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The map label for this gene is mrdA [H]

Identifier: 71905755

GI number: 71905755

Start: 129496

End: 131406

Strand: Reverse

Name: mrdA [H]

Synonym: Daro_0113

Alternate gene names: 71905755

Gene position: 131406-129496 (Counterclockwise)

Preceding gene: 71905756

Following gene: 71905754

Centisome position: 2.92

GC content: 58.56

Gene sequence:

>1911_bases
GTGGGTGATTTCAATAATCCGGAAGCCGATCTCGACCGTTTTCGGTTTCGGATCGGTTTTGCGGCGGTAACCGTCCTGCT
GGCCTTTGGCGTGCTGATTGGCCGCTTCGTCTGGCTACAGGTCATCCAGCACGATTTTTACCAGACCCGCGCCGAAGACA
ACCGTATTGCGCTGATCCCCATTGTGCCCAACCGTGGCGTCATCACCGACCGCAACGGTGTAGTGCTGGCCCACAACTAT
TCAGCCTTCACACTGGAAATCACGCCATCCCAAGCCGGTGACCTCGAGGCAACCATTGATTCGTTGGCAGAAGTCATCGA
TATCCAGCCCAAGGACCGCAAGCGCTTCAAGCGCCTGCTCGACGAAGCCAAGAATTTCGAATCGATCCCCATCCGCACCC
GCCTGACCGATGGCGAAGTCGCCAAGTTTGCGGCACACCGCTATCGTTTCCCCGGTGTCGAGGTCAAAGCCCGACTGTTC
CGGCAATACCCGTTGGCCAACATCGCCTCGCACGCCATTGGCTACATTGGGCGAATCACCGAACGCGACCTGAAATGGAT
CGAGGAGTCGGAAAAGCAGGCCAATTACAAGGGCACCGATCACGTCGGCAAGACAGGCCTCGAACAGCATTACGAATTTG
AACTGCACGGCGAGACCGGCTACGAGGAAGTCGAAATCGATGCCGGTGGCCGGGCCCTGCGCAGCCTGAAACGAATTCCC
CCGGTTTCCGGCAATAACCTGGCACTGACGCTCGATGCCAAATTGCAGGAAATCACCGAGCTAGCCTTCGGCGAGCGCAA
AGGCTCTCTTGTGGCCATCGACCCGTCAACCGGCGGCATTCTCGCGCTCGTCTCCAATCCGACCTACGACCCCAACCTGT
TCGTCGACGGTATTACACCGGACAACTGGAAGGAACTGAACGAGCACCCCAGCAAGCCAATGGTCAATCGAGCGATCAAC
GGAGCCTATCCGCCAGGCTCGACTTTCAAGCCCTTCCTTGCACTGGCTGCGCTGGAAATGGGCAAGCGCACGCCGAATCA
AACAATCAACGACCCCGGCTATTTCAACTTCGGCAATCACCAGTTCCGCGACGACAAGAAGGGCGGCCACGGGAGCGTGG
ACATGTACAAATCCATCGTCCATTCCTGCGACACCTACTATTACATGCTGGCCAACGACATGGGGATCGACAACATCGCC
AAGTTCATGGGCTCGCTCGGCCTCGGCCAGCGCACCGGCGTCGATCTCGGCAAGGATGATTCCGGTGAATCCAAAGGCGT
CCTGCCCTCGCAGGAGTGGAAGAAGCAACGTTTCAAAAAGCCTGAACAGCAGAGATGGTATGCCGGCGAGACGATCTCGA
TCGGCATCGGCCAAGGCTACAACGCTTACACGCCGATCCAGCTGGCCCAGGCCACCGCGACTATCGCCAATAATGGCGTA
ATGTTCCGGCCGCACCTGGTGCATTACATCATAGACACCAAGACGGGCGAAAAGCGGCAGGTCGAACCAAAGCCGATCCG
CGACCTCAATCTCAGGCCGCAGAATATCGATGTCATCCGCCGCGCCATGGTTGGCGTAAACAAGGAAGGCACGGGCGCCC
GGGCCTTTGCCGGCGCACCTTACGAATCAGCCGGCAAGACCGGCACCGCGCAGGTCTTCTCGCTAAAGGGTGCCCAGTAC
AAAGAAGGCGGCATGAAAAAAGAACTGCGCGACCACGCGCTGTTCATCGCCTATGCCCCGGCCGACAAACCGAAAATCGC
CTTGGCTGTACTGGTTGAGAACGGCGGCTTCGGCGCCCAGTCAGCCGCGCCTATCGCCCGCATGGTCATTGACTACTATC
TGCTCGGCAAGTTGCCGGGCGGTGCCGCCAAAGATGACCCAGCCGCCATCGAAGAGGATAGCGAGGAATGA

Upstream 100 bases:

>100_bases
GCTGCTCTGGATTCCCGCCACCTTCATCCTGCTCCTGCCGCAGTACCGGCCGGTCGAGCAAGACCCCAACCGCCCGATTT
GATGCTAAGGCAGGCTGCAC

Downstream 100 bases:

>100_bases
TCGACATCGTTGGCACCCTCCGCCGCAGTTGGCAGCAGTTGATCGCCCACATCGATTTTCCGCTGCTCTTCATCACGCTG
ACCATCATGGCGGTCGGCCT

Product: peptidoglycan glycosyltransferase

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 636; Mature: 635

Protein sequence:

>636_residues
MGDFNNPEADLDRFRFRIGFAAVTVLLAFGVLIGRFVWLQVIQHDFYQTRAEDNRIALIPIVPNRGVITDRNGVVLAHNY
SAFTLEITPSQAGDLEATIDSLAEVIDIQPKDRKRFKRLLDEAKNFESIPIRTRLTDGEVAKFAAHRYRFPGVEVKARLF
RQYPLANIASHAIGYIGRITERDLKWIEESEKQANYKGTDHVGKTGLEQHYEFELHGETGYEEVEIDAGGRALRSLKRIP
PVSGNNLALTLDAKLQEITELAFGERKGSLVAIDPSTGGILALVSNPTYDPNLFVDGITPDNWKELNEHPSKPMVNRAIN
GAYPPGSTFKPFLALAALEMGKRTPNQTINDPGYFNFGNHQFRDDKKGGHGSVDMYKSIVHSCDTYYYMLANDMGIDNIA
KFMGSLGLGQRTGVDLGKDDSGESKGVLPSQEWKKQRFKKPEQQRWYAGETISIGIGQGYNAYTPIQLAQATATIANNGV
MFRPHLVHYIIDTKTGEKRQVEPKPIRDLNLRPQNIDVIRRAMVGVNKEGTGARAFAGAPYESAGKTGTAQVFSLKGAQY
KEGGMKKELRDHALFIAYAPADKPKIALAVLVENGGFGAQSAAPIARMVIDYYLLGKLPGGAAKDDPAAIEEDSEE

Sequences:

>Translated_636_residues
MGDFNNPEADLDRFRFRIGFAAVTVLLAFGVLIGRFVWLQVIQHDFYQTRAEDNRIALIPIVPNRGVITDRNGVVLAHNY
SAFTLEITPSQAGDLEATIDSLAEVIDIQPKDRKRFKRLLDEAKNFESIPIRTRLTDGEVAKFAAHRYRFPGVEVKARLF
RQYPLANIASHAIGYIGRITERDLKWIEESEKQANYKGTDHVGKTGLEQHYEFELHGETGYEEVEIDAGGRALRSLKRIP
PVSGNNLALTLDAKLQEITELAFGERKGSLVAIDPSTGGILALVSNPTYDPNLFVDGITPDNWKELNEHPSKPMVNRAIN
GAYPPGSTFKPFLALAALEMGKRTPNQTINDPGYFNFGNHQFRDDKKGGHGSVDMYKSIVHSCDTYYYMLANDMGIDNIA
KFMGSLGLGQRTGVDLGKDDSGESKGVLPSQEWKKQRFKKPEQQRWYAGETISIGIGQGYNAYTPIQLAQATATIANNGV
MFRPHLVHYIIDTKTGEKRQVEPKPIRDLNLRPQNIDVIRRAMVGVNKEGTGARAFAGAPYESAGKTGTAQVFSLKGAQY
KEGGMKKELRDHALFIAYAPADKPKIALAVLVENGGFGAQSAAPIARMVIDYYLLGKLPGGAAKDDPAAIEEDSEE
>Mature_635_residues
GDFNNPEADLDRFRFRIGFAAVTVLLAFGVLIGRFVWLQVIQHDFYQTRAEDNRIALIPIVPNRGVITDRNGVVLAHNYS
AFTLEITPSQAGDLEATIDSLAEVIDIQPKDRKRFKRLLDEAKNFESIPIRTRLTDGEVAKFAAHRYRFPGVEVKARLFR
QYPLANIASHAIGYIGRITERDLKWIEESEKQANYKGTDHVGKTGLEQHYEFELHGETGYEEVEIDAGGRALRSLKRIPP
VSGNNLALTLDAKLQEITELAFGERKGSLVAIDPSTGGILALVSNPTYDPNLFVDGITPDNWKELNEHPSKPMVNRAING
AYPPGSTFKPFLALAALEMGKRTPNQTINDPGYFNFGNHQFRDDKKGGHGSVDMYKSIVHSCDTYYYMLANDMGIDNIAK
FMGSLGLGQRTGVDLGKDDSGESKGVLPSQEWKKQRFKKPEQQRWYAGETISIGIGQGYNAYTPIQLAQATATIANNGVM
FRPHLVHYIIDTKTGEKRQVEPKPIRDLNLRPQNIDVIRRAMVGVNKEGTGARAFAGAPYESAGKTGTAQVFSLKGAQYK
EGGMKKELRDHALFIAYAPADKPKIALAVLVENGGFGAQSAAPIARMVIDYYLLGKLPGGAAKDDPAAIEEDSEE

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=637, Percent_Identity=41.6012558869702, Blast_Score=508, Evalue=1e-145,
Organism=Escherichia coli, GI1786272, Length=621, Percent_Identity=25.1207729468599, Blast_Score=151, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 70304; Mature: 70172

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDFNNPEADLDRFRFRIGFAAVTVLLAFGVLIGRFVWLQVIQHDFYQTRAEDNRIALIP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
IVPNRGVITDRNGVVLAHNYSAFTLEITPSQAGDLEATIDSLAEVIDIQPKDRKRFKRLL
ECCCCCEEECCCCEEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
DEAKNFESIPIRTRLTDGEVAKFAAHRYRFPGVEVKARLFRQYPLANIASHAIGYIGRIT
HHHCCCCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ERDLKWIEESEKQANYKGTDHVGKTGLEQHYEFELHGETGYEEVEIDAGGRALRSLKRIP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCHHHHHHHHHCC
PVSGNNLALTLDAKLQEITELAFGERKGSLVAIDPSTGGILALVSNPTYDPNLFVDGITP
CCCCCCEEEEECHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEECCCCCCCCEEECCCCC
DNWKELNEHPSKPMVNRAINGAYPPGSTFKPFLALAALEMGKRTPNQTINDPGYFNFGNH
CCHHHHHCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
QFRDDKKGGHGSVDMYKSIVHSCDTYYYMLANDMGIDNIAKFMGSLGLGQRTGVDLGKDD
CCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCC
SGESKGVLPSQEWKKQRFKKPEQQRWYAGETISIGIGQGYNAYTPIQLAQATATIANNGV
CCCCCCCCCCHHHHHHHCCCCCHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCE
MFRPHLVHYIIDTKTGEKRQVEPKPIRDLNLRPQNIDVIRRAMVGVNKEGTGARAFAGAP
EECCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEECCCC
YESAGKTGTAQVFSLKGAQYKEGGMKKELRDHALFIAYAPADKPKIALAVLVENGGFGAQ
CHHCCCCCCEEEEEECCCCCCCCCCHHHHCCCEEEEEECCCCCCCEEEEEEEECCCCCCC
SAAPIARMVIDYYLLGKLPGGAAKDDPAAIEEDSEE
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GDFNNPEADLDRFRFRIGFAAVTVLLAFGVLIGRFVWLQVIQHDFYQTRAEDNRIALIP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
IVPNRGVITDRNGVVLAHNYSAFTLEITPSQAGDLEATIDSLAEVIDIQPKDRKRFKRLL
ECCCCCEEECCCCEEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
DEAKNFESIPIRTRLTDGEVAKFAAHRYRFPGVEVKARLFRQYPLANIASHAIGYIGRIT
HHHCCCCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ERDLKWIEESEKQANYKGTDHVGKTGLEQHYEFELHGETGYEEVEIDAGGRALRSLKRIP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCHHHHHHHHHCC
PVSGNNLALTLDAKLQEITELAFGERKGSLVAIDPSTGGILALVSNPTYDPNLFVDGITP
CCCCCCEEEEECHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEECCCCCCCCEEECCCCC
DNWKELNEHPSKPMVNRAINGAYPPGSTFKPFLALAALEMGKRTPNQTINDPGYFNFGNH
CCHHHHHCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
QFRDDKKGGHGSVDMYKSIVHSCDTYYYMLANDMGIDNIAKFMGSLGLGQRTGVDLGKDD
CCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCC
SGESKGVLPSQEWKKQRFKKPEQQRWYAGETISIGIGQGYNAYTPIQLAQATATIANNGV
CCCCCCCCCCHHHHHHHCCCCCHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCE
MFRPHLVHYIIDTKTGEKRQVEPKPIRDLNLRPQNIDVIRRAMVGVNKEGTGARAFAGAP
EECCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEECCCC
YESAGKTGTAQVFSLKGAQYKEGGMKKELRDHALFIAYAPADKPKIALAVLVENGGFGAQ
CHHCCCCCCEEEEEECCCCCCCCCCHHHHCCCEEEEEECCCCCCCEEEEEEEECCCCCCC
SAAPIARMVIDYYLLGKLPGGAAKDDPAAIEEDSEE
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]