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The map label for this gene is murB

Identifier: 71905686

GI number: 71905686

Start: 50775

End: 51788

Strand: Reverse

Name: murB

Synonym: Daro_0044

Alternate gene names: 71905686

Gene position: 51788-50775 (Counterclockwise)

Preceding gene: 71905688

Following gene: 71905684

Centisome position: 1.15

GC content: 61.83

Gene sequence:

>1014_bases
ATGAATCCCAGCACCAACGTCGACCTCACCCCATTCAACACCCTCGCCCTCCCCGGCCGCGCAGCGCGCTATCAAAAAGT
CACCGCGCCCGAGGCGTTGACCGCGCCCGAGCTGGTCAAGGAAAAACGCTTCATTCTCGGCGGCGGCAGCAATCTGGTGC
TGACCGGCGATTTCGATGGCCTGCTGCTGCACATGGCCATCCCCGGCAAACGACTGGTCAAGGAAGATGCCGAGGCGTGG
TTTATCGAAGCCGGCGCCGGCGAGAACTGGCATGACTTCGTGCAATGGACACTGACCCGCGGCTGGCCGGGCCTGGAAAA
CCTGAGCCTGATTCCCGGCACAGTTGGTGCCGCACCGATCCAGAATATTGGGGCCTATGGGCTGGAAGTCGCCGATTGCC
TGCATTCTGTCACCGGCTGGGATTTTGAAAAAAAAGCGCTGCTGACGATTGATCGGGACGATTGCCGTTTCGCCTACCGC
GACAGCCTGTTCAAGCAGCAAGGCTGGCATTTGAACGGTAGGATCGCCATCACCTCGGTGATTTTTCGCCTGGCCAAGGC
CTGGCAGCCGAACATGCGCTATGCCGACATCGCTCAGGAACTGGCGACACGAAAAATTGCCGCGCCAAGCGCTCAGGACA
TCGCCACGGCAGTCATCGCCGTCCGCCAGCGCAAGCTGCCCGACCCGGCAGTGACCCCCAATGCCGGCAGCTTCTTTCAC
AATCCGGTCGTTGAAGCCATACAGGCCGAAGCCCTGGCCGACGCTTACCCGACACTACCCCGCTACCCGCAGCCCGATGG
TCGGGTCAAACTGGCCGCCGGCTGGCTGATCGAACAGGCCGGCTGGAAGGGCAAGGCGCTCGGTCCGGTCGGCATGTACG
AAAAGCAGGCACTGGTATTGGTCAATCGCGGCGGCGCCACAGGCCAGGATGTGCAGCGAACCATGGCTGCCGTACAGGCT
GCCGTGCGGGAAAAATTCGCGGTCGAACTGACGCCGGAACCGATATTTCTCTGA

Upstream 100 bases:

>100_bases
TCCCCGGCCGCTGCCCGGGTCAAGCACCGATCTGCCCCCTGCCCGCCATAAACCAACAAGCAACTCCAAACCAGGTCAAA
AATCGACGAAAATCCTGCCT

Downstream 100 bases:

>100_bases
AGCAAGCTATTCAGCGGGTGTGTCGCTTGCCTTGACGAACAGACAGGCCGCGATGCCGGTGGCCACGGCATCGGCCATGC
GCGCCTGGCGCTCAGGATCA

Product: UDP-N-acetylmuramate dehydrogenase

Products: NA

Alternate protein names: UDP-N-acetylmuramate dehydrogenase

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MNPSTNVDLTPFNTLALPGRAARYQKVTAPEALTAPELVKEKRFILGGGSNLVLTGDFDGLLLHMAIPGKRLVKEDAEAW
FIEAGAGENWHDFVQWTLTRGWPGLENLSLIPGTVGAAPIQNIGAYGLEVADCLHSVTGWDFEKKALLTIDRDDCRFAYR
DSLFKQQGWHLNGRIAITSVIFRLAKAWQPNMRYADIAQELATRKIAAPSAQDIATAVIAVRQRKLPDPAVTPNAGSFFH
NPVVEAIQAEALADAYPTLPRYPQPDGRVKLAAGWLIEQAGWKGKALGPVGMYEKQALVLVNRGGATGQDVQRTMAAVQA
AVREKFAVELTPEPIFL

Sequences:

>Translated_337_residues
MNPSTNVDLTPFNTLALPGRAARYQKVTAPEALTAPELVKEKRFILGGGSNLVLTGDFDGLLLHMAIPGKRLVKEDAEAW
FIEAGAGENWHDFVQWTLTRGWPGLENLSLIPGTVGAAPIQNIGAYGLEVADCLHSVTGWDFEKKALLTIDRDDCRFAYR
DSLFKQQGWHLNGRIAITSVIFRLAKAWQPNMRYADIAQELATRKIAAPSAQDIATAVIAVRQRKLPDPAVTPNAGSFFH
NPVVEAIQAEALADAYPTLPRYPQPDGRVKLAAGWLIEQAGWKGKALGPVGMYEKQALVLVNRGGATGQDVQRTMAAVQA
AVREKFAVELTPEPIFL
>Mature_337_residues
MNPSTNVDLTPFNTLALPGRAARYQKVTAPEALTAPELVKEKRFILGGGSNLVLTGDFDGLLLHMAIPGKRLVKEDAEAW
FIEAGAGENWHDFVQWTLTRGWPGLENLSLIPGTVGAAPIQNIGAYGLEVADCLHSVTGWDFEKKALLTIDRDDCRFAYR
DSLFKQQGWHLNGRIAITSVIFRLAKAWQPNMRYADIAQELATRKIAAPSAQDIATAVIAVRQRKLPDPAVTPNAGSFFH
NPVVEAIQAEALADAYPTLPRYPQPDGRVKLAAGWLIEQAGWKGKALGPVGMYEKQALVLVNRGGATGQDVQRTMAAVQA
AVREKFAVELTPEPIFL

Specific function: Cell wall formation

COG id: COG0812

COG function: function code M; UDP-N-acetylmuramate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

Organism=Escherichia coli, GI1790407, Length=339, Percent_Identity=41.8879056047198, Blast_Score=247, Evalue=8e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURB_DECAR (Q47K28)

Other databases:

- EMBL:   CP000089
- RefSeq:   YP_283273.1
- ProteinModelPortal:   Q47K28
- SMR:   Q47K28
- STRING:   Q47K28
- GeneID:   3567806
- GenomeReviews:   CP000089_GR
- KEGG:   dar:Daro_0044
- NMPDR:   fig|159087.4.peg.148
- eggNOG:   COG0812
- HOGENOM:   HBG686573
- OMA:   GAAPMQN
- ProtClustDB:   CLSK2525617
- BioCyc:   DARO159087:DARO_0044-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00037
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR003170
- InterPro:   IPR011601
- InterPro:   IPR006094
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.90.78.10
- PANTHER:   PTHR21071
- TIGRFAMs:   TIGR00179

Pfam domain/function: PF01565 FAD_binding_4; PF02873 MurB_C; SSF56176 FAD-binding_2; SSF56194 MurB_C

EC number: =1.1.1.158

Molecular weight: Translated: 36760; Mature: 36760

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: PS51387 FAD_PCMH

Important sites: ACT_SITE 160-160 ACT_SITE 237-237 ACT_SITE 333-333

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPSTNVDLTPFNTLALPGRAARYQKVTAPEALTAPELVKEKRFILGGGSNLVLTGDFDG
CCCCCCEECCCCCEEECCCCHHHHHCCCCCCCCCCHHHHHHCEEEEECCCEEEEEECCCC
LLLHMAIPGKRLVKEDAEAWFIEAGAGENWHDFVQWTLTRGWPGLENLSLIPGTVGAAPI
EEEEEECCCHHHHHHCCCEEEEECCCCCCHHHHHEEEECCCCCCCCCCEECCCCCCCCCH
QNIGAYGLEVADCLHSVTGWDFEKKALLTIDRDDCRFAYRDSLFKQQGWHLNGRIAITSV
HHHCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHH
IFRLAKAWQPNMRYADIAQELATRKIAAPSAQDIATAVIAVRQRKLPDPAVTPNAGSFFH
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
NPVVEAIQAEALADAYPTLPRYPQPDGRVKLAAGWLIEQAGWKGKALGPVGMYEKQALVL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECEEEHHCCCCCCCCCCCCCCCCCEEEE
VNRGGATGQDVQRTMAAVQAAVREKFAVELTPEPIFL
EECCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCC
>Mature Secondary Structure
MNPSTNVDLTPFNTLALPGRAARYQKVTAPEALTAPELVKEKRFILGGGSNLVLTGDFDG
CCCCCCEECCCCCEEECCCCHHHHHCCCCCCCCCCHHHHHHCEEEEECCCEEEEEECCCC
LLLHMAIPGKRLVKEDAEAWFIEAGAGENWHDFVQWTLTRGWPGLENLSLIPGTVGAAPI
EEEEEECCCHHHHHHCCCEEEEECCCCCCHHHHHEEEECCCCCCCCCCEECCCCCCCCCH
QNIGAYGLEVADCLHSVTGWDFEKKALLTIDRDDCRFAYRDSLFKQQGWHLNGRIAITSV
HHHCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHH
IFRLAKAWQPNMRYADIAQELATRKIAAPSAQDIATAVIAVRQRKLPDPAVTPNAGSFFH
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
NPVVEAIQAEALADAYPTLPRYPQPDGRVKLAAGWLIEQAGWKGKALGPVGMYEKQALVL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECEEEHHCCCCCCCCCCCCCCCCCEEEE
VNRGGATGQDVQRTMAAVQAAVREKFAVELTPEPIFL
EECCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA