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The map label for this gene is cobD [H]

Identifier: 71905782

GI number: 71905782

Start: 153889

End: 154803

Strand: Reverse

Name: cobD [H]

Synonym: Daro_0140

Alternate gene names: 71905782

Gene position: 154803-153889 (Counterclockwise)

Preceding gene: 71905783

Following gene: 71905781

Centisome position: 3.44

GC content: 65.25

Gene sequence:

>915_bases
ATGATGATGCCCGAGGGTGCCCTTGCCATGCCGGCCGCCGCCCTGGTCGCCGTGCTGCTCGACCGCCTGCTCGGCGAAGT
GCGCCGTTTCCATCCGCTCGTTGGCTTTGGCAATCTGGCAGCCGCTATCGAAAAGCGCCTGAACAACCACCGTCTTGCCT
CCGGCCTGTTTGCCTGGGCGCTGGCTGTCCTGCCGTTTGTCGGACTGGCCTGGCTGGTACGCCCCGTCACTCCCTTGACC
GTCGACGTCGTTCTCCTCTACTTCGCACTCGGCGCCCAGAGCCTGTGCGAACACGCTGAAGCCATCGCCAAACCCTTGCG
GGAAGGGCATCTTGACGAGGCACGGCAGCGCGTCAGCTGGATTGTTTCTCGCGAGACATCGACGCTGGATGAATCCGGCA
TCGCCAAGGCCGGCGTCGAGTCGATACTGGAAAATGGCAACGATGCCATCTTCGGCACGCTGTTCTGGTTTGCCCTGCTC
GGCGGCCCCGGCGCCCTGCTCTTCCGGCTGGCCAATACGCTGGATGCAATGTGGGGCTACCGCACCGAGCGCTTCAACCT
GTTCGGCCGTTTTGCCGCCCGTTTTGACGACGCCTTGAACTGGGTTCCGGCCCGCCTGACGGCCCTGACTTATGCCTTGC
TCGGCCAGACCCGCAACGCCCTGGCCTGCTGGCGAACCCAGGCGCCGGGTTGGGACAGTCCGAATGCCGGGCCGGTAATG
TCGGCCGGGGCCGGCAGCCTTGGCGTGCAACTCGGCGGGGCGGCGATCTATCACGGGCAAGTGGAAAGCCGCCCACCACT
CGGTTCCGGTCCGCCCCCAGCGGCCGCCGACCTTGGCCGGGCGATCTCCCTGATCAGGCACAGCCTGTGGTTATGGTTGG
CCGTACTTTTTGCGATTGGACTCCTGCATGCTTGA

Upstream 100 bases:

>100_bases
ACGACCAGTGGGGCCAGGCCGATGTCGCCCGGAATGAGTCGCTGACCAACACGCTCGCCACCATGCAGGACGATATCCAG
CAACGGGGCATCTTCCCGAA

Downstream 100 bases:

>100_bases
ACACGGCGGCCGCCTGCGTGAAGCCGCGGCACACTACAACATCCCGCTGGAAAACTGGCTCGACTTGTCCACCGGCATCA
ATCCTGAGGCGTGGCCCGTC

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MMMPEGALAMPAAALVAVLLDRLLGEVRRFHPLVGFGNLAAAIEKRLNNHRLASGLFAWALAVLPFVGLAWLVRPVTPLT
VDVVLLYFALGAQSLCEHAEAIAKPLREGHLDEARQRVSWIVSRETSTLDESGIAKAGVESILENGNDAIFGTLFWFALL
GGPGALLFRLANTLDAMWGYRTERFNLFGRFAARFDDALNWVPARLTALTYALLGQTRNALACWRTQAPGWDSPNAGPVM
SAGAGSLGVQLGGAAIYHGQVESRPPLGSGPPPAAADLGRAISLIRHSLWLWLAVLFAIGLLHA

Sequences:

>Translated_304_residues
MMMPEGALAMPAAALVAVLLDRLLGEVRRFHPLVGFGNLAAAIEKRLNNHRLASGLFAWALAVLPFVGLAWLVRPVTPLT
VDVVLLYFALGAQSLCEHAEAIAKPLREGHLDEARQRVSWIVSRETSTLDESGIAKAGVESILENGNDAIFGTLFWFALL
GGPGALLFRLANTLDAMWGYRTERFNLFGRFAARFDDALNWVPARLTALTYALLGQTRNALACWRTQAPGWDSPNAGPVM
SAGAGSLGVQLGGAAIYHGQVESRPPLGSGPPPAAADLGRAISLIRHSLWLWLAVLFAIGLLHA
>Mature_304_residues
MMMPEGALAMPAAALVAVLLDRLLGEVRRFHPLVGFGNLAAAIEKRLNNHRLASGLFAWALAVLPFVGLAWLVRPVTPLT
VDVVLLYFALGAQSLCEHAEAIAKPLREGHLDEARQRVSWIVSRETSTLDESGIAKAGVESILENGNDAIFGTLFWFALL
GGPGALLFRLANTLDAMWGYRTERFNLFGRFAARFDDALNWVPARLTALTYALLGQTRNALACWRTQAPGWDSPNAGPVM
SAGAGSLGVQLGGAAIYHGQVESRPPLGSGPPPAAADLGRAISLIRHSLWLWLAVLFAIGLLHA

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 32616; Mature: 32616

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMMPEGALAMPAAALVAVLLDRLLGEVRRFHPLVGFGNLAAAIEKRLNNHRLASGLFAWA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LAVLPFVGLAWLVRPVTPLTVDVVLLYFALGAQSLCEHAEAIAKPLREGHLDEARQRVSW
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
IVSRETSTLDESGIAKAGVESILENGNDAIFGTLFWFALLGGPGALLFRLANTLDAMWGY
HHHHCCHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
RTERFNLFGRFAARFDDALNWVPARLTALTYALLGQTRNALACWRTQAPGWDSPNAGPVM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCC
SAGAGSLGVQLGGAAIYHGQVESRPPLGSGPPPAAADLGRAISLIRHSLWLWLAVLFAIG
CCCCCCCCCEECCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLHA
HHCC
>Mature Secondary Structure
MMMPEGALAMPAAALVAVLLDRLLGEVRRFHPLVGFGNLAAAIEKRLNNHRLASGLFAWA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LAVLPFVGLAWLVRPVTPLTVDVVLLYFALGAQSLCEHAEAIAKPLREGHLDEARQRVSW
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
IVSRETSTLDESGIAKAGVESILENGNDAIFGTLFWFALLGGPGALLFRLANTLDAMWGY
HHHHCCHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
RTERFNLFGRFAARFDDALNWVPARLTALTYALLGQTRNALACWRTQAPGWDSPNAGPVM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCC
SAGAGSLGVQLGGAAIYHGQVESRPPLGSGPPPAAADLGRAISLIRHSLWLWLAVLFAIG
CCCCCCCCCEECCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLHA
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]