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The map label for this gene is aceE [H]

Identifier: 71906082

GI number: 71906082

Start: 485768

End: 488443

Strand: Reverse

Name: aceE [H]

Synonym: Daro_0442

Alternate gene names: 71906082

Gene position: 488443-485768 (Counterclockwise)

Preceding gene: 71906084

Following gene: 71906081

Centisome position: 10.85

GC content: 61.21

Gene sequence:

>2676_bases
ATGGCCGAACAACCGAATGCCTTGCCTGACCCCGCTGACGTAGATCCGCAGGAAACAAAAGAATGGATGGACGCCCTCGA
CGGCGTGATCAACCAGGAAGGTCCCACCCGCGCCCATTACCTGATTGAAAAGGTGATCGGCCAAGCCCGTCTGCAGGGCG
TCGACATCCCCTACTCGGCCAATACCGAATACATCAACACCATTCCGGCCCACCAGCAGCCCAAGTACCCGGGCAACGCC
GACATGGAAATCAAGATTCACTCCTACATTCGCTGGAACGCCATGGCCATGGTCGTGCGTGCCAACAAGGACACCAACGT
CGGCGGCCACATTGCCTCCTTCGCTTCAGCCGCCGCCTTGTACGACGTCGGCTTTTCGCATTTCTGGCGCAGCATCAATG
ATCCGTCCGGCGGCGACCTGATCTTCTTCCAGGGCCACTCGGTGCCGGGCGTTTATTCCCGCGCCTTCATGCTCGGCCGC
CTGACCGCCGAGCAGATGGACAACTTCCGCCAGGAAACCGACGGCAAGGGCATTTCGTCCTATCCGCACCCGTGGCTGAT
GCCGGACTTCTGGCAGTTCCCGACCGTTTCCATGGGCCTCGGCCCGATCCAGGCCATCTACCAGGCCCGCTTCATGAAGT
ATCTCGACTCGCGCGGACTGGCCAAGGCCGGTGATCGCAAGGTCTGGGCCTTCCTCGGTGACGGCGAGACCGACGAAGTC
GAATCGCTCGGCGCCATCGGCATGGCCGGTCGCGAGAAGCTCGACAACCTGATTTTCGTGATCAACTGCAATCTGCAGCG
CCTGGACGGTCCGGTGCGCGGCAACGGCAAGATCATCCAGGAACTCGAATCCGAGTTCCGCGGTTCCGGCTGGAACGTCA
TCAAGCTGATCTGGGGCACGCAGTGGGACACGCTGTTCCAGCGCGACAAGAAGGGCATCATGAAGCAGCGCATGATGGAA
CTGTGCGACGGCGAGTATCAGACCTTCAAGGCCAAGAACGGCGCCTACGTTCGCGAACACTTCTTCAACACGCCGGAACT
CAAGGAACTGGTCGCTGACTGGACCGATGACCAGGTTTGGCAACTGAACCGCGGCGGCCACGACATCTTCAAGATTTTTG
CCGCCTACAACGCCGCCGTCACCCACAAGGGCCAGCCGACGCTGATCCTGGCCAAGACCATCAAGGGTTTCGGCATGGGC
CAGGCCGGCGAAGCGATGAACATTTCCCACCAGCAGAAGAAGATGGACATCGAGCAGATCGGCCGCTTCCGCGACCGTTT
CGGCCTGCCAGTGCCGGACGACAAGCTCTACGAACTGCCCTACCTGAAGTTCGCCGAAGACTCCGAGGAATACAAGTACA
TGCTTCAGCGCCGCATGGACCTCGGCGGCTTCCTGCCGCAGCGCCGCCGCAAGGCCGAGCCGCTGCAGATTCCGGCCCTC
GACACCTTTGCCGCTCTGTTGAAGGCATCCGGCGAAGGCCGCGAGTTGTCCACGACGATGGCCATCGTCCGCATCATGAA
CATGATGTTGAAGGACAAGAATGTCGGCAAGAACGTCGTGCCCATCGTGCCGGACGAGTCCCGCACCTTCGGCATGGAAG
GCATGTTCCGCTCGGTCGGCATCTGGAACCAGGAAGGCCAGAACTATGTGCCGGAAGACCACGACCAGCTGATGTTCTAC
AAGGAGTCGAAAACCGGCCAGGTACTGCAGGAAGGCATCAACGAATCCGGCGCCATGAGCGACTGGATCGCCGCCGCCAC
CTCGTACTCCGTGCATAACGTGCAGACCATTCCGTTCTACATCTGCTACTCGATGTTCGGCATGCAGCGCGTCCTCGACC
TCTGCTGGGCTGCCGGCGACCAACGCGCCCGCGGCTTCCTGATCGGCGGTACCGCCGGTCGTACGACACTGAACGGCGAA
GGCCTGCAGCACGAAGACGGCCACAGCCTGATCCTGTCCAACCTGATCCCGAACTGCGTTTCCTACGACCCGACCTTCCA
GTATGAAGTCGCCGTCGTGGCCCAGGACGGCATGCGCCGCATGTTCCAGGAGCAGGAAGACGTCTTCTATTACCTGACGG
TAATGAACGAGAACTACGAGCACCCGGAAATGCCGGTCGGCGCCGAAGCCGACATCATCAAGGGCATGTACCTGTTCAAG
AAGGGCGCCGATTCGGCTGCCCCGCGCGTTCAGCTGCTCGGCTCCGGCACCATCTTCCGCGAAGTCATCGCCGCTGCCGA
CCTGCTCAAGCAGGACTGGGGTGTCGAGGCCGACCTGTGGGGCTGTACCAGCATGAACGAACTGGCCCGCAACGGTCAAG
ACGTCCAGCGCTGGAACATGCTGCATCCGCTGGAAGCGCCGAAGCTGTCGCACGTCGAAGAGAAGCTGGCTGGCGCCAAG
GGCCCGGTCATCGCCTCGACCGACTACATCAAGCTGTTCTCCGAGCAGATCCGCCCCTTCGTCAAGGCACCGTACGTCAC
GCTGGGCACCGATGGTTTCGGCCGTTCGGATACCCGCGAGAAGCTGCGTCACCATTTCGAGGTTGATCGTCACTGGGTCA
CGCTGGCTGCCCTCAAGGCACTGGCCGACAACGGTGAAGTCAAGCGCGAAGTCGTCGCCGCCGCTCTGGTCAAGTACAAC
CTTGACCCGAACAAGCCGAATCCGCTGAGCGTTTAA

Upstream 100 bases:

>100_bases
TGATTAGGGAAAACACGGGCTCGCGAACCGACTCGTAACGAGCTAAAGTTGTTTTGCTCAACAGGCAAAACAGAATGAAA
TGCAACCTAGGAGACAAACC

Downstream 100 bases:

>100_bases
GGGAGAGACAACATGAGCCAAATCATTGAAGTCACAGTCCCCGATATCGGCGATTTCGACAGCGTTCCGGTCATCGACCT
GTTCGTCAAAGTCGGCGACA

Product: pyruvate dehydrogenase subunit E1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 891; Mature: 890

Protein sequence:

>891_residues
MAEQPNALPDPADVDPQETKEWMDALDGVINQEGPTRAHYLIEKVIGQARLQGVDIPYSANTEYINTIPAHQQPKYPGNA
DMEIKIHSYIRWNAMAMVVRANKDTNVGGHIASFASAAALYDVGFSHFWRSINDPSGGDLIFFQGHSVPGVYSRAFMLGR
LTAEQMDNFRQETDGKGISSYPHPWLMPDFWQFPTVSMGLGPIQAIYQARFMKYLDSRGLAKAGDRKVWAFLGDGETDEV
ESLGAIGMAGREKLDNLIFVINCNLQRLDGPVRGNGKIIQELESEFRGSGWNVIKLIWGTQWDTLFQRDKKGIMKQRMME
LCDGEYQTFKAKNGAYVREHFFNTPELKELVADWTDDQVWQLNRGGHDIFKIFAAYNAAVTHKGQPTLILAKTIKGFGMG
QAGEAMNISHQQKKMDIEQIGRFRDRFGLPVPDDKLYELPYLKFAEDSEEYKYMLQRRMDLGGFLPQRRRKAEPLQIPAL
DTFAALLKASGEGRELSTTMAIVRIMNMMLKDKNVGKNVVPIVPDESRTFGMEGMFRSVGIWNQEGQNYVPEDHDQLMFY
KESKTGQVLQEGINESGAMSDWIAAATSYSVHNVQTIPFYICYSMFGMQRVLDLCWAAGDQRARGFLIGGTAGRTTLNGE
GLQHEDGHSLILSNLIPNCVSYDPTFQYEVAVVAQDGMRRMFQEQEDVFYYLTVMNENYEHPEMPVGAEADIIKGMYLFK
KGADSAAPRVQLLGSGTIFREVIAAADLLKQDWGVEADLWGCTSMNELARNGQDVQRWNMLHPLEAPKLSHVEEKLAGAK
GPVIASTDYIKLFSEQIRPFVKAPYVTLGTDGFGRSDTREKLRHHFEVDRHWVTLAALKALADNGEVKREVVAAALVKYN
LDPNKPNPLSV

Sequences:

>Translated_891_residues
MAEQPNALPDPADVDPQETKEWMDALDGVINQEGPTRAHYLIEKVIGQARLQGVDIPYSANTEYINTIPAHQQPKYPGNA
DMEIKIHSYIRWNAMAMVVRANKDTNVGGHIASFASAAALYDVGFSHFWRSINDPSGGDLIFFQGHSVPGVYSRAFMLGR
LTAEQMDNFRQETDGKGISSYPHPWLMPDFWQFPTVSMGLGPIQAIYQARFMKYLDSRGLAKAGDRKVWAFLGDGETDEV
ESLGAIGMAGREKLDNLIFVINCNLQRLDGPVRGNGKIIQELESEFRGSGWNVIKLIWGTQWDTLFQRDKKGIMKQRMME
LCDGEYQTFKAKNGAYVREHFFNTPELKELVADWTDDQVWQLNRGGHDIFKIFAAYNAAVTHKGQPTLILAKTIKGFGMG
QAGEAMNISHQQKKMDIEQIGRFRDRFGLPVPDDKLYELPYLKFAEDSEEYKYMLQRRMDLGGFLPQRRRKAEPLQIPAL
DTFAALLKASGEGRELSTTMAIVRIMNMMLKDKNVGKNVVPIVPDESRTFGMEGMFRSVGIWNQEGQNYVPEDHDQLMFY
KESKTGQVLQEGINESGAMSDWIAAATSYSVHNVQTIPFYICYSMFGMQRVLDLCWAAGDQRARGFLIGGTAGRTTLNGE
GLQHEDGHSLILSNLIPNCVSYDPTFQYEVAVVAQDGMRRMFQEQEDVFYYLTVMNENYEHPEMPVGAEADIIKGMYLFK
KGADSAAPRVQLLGSGTIFREVIAAADLLKQDWGVEADLWGCTSMNELARNGQDVQRWNMLHPLEAPKLSHVEEKLAGAK
GPVIASTDYIKLFSEQIRPFVKAPYVTLGTDGFGRSDTREKLRHHFEVDRHWVTLAALKALADNGEVKREVVAAALVKYN
LDPNKPNPLSV
>Mature_890_residues
AEQPNALPDPADVDPQETKEWMDALDGVINQEGPTRAHYLIEKVIGQARLQGVDIPYSANTEYINTIPAHQQPKYPGNAD
MEIKIHSYIRWNAMAMVVRANKDTNVGGHIASFASAAALYDVGFSHFWRSINDPSGGDLIFFQGHSVPGVYSRAFMLGRL
TAEQMDNFRQETDGKGISSYPHPWLMPDFWQFPTVSMGLGPIQAIYQARFMKYLDSRGLAKAGDRKVWAFLGDGETDEVE
SLGAIGMAGREKLDNLIFVINCNLQRLDGPVRGNGKIIQELESEFRGSGWNVIKLIWGTQWDTLFQRDKKGIMKQRMMEL
CDGEYQTFKAKNGAYVREHFFNTPELKELVADWTDDQVWQLNRGGHDIFKIFAAYNAAVTHKGQPTLILAKTIKGFGMGQ
AGEAMNISHQQKKMDIEQIGRFRDRFGLPVPDDKLYELPYLKFAEDSEEYKYMLQRRMDLGGFLPQRRRKAEPLQIPALD
TFAALLKASGEGRELSTTMAIVRIMNMMLKDKNVGKNVVPIVPDESRTFGMEGMFRSVGIWNQEGQNYVPEDHDQLMFYK
ESKTGQVLQEGINESGAMSDWIAAATSYSVHNVQTIPFYICYSMFGMQRVLDLCWAAGDQRARGFLIGGTAGRTTLNGEG
LQHEDGHSLILSNLIPNCVSYDPTFQYEVAVVAQDGMRRMFQEQEDVFYYLTVMNENYEHPEMPVGAEADIIKGMYLFKK
GADSAAPRVQLLGSGTIFREVIAAADLLKQDWGVEADLWGCTSMNELARNGQDVQRWNMLHPLEAPKLSHVEEKLAGAKG
PVIASTDYIKLFSEQIRPFVKAPYVTLGTDGFGRSDTREKLRHHFEVDRHWVTLAALKALADNGEVKREVVAAALVKYNL
DPNKPNPLSV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG2609

COG function: function code C; Pyruvate dehydrogenase complex, dehydrogenase (E1) component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786304, Length=884, Percent_Identity=58.4841628959276, Blast_Score=1084, Evalue=0.0,

Paralogues:

None

Copy number: 1140 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 400 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004660
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005474 [H]

Pfam domain/function: PF00456 Transketolase_N [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 100251; Mature: 100120

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQPNALPDPADVDPQETKEWMDALDGVINQEGPTRAHYLIEKVIGQARLQGVDIPYSA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
NTEYINTIPAHQQPKYPGNADMEIKIHSYIRWNAMAMVVRANKDTNVGGHIASFASAAAL
CCCCEECCCCCCCCCCCCCCCEEEEEECEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHH
YDVGFSHFWRSINDPSGGDLIFFQGHSVPGVYSRAFMLGRLTAEQMDNFRQETDGKGISS
HHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
YPHPWLMPDFWQFPTVSMGLGPIQAIYQARFMKYLDSRGLAKAGDRKVWAFLGDGETDEV
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHH
ESLGAIGMAGREKLDNLIFVINCNLQRLDGPVRGNGKIIQELESEFRGSGWNVIKLIWGT
HHHHHHCCCCHHHHCCEEEEEECCHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECC
QWDTLFQRDKKGIMKQRMMELCDGEYQTFKAKNGAYVREHFFNTPELKELVADWTDDQVW
CHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHCCCCHHHHHHHHCCCCCCEE
QLNRGGHDIFKIFAAYNAAVTHKGQPTLILAKTIKGFGMGQAGEAMNISHQQKKMDIEQI
EECCCCHHHHHHHHHHHHHEECCCCCCEEEEEHHCCCCCCCCCCCCCCCHHHHHCCHHHH
GRFRDRFGLPVPDDKLYELPYLKFAEDSEEYKYMLQRRMDLGGFLPQRRRKAEPLQIPAL
HHHHHHCCCCCCCCCCEECCCEEECCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCH
DTFAALLKASGEGRELSTTMAIVRIMNMMLKDKNVGKNVVPIVPDESRTFGMEGMFRSVG
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHHHHHCC
IWNQEGQNYVPEDHDQLMFYKESKTGQVLQEGINESGAMSDWIAAATSYSVHNVQTIPFY
CCCCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEHHHHH
ICYSMFGMQRVLDLCWAAGDQRARGFLIGGTAGRTTLNGEGLQHEDGHSLILSNLIPNCV
HHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEECCCCCCCCCCCHHHHHHHCCHHH
SYDPTFQYEVAVVAQDGMRRMFQEQEDVFYYLTVMNENYEHPEMPVGAEADIIKGMYLFK
CCCCCCEEEEEEEEHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHH
KGADSAAPRVQLLGSGTIFREVIAAADLLKQDWGVEADLWGCTSMNELARNGQDVQRWNM
CCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCHHHHHCC
LHPLEAPKLSHVEEKLAGAKGPVIASTDYIKLFSEQIRPFVKAPYVTLGTDGFGRSDTRE
CCCCCCCCHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHH
KLRHHFEVDRHWVTLAALKALADNGEVKREVVAAALVKYNLDPNKPNPLSV
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AEQPNALPDPADVDPQETKEWMDALDGVINQEGPTRAHYLIEKVIGQARLQGVDIPYSA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
NTEYINTIPAHQQPKYPGNADMEIKIHSYIRWNAMAMVVRANKDTNVGGHIASFASAAAL
CCCCEECCCCCCCCCCCCCCCEEEEEECEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHH
YDVGFSHFWRSINDPSGGDLIFFQGHSVPGVYSRAFMLGRLTAEQMDNFRQETDGKGISS
HHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
YPHPWLMPDFWQFPTVSMGLGPIQAIYQARFMKYLDSRGLAKAGDRKVWAFLGDGETDEV
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHH
ESLGAIGMAGREKLDNLIFVINCNLQRLDGPVRGNGKIIQELESEFRGSGWNVIKLIWGT
HHHHHHCCCCHHHHCCEEEEEECCHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECC
QWDTLFQRDKKGIMKQRMMELCDGEYQTFKAKNGAYVREHFFNTPELKELVADWTDDQVW
CHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHCCCCHHHHHHHHCCCCCCEE
QLNRGGHDIFKIFAAYNAAVTHKGQPTLILAKTIKGFGMGQAGEAMNISHQQKKMDIEQI
EECCCCHHHHHHHHHHHHHEECCCCCCEEEEEHHCCCCCCCCCCCCCCCHHHHHCCHHHH
GRFRDRFGLPVPDDKLYELPYLKFAEDSEEYKYMLQRRMDLGGFLPQRRRKAEPLQIPAL
HHHHHHCCCCCCCCCCEECCCEEECCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCH
DTFAALLKASGEGRELSTTMAIVRIMNMMLKDKNVGKNVVPIVPDESRTFGMEGMFRSVG
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHHHHHCC
IWNQEGQNYVPEDHDQLMFYKESKTGQVLQEGINESGAMSDWIAAATSYSVHNVQTIPFY
CCCCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEHHHHH
ICYSMFGMQRVLDLCWAAGDQRARGFLIGGTAGRTTLNGEGLQHEDGHSLILSNLIPNCV
HHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEECCCCCCCCCCCHHHHHHHCCHHH
SYDPTFQYEVAVVAQDGMRRMFQEQEDVFYYLTVMNENYEHPEMPVGAEADIIKGMYLFK
CCCCCCEEEEEEEEHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHH
KGADSAAPRVQLLGSGTIFREVIAAADLLKQDWGVEADLWGCTSMNELARNGQDVQRWNM
CCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCHHHHHCC
LHPLEAPKLSHVEEKLAGAKGPVIASTDYIKLFSEQIRPFVKAPYVTLGTDGFGRSDTRE
CCCCCCCCHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHH
KLRHHFEVDRHWVTLAALKALADNGEVKREVVAAALVKYNLDPNKPNPLSV
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8021225 [H]