Click here to switch to the map view.

The map label for this gene is nac [H]

Identifier: 71906116

GI number: 71906116

Start: 536292

End: 537251

Strand: Reverse

Name: nac [H]

Synonym: Daro_0476

Alternate gene names: 71906116

Gene position: 537251-536292 (Counterclockwise)

Preceding gene: 71906117

Following gene: 71906115

Centisome position: 11.94

GC content: 67.71

Gene sequence:

>960_bases
ATGGAACTGCGCCAGCTCCGCTACTTTCTCGCCATCGCCGAACACGGCAGCTTCTCCAAAGCTGCCAGCTCGGTCCATGT
CGCACAATCCGCGCTCAGCCACCAACTCGCGCAACTGGAAGACGAACTCGGCCAGCCGCTCTTTCACCGCCTGCCGCGCG
GGGTGGCGCTGACACCGGCCGGTCGGGCCTTCCATCCGCATGCCCTGTCCATCCTGCGCCAGGTCGAGGATGCCCGGCAC
AGCGTCACCCAGGCCGAGGGCGAGACCGTCGGCAAGGTCATTTTCGGCATCCCGCATAGCGTTTCACAAGCGCTCGCCCT
GCCGTTGCTCAAGGCCGTACGTGCCGCCCTGCCCCGCGTCGAGCTGGAACTGACCGAAGAACTGACCGGCAACCTGATCC
CGCAGTTGCGGACCGGCCTGATCCACCTGGCGGTTCTTTTCGACGACGGCCAACTCGACGAATTCCGCTGCCACCCGCTG
CTCGAAGAAGCGCTGCTGCTGATCTCCCCCGCCGAAGCACCCGACGCGCCAAGCGGCCCGATCAGCCTGCGCGACGCCCT
GCGCCTGCCGCTGATCCTGCCCGCATGGCCCCACGGCGTCCGCCCCATCGTCGAGGCGGCCGCCAGACAAGCCGGCCGCG
CTGCCCCCAACGTGGTTGCCGACATCAGCTCGATCAGCATCCTGCGCACCAGCCTGCTCGCCGGCCTCGGTCACACCCTG
CTACCACCGATGCCACTGCTGCACGAACTGGACAGCGGCGCCCTGCGGGCCACCCCCATCGCACCGGAAACCCTGACCCG
CCGCATCATGCTCTGCGCCTCCCGCCAGATTCCCGCCTCCGCCGCCACCCTGGCCGTCGCCCATGTGGCGCAGACATTGG
TCGGCCAGCTCTGCGCCGATGGCAGCTGGCGTGAAGCCCAACTGATCGCGACCGAATCGCCGCCTTTTATTGAGAGTTAA

Upstream 100 bases:

>100_bases
GCCATTAGAACGCAGACGAATCCATCTGAAAAATTCAGAATTACGATATAGCCATCTTGAAAAACGATAGACTGACTGTT
CTGCATCTATCCGTACCGAC

Downstream 100 bases:

>100_bases
ACGGGCTCGGGCGTTGCTGGGGAAAATTGATAGCTGCCTTCGGCCACAACCAGCCATGAACGATTTGTCCAGATAGCGGA
CATCGAAGCGTCCGTTTTAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MELRQLRYFLAIAEHGSFSKAASSVHVAQSALSHQLAQLEDELGQPLFHRLPRGVALTPAGRAFHPHALSILRQVEDARH
SVTQAEGETVGKVIFGIPHSVSQALALPLLKAVRAALPRVELELTEELTGNLIPQLRTGLIHLAVLFDDGQLDEFRCHPL
LEEALLLISPAEAPDAPSGPISLRDALRLPLILPAWPHGVRPIVEAAARQAGRAAPNVVADISSISILRTSLLAGLGHTL
LPPMPLLHELDSGALRATPIAPETLTRRIMLCASRQIPASAATLAVAHVAQTLVGQLCADGSWREAQLIATESPPFIES

Sequences:

>Translated_319_residues
MELRQLRYFLAIAEHGSFSKAASSVHVAQSALSHQLAQLEDELGQPLFHRLPRGVALTPAGRAFHPHALSILRQVEDARH
SVTQAEGETVGKVIFGIPHSVSQALALPLLKAVRAALPRVELELTEELTGNLIPQLRTGLIHLAVLFDDGQLDEFRCHPL
LEEALLLISPAEAPDAPSGPISLRDALRLPLILPAWPHGVRPIVEAAARQAGRAAPNVVADISSISILRTSLLAGLGHTL
LPPMPLLHELDSGALRATPIAPETLTRRIMLCASRQIPASAATLAVAHVAQTLVGQLCADGSWREAQLIATESPPFIES
>Mature_319_residues
MELRQLRYFLAIAEHGSFSKAASSVHVAQSALSHQLAQLEDELGQPLFHRLPRGVALTPAGRAFHPHALSILRQVEDARH
SVTQAEGETVGKVIFGIPHSVSQALALPLLKAVRAALPRVELELTEELTGNLIPQLRTGLIHLAVLFDDGQLDEFRCHPL
LEEALLLISPAEAPDAPSGPISLRDALRLPLILPAWPHGVRPIVEAAARQAGRAAPNVVADISSISILRTSLLAGLGHTL
LPPMPLLHELDSGALRATPIAPETLTRRIMLCASRQIPASAATLAVAHVAQTLVGQLCADGSWREAQLIATESPPFIES

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=243, Percent_Identity=33.3333333333333, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI157672245, Length=293, Percent_Identity=31.0580204778157, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788887, Length=263, Percent_Identity=30.7984790874525, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1790399, Length=243, Percent_Identity=32.0987654320988, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787601, Length=232, Percent_Identity=28.448275862069, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1788748, Length=293, Percent_Identity=24.9146757679181, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1790208, Length=273, Percent_Identity=29.3040293040293, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1789506, Length=230, Percent_Identity=23.4782608695652, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787879, Length=281, Percent_Identity=25.9786476868327, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787806, Length=269, Percent_Identity=27.5092936802974, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87082132, Length=270, Percent_Identity=25.1851851851852, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI145693105, Length=251, Percent_Identity=23.9043824701195, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787949, Length=135, Percent_Identity=29.6296296296296, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1788706, Length=178, Percent_Identity=31.4606741573034, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI145693193, Length=124, Percent_Identity=30.6451612903226, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788481, Length=205, Percent_Identity=24.8780487804878, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI1786401, Length=201, Percent_Identity=26.865671641791, Blast_Score=61, Evalue=7e-11,
Organism=Escherichia coli, GI1790262, Length=210, Percent_Identity=27.6190476190476, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34133; Mature: 34133

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRQLRYFLAIAEHGSFSKAASSVHVAQSALSHQLAQLEDELGQPLFHRLPRGVALTPA
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEECCC
GRAFHPHALSILRQVEDARHSVTQAEGETVGKVIFGIPHSVSQALALPLLKAVRAALPRV
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCH
ELELTEELTGNLIPQLRTGLIHLAVLFDDGQLDEFRCHPLLEEALLLISPAEAPDAPSGP
HHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCHHCCHHHHHHHHHCCCCCCCCCCCCC
ISLRDALRLPLILPAWPHGVRPIVEAAARQAGRAAPNVVADISSISILRTSLLAGLGHTL
CCHHHHHCCCEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHH
LPPMPLLHELDSGALRATPIAPETLTRRIMLCASRQIPASAATLAVAHVAQTLVGQLCAD
CCCCHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
GSWREAQLIATESPPFIES
CCCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MELRQLRYFLAIAEHGSFSKAASSVHVAQSALSHQLAQLEDELGQPLFHRLPRGVALTPA
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEECCC
GRAFHPHALSILRQVEDARHSVTQAEGETVGKVIFGIPHSVSQALALPLLKAVRAALPRV
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCH
ELELTEELTGNLIPQLRTGLIHLAVLFDDGQLDEFRCHPLLEEALLLISPAEAPDAPSGP
HHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCHHCCHHHHHHHHHCCCCCCCCCCCCC
ISLRDALRLPLILPAWPHGVRPIVEAAARQAGRAAPNVVADISSISILRTSLLAGLGHTL
CCHHHHHCCCEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHH
LPPMPLLHELDSGALRATPIAPETLTRRIMLCASRQIPASAATLAVAHVAQTLVGQLCAD
CCCCHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
GSWREAQLIATESPPFIES
CCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8458853 [H]