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The map label for this gene is pdhB [H]

Identifier: 71906081

GI number: 71906081

Start: 484115

End: 485755

Strand: Reverse

Name: pdhB [H]

Synonym: Daro_0441

Alternate gene names: 71906081

Gene position: 485755-484115 (Counterclockwise)

Preceding gene: 71906082

Following gene: 71906080

Centisome position: 10.79

GC content: 62.64

Gene sequence:

>1641_bases
ATGAGCCAAATCATTGAAGTCACAGTCCCCGATATCGGCGATTTCGACAGCGTTCCGGTCATCGACCTGTTCGTCAAAGT
CGGCGACACCATCAAGGTTGACGACGCCATCGTCACGCTGGAATCCGACAAGGCCACGATGGATGTTCCTTCCACCGTTG
CCGGCGTCGTCAAGGAAGTGCTGGTCTCGCTCGGCTCCAAAGTCGGCGAAGGCGCCCTGCTGATCAAGGTTGAAACCGGC
GGCGCTGCGGCCGAGCCGGCAGCCGCCCCTGCTCCGGCGGCGCAAGCCGCTGCGCCGGCACCAGCACCCGCTGCTGCACC
GGTCGCTGCTGCGCCTGCAGCCGGTGGTGGCGTGGTTGAAGTCAAGGTCCCGGATATTGGCGATTTCGCCGAAGTCCCGG
TCATCGAGTTGTACGTCAAGGTTGGCGACGCCATCAAGGTTGATGACGCGATCGCCACGCTGGAATCCGACAAGGCGACG
ATGGATGTTCCGTCCACCGTCGCCGGTACGGTCAAGGAAGTGCTCGTCCAGATCGGCTCCAAGGTCGGTGAAGGCACGGT
TCTGATCAAGGTTGAAAGTGGTGCCTCGGCTCCGGCCGCTGCCCCGCAAGCTGCTGCTCCGGCACCTGCTGCTGCAGCTC
CGGCTCCCGTTGCCGCGCCTGTTGCTGCTCCGGCAGCCGCATCCCCGGCCGCACTAGCCCCGGCACTGGCCGCTGGCAGC
AAGGTTCACGCCTCGCCGTCGGTCCGTGCCTATGCCCGCGAACTCGGTGTCGATCTGAACAAGGTGCCGGCGACCGGCCC
GAAAAACCGCATCGTCAAGGAAGACCTGACCAAGTACGTCAAGGGCGTCATGTCCGGTGCTGTAGCCGGACCGACTGCTG
GCGGCGGTGCCAGCCTCGGCGGCGGTCTCGATCTGCTGCCCTGGCCGAAGGTTGATTTCGCCAAGTTCGGCGAAATCGAG
GTCAAGCCGCTGTCGCGCATCAAGAAGATTTCCGGCCAGAACCTGTCGCGCAACTGGGTGATGATCCCGGCCGTGACCTA
TCACGAAGATGCCGACATCACCGATATCGAAGCCTTCCGCGTGCTGCTCAACAAGGAAAACGAAAAGGGTGGCGGCGCCA
AGCTGACCATGCTCGCTTTCCTGATGAAGGCTTGCGTCAAGGGCCTGCAGAAATTCCCGGAATTCAATTCCTCGCTCGAC
GGCGACAATCTGGTGCTGAAGAAGTATTTCAACATCGGTTTCGCGGCTGATACCCCGAACGGCCTGGTCGTGCCGGTGGT
CAAGAATGTCGACAAGAAGTCGGTCTTCGAACTGGCGCAGGAATCCGGTGATCTGGCCAAGCAGGCGCGTGACGGCAAGC
TGAAGCCGGCCGACATGTCCGGTGCCTGCTTCACGATTTCCAGCCTGGGCGGTATCGGCGGCACTTACTTCGCACCGATC
GTCAATGCACCGGAAGTCGCCATCCTCGGCGTCAACAAGTCGGCCATGAAACCGGTCTGGGACGGCAAGCAATTCGTGCC
GCGCCTCGTCCTGCCGCTGTCGCTGACCGCCGACCACCGCGTCATCGACGGTGCGCTGGCGACCCGCTTCAATGTCTATA
TCGCCCAGCTGCTGGCCGACTTCCGTCGCGTCGCGCTGTAA

Upstream 100 bases:

>100_bases
CAACGGTGAAGTCAAGCGCGAAGTCGTCGCCGCCGCTCTGGTCAAGTACAACCTTGACCCGAACAAGCCGAATCCGCTGA
GCGTTTAAGGGAGAGACAAC

Downstream 100 bases:

>100_bases
AGGGGAATGACCATGAGCAATCTGGTAGAAGTCAAAGTCCCCGATATCGGCGATTTCGCCGAAGTGCCGATCATCGACCT
GTTCGTCAAGGTCGGCGACA

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MSQIIEVTVPDIGDFDSVPVIDLFVKVGDTIKVDDAIVTLESDKATMDVPSTVAGVVKEVLVSLGSKVGEGALLIKVETG
GAAAEPAAAPAPAAQAAAPAPAPAAAPVAAAPAAGGGVVEVKVPDIGDFAEVPVIELYVKVGDAIKVDDAIATLESDKAT
MDVPSTVAGTVKEVLVQIGSKVGEGTVLIKVESGASAPAAAPQAAAPAPAAAAPAPVAAPVAAPAAASPAALAPALAAGS
KVHASPSVRAYARELGVDLNKVPATGPKNRIVKEDLTKYVKGVMSGAVAGPTAGGGASLGGGLDLLPWPKVDFAKFGEIE
VKPLSRIKKISGQNLSRNWVMIPAVTYHEDADITDIEAFRVLLNKENEKGGGAKLTMLAFLMKACVKGLQKFPEFNSSLD
GDNLVLKKYFNIGFAADTPNGLVVPVVKNVDKKSVFELAQESGDLAKQARDGKLKPADMSGACFTISSLGGIGGTYFAPI
VNAPEVAILGVNKSAMKPVWDGKQFVPRLVLPLSLTADHRVIDGALATRFNVYIAQLLADFRRVAL

Sequences:

>Translated_546_residues
MSQIIEVTVPDIGDFDSVPVIDLFVKVGDTIKVDDAIVTLESDKATMDVPSTVAGVVKEVLVSLGSKVGEGALLIKVETG
GAAAEPAAAPAPAAQAAAPAPAPAAAPVAAAPAAGGGVVEVKVPDIGDFAEVPVIELYVKVGDAIKVDDAIATLESDKAT
MDVPSTVAGTVKEVLVQIGSKVGEGTVLIKVESGASAPAAAPQAAAPAPAAAAPAPVAAPVAAPAAASPAALAPALAAGS
KVHASPSVRAYARELGVDLNKVPATGPKNRIVKEDLTKYVKGVMSGAVAGPTAGGGASLGGGLDLLPWPKVDFAKFGEIE
VKPLSRIKKISGQNLSRNWVMIPAVTYHEDADITDIEAFRVLLNKENEKGGGAKLTMLAFLMKACVKGLQKFPEFNSSLD
GDNLVLKKYFNIGFAADTPNGLVVPVVKNVDKKSVFELAQESGDLAKQARDGKLKPADMSGACFTISSLGGIGGTYFAPI
VNAPEVAILGVNKSAMKPVWDGKQFVPRLVLPLSLTADHRVIDGALATRFNVYIAQLLADFRRVAL
>Mature_545_residues
SQIIEVTVPDIGDFDSVPVIDLFVKVGDTIKVDDAIVTLESDKATMDVPSTVAGVVKEVLVSLGSKVGEGALLIKVETGG
AAAEPAAAPAPAAQAAAPAPAPAAAPVAAAPAAGGGVVEVKVPDIGDFAEVPVIELYVKVGDAIKVDDAIATLESDKATM
DVPSTVAGTVKEVLVQIGSKVGEGTVLIKVESGASAPAAAPQAAAPAPAAAAPAPVAAPVAAPAAASPAALAPALAAGSK
VHASPSVRAYARELGVDLNKVPATGPKNRIVKEDLTKYVKGVMSGAVAGPTAGGGASLGGGLDLLPWPKVDFAKFGEIEV
KPLSRIKKISGQNLSRNWVMIPAVTYHEDADITDIEAFRVLLNKENEKGGGAKLTMLAFLMKACVKGLQKFPEFNSSLDG
DNLVLKKYFNIGFAADTPNGLVVPVVKNVDKKSVFELAQESGDLAKQARDGKLKPADMSGACFTISSLGGIGGTYFAPIV
NAPEVAILGVNKSAMKPVWDGKQFVPRLVLPLSLTADHRVIDGALATRFNVYIAQLLADFRRVAL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=450, Percent_Identity=30.2222222222222, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI31711992, Length=552, Percent_Identity=30.2536231884058, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI203098816, Length=501, Percent_Identity=25.3493013972056, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI203098753, Length=444, Percent_Identity=25.6756756756757, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI19923748, Length=209, Percent_Identity=31.5789473684211, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI260898739, Length=147, Percent_Identity=33.3333333333333, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI1786305, Length=545, Percent_Identity=50.8256880733945, Blast_Score=455, Evalue=1e-129,
Organism=Escherichia coli, GI1786946, Length=432, Percent_Identity=28.7037037037037, Blast_Score=165, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=421, Percent_Identity=31.8289786223278, Blast_Score=190, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17560088, Length=431, Percent_Identity=28.3062645011601, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI25146366, Length=411, Percent_Identity=30.6569343065693, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17538894, Length=323, Percent_Identity=28.7925696594427, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6320352, Length=427, Percent_Identity=29.7423887587822, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6324258, Length=422, Percent_Identity=26.7772511848341, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI18859875, Length=439, Percent_Identity=31.2072892938497, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24582497, Length=223, Percent_Identity=30.9417040358744, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI20129315, Length=223, Percent_Identity=30.9417040358744, Blast_Score=112, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24645909, Length=220, Percent_Identity=31.8181818181818, Blast_Score=101, Evalue=2e-21,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 55741; Mature: 55610

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQIIEVTVPDIGDFDSVPVIDLFVKVGDTIKVDDAIVTLESDKATMDVPSTVAGVVKEV
CCCEEEEECCCCCCCCCCCHHHHHHHCCCEEEECCEEEEEECCCCCCCCCHHHHHHHHHH
LVSLGSKVGEGALLIKVETGGAAAEPAAAPAPAAQAAAPAPAPAAAPVAAAPAAGGGVVE
HHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCEEE
VKVPDIGDFAEVPVIELYVKVGDAIKVDDAIATLESDKATMDVPSTVAGTVKEVLVQIGS
EECCCCCCCCCCCCEEEEEECCCCEEECHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHC
KVGEGTVLIKVESGASAPAAAPQAAAPAPAAAAPAPVAAPVAAPAAASPAALAPALAAGS
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCC
KVHASPSVRAYARELGVDLNKVPATGPKNRIVKEDLTKYVKGVMSGAVAGPTAGGGASLG
EECCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GGLDLLPWPKVDFAKFGEIEVKPLSRIKKISGQNLSRNWVMIPAVTYHEDADITDIEAFR
CCCCCCCCCCCCHHHCCCEEEHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCHHHHHH
VLLNKENEKGGGAKLTMLAFLMKACVKGLQKFPEFNSSLDGDNLVLKKYFNIGFAADTPN
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHCCEEECCCCC
GLVVPVVKNVDKKSVFELAQESGDLAKQARDGKLKPADMSGACFTISSLGGIGGTYFAPI
CEEEHHCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCEEEEHHCCCCCCHHHHHC
VNAPEVAILGVNKSAMKPVWDGKQFVPRLVLPLSLTADHRVIDGALATRFNVYIAQLLAD
CCCCCEEEEECCHHHCCCCCCCHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
FRRVAL
HHHHCC
>Mature Secondary Structure 
SQIIEVTVPDIGDFDSVPVIDLFVKVGDTIKVDDAIVTLESDKATMDVPSTVAGVVKEV
CCEEEEECCCCCCCCCCCHHHHHHHCCCEEEECCEEEEEECCCCCCCCCHHHHHHHHHH
LVSLGSKVGEGALLIKVETGGAAAEPAAAPAPAAQAAAPAPAPAAAPVAAAPAAGGGVVE
HHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCEEE
VKVPDIGDFAEVPVIELYVKVGDAIKVDDAIATLESDKATMDVPSTVAGTVKEVLVQIGS
EECCCCCCCCCCCCEEEEEECCCCEEECHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHC
KVGEGTVLIKVESGASAPAAAPQAAAPAPAAAAPAPVAAPVAAPAAASPAALAPALAAGS
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCC
KVHASPSVRAYARELGVDLNKVPATGPKNRIVKEDLTKYVKGVMSGAVAGPTAGGGASLG
EECCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GGLDLLPWPKVDFAKFGEIEVKPLSRIKKISGQNLSRNWVMIPAVTYHEDADITDIEAFR
CCCCCCCCCCCCHHHCCCEEEHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCHHHHHH
VLLNKENEKGGGAKLTMLAFLMKACVKGLQKFPEFNSSLDGDNLVLKKYFNIGFAADTPN
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHCCEEECCCCC
GLVVPVVKNVDKKSVFELAQESGDLAKQARDGKLKPADMSGACFTISSLGGIGGTYFAPI
CEEEHHCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCEEEEHHCCCCCCHHHHHC
VNAPEVAILGVNKSAMKPVWDGKQFVPRLVLPLSLTADHRVIDGALATRFNVYIAQLLAD
CCCCCEEEEECCHHHCCCCCCCHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
FRRVAL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8021225 [H]