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The map label for this gene is paaF [C]

Identifier: 71905732

GI number: 71905732

Start: 107219

End: 108004

Strand: Reverse

Name: paaF [C]

Synonym: Daro_0090

Alternate gene names: 71905732

Gene position: 108004-107219 (Counterclockwise)

Preceding gene: 71905733

Following gene: 71905731

Centisome position: 2.4

GC content: 65.27

Gene sequence:

>786_bases
ATGTTCACCGCACTAGAAATCGAACTGAACGGCCCGGTCGCCACGATCTGGATGAACCGGCCCGACCTGCACAACGCCTT
TGACGAAACCCTGATCGCCGAACTGACCGCCGCCTGCGTCGCGCTGGATCAGGATGACGACGTCCGCGTCGTCGTCCTCG
CCGGTCGCGGCAAGAGCTTCTCGGCCGGGGCCGACCTCAACTGGATGAAACGGGCCGCCAACAATGGTCTCGACGACAAC
CTGAACGATGCCCGGGCGCTGGCCAAGATGCTGCGCACGCTGGCTGAATTGAAGAAACCGACCATCGCCCGCATCCAGGG
CGCCGCCCTCGGCGGCGGGATGGGACTGGCCGCCGCCTGCGACATTGCTGTCGCTTCGAGCAAAGCGGTTTTCGCTACCT
CGGAGGTCAAGTTCGGCATCATCCCTTCGGCCATTTCGCCCTACGTGTTGCGCGCCATCGGCGCCCGCCAGGCAACCCGC
TACTTCCAGTCGGCCGAGCGCATCGACGCTTTCCGTGCCCGTGAGCTGGGGCTGGTCCATGAAGCCGTCAGCCCGGAAGA
CCTGGATGCCAAGATTCAGGAAATCGTCGCCACGCTGCTGCAAGGCGGCCCGCTCGCCCAGGCTGCCGCCAAGGACCTGA
TCCGCGCCGTCGATAGCCGACCGATCAACGACAACCTGGTCGAGGACACCGCCCACCGCATCGCCCACCTGCGCGCCACG
CCGGAAGCCCGCGAAGGCATCGCCGCCTTCCTCGACAAACGTCAGCCCAACTGGATTGGGGAATAA

Upstream 100 bases:

>100_bases
GAACCAGCCGGCGACCAATCAGGCCAGCCTGATCGCGGCAATGGAAGGCCAGCCGATGGCCGATCTGATCCGGCAACGAC
AACAATAATTGGAGCGAGAC

Downstream 100 bases:

>100_bases
GCCATGTTCACCAAGATTCTGATCGCCAACCGCGGGGAAATCGCCTGCCGCGTCATCAAGACCGCCCACCGCATGGGCAT
CCGCACCGTCGCCGTCTATT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MFTALEIELNGPVATIWMNRPDLHNAFDETLIAELTAACVALDQDDDVRVVVLAGRGKSFSAGADLNWMKRAANNGLDDN
LNDARALAKMLRTLAELKKPTIARIQGAALGGGMGLAAACDIAVASSKAVFATSEVKFGIIPSAISPYVLRAIGARQATR
YFQSAERIDAFRARELGLVHEAVSPEDLDAKIQEIVATLLQGGPLAQAAAKDLIRAVDSRPINDNLVEDTAHRIAHLRAT
PEAREGIAAFLDKRQPNWIGE

Sequences:

>Translated_261_residues
MFTALEIELNGPVATIWMNRPDLHNAFDETLIAELTAACVALDQDDDVRVVVLAGRGKSFSAGADLNWMKRAANNGLDDN
LNDARALAKMLRTLAELKKPTIARIQGAALGGGMGLAAACDIAVASSKAVFATSEVKFGIIPSAISPYVLRAIGARQATR
YFQSAERIDAFRARELGLVHEAVSPEDLDAKIQEIVATLLQGGPLAQAAAKDLIRAVDSRPINDNLVEDTAHRIAHLRAT
PEAREGIAAFLDKRQPNWIGE
>Mature_261_residues
MFTALEIELNGPVATIWMNRPDLHNAFDETLIAELTAACVALDQDDDVRVVVLAGRGKSFSAGADLNWMKRAANNGLDDN
LNDARALAKMLRTLAELKKPTIARIQGAALGGGMGLAAACDIAVASSKAVFATSEVKFGIIPSAISPYVLRAIGARQATR
YFQSAERIDAFRARELGLVHEAVSPEDLDAKIQEIVATLLQGGPLAQAAAKDLIRAVDSRPINDNLVEDTAHRIAHLRAT
PEAREGIAAFLDKRQPNWIGE

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=250, Percent_Identity=32.4, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI31542718, Length=265, Percent_Identity=27.5471698113208, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI213417737, Length=204, Percent_Identity=26.9607843137255, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI157694516, Length=204, Percent_Identity=26.9607843137255, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4502327, Length=261, Percent_Identity=32.5670498084291, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI20127408, Length=188, Percent_Identity=28.7234042553192, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI45643119, Length=220, Percent_Identity=27.7272727272727, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI260275230, Length=220, Percent_Identity=27.7272727272727, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI260274832, Length=220, Percent_Identity=27.7272727272727, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI70995211, Length=139, Percent_Identity=31.6546762589928, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221136756, Length=195, Percent_Identity=24.6153846153846, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI221136753, Length=195, Percent_Identity=24.6153846153846, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI221307494, Length=195, Percent_Identity=24.6153846153846, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI157694520, Length=178, Percent_Identity=25.8426966292135, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI68989263, Length=186, Percent_Identity=28.494623655914, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1787659, Length=250, Percent_Identity=34.4, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI221142681, Length=267, Percent_Identity=31.0861423220974, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788597, Length=250, Percent_Identity=28.8, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1787660, Length=252, Percent_Identity=29.7619047619048, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI87082183, Length=257, Percent_Identity=26.4591439688716, Blast_Score=84, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=30.7692307692308, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17554946, Length=248, Percent_Identity=31.0483870967742, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17540714, Length=208, Percent_Identity=32.2115384615385, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=255, Percent_Identity=27.4509803921569, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17540306, Length=258, Percent_Identity=26.3565891472868, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17558304, Length=184, Percent_Identity=30.9782608695652, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=135, Percent_Identity=30.3703703703704, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25144276, Length=148, Percent_Identity=31.7567567567568, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17508951, Length=148, Percent_Identity=31.7567567567568, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17508953, Length=148, Percent_Identity=31.7567567567568, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=235, Percent_Identity=26.8085106382979, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17560910, Length=172, Percent_Identity=27.906976744186, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17536985, Length=139, Percent_Identity=30.9352517985612, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI20129971, Length=253, Percent_Identity=30.0395256916996, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24653477, Length=253, Percent_Identity=30.0395256916996, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24653139, Length=263, Percent_Identity=31.1787072243346, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24654903, Length=264, Percent_Identity=23.8636363636364, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI19922422, Length=251, Percent_Identity=23.9043824701195, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24650670, Length=179, Percent_Identity=29.608938547486, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27988; Mature: 27988

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTALEIELNGPVATIWMNRPDLHNAFDETLIAELTAACVALDQDDDVRVVVLAGRGKSF
CCEEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCC
SAGADLNWMKRAANNGLDDNLNDARALAKMLRTLAELKKPTIARIQGAALGGGMGLAAAC
CCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCHHHHH
DIAVASSKAVFATSEVKFGIIPSAISPYVLRAIGARQATRYFQSAERIDAFRARELGLVH
HHHHCCCCEEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAVSPEDLDAKIQEIVATLLQGGPLAQAAAKDLIRAVDSRPINDNLVEDTAHRIAHLRAT
HCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
PEAREGIAAFLDKRQPNWIGE
CHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MFTALEIELNGPVATIWMNRPDLHNAFDETLIAELTAACVALDQDDDVRVVVLAGRGKSF
CCEEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCC
SAGADLNWMKRAANNGLDDNLNDARALAKMLRTLAELKKPTIARIQGAALGGGMGLAAAC
CCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCHHHHH
DIAVASSKAVFATSEVKFGIIPSAISPYVLRAIGARQATRYFQSAERIDAFRARELGLVH
HHHHCCCCEEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAVSPEDLDAKIQEIVATLLQGGPLAQAAAKDLIRAVDSRPINDNLVEDTAHRIAHLRAT
HCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
PEAREGIAAFLDKRQPNWIGE
CHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA