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The map label for this gene is dat [H]

Identifier: 71905932

GI number: 71905932

Start: 331363

End: 332238

Strand: Reverse

Name: dat [H]

Synonym: Daro_0290

Alternate gene names: 71905932

Gene position: 332238-331363 (Counterclockwise)

Preceding gene: 71905933

Following gene: 71905931

Centisome position: 7.38

GC content: 61.87

Gene sequence:

>876_bases
ATGACACCCTACGTCGCCGATCCCGTCTACCTGAATGGCCGTTTCCTGCCGTTGGCGGAAGCGGGTATTTCCCCACTCGA
CCGGGGTTTTCTATACGGCGACGGCGTCTATGAAGTGATCCCGGTCTATTCCCGCCGGGCTTTCCGCATCGACGAACATG
TCAAGCGTCTGCAAGCGACACTGGACGGCATCAAGCTGGCCAACCCGCTGACCGCCGACGGCTGGAAGGAAGTCATTGCC
AAGCTGATCGAAACTGCCCCCTGGGACGACCAGTCGATCTATCTGCAAGTCACCCGCGGCGCCGACGACAAGCGTGACCA
CGCCTTCCCGCCGGCCTCGATACCATCGACAGCCTTTGCCTTCGCTTCGCCGCTGGTCACCACGCCCGCCGAGGTCCGCG
CCAAGGGCGTCGCCGCGATCACCGTGCCCGATCTTCGCTGGTCGCGCTGCGACCTCAAGGTCATCTCGCTGCTCGCCAAC
GTGCTGGCCCGCCAGCAAGCCGTCGATCAAGGCTGTGCCGAGGCACTGTTGATTCGCGACGGTTTCATGAAAGAAGGCGC
TGCCTCCAACATCTTCATCGTCAAGGACGGCGTGCTGGTCGCCCCGCCCAAGACGCACCTGATGCTGCCGGGCATTACCT
ACGACGTGATCCTCGAACTGGCGGCGACCCACCAGCAGGCTTTGGCAGTACGCGAAATCAGCGAAACTGAACTTCGTGGC
GCCGACGAGGTCTGGATGACCTCATCGACCAAGGAAATCCTGGCAATCACGACGCTCGATGGCGAGCCAGTCGGCACCGG
CAAACCCGGCCCGTTCGGCGAGCAGATGTGGCAGTGGTATCAGGATTTCAAGAACACCGTAATGCGTAAAGGCTGA

Upstream 100 bases:

>100_bases
CCGTACAACGAATATCCGGTCGTCGCTCTTGAAAATATCGCACTCGGCAATATCTTCATTCGTATCATCGATACCATCCG
ACTCTGGTTCAACTAAACCC

Downstream 100 bases:

>100_bases
GATGGCTTCGTACAAGGACACCCTCCTCGAATTCCCGTGTGAATTCCCCCTGAAGATCATGGGCAAGGCTGATGACAGCC
TGGCTCAGGTCGTCCTCGAC

Product: branched chain amino acid: 2-keto-4-methylthiobutyrate aminotransferase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTPYVADPVYLNGRFLPLAEAGISPLDRGFLYGDGVYEVIPVYSRRAFRIDEHVKRLQATLDGIKLANPLTADGWKEVIA
KLIETAPWDDQSIYLQVTRGADDKRDHAFPPASIPSTAFAFASPLVTTPAEVRAKGVAAITVPDLRWSRCDLKVISLLAN
VLARQQAVDQGCAEALLIRDGFMKEGAASNIFIVKDGVLVAPPKTHLMLPGITYDVILELAATHQQALAVREISETELRG
ADEVWMTSSTKEILAITTLDGEPVGTGKPGPFGEQMWQWYQDFKNTVMRKG

Sequences:

>Translated_291_residues
MTPYVADPVYLNGRFLPLAEAGISPLDRGFLYGDGVYEVIPVYSRRAFRIDEHVKRLQATLDGIKLANPLTADGWKEVIA
KLIETAPWDDQSIYLQVTRGADDKRDHAFPPASIPSTAFAFASPLVTTPAEVRAKGVAAITVPDLRWSRCDLKVISLLAN
VLARQQAVDQGCAEALLIRDGFMKEGAASNIFIVKDGVLVAPPKTHLMLPGITYDVILELAATHQQALAVREISETELRG
ADEVWMTSSTKEILAITTLDGEPVGTGKPGPFGEQMWQWYQDFKNTVMRKG
>Mature_290_residues
TPYVADPVYLNGRFLPLAEAGISPLDRGFLYGDGVYEVIPVYSRRAFRIDEHVKRLQATLDGIKLANPLTADGWKEVIAK
LIETAPWDDQSIYLQVTRGADDKRDHAFPPASIPSTAFAFASPLVTTPAEVRAKGVAAITVPDLRWSRCDLKVISLLANV
LARQQAVDQGCAEALLIRDGFMKEGAASNIFIVKDGVLVAPPKTHLMLPGITYDVILELAATHQQALAVREISETELRGA
DEVWMTSSTKEILAITTLDGEPVGTGKPGPFGEQMWQWYQDFKNTVMRKG

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=299, Percent_Identity=29.7658862876254, Blast_Score=102, Evalue=2e-23,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 31936; Mature: 31805

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPYVADPVYLNGRFLPLAEAGISPLDRGFLYGDGVYEVIPVYSRRAFRIDEHVKRLQAT
CCCCCCCCEEECCEEEEHHHCCCCHHHCCEEECCCHHHHHHHHCCCEEHHHHHHHHHHHH
LDGIKLANPLTADGWKEVIAKLIETAPWDDQSIYLQVTRGADDKRDHAFPPASIPSTAFA
HHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHH
FASPLVTTPAEVRAKGVAAITVPDLRWSRCDLKVISLLANVLARQQAVDQGCAEALLIRD
HHCCCCCCCHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GFMKEGAASNIFIVKDGVLVAPPKTHLMLPGITYDVILELAATHQQALAVREISETELRG
CCCCCCCCCCEEEEECCEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ADEVWMTSSTKEILAITTLDGEPVGTGKPGPFGEQMWQWYQDFKNTVMRKG
CCCEEEECCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TPYVADPVYLNGRFLPLAEAGISPLDRGFLYGDGVYEVIPVYSRRAFRIDEHVKRLQAT
CCCCCCCEEECCEEEEHHHCCCCHHHCCEEECCCHHHHHHHHCCCEEHHHHHHHHHHHH
LDGIKLANPLTADGWKEVIAKLIETAPWDDQSIYLQVTRGADDKRDHAFPPASIPSTAFA
HHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHH
FASPLVTTPAEVRAKGVAAITVPDLRWSRCDLKVISLLANVLARQQAVDQGCAEALLIRD
HHCCCCCCCHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GFMKEGAASNIFIVKDGVLVAPPKTHLMLPGITYDVILELAATHQQALAVREISETELRG
CCCCCCCCCCEEEEECCEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ADEVWMTSSTKEILAITTLDGEPVGTGKPGPFGEQMWQWYQDFKNTVMRKG
CCCEEEECCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]