGAGKRRIGKIEYANGG
HHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCC
TLFLDEIETMPVPMQIKLLRVLQERTLERLGSNTAIPIDCRIIAATKEDLTALSEQGKFR
EEEEECHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECHHHHHHHHHCCCEE
ADLYYRLSVANLPLPPLRERREDIPLLFEHFLLQAAARHERAVPTTSAGRMQQLVGYHWP
EEEEEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCC
GNVRELRNVADRCVLGIEAGSPPFGQPAGDAPRPLAETVDAFERALIADALRRYGSLART
CCHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEALLIAKTTLHDKIRKYGLHGE
HHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]